2o7g - Revision history

OCA at 08:56, 25 October 2023

2023-10-25T08:56:37Z

← Older revision		Revision as of 08:56, 25 October 2023
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	<StructureSection load='2o7g' size='340' side='right'caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2o7g' size='340' side='right'caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7G FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2o8x\|2o8x]]~~<~~/div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~sigC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [https://pdbe.org/2o7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [https://www.ebi.ac.uk/pdbsum/2o7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [https://pdbe.org/2o7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [https://www.ebi.ac.uk/pdbsum/2o7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RPSC_MYCTU RPSC_MYCTU]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.		[https://www.uniprot.org/uniprot/RPSC_MYCTU RPSC_MYCTU] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Myctu~~]]		[[Category: Mycobacterium tuberculosis H37Rv]]
	[[Category: Gopal, B]]		[[Category: Gopal B]]
	[[Category: Joshi~~, A M~~]]		[[Category: Joshi AM]]
	[[Category: Thakur~~, K G]]~~		[[Category: Thakur KG]]
	~~[[Category: 10 element recognition domain]]~~
	~~[[Category: Sigma factor]]~~
	~~[[Category: Transcription]]~~
	~~[[Category: Transcription regulation~~]]

OCA at 16:02, 8 June 2021

2021-06-08T16:02:09Z

← Older revision		Revision as of 16:02, 8 June 2021
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	==Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis==		==Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis==
	<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2o7g' size='340' side='right'caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2O7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2o8x\|2o8x]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [~~http~~://pdbe.org/2o7g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2o7g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [https://pdbe.org/2o7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [https://www.ebi.ac.uk/pdbsum/2o7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RPSC_MYCTU RPSC_MYCTU]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.		[[https://www.uniprot.org/uniprot/RPSC_MYCTU RPSC_MYCTU]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7g_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Gopal, B]]		[[Category: Gopal, B]]

OCA at 08:53, 18 October 2017

2017-10-18T08:53:17Z

← Older revision		Revision as of 08:53, 18 October 2017
Line 1:		Line 1:

	==Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis==		==Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis==
	<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://pdbe.org/2o7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://pdbe.org/2o7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o7g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:00, 11 September 2015

2015-09-11T11:00:08Z

← Older revision		Revision as of 11:00, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1773 Mycobacterium tuberculosis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://pdbe.org/2o7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2o7g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mycobacterium tuberculosis~~]]		[[Category: Myctu]]
	[[Category: Gopal, B]]		[[Category: Gopal, B]]
	[[Category: Joshi, A M]]		[[Category: Joshi, A M]]

OCA at 15:33, 25 December 2014

2014-12-25T15:33:12Z

← Older revision		Revision as of 15:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7G FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RPSC_MYCTU RPSC_MYCTU]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Gopal, B.]]		[[Category: Gopal, B]]
	[[Category: Joshi, A M.]]		[[Category: Joshi, A M]]
	[[Category: Thakur, K G.]]		[[Category: Thakur, K G]]
	[[Category: -10 element recognition domain]]		[[Category: 10 element recognition domain]]
	[[Category: Sigma factor]]		[[Category: Sigma factor]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 18:27, 30 September 2014

2014-09-30T18:27:30Z

← Older revision		Revision as of 18:27, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2o7g~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis==
			<StructureSection load='2o7g' size='340' side='right' caption='[[2o7g]], [[Resolution\|resolution]] 2.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7G FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o8x\|2o8x]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sigC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7g RCSB], [http://www.ebi.ac.uk/pdbsum/2o7g PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7g_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			sigma factors are transcriptional regulatory proteins that bind to the RNA polymerase and dictate gene expression. The extracytoplasmic function (ECF) sigma factors govern the environment dependent regulation of transcription. ECF sigma factors have two domains sigma(2) and sigma(4) that recognize the -10 and -35 promoter elements. However, unlike the primary sigma factor sigma(A), the ECF sigma factors lack sigma(3), a region that helps in the recognition of the extended -10 element and sigma(1.1), a domain involved in the autoinhibition of sigma(A) in the absence of core RNA polymerase. Mycobacterium tuberculosis sigma(C) is an ECF sigma factor that is essential for the pathogenesis and virulence of M. tuberculosis in the mouse and guinea pig models of infection. However, unlike other ECF sigma factors, sigma(C) does not appear to have a regulatory anti-sigma factor located in the same operon. We also note that M. tuberculosis sigma(C) differs from the canonical ECF sigma factors as it has an N-terminal domain comprising of 126 amino acids that precedes the sigma(C)(2) and sigma(C)(4) domains. In an effort to understand the regulatory mechanism of this protein, the crystal structures of the sigma(C)(2) and sigma(C)(4) domains of sigma(C) were determined. These promoter recognition domains are structurally similar to the corresponding domains of sigma(A) despite the low sequence similarity. Fluorescence experiments using the intrinsic tryptophan residues of sigma(C)(2) as well as surface plasmon resonance measurements reveal that the sigma(C)(2) and sigma(C)(4) domains interact with each other. Mutational analysis suggests that the Pribnow box-binding region of sigma(C)(2) is involved in this interdomain interaction. Interaction between the promoter recognition domains in M. tuberculosis sigma(C) are thus likely to regulate the activity of this protein even in the absence of an anti-sigma factor.

	~~{{STRUCTURE_2o7g\| PDB=2o7g \| SCENE= }}~~		Structural and biophysical studies on two promoter recognition domains of the extra-cytoplasmic function sigma factor sigma(C) from Mycobacterium tuberculosis.,Thakur KG, Joshi AM, Gopal B J Biol Chem. 2007 Feb 16;282(7):4711-8. Epub 2006 Dec 4. PMID:17145760<ref>PMID:17145760</ref>

	~~===Crystal structure~~ of the ~~Pribnow Box recognition region of SigC from Mycobacterium tuberculosis===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17145760}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2o7g]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017145760<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Gopal, B.]]		[[Category: Gopal, B.]]

OCA: Protected "2o7g" [edit=sysop:move=sysop]

2013-01-07T10:42:52Z

Protected "2o7g" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:42, 7 January 2013
(No difference)

OCA at 10:42, 7 January 2013

2013-01-07T10:42:48Z

← Older revision		Revision as of 10:42, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2o7g.png\|left\|200px]]		[[Image:2o7g.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2o7g", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2o7g\| PDB=2o7g \| SCENE= }}		{{STRUCTURE_2o7g\| PDB=2o7g \| SCENE= }}

	===Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis===		===Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17145760}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17145760 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17145760}}		{{ABSTRACT_PUBMED_17145760}}

	==About this Structure==		==About this Structure==
	~~2O7G~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA].		[[2o7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17145760~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017145760</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Gopal, B.]]		[[Category: Gopal, B.]]
Line 30:		Line 18:
	[[Category: -10 element recognition domain]]		[[Category: -10 element recognition domain]]
	[[Category: Sigma factor]]		[[Category: Sigma factor]]
			[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:28:25 2009''~~

OCA at 18:28, 17 February 2009

2009-02-17T18:28:26Z

← Older revision		Revision as of 18:28, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2O7G is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA].		2O7G is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7G OCA].

	==Reference==		==Reference==
	Structural and biophysical studies on two promoter recognition domains of the extra-cytoplasmic function sigma factor sigma(C) from Mycobacterium tuberculosis., Thakur KG, Joshi AM, Gopal B, J Biol Chem. 2007 Feb 16;282(7):4711-8. Epub 2006 Dec 4. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17145760 17145760]~~		<ref group="xtra">PMID:17145760</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	~~[[Category: Single protein]]~~
	[[Category: Gopal, B.]]		[[Category: Gopal, B.]]
	[[Category: Joshi, A M.]]		[[Category: Joshi, A M.]]
Line 33:		Line 32:
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:22:~~36 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:28:25 2009''

OCA at 01:22, 29 July 2008

2008-07-29T01:22:41Z

← Older revision		Revision as of 01:22, 29 July 2008
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			[[Image:2o7g.png\|left\|200px]]

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	{{STRUCTURE_2o7g\| PDB=2o7g \| SCENE= }}		{{STRUCTURE_2o7g\| PDB=2o7g \| SCENE= }}

	~~'''~~Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis~~'''~~		===Crystal structure of the Pribnow Box recognition region of SigC from Mycobacterium tuberculosis===


	~~==Overview==~~		<!--
	~~sigma factors are transcriptional regulatory proteins that bind to the RNA polymerase and dictate gene expression.~~ The extracytoplasmic function (ECF) sigma factors govern the environment dependent regulation of transcription. ECF sigma factors have two domains sigma(2) and sigma(4) that recognize the -10 and -35 promoter elements. However, ~~unlike the primary sigma factor sigma(A)~~, the ECF sigma factors lack sigma(3), a region that helps in the recognition of the extended -10 element and sigma(1.1), a domain involved in the autoinhibition of sigma(A) in the absence of core RNA polymerase. Mycobacterium tuberculosis sigma(C) is an ECF sigma factor that is essential for the pathogenesis and virulence of M. tuberculosis in the mouse and guinea pig models of infection. However, unlike other ECF sigma factors, sigma(C) does not appear to ~~have a regulatory anti-sigma factor located in~~ the ~~same operon. We also note that M. tuberculosis sigma~~(~~C) differs from the canonical ECF sigma factors~~ as it ~~has an N-terminal domain comprising of 126 amino acids that precedes the sigma(C)(2) and sigma(C)(4) domains~~. ~~In an effort to understand the regulatory mechanism of this protein, the crystal structures of the sigma(C)(2) and sigma(C)(4) domains of sigma(C) were determined~~. ~~These promoter recognition domains are structurally similar to the corresponding domains of sigma(A~~) ~~despite~~ the ~~low sequence similarity~~. Fluorescence experiments using the intrinsic tryptophan residues of sigma(C)(2) as well as surface plasmon resonance measurements reveal that the sigma(C)(2) and sigma(C)(4) domains interact with each other. Mutational analysis suggests that the Pribnow box-binding region of sigma(C)(2) is involved in this interdomain interaction. Interaction between the promoter recognition domains in M. tuberculosis sigma(C) are thus likely to regulate the activity of this protein even in the absence of an anti-~~sigma factor.~~		The line below this paragraph, {{ABSTRACT_PUBMED_17145760}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17145760 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17145760}}

	==About this Structure==		==About this Structure==
Line 28:		Line 32:
	[[Category: Sigma factor]]		[[Category: Sigma factor]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 10~~:25:02 2008''
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