2p14 - Revision history

OCA at 10:52, 30 August 2023

2023-08-30T10:52:10Z

← Older revision		Revision as of 10:52, 30 August 2023
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	<StructureSection load='2p14' size='340' side='right'caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2p14' size='340' side='right'caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp.~~_d6~~ Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P14 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._D6 Bacillus sp. D6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P14 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[2ewf\|2ewf]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bspD6IR2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~127889 Bacillus sp. D6])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [https://pdbe.org/2p14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [https://www.ebi.ac.uk/pdbsum/2p14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p14 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [https://pdbe.org/2p14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [https://www.ebi.ac.uk/pdbsum/2p14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p14 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A3FEV8_9BACI A3FEV8_9BACI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. D6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Type II site-specific deoxyribonuclease]]~~		[[Category: Artyukh RI]]
	[[Category: Artyukh~~, R I~~]]		[[Category: Bartunik HD]]
	[[Category: Bartunik~~, H D~~]]		[[Category: Kachalova GS]]
	[[Category: Kachalova~~, G S~~]]		[[Category: Matvienko NI]]
	[[Category: Matvienko~~, N I~~]]		[[Category: Rogulin EA]]
	[[Category: Rogulin~~, E A~~]]		[[Category: Yunusova AK]]
	[[Category: Yunusova~~, A K~~]]		[[Category: Zheleznaya LA]]
	[[Category: Zheleznaya~~, L A]]~~
	~~[[Category: Heterodimeric restriction endonuclease]]~~
	~~[[Category: Hydrolase~~]]

OCA at 13:11, 9 June 2021

2021-06-09T13:11:30Z

← Older revision		Revision as of 13:11, 9 June 2021
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	==Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I==		==Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I==
	<StructureSection load='2p14' size='340' side='right' caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2p14' size='340' side='right'caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2P14 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P14 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ewf\|2ewf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ewf\|2ewf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [~~http~~://pdbe.org/2p14 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2p14 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2p14 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [https://pdbe.org/2p14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [https://www.ebi.ac.uk/pdbsum/2p14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p14 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
			[[Category: Large Structures]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
	[[Category: Artyukh, R I]]		[[Category: Artyukh, R I]]

OCA at 06:51, 22 August 2018

2018-08-22T06:51:11Z

← Older revision		Revision as of 06:51, 22 August 2018
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	==Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I==		==Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I==
	<StructureSection load='2p14' size='340' side='right' caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2p14' size='340' side='right' caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://pdbe.org/2p14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://pdbe.org/2p14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2p14 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p14_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p14_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p14 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:16, 11 September 2015

2015-09-11T08:16:00Z

← Older revision		Revision as of 08:16, 11 September 2015
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://pdbe.org/2p14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2p14" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:57, 19 January 2015

2015-01-19T14:57:26Z

← Older revision		Revision as of 14:57, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2P14 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2P14 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ewf\|2ewf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ewf\|2ewf]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
	[[Category: Artyukh, R I.]]		[[Category: Artyukh, R I]]
	[[Category: Bartunik, H D.]]		[[Category: Bartunik, H D]]
	[[Category: Kachalova, G S.]]		[[Category: Kachalova, G S]]
	[[Category: Matvienko, N I.]]		[[Category: Matvienko, N I]]
	[[Category: Rogulin, E A.]]		[[Category: Rogulin, E A]]
	[[Category: Yunusova, A K.]]		[[Category: Yunusova, A K]]
	[[Category: Zheleznaya, L A.]]		[[Category: Zheleznaya, L A]]
	[[Category: Heterodimeric restriction endonuclease]]		[[Category: Heterodimeric restriction endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 18:38, 30 September 2014

2014-09-30T18:38:15Z

← Older revision		Revision as of 18:38, 30 September 2014
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	[[Image:~~2p14~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I==
			<StructureSection load='2p14' size='340' side='right' caption='[[2p14]], [[Resolution\|resolution]] 1.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2p14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2P14 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ewf\|2ewf]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bspD6IR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127889 Bacillus sp. D6])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p14 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2p14 RCSB], [http://www.ebi.ac.uk/pdbsum/2p14 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p14_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The heterodimeric restriction endonuclease R.BspD6I from Bacillus species D6 recognizes a pseudosymmetric sequence and cuts both DNA strands outside the recognition sequence. The large subunit, Nt.BspD6I, acts as a type IIS site-specific monomeric nicking endonuclease. The isolated small subunit, ss.BspD6I, does not bind DNA and is not catalytically active. We solved the crystal structures of Nt.BspD6I and ss.BspD6I at high resolution. Nt.BspD6I consists of three domains, two of which exhibit structural similarity to the recognition and cleavage domains of FokI. ss.BspD6I has a fold similar to that of the cleavage domain of Nt.BspD6I, each containing a PD-(D/E)XK motif and a histidine as an additional putative catalytic residue. In contrast to the DNA-bound FokI structure, in which the cleavage domain is rotated away from the DNA, the crystal structure of Nt.BspD6I shows the recognition and cleavage domains in favorable orientations for interactions with DNA. Docking models of complexes of Nt.BspD6I and R.BspD6I with cognate DNA were constructed on the basis of structural similarity to individual domains of FokI, R.BpuJI and HindIII. A three-helix bundle forming an interdomain linker in Nt.BspD6I acts as a rigid spacer adjusting the orientations of the spatially separated domains to match the distance between the recognition and cleavage sites accurately.

	~~{{STRUCTURE_2p14\| PDB=2p14 \| SCENE= }}~~		Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.,Kachalova GS, Rogulin EA, Yunusova AK, Artyukh RI, Perevyazova TA, Matvienko NI, Zheleznaya LA, Bartunik HD J Mol Biol. 2008 Dec 12;384(2):489-502. Epub 2008 Sep 21. PMID:18835275<ref>PMID:18835275</ref>

	~~===Crystal structure~~ of ~~small subunit (R~~.~~BspD6I2)~~ of ~~the heterodimeric restriction endonuclease R~~.~~BspD6I===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_18835275}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2p14]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:018835275<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]

OCA: Protected "2p14" [edit=sysop:move=sysop]

2013-01-07T13:31:07Z

Protected "2p14" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:31, 7 January 2013
(No difference)

OCA at 13:31, 7 January 2013

2013-01-07T13:31:04Z

← Older revision		Revision as of 13:31, 7 January 2013
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	[[Image:2p14.png\|left\|200px]]		[[Image:2p14.png\|left\|200px]]

	~~<!--~~
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	{{STRUCTURE_2p14\| PDB=2p14 \| SCENE= }}		{{STRUCTURE_2p14\| PDB=2p14 \| SCENE= }}

	===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===		===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===


	~~<!--~~
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	{{ABSTRACT_PUBMED_18835275}}		{{ABSTRACT_PUBMED_18835275}}

	==About this Structure==		==About this Structure==
	~~2P14~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].		[[2p14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18835275~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018835275</ref><references group="xtra"/>
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
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	[[Category: Heterodimeric restriction endonuclease]]		[[Category: Heterodimeric restriction endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:26:54 2009''~~

OCA at 05:26, 25 June 2009

2009-06-25T05:26:55Z

← Older revision		Revision as of 05:26, 25 June 2009
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	===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===		===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===


			<!--
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			{{ABSTRACT_PUBMED_18835275}}

	==About this Structure==		==About this Structure==
	2P14 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].		2P14 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].

			==Reference==
			<ref group="xtra">PMID:18835275</ref><references group="xtra"/>
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 20~~:24:59 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:26:54 2009''

OCA at 17:20, 17 June 2009

2009-06-17T17:20:35Z

← Older revision		Revision as of 17:20, 17 June 2009
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	===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===		===Crystal structure of small subunit (R.BspD6I2) of the heterodimeric restriction endonuclease R.BspD6I===


	~~<!--~~
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	~~-->~~
	~~{{ABSTRACT_PUBMED_18835275}}~~

	==About this Structure==		==About this Structure==
	2P14 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].		2P14 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._d6 Bacillus sp. d6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P14 OCA].

	~~==Reference==~~
	~~<ref group="xtra">PMID:18835275</ref><references group="xtra"/>~~
	[[Category: Bacillus sp. d6]]		[[Category: Bacillus sp. d6]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 09:08:54 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 20:24:59 2009''