2pro - Revision history

OCA at 09:11, 21 February 2024

2024-02-21T09:11:38Z

← Older revision		Revision as of 09:11, 21 February 2024
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	<StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_29487 Atcc 29487~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [https://pdbe.org/2pro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [https://www.ebi.ac.uk/pdbsum/2pro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [https://pdbe.org/2pro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [https://www.ebi.ac.uk/pdbsum/2pro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PRLA_LYSEN PRLA_LYSEN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pro ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pro ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	While the majority of proteins fold rapidly and spontaneously to their native states, the extracellular bacterial protease alpha-lytic protease (alphaLP) has a t(1/2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1). AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction. Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9). In the absence of Pro, alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report the crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP. The C-shaped Pro surrounds the C-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier.

	~~Structure of alpha-lytic protease complexed with its pro region.,Sauter NK, Mau T, Rader SD, Agard DA Nat Struct Biol. 1998 Nov;5(11):945-50. PMID:9808037<ref>PMID:9808037</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2pro" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]		*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 29487]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Agard, D A~~]]		[[Category: Lysobacter enzymogenes]]
	[[Category: ~~Mau, T~~]]		[[Category: Agard DA]]
	[[Category: ~~Rader, S D~~]]		[[Category: Mau T]]
	[[Category: ~~Sauter, N K~~]]		[[Category: Rader SD]]
	[[Category: ~~Foldase]]~~		[[Category: Sauter NK]]
	~~[[Category: Pro region]]~~
	~~[[Category: Protein folding]]~~
	~~[[Category: Serine protease~~]]

OCA at 07:47, 9 March 2022

2022-03-09T07:47:12Z

← Older revision		Revision as of 07:47, 9 March 2022
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	<StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [~~http~~://pdbe.org/2pro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2pro PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [https://pdbe.org/2pro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [https://www.ebi.ac.uk/pdbsum/2pro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:57, 3 June 2020

2020-06-03T06:57:22Z

← Older revision		Revision as of 06:57, 3 June 2020
Line 1:		Line 1:

	==PRO REGION OF ALPHA-LYTIC PROTEASE==		==PRO REGION OF ALPHA-LYTIC PROTEASE==
	<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2pro' size='340' side='right'caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Alpha-lytic protease\|Alpha-lytic protease]]		*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 29487]]		[[Category: Atcc 29487]]
			[[Category: Large Structures]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Mau, T]]		[[Category: Mau, T]]

OCA at 08:23, 29 August 2018

2018-08-29T08:23:02Z

← Older revision		Revision as of 08:23, 29 August 2018
Line 1:		Line 1:

	==PRO REGION OF ALPHA-LYTIC PROTEASE==		==PRO REGION OF ALPHA-LYTIC PROTEASE==
	<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pro ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2pro_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2pro_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:19, 10 February 2016

2016-02-10T04:19:34Z

← Older revision		Revision as of 04:19, 10 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pro ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:19, 11 September 2015

2015-09-11T05:19:14Z

← Older revision		Revision as of 05:19, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lysobacter_enzymogenes Lysobacter enzymogenes~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://pdbe.org/2pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2pro" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lysobacter enzymogenes~~]]		[[Category: Atcc 29487]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Mau, T]]		[[Category: Mau, T]]

OCA at 15:02, 19 January 2015

2015-01-19T15:02:53Z

← Older revision		Revision as of 15:02, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
	[[Category: Agard, D A.]]		[[Category: Agard, D A]]
	[[Category: Mau, T.]]		[[Category: Mau, T]]
	[[Category: Rader, S D.]]		[[Category: Rader, S D]]
	[[Category: Sauter, N K.]]		[[Category: Sauter, N K]]
	[[Category: Foldase]]		[[Category: Foldase]]
	[[Category: Pro region]]		[[Category: Pro region]]
	[[Category: Protein folding]]		[[Category: Protein folding]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 18:39, 30 September 2014

2014-09-30T18:39:37Z

← Older revision		Revision as of 18:39, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2pro~~.~~png~~\|~~left~~\|~~200px~~]]		==PRO REGION OF ALPHA-LYTIC PROTEASE==
			<StructureSection load='2pro' size='340' side='right' caption='[[2pro]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PRO FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pro RCSB], [http://www.ebi.ac.uk/pdbsum/2pro PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2pro_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			While the majority of proteins fold rapidly and spontaneously to their native states, the extracellular bacterial protease alpha-lytic protease (alphaLP) has a t(1/2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1). AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction. Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9). In the absence of Pro, alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report the crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP. The C-shaped Pro surrounds the C-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier.

	~~{{STRUCTURE_2pro\| PDB=2pro \| SCENE= }}~~		Structure of alpha-lytic protease complexed with its pro region.,Sauter NK, Mau T, Rader SD, Agard DA Nat Struct Biol. 1998 Nov;5(11):945-50. PMID:9808037<ref>PMID:9808037</ref>

	~~===PRO REGION OF ALPHA-LYTIC PROTEASE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_9808037}}~~		==See Also==
			*[[Alpha-lytic protease\|Alpha-lytic protease]]
	==~~About this Structure~~==		== References ==
	[[~~2pro~~]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:009808037<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
	[[Category: Agard, D A.]]		[[Category: Agard, D A.]]

OCA: Protected "2pro" [edit=sysop:move=sysop]

2013-01-07T10:56:35Z

Protected "2pro" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:56, 7 January 2013
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OCA at 10:56, 7 January 2013

2013-01-07T10:56:32Z

← Older revision		Revision as of 10:56, 7 January 2013
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	[[Image:2pro.png\|left\|200px]]		[[Image:2pro.png\|left\|200px]]

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	{{STRUCTURE_2pro\| PDB=2pro \| SCENE= }}		{{STRUCTURE_2pro\| PDB=2pro \| SCENE= }}

	===PRO REGION OF ALPHA-LYTIC PROTEASE===		===PRO REGION OF ALPHA-LYTIC PROTEASE===


	~~<!--~~
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	{{ABSTRACT_PUBMED_9808037}}		{{ABSTRACT_PUBMED_9808037}}

	==About this Structure==		==About this Structure==
	~~2PRO~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA].		[[2pro]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PRO OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9808037~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009808037</ref><references group="xtra"/>
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
	[[Category: Agard, D A.]]		[[Category: Agard, D A.]]
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	[[Category: Protein folding]]		[[Category: Protein folding]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:22:42 2009''~~