2o7m - Revision history

OCA at 08:56, 25 October 2023

2023-10-25T08:56:42Z

← Older revision		Revision as of 08:56, 25 October 2023
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	<StructureSection load='2o7m' size='340' side='right'caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2o7m' size='340' side='right'caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [https://pdbe.org/2o7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [https://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [https://pdbe.org/2o7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [https://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.		[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chlre~~]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Blanco~~, F J~~]]		[[Category: Blanco FJ]]
	[[Category: Montoya, G]]		[[Category: Montoya G]]
	[[Category: Padro, D]]		[[Category: Padro D]]
	[[Category: Paques, F]]		[[Category: Paques F]]
	[[Category: Prieto, J]]		[[Category: Prieto J]]
	[[Category: Redondo, P]]		[[Category: Redondo P]]
	~~[[Category: Dna]]~~
	~~[[Category: Homing endonuclease]]~~
	~~[[Category: Hydrolase~~]]

OCA at 07:50, 2 March 2022

2022-03-02T07:50:18Z

← Older revision		Revision as of 07:50, 2 March 2022
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	<StructureSection load='2o7m' size='340' side='right'caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2o7m' size='340' side='right'caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [~~http~~://pdbe.org/2o7m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [https://pdbe.org/2o7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [https://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.		[[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:58, 27 May 2020

2020-05-27T07:58:19Z

← Older revision		Revision as of 07:58, 27 May 2020
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	==The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold==		==The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold==
	<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2o7m' size='340' side='right'caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://pdbe.org/2o7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://pdbe.org/2o7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
			[[Category: Large Structures]]
	[[Category: Blanco, F J]]		[[Category: Blanco, F J]]
	[[Category: Montoya, G]]		[[Category: Montoya, G]]

OCA at 07:22, 22 August 2018

2018-08-22T07:22:20Z

← Older revision		Revision as of 07:22, 22 August 2018
Line 1:		Line 1:

	==The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold==		==The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold==
	<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://pdbe.org/2o7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://pdbe.org/2o7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o7m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7m_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:31, 7 February 2016

2016-02-07T15:31:23Z

← Older revision		Revision as of 15:31, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o7m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:49, 10 September 2015

2015-09-10T02:49:20Z

← Older revision		Revision as of 02:49, 10 September 2015
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	<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://pdbe.org/2o7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2o7m" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chlamydomonas reinhardtii~~]]		[[Category: Chlre]]
	[[Category: Blanco, F J]]		[[Category: Blanco, F J]]
	[[Category: Montoya, G]]		[[Category: Montoya, G]]

OCA at 16:19, 24 December 2014

2014-12-24T16:19:19Z

← Older revision		Revision as of 16:19, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Blanco, F J.]]		[[Category: Blanco, F J]]
	[[Category: Montoya, G.]]		[[Category: Montoya, G]]
	[[Category: Padro, D.]]		[[Category: Padro, D]]
	[[Category: Paques, F.]]		[[Category: Paques, F]]
	[[Category: Prieto, J.]]		[[Category: Prieto, J]]
	[[Category: Redondo, P.]]		[[Category: Redondo, P]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Homing endonuclease]]		[[Category: Homing endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:53, 29 September 2014

2014-09-29T05:53:16Z

← Older revision		Revision as of 05:53, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2o7m~~.~~png~~\|~~left~~\|~~200px~~]]		==The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold==
			<StructureSection load='2o7m' size='340' side='right' caption='[[2o7m]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O7M FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o7m RCSB], [http://www.ebi.ac.uk/pdbsum/2o7m PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/2o7m_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel perspectives for genome engineering in a wide range of fields, including gene therapy. A new crystal structure of the I-CreI dimer without DNA has allowed the comparison with the DNA-bound protein. The C-terminal loop displays a different conformation, which suggests its implication in DNA binding. A site-directed mutagenesis study in this region demonstrates that whereas the C-terminal helix is negligible for DNA binding, the final C-terminal loop is essential in DNA binding and cleavage. We have identified two regions that comprise the Ser138-Lys139 and Lys142-Thr143 pairs whose double mutation affect DNA binding in vitro and abolish cleavage in vivo. However, the mutation of only one residue in these sites allows DNA binding in vitro and cleavage in vivo. These findings demonstrate that the C-terminal loop of I-CreI endonuclease plays a fundamental role in its catalytic mechanism and suggest this novel site as a region to take into account for engineering new endonucleases with tailored specificity.

	~~{{STRUCTURE_2o7m\| PDB=2o7m \| SCENE= }}~~		The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage.,Prieto J, Redondo P, Padro D, Arnould S, Epinat JC, Paques F, Blanco FJ, Montoya G Nucleic Acids Res. 2007;35(10):3262-71. Epub 2007 Apr 22. PMID:17452357<ref>PMID:17452357</ref>

	~~===The C-terminal loop~~ of the ~~homing endonuclease I-CreI is essential for DNA binding and cleavage~~. ~~Identification~~ of ~~a novel site for specificity engineering in the I-CreI scaffold===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17452357}}~~

	~~==About this Structure==~~
	~~[[2o7m]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA].~~

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017452357<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Blanco, F J.]]		[[Category: Blanco, F J.]]

OCA at 07:04, 20 October 2012

2012-10-20T07:04:47Z

← Older revision		Revision as of 07:04, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2o7m]] is a 2 chain structure ~~of [[Endonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA].		[[2o7m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7M OCA].

	==See Also==		==See Also==

OCA: Protected "2o7m" [edit=sysop:move=sysop]

2012-07-25T15:12:17Z

Protected "2o7m" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:12, 25 July 2012
(No difference)