2ml2 - Revision history

OCA at 06:07, 15 May 2024

2024-05-15T06:07:02Z

← Older revision		Revision as of 06:07, 15 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [https://pdbe.org/2ml2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [https://pdbe.org/2ml2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:00, 8 March 2023

2023-03-08T08:00:37Z

← Older revision		Revision as of 08:00, 8 March 2023
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	==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==		==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==
	<StructureSection load='2ml2' size='340' side='right'caption='[[2ml2~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2ml2' size='340' side='right'caption='[[2ml2]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_478 Atcc 478~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2pyg\|2pyg]], [[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</div></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [https://pdbe.org/2ml2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [https://pdbe.org/2ml2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ALGE6_AZOVI ALGE6_AZOVI]] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), producing a polymer with gel-forming capacity, required for the formation of the cyst coat.		[https://www.uniprot.org/uniprot/ALGE6_AZOVI ALGE6_AZOVI] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), producing a polymer with gel-forming capacity, required for the formation of the cyst coat.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 478~~]]		[[Category: Azotobacter vinelandii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Aachmann~~, F L~~]]		[[Category: Aachmann FL]]
	[[Category: Buchinger, E]]		[[Category: Buchinger E]]
	[[Category: Wimmer, R]]		[[Category: Wimmer R]]
	~~[[Category: Alginate c-5 epimerase]]~~
	~~[[Category: Isomerase]]~~
	~~[[Category: Mannuronan c-5 epimerase]]~~
	~~[[Category: R-module~~]]

OCA at 15:16, 2 June 2021

2021-06-02T15:16:40Z

← Older revision		Revision as of 15:16, 2 June 2021
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	==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==		==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==
	<StructureSection load='2ml2' size='340' side='right' caption='[[2ml2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ml2' size='340' side='right'caption='[[2ml2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_478 Atcc 478]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_478 Atcc 478]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyg\|2pyg]], [[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2pyg\|2pyg]], [[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [~~http~~://pdbe.org/2ml2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [https://pdbe.org/2ml2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ALGE6_AZOVI ALGE6_AZOVI]] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), producing a polymer with gel-forming capacity, required for the formation of the cyst coat.		[[https://www.uniprot.org/uniprot/ALGE6_AZOVI ALGE6_AZOVI]] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), producing a polymer with gel-forming capacity, required for the formation of the cyst coat.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 478]]		[[Category: Atcc 478]]
			[[Category: Large Structures]]
	[[Category: Aachmann, F L]]		[[Category: Aachmann, F L]]
	[[Category: Buchinger, E]]		[[Category: Buchinger, E]]

OCA at 18:57, 1 August 2018

2018-08-01T18:57:46Z

← Older revision		Revision as of 18:57, 1 August 2018
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_478 Atcc 478]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_478 Atcc 478]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyg\|2pyg]], [[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://pdbe.org/2ml2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://pdbe.org/2ml2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>

OCA at 07:18, 13 December 2017

2017-12-13T07:18:06Z

← Older revision		Revision as of 07:18, 13 December 2017
Line 1:		Line 1:

	==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==		==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==
	<StructureSection load='2ml2' size='340' side='right' caption='[[2ml2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ml2' size='340' side='right' caption='[[2ml2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_478 Atcc 478]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]], [[2ml1\|2ml1]], [[2ml3\|2ml3]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://pdbe.org/2ml2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">algE6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 ATCC 478])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://pdbe.org/2ml2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ml2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 478]]
	[[Category: Aachmann, F L]]		[[Category: Aachmann, F L]]
	[[Category: Buchinger, E]]		[[Category: Buchinger, E]]

OCA at 00:45, 11 September 2015

2015-09-11T00:45:11Z

← Older revision		Revision as of 00:45, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://pdbe.org/2ml2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ml2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:47, 24 December 2014

2014-12-24T10:47:03Z

← Older revision		Revision as of 10:47, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ml2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ML2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ML2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pyh\|2pyh]], [[2agm\|2agm]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ml2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ml2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ml2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ml2 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ALGE6_AZOVI ALGE6_AZOVI]] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), producing a polymer with gel-forming capacity, required for the formation of the cyst coat.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The ~~17.7 kDa R2 module from~~ Azotobacter vinelandii ~~mannronan C5~~-~~epimerase AlgE6 has been isotopically labeled~~ ((13)C,(~~15)N~~) and ~~recombinantly expressed~~. ~~Here~~ we ~~report~~ the (1)H, (13)C, ~~(15)N resonance assignment~~ of ~~AlgE6R2~~.		The bacterium Azotobacter vinelandii produces a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1-7). These epimerases are responsible for the epimerization of beta-d-mannuronic acid (M) to alpha-l-guluronic acid (G) in alginate polymers. The epimerases display a modular structure composed of one or two catalytic A-modules and from one to seven R-modules having an activating effect on the A-module. In this study, we have determined the NMR structure of the three individual R-modules from AlgE6 (AR1R2R3) and the overall structure of both AlgE4 (AR) and AlgE6 using small angle x-ray scattering. Furthermore, the alginate binding ability of the R-modules of AlgE4 and AlgE6 has been studied with NMR and isothermal titration calorimetry. The AlgE6 R-modules fold into an elongated parallel beta-roll with a shallow, positively charged groove across the module. Small angle x-ray scattering analyses of AlgE4 and AlgE6 show an overall elongated shape with some degree of flexibility between the modules for both enzymes. Titration of the R-modules with defined alginate oligomers shows strong interaction between AlgE4R and both oligo-M and MG, whereas no interaction was detected between these oligomers and the individual R-modules from AlgE6. A combination of all three R-modules from AlgE6 shows weak interaction with long M-oligomers. Exchanging the R-modules between AlgE4 and AlgE6 resulted in a novel epimerase called AlgE64 with increased G-block forming ability compared with AlgE6.

	~~1H, 13C~~ and ~~15N resonances~~ of the ~~AlgE62 subunit~~ from Azotobacter vinelandii ~~mannuronan C5-epimerase~~.~~,Andreassen T~~, Buchinger E, Skjak-Braek G, ~~Valla S~~, Aachmann FL ~~Biomol NMR Assign~~. ~~2011 Oct~~;5(2):~~147~~-9. doi: 10.~~1007~~/~~s12104-010-9288-8~~. Epub ~~2010~~, ~~Dec 28~~. PMID:~~21188559~~<ref>PMID:~~21188559~~</ref>		Structural and Functional Characterization of the R-modules in Alginate C-5 Epimerases AlgE4 and AlgE6 from Azotobacter vinelandii.,Buchinger E, Knudsen DH, Behrens MA, Pedersen JS, Aarstad OA, Tondervik A, Valla S, Skjak-Braek G, Wimmer R, Aachmann FL J Biol Chem. 2014 Nov 7;289(45):31382-96. doi: 10.1074/jbc.M114.567008. Epub 2014, Sep 29. PMID:25266718<ref>PMID:25266718</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aachmann, F L.]]		[[Category: Aachmann, F L]]
	[[Category: Buchinger, E.]]		[[Category: Buchinger, E]]
	[[Category: Wimmer, R.]]		[[Category: Wimmer, R]]
	[[Category: Alginate c-5 epimerase]]		[[Category: Alginate c-5 epimerase]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Mannuronan c-5 epimerase]]		[[Category: Mannuronan c-5 epimerase]]
	[[Category: R-module]]		[[Category: R-module]]

OCA at 00:09, 2 October 2014

2014-10-02T00:09:41Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase==
-The entry 2ml2 is ON HOLD
+H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase.,Andreassen T, Buchinger E, Skjak-Braek G, Valla S, Aachmann FL Biomol NMR Assign. 2011 Oct;5(2):147-9. doi: 10.1007/s12104-010-9288-8. Epub 2010, Dec 28. PMID:21188559<ref>PMID:21188559</ref>
-Authors: Buchinger, E., Wimmer, R., Aachmann, F.L.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase
+== References ==

OCA: Protected "2ml2" [edit=sysop:move=sysop]

2014-03-03T16:04:39Z

Protected "2ml2" [edit=sysop:move=sysop]

← Older revision	Revision as of 16:04, 3 March 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 2ml2 is ON HOLD Authors: Buchinger, E., Wimmer, R., Aachmann, F.L. Description: Solution Structure of AlgE6R2 subunit from the Azotobacter vineland...

2014-03-03T16:04:37Z

New page: '''Unreleased structure''' The entry 2ml2 is ON HOLD Authors: Buchinger, E., Wimmer, R., Aachmann, F.L. Description: Solution Structure of AlgE6R2 subunit from the Azotobacter vineland...

New page

'''Unreleased structure'''

The entry 2ml2 is ON HOLD

Authors: Buchinger, E., Wimmer, R., Aachmann, F.L.

Description: Solution Structure of AlgE6R2 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase