2pp3 - Revision history

OCA at 11:06, 30 August 2023

2023-08-30T11:06:18Z

← Older revision		Revision as of 11:06, 30 August 2023
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	<StructureSection load='2pp3' size='340' side='right'caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2pp3' size='340' side='right'caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LGT:L~~-~~GLUCARIC+ACID'>LGT</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height~~: ~~3em;~~'>~~[[2pp0\|2pp0]], [[2pp1\|2pp1]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [https://pdbe.org/2pp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [https://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [https://pdbe.org/2pp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [https://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/TAGAD_SALTY TAGAD_SALTY]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.<ref>PMID:17649980</ref>		[https://www.uniprot.org/uniprot/TAGAD_SALTY TAGAD_SALTY] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.<ref>PMID:17649980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Almo, S C~~]]		[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
	[[Category: ~~Fedorov, A A~~]]		[[Category: Almo SC]]
	[[Category: Fedorov~~, E V~~]]		[[Category: Fedorov AA]]
	[[Category: ~~Gerlt, J A~~]]		[[Category: Fedorov EV]]
	[[Category: ~~Yew, W S~~]]		[[Category: Gerlt JA]]
	[[Category: ~~Enolase superfamily]]~~		[[Category: Yew WS]]
	~~[[Category: L-talarate/galactarate dehydratase]]~~
	~~[[Category: Lyase~~]]

OCA at 18:27, 20 October 2021

2021-10-20T18:27:53Z

← Older revision		Revision as of 18:27, 20 October 2021
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	<StructureSection load='2pp3' size='340' side='right'caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2pp3' size='340' side='right'caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2pp0\|2pp0]], [[2pp1\|2pp1]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [~~http~~://pdbe.org/2pp3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [https://pdbe.org/2pp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [https://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/TAGAD_SALTY TAGAD_SALTY]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.<ref>PMID:17649980</ref>		[[https://www.uniprot.org/uniprot/TAGAD_SALTY TAGAD_SALTY]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.<ref>PMID:17649980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:56, 3 June 2020

2020-06-03T06:56:54Z

← Older revision		Revision as of 06:56, 3 June 2020
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	==Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate==		==Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate==
	<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2pp3' size='340' side='right'caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://pdbe.org/2pp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://pdbe.org/2pp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Fedorov, A A]]		[[Category: Fedorov, A A]]

OCA at 08:22, 29 August 2018

2018-08-29T08:22:07Z

← Older revision		Revision as of 08:22, 29 August 2018
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	==Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate==		==Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate==
	<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://pdbe.org/2pp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://pdbe.org/2pp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pp3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pp/2pp3_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pp/2pp3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:33, 9 February 2016

2016-02-09T14:33:20Z

← Older revision		Revision as of 14:33, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pp3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:11, 10 September 2015

2015-09-10T23:11:05Z

← Older revision		Revision as of 23:11, 10 September 2015
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	<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://pdbe.org/2pp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~Q8ZL58_SALTY Q8ZL58_SALTY~~]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.		[[http://www.uniprot.org/uniprot/TAGAD_SALTY TAGAD_SALTY]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.<ref>PMID:17649980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2pp3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]~~
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Fedorov, A A]]		[[Category: Fedorov, A A]]

OCA at 07:24, 25 December 2014

2014-12-25T07:24:43Z

← Older revision		Revision as of 07:24, 25 December 2014
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q8ZL58_SALTY Q8ZL58_SALTY]] Catalyzes the efficient dehydration of both L-talarate and galactarate to 5-keto-4-deoxy-D-glucarate. Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C]]
	[[Category: Fedorov, A A.]]		[[Category: Fedorov, A A]]
	[[Category: Fedorov, E V.]]		[[Category: Fedorov, E V]]
	[[Category: Gerlt, J A.]]		[[Category: Gerlt, J A]]
	[[Category: Yew, W S.]]		[[Category: Yew, W S]]
	[[Category: Enolase superfamily]]		[[Category: Enolase superfamily]]
	[[Category: L-talarate/galactarate dehydratase]]		[[Category: L-talarate/galactarate dehydratase]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 18:38, 30 September 2014

2014-09-30T18:38:57Z

← Older revision		Revision as of 18:38, 30 September 2014
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	[[Image:~~2pp3~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate==
			<StructureSection load='2pp3' size='340' side='right' caption='[[2pp3]], [[Resolution\|resolution]] 2.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2pp3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PP3 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGT:L-GLUCARIC+ACID'>LGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2pp0\|2pp0]], [[2pp1\|2pp1]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pp3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pp3 RCSB], [http://www.ebi.ac.uk/pdbsum/2pp3 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pp/2pp3_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse enolase superfamily, focusing our characterization on the protein encoded by the Salmonella typhimurium LT2 genome (GI:16766982; STM3697). Like the homologous mandelate racemase, l-fuconate dehydratase, and d-tartrate dehydratase, the active site of TalrD/GalrD contains a general acid/base Lys 197 at the end of the second beta-strand in the (beta/alpha)7beta-barrel domain, Asp 226, Glu 252, and Glu 278 as ligands for the essential Mg2+ at the ends of the third, fourth, and fifth beta-strands, a general acid/base His 328-Asp 301 dyad at the ends of the seventh and sixth beta-strands, and an electrophilic Glu 348 at the end of the eighth beta-strand. We discovered the function of STM3697 by screening a library of acid sugars; it catalyzes the efficient dehydration of both l-talarate (kcat = 2.1 s-1, kcat/Km = 9.1 x 10(3) M-1 s-1) and galactarate (kcat = 3.5 s-1, kcat/Km = 1.1 x 10(4) M-1 s-1). Because l-talarate is a previously unknown metabolite, we demonstrated that S. typhimurium LT2 can utilize l-talarate as carbon source. Insertional disruption of the gene encoding STM3697 abolishes this phenotype; this disruption also diminishes, but does not eliminate, the ability of the organism to utilize galactarate as carbon source. The dehydration of l-talarate is accompanied by competing epimerization to galactarate; little epimerization to l-talarate is observed in the dehydration of galactarate. On the basis of (1) structures of the wild type enzyme complexed with l-lyxarohydroxamate, an analogue of the enolate intermediate, and of the K197A mutant complexed with l-glucarate, a substrate for exchange of the alpha-proton, and (2) incorporation of solvent deuterium into galactarate in competition with dehydration, we conclude that Lys 197 functions as the galactarate-specific base and His 328 functions as the l-talarate-specific base. The epimerization of l-talarate to galactarate that competes with dehydration can be rationalized by partitioning of the enolate intermediate between dehydration (departure of the 3-OH group catalyzed by the conjugate acid of His 328) and epimerization (protonation on C2 by the conjugate acid of Lys 197). The promiscuous catalytic activities discovered for STM3697 highlight the evolutionary potential of a "conserved" active site architecture.

	~~{{STRUCTURE_2pp3\| PDB=2pp3 \| SCENE= }}~~		Evolution of enzymatic activities in the enolase superfamily: L-talarate/galactarate dehydratase from Salmonella typhimurium LT2.,Yew WS, Fedorov AA, Fedorov EV, Almo SC, Gerlt JA Biochemistry. 2007 Aug 21;46(33):9564-77. Epub 2007 Jul 25. PMID:17649980<ref>PMID:17649980</ref>

	~~===Crystal structure of L-talarate~~/~~galactarate dehydratase mutant K197A liganded with Mg and L-glucarate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17649980}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2pp3]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Salmonella_enterica_subsp~~.~~_enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017649980<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]

OCA: Protected "2pp3" [edit=sysop:move=sysop]

2013-01-07T10:55:33Z

Protected "2pp3" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:55, 7 January 2013
(No difference)

OCA at 10:55, 7 January 2013

2013-01-07T10:55:31Z

← Older revision		Revision as of 10:55, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2pp3.png\|left\|200px]]		[[Image:2pp3.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2pp3", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2pp3\| PDB=2pp3 \| SCENE= }}		{{STRUCTURE_2pp3\| PDB=2pp3 \| SCENE= }}

	===Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate===		===Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17649980}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17649980 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17649980}}		{{ABSTRACT_PUBMED_17649980}}

	==About this Structure==		==About this Structure==
	~~2PP3~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Salmonella_typhimurium~~ Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA].		[[2pp3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PP3 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17649980~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017649980</ref><references group="xtra"/>
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]
	[[Category: Fedorov, A A.]]		[[Category: Fedorov, A A.]]
Line 30:		Line 18:
	[[Category: Gerlt, J A.]]		[[Category: Gerlt, J A.]]
	[[Category: Yew, W S.]]		[[Category: Yew, W S.]]
	~~[[Category: Crystal structure]]~~
	[[Category: Enolase superfamily]]		[[Category: Enolase superfamily]]
	[[Category: L-talarate/galactarate dehydratase]]		[[Category: L-talarate/galactarate dehydratase]]
	[[Category: Lyase]]		[[Category: Lyase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:30:57 2009''~~