2mc2 - Revision history

OCA at 01:11, 21 November 2024

2024-11-21T01:11:31Z

← Older revision		Revision as of 01:11, 21 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [https://pdbe.org/2mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [https://pdbe.org/2mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:30, 2 March 2023

2023-03-02T06:30:58Z

← Older revision		Revision as of 06:30, 2 March 2023
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	==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==		==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==
	<StructureSection load='2mc2' size='340' side='right'caption='[[2mc2~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2mc2' size='340' side='right'caption='[[2mc2]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</div></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [https://pdbe.org/2mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [https://pdbe.org/2mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>		[https://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Amador, F]]		[[Category: Amador F]]
	[[Category: Ikura, M]]		[[Category: Ikura M]]
	[[Category: Seabrook, G]]		[[Category: Seabrook G]]
	[[Category: Stathopulos, P]]		[[Category: Stathopulos P]]
	~~[[Category: Alpha2 helix]]~~
	~~[[Category: Metal transport]]~~
	~~[[Category: Ryanodine receptor]]~~
	~~[[Category: Type ii~~]]

OCA at 10:07, 26 May 2021

2021-05-26T10:07:12Z

← Older revision		Revision as of 10:07, 26 May 2021
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	==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==		==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==
	<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mc2' size='340' side='right'caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [~~http~~://pdbe.org/2mc2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [https://pdbe.org/2mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>		[[https://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
	*[[Ryanodine receptor\|Ryanodine receptor]]		*[[Ryanodine receptor 3D structures\|Ryanodine receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Amador, F]]		[[Category: Amador, F]]

OCA at 22:52, 9 August 2018

2018-08-09T22:52:12Z

← Older revision		Revision as of 22:52, 9 August 2018
Line 1:		Line 1:

	==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==		==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==
	<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://pdbe.org/2mc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://pdbe.org/2mc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mc2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 11:27, 11 September 2015

2015-09-11T11:27:08Z

← Older revision		Revision as of 11:27, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://pdbe.org/2mc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2mc2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ryanodine receptor\|Ryanodine receptor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Amador, F]]		[[Category: Amador, F]]
	[[Category: Ikura, M]]		[[Category: Ikura, M]]

OCA at 15:49, 25 December 2014

2014-12-25T15:49:45Z

← Older revision		Revision as of 15:49, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:12, 18 December 2014

2014-12-18T12:12:41Z

← Older revision		Revision as of 12:12, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2mc2~~\| ~~PDB~~=2mc2 \| ~~SCENE~~= }}		==X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A==
	===X-ray crystallography-solution NMR hybrid structure of ~~mouse RyR2~~ domain A~~===~~		<StructureSection load='2mc2' size='340' side='right' caption='[[2mc2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	~~{{ABSTRACT_PUBMED_23978697}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2mc2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MC2 FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3im5\|3im5]], [[4kei\|4kei]], [[4kej\|4kej]], [[4kek\|4kek]], [[3qr5\|3qr5]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ryr2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mc2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mc2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mc2 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Ryanodine receptors (RyRs) are large tetrameric calcium (Ca2+) release channels found on the sarcoplasmic reticulum that respond to dihydropyridine receptor activity through a direct conformational interaction and/or indirect Ca2+ sensitivity, propagating sarcoplasmic reticulum luminal Ca2+ release in the process of excitation-contraction coupling. There are three human RyR subtypes, and several debilitating diseases are linked to heritable mutations in RyR1 and RyR2 including malignant hypothermia, central core disease, catecholaminergic polymorphic ventricular tachycardia (CPVT) and arrhythmogenic right ventricular dysplasia type 2 (ARVD2). Despite the recent appreciation that many disease-associated mutations within the N-terminal RyRABC domains (i.e., residues 1-559) are located in the putative interfaces mediating tetrameric channel assembly, the precise structural and dynamical consequences of the mutations are not well understood. We used solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography to examine the effect of ARVD2-associated (i.e., R176Q) and CPVT-associated [i.e., P164S, R169Q and delta exon 3 (Delta3)] mutations on the structure and dynamics of RyR2A. Our solution NMR data exposed a mobile alpha-helix, unique to type 2; further, this alpha2 helix rescues the beta-strand lost in RyR2A Delta3 but remains dynamic in the hot-spot loop (HS-loop) P164S, R169Q and R176Q mutant proteins. Docking of our X-ray crystal/NMR hybrid structure into the RyR1 cryo-electron microscopy map revealed that this RyR2A alpha2 helix is in close proximity to dense "columns" projecting toward the channel pore. This is in contrast to the HS-loop mutations that cause structural changes largely localized to the intersubunit interface between adjacent ABC domains. Taken together, our data suggest that ARVD2 and CPVT mutations have at least two distinct structural consequences linked to channel dysfunction: perturbation of the HS-loop (i.e., domain A):domain B intersubunit interface and disruption of the communication between the N-terminal region and the channel domain.

	~~==Function==~~		Type 2 Ryanodine Receptor Domain A Contains a Unique and Dynamic alpha-Helix That Transitions to a beta-Strand in a Mutant Linked with a Heritable Cardiomyopathy.,Amador FJ, Kimlicka L, Stathopulos PB, Gasmi-Seabrook GM, Maclennan DH, Van Petegem F, Ikura M J Mol Biol. 2013 Aug 23. pii: S0022-2836(13)00535-4. doi:, 10.1016/j.jmb.2013.08.015. PMID:23978697<ref>PMID:23978697</ref>
	~~[[http://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(~~2~~+) from the sarcoplasmic reticulum into the cytoplasm~~ and ~~thereby plays~~ a ~~key role~~ in ~~triggering cardiac muscle contraction~~. ~~Aberrant channel activation can lead to cardiac arrhythmia~~. ~~In cardiac myocytes, calcium release is triggered by increased Ca~~(2+) ~~levels due to activation of the L~~-~~type calcium channel CACNA1C~~. ~~The calcium channel activity is modulated by formation of heterotetramers with RYR3~~. ~~Required for cellular calcium ion homeostasis~~. ~~Required for embryonic heart development~~.~~<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>~~PMID:~~21098440</ref>~~ <ref>PMID:~~20431056~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2mc2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:023978697</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Amador, F.]]		[[Category: Amador, F]]
	[[Category: Ikura, M.]]		[[Category: Ikura, M]]
	[[Category: Seabrook, G.]]		[[Category: Seabrook, G]]
	[[Category: Stathopulos, P.]]		[[Category: Stathopulos, P]]
	[[Category: Alpha2 helix]]		[[Category: Alpha2 helix]]
	[[Category: Metal transport]]		[[Category: Metal transport]]
	[[Category: Ryanodine receptor]]		[[Category: Ryanodine receptor]]
	[[Category: Type ii]]		[[Category: Type ii]]

OCA at 05:25, 23 October 2013

2013-10-23T05:25:43Z

← Older revision		Revision as of 05:25, 23 October 2013
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	===X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A===		===X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A===
	{{ABSTRACT_PUBMED_23978697}}		{{ABSTRACT_PUBMED_23978697}}

			==Function==
			[[http://www.uniprot.org/uniprot/RYR2_MOUSE RYR2_MOUSE]] Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.<ref>PMID:10473538</ref> <ref>PMID:9628868</ref> <ref>PMID:21098440</ref> <ref>PMID:20431056</ref>

	==About this Structure==		==About this Structure==

OCA at 05:26, 12 September 2013

2013-09-12T05:26:15Z

← Older revision		Revision as of 05:26, 12 September 2013
Line 1:		Line 1:
	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_2mc2\| PDB=2mc2 \| SCENE= }}
			===X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A===
			{{ABSTRACT_PUBMED_23978697}}

	~~The entry~~ 2mc2 is ~~ON HOLD~~		==About this Structure==
			[[2mc2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MC2 OCA].

	~~Authors~~: Amador, F., ~~Stathopulos, P~~., Seabrook, G., ~~Ikura, M~~.		==Reference==
			<ref group="xtra">PMID:023978697</ref><references group="xtra"/><references/>
	~~Description~~: ~~X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A~~		[[Category: Mus musculus]]
			[[Category: Amador, F.]]
			[[Category: Ikura, M.]]
			[[Category: Seabrook, G.]]
			[[Category: Stathopulos, P.]]
			[[Category: Alpha2 helix]]
			[[Category: Metal transport]]
			[[Category: Ryanodine receptor]]
			[[Category: Type ii]]

OCA at 09:19, 4 September 2013

2013-09-04T09:19:36Z

← Older revision		Revision as of 09:19, 4 September 2013
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	Authors: Amador, F., Stathopulos, P., Seabrook, G., Ikura, M.		Authors: Amador, F., Stathopulos, P., Seabrook, G., Ikura, M.

	Description: X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A.		Description: X-ray crystallography-solution NMR hybrid structure of mouse RyR2 domain A