2mb0 - Revision history

OCA at 06:59, 1 May 2024

2024-05-01T06:59:41Z

← Older revision		Revision as of 06:59, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [https://pdbe.org/2mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [https://pdbe.org/2mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>		[https://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Regulation of SMN2 exon 7 splicing is crucial for the production of active SMN protein and the survival of Spinal Muscular Atrophy (SMA) patients. One of the most efficient activators of exon 7 inclusion is hnRNP G, which is recruited to the exon by Tra2-beta1. We report that in addition to the C-terminal region of hnRNP G, the RNA Recognition Motif (RRM) and the middle part of the protein containing the Arg-Gly-Gly (RGG) box are important for this function. To better understand the mode of action of hnRNP G in this context we determined the structure of its RRM bound to an SMN2 derived RNA. The RRM interacts with a 5'-AAN-3' motif and specifically recognizes the two consecutive adenines. By testing the effect of mutations in hnRNP G RRM and in its putative binding sites on the splicing of SMN2 exon 7, we show that it specifically binds to exon 7. This interaction is required for hnRNP G splicing activity and we propose its recruitment to a polyA tract located upstream of the Tra2-beta1 binding site. Finally, our data suggest that hnRNP G plays a major role in the recruitment of the Tra2-beta1/hnRNP G/SRSF9 trimeric complex to SMN2 exon 7.

	~~Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.,Moursy A, Allain FH, Clery A Nucleic Acids Res. 2014 Apr 4. PMID:24692659<ref>PMID:24692659</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2mb0" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:30, 2 March 2023

2023-03-02T06:30:10Z

← Older revision		Revision as of 06:30, 2 March 2023
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	==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==		==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==
	<StructureSection load='2mb0' size='340' side='right'caption='[[2mb0~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2mb0' size='340' side='right'caption='[[2mb0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [https://pdbe.org/2mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [https://pdbe.org/2mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>		[https://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Allain~~, F H.~~T]]		[[Category: Allain FH-T]]
	[[Category: Clery, A]]		[[Category: Clery A]]
	[[Category: Moursy, A]]		[[Category: Moursy A]]
	~~[[Category: Hnrnp g]]~~
	~~[[Category: Rrm]]~~
	~~[[Category: Sma]]~~
	~~[[Category: Smn]]~~
	~~[[Category: Splicing]]~~
	~~[[Category: Splicing-rna complex~~]]

OCA at 10:06, 26 May 2021

2021-05-26T10:06:36Z

← Older revision		Revision as of 10:06, 26 May 2021
Line 1:		Line 1:

	==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==		==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==
	<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mb0' size='340' side='right'caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [~~http~~://pdbe.org/2mb0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [https://pdbe.org/2mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>		[[https://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Allain, F H.T]]		[[Category: Allain, F H.T]]
	[[Category: Clery, A]]		[[Category: Clery, A]]

OCA at 22:29, 9 August 2018

2018-08-09T22:29:05Z

← Older revision		Revision as of 22:29, 9 August 2018
Line 1:		Line 1:

	==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==		==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==
	<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://pdbe.org/2mb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://pdbe.org/2mb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:30, 11 September 2015

2015-09-11T21:30:04Z

← Older revision		Revision as of 21:30, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://pdbe.org/2mb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2mb0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:58, 25 December 2014

2014-12-25T19:58:30Z

← Older revision		Revision as of 19:58, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Allain, F H.T.]]		[[Category: Allain, F H.T]]
	[[Category: Clery, A.]]		[[Category: Clery, A]]
	[[Category: Moursy, A.]]		[[Category: Moursy, A]]
	[[Category: Hnrnp g]]		[[Category: Hnrnp g]]
	[[Category: Rrm]]		[[Category: Rrm]]

OCA at 05:08, 25 June 2014

2014-06-25T05:08:27Z

← Older revision		Revision as of 05:08, 25 June 2014
Line 2:		Line 2:
	<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. <br>		<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MB0 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>
	<table>		<table>
Line 13:		Line 12:
	Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.,Moursy A, Allain FH, Clery A Nucleic Acids Res. 2014 Apr 4. PMID:24692659<ref>PMID:24692659</ref>		Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.,Moursy A, Allain FH, Clery A Nucleic Acids Res. 2014 Apr 4. PMID:24692659<ref>PMID:24692659</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 06:39, 14 May 2014

2014-05-14T06:39:32Z

← Older revision		Revision as of 06:39, 14 May 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2mb0\| PDB=2mb0 \| SCENE= }}~~		==Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'==
	===Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'===		<StructureSection load='2mb0' size='340' side='right' caption='[[2mb0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2mb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBMX, HNRPG, RBMXP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2mb0 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Regulation of SMN2 exon 7 splicing is crucial for the production of active SMN protein and the survival of Spinal Muscular Atrophy (SMA) patients. One of the most efficient activators of exon 7 inclusion is hnRNP G, which is recruited to the exon by Tra2-beta1. We report that in addition to the C-terminal region of hnRNP G, the RNA Recognition Motif (RRM) and the middle part of the protein containing the Arg-Gly-Gly (RGG) box are important for this function. To better understand the mode of action of hnRNP G in this context we determined the structure of its RRM bound to an SMN2 derived RNA. The RRM interacts with a 5'-AAN-3' motif and specifically recognizes the two consecutive adenines. By testing the effect of mutations in hnRNP G RRM and in its putative binding sites on the splicing of SMN2 exon 7, we show that it specifically binds to exon 7. This interaction is required for hnRNP G splicing activity and we propose its recruitment to a polyA tract located upstream of the Tra2-beta1 binding site. Finally, our data suggest that hnRNP G plays a major role in the recruitment of the Tra2-beta1/hnRNP G/SRSF9 trimeric complex to SMN2 exon 7.

	~~==Function==~~		Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.,Moursy A, Allain FH, Clery A Nucleic Acids Res. 2014 Apr 4. PMID:24692659<ref>PMID:24692659</ref>
	~~[[http://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation~~ of ~~pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from~~ the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA ~~polymerase II. Component~~ of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2~~. Represses the~~ splicing ~~of MAPT/Tau exon 10~~. ~~Binds preferentially to single-stranded 5'-CC[~~A~~/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer~~. ~~Binds non-specifically to pre-mRNAs~~. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:~~18541147</ref> <ref>PMID:19282290</ref>~~ <ref>PMID:~~21327109~~</ref>

	~~==About this Structure==~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
	~~[[2mb0]] is~~ a ~~2 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2MB0 OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
			[[Category: Human]]
	[[Category: Allain, F H.T.]]		[[Category: Allain, F H.T.]]
	[[Category: Clery, A.]]		[[Category: Clery, A.]]

OCA at 06:36, 9 April 2014

2014-04-09T06:36:25Z

← Older revision		Revision as of 06:36, 9 April 2014
Line 1:		Line 1:
	~~'''Unreleased~~ structure'''		{{STRUCTURE_2mb0\| PDB=2mb0 \| SCENE= }}
			===Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'===

	~~The entry 2mb0 is ON HOLD~~ ~~until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/RBMX_HUMAN RBMX_HUMAN]] RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.<ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:16707624</ref> <ref>PMID:18445477</ref> <ref>PMID:18541147</ref> <ref>PMID:19282290</ref> <ref>PMID:21327109</ref>

	~~Authors~~: ~~Moursy, A~~.~~, Allain, F~~.H.-~~T., Clery, A~~.		==About this Structure==
			[[2mb0]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB0 OCA].

	~~Description~~: ~~Solution structure of hnRNP G RRM in~~ complex ~~with the RNA 5'-AUCAAA-3'~~		==Reference==
			<references group="xtra"/><references/>
			[[Category: Allain, F H.T.]]
			[[Category: Clery, A.]]
			[[Category: Moursy, A.]]
			[[Category: Hnrnp g]]
			[[Category: Rrm]]
			[[Category: Sma]]
			[[Category: Smn]]
			[[Category: Splicing]]
			[[Category: Splicing-rna complex]]

OCA at 03:10, 20 September 2013

2013-09-20T03:10:25Z

← Older revision		Revision as of 03:10, 20 September 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 2mb0 is ON HOLD		The entry 2mb0 is ON HOLD until Paper Publication

	Authors: Moursy, A., Allain, F.H.-T., Clery, A.		Authors: Moursy, A., Allain, F.H.-T., Clery, A.

	Description: Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'		Description: Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'