2max - Revision history

OCA at 06:59, 1 May 2024

2024-05-01T06:59:39Z

← Older revision		Revision as of 06:59, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAX FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [https://pdbe.org/2max PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [https://www.ebi.ac.uk/pdbsum/2max PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [https://pdbe.org/2max PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [https://www.ebi.ac.uk/pdbsum/2max PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).		[https://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Bacterial RNA polymerase is a large, multi-subunit enzyme responsible for transcription of genomic information. The C-terminal domain of the alpha subunit of RNA polymerase (alphaCTD) functions as a DNA and protein recognition element localizing the polymerase on certain promoter sequences and is essential in all bacteria. Although alphaCTD is part of RNA polymerase, it is thought to have once been a separate transcription factor, and its primary role is the recruitment of RNA polymerase to various promoters. Despite the conservation of the subunits of RNA polymerase among bacteria, the mechanisms of regulation of transcription vary significantly. We have determined the tertiary structure of Helicobacter pylori alphaCTD. It is larger than other structurally determined alphaCTDs due to an extra, highly amphipathic helix near the C-terminal end. Residues within this helix are highly conserved among varepsilon-proteobacteria. The surface of the domain that binds A/T rich DNA sequences is conserved and showed binding to DNA similar to alphaCTDs of other bacteria. Using several NikR dependent promoter sequences, we observed cooperative binding of H. pylori alphaCTD to NikR:DNA complexes. We also produced alphaCTD lacking the 19 C-terminal residues, which showed greatly decreased stability, but maintained the core domain structure and binding affinity to NikR:DNA at low temperatures. The modeling of H. pylori alphaCTD into the context of transcriptional complexes suggests that the additional amphipathic helix mediates interactions with transcriptional regulators.

	Helicobacter pylori RNA polymerase alpha-subunit C-terminal domain shows features unique to varepsilon-proteobacteria and binds NikR/DNA complexes.,Borin BN, Tang W, Krezel AM Protein Sci. 2014 Jan 17. doi: 10.1002/pro.2427. PMID:24442709<ref>PMID:24442709</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]		*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:30, 2 March 2023

2023-03-02T06:30:00Z

← Older revision		Revision as of 06:30, 2 March 2023
Line 1:		Line 1:

	==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==		==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==
	<StructureSection load='2max' size='340' side='right'caption='[[2max~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>		<StructureSection load='2max' size='340' side='right'caption='[[2max]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Campylobacter_pylori_j99 Campylobacter~~ pylori ~~j99~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAX FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [https://pdbe.org/2max PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [https://www.ebi.ac.uk/pdbsum/2max PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [https://pdbe.org/2max PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [https://www.ebi.ac.uk/pdbsum/2max PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).		[https://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Campylobacter~~ pylori ~~j99]]~~		[[Category: Helicobacter pylori J99]]
	~~[[Category: DNA-directed RNA polymerase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Borin~~, B N~~]]		[[Category: Borin BN]]
	[[Category: Krezel~~, A M]]~~		[[Category: Krezel AM]]
	~~[[Category: Dna-directed rna polymerase]]~~
	~~[[Category: Hp1293]]~~
	~~[[Category: Jhp1213]]~~
	~~[[Category: Nucleotidyltransferase]]~~
	~~[[Category: Rna polymerase alpha subunit]]~~
	~~[[Category: Rpoa]]~~
	~~[[Category: Transcription]]~~
	~~[[Category: Transferase~~]]

OCA at 10:06, 26 May 2021

2021-05-26T10:06:33Z

← Older revision		Revision as of 10:06, 26 May 2021
Line 1:		Line 1:

	==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==		==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==
	<StructureSection load='2max' size='340' side='right' caption='[[2max]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2max' size='340' side='right'caption='[[2max]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MAX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAX FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [~~http~~://pdbe.org/2max PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2max PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [https://pdbe.org/2max PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [https://www.ebi.ac.uk/pdbsum/2max PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).		[[https://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	[[Category: Campylobacter pylori j99]]		[[Category: Campylobacter pylori j99]]
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
			[[Category: Large Structures]]
	[[Category: Borin, B N]]		[[Category: Borin, B N]]
	[[Category: Krezel, A M]]		[[Category: Krezel, A M]]

OCA at 13:59, 15 November 2017

2017-11-15T13:59:14Z

← Older revision		Revision as of 13:59, 15 November 2017
Line 1:		Line 1:

	==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==		==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==
	<StructureSection load='2max' size='340' side='right' caption='[[2max]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2max' size='340' side='right' caption='[[2max]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [http://pdbe.org/2max PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [http://www.ebi.ac.uk/pdbsum/2max PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [http://pdbe.org/2max PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [http://www.ebi.ac.uk/pdbsum/2max PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2max ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[RNA polymerase\|RNA polymerase]]
	*[[Temp1\|Temp1]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:38, 10 September 2015

2015-09-10T21:38:36Z

← Older revision		Revision as of 21:38, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [http://www.ebi.ac.uk/pdbsum/2max PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [http://pdbe.org/2max PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [http://www.ebi.ac.uk/pdbsum/2max PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2max" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase\|RNA polymerase]]
			*[[Temp1\|Temp1]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:26, 25 December 2014

2014-12-25T06:26:10Z

← Older revision		Revision as of 06:26, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2max\| PDB=2max \| SCENE= }}~~		==NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori==
	===NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori===		<StructureSection load='2max' size='340' side='right' caption='[[2max]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	~~{{ABSTRACT_PUBMED_24442709}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2max]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MAX FirstGlance]. <br>
			</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">jhp_1213, rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2max FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2max OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2max RCSB], [http://www.ebi.ac.uk/pdbsum/2max PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RPOA_HELPJ RPOA_HELPJ]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Bacterial RNA polymerase is a large, multi-subunit enzyme responsible for transcription of genomic information. The C-terminal domain of the alpha subunit of RNA polymerase (alphaCTD) functions as a DNA and protein recognition element localizing the polymerase on certain promoter sequences and is essential in all bacteria. Although alphaCTD is part of RNA polymerase, it is thought to have once been a separate transcription factor, and its primary role is the recruitment of RNA polymerase to various promoters. Despite the conservation of the subunits of RNA polymerase among bacteria, the mechanisms of regulation of transcription vary significantly. We have determined the tertiary structure of Helicobacter pylori alphaCTD. It is larger than other structurally determined alphaCTDs due to an extra, highly amphipathic helix near the C-terminal end. Residues within this helix are highly conserved among varepsilon-proteobacteria. The surface of the domain that binds A/T rich DNA sequences is conserved and showed binding to DNA similar to alphaCTDs of other bacteria. Using several NikR dependent promoter sequences, we observed cooperative binding of H. pylori alphaCTD to NikR:DNA complexes. We also produced alphaCTD lacking the 19 C-terminal residues, which showed greatly decreased stability, but maintained the core domain structure and binding affinity to NikR:DNA at low temperatures. The modeling of H. pylori alphaCTD into the context of transcriptional complexes suggests that the additional amphipathic helix mediates interactions with transcriptional regulators.

	~~==Function==~~		Helicobacter pylori RNA polymerase alpha-subunit C-terminal domain shows features unique to varepsilon-proteobacteria and binds NikR/DNA complexes.,Borin BN, Tang W, Krezel AM Protein Sci. 2014 Jan 17. doi: 10.1002/pro.2427. PMID:24442709<ref>PMID:24442709</ref>
	~~[[http~~:/~~/www~~.~~uniprot~~.~~org/uniprot~~/~~RPOA_HELPJ RPOA_HELPJ]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).~~

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2max]] is~~ a ~~1 chain structure. Full experimental information is available from [http://oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=2MAX OCA].~~		</div>

	==~~Reference~~==		==See Also==
	~~<ref group~~=~~"xtra">PMID:024442709</ref>~~<references ~~group="xtra"~~/><~~references~~/>		*[[RNA polymerase\|RNA polymerase]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Campylobacter pylori j99]]
	[[Category: DNA-directed RNA polymerase]]		[[Category: DNA-directed RNA polymerase]]
	[[Category: Borin, B N.]]		[[Category: Borin, B N]]
	[[Category: Krezel, A M.]]		[[Category: Krezel, A M]]
	[[Category: Dna-directed rna polymerase]]		[[Category: Dna-directed rna polymerase]]
	[[Category: Hp1293]]		[[Category: Hp1293]]

OCA at 13:33, 12 March 2014

2014-03-12T13:33:14Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_2max|  PDB=2max  |  SCENE=  }}
-The entry 2max is ON HOLD  until Paper Publication
+==Function==
-Authors: Borin, B.N., Krezel, A.M.
+==About this Structure==
-Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori
+==Reference==

OCA at 19:34, 7 August 2013

2013-08-07T19:34:08Z

← Older revision		Revision as of 19:34, 7 August 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 2max is ON HOLD		The entry 2max is ON HOLD until Paper Publication

	Authors: Borin, B.N., Krezel, A.M.		Authors: Borin, B.N., Krezel, A.M.

	Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori		Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori

OCA: Protected "2max" [edit=sysop:move=sysop]

2013-08-01T03:52:01Z

Protected "2max" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:52, 1 August 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 2max is ON HOLD Authors: Borin, B.N., Krezel, A.M. Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobact...

2013-08-01T03:52:00Z

New page: '''Unreleased structure''' The entry 2max is ON HOLD Authors: Borin, B.N., Krezel, A.M. Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobact...

New page

'''Unreleased structure'''

The entry 2max is ON HOLD

Authors: Borin, B.N., Krezel, A.M.

Description: NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori