2psa - Revision history

OCA at 07:47, 9 March 2022

2022-03-09T07:47:13Z

← Older revision		Revision as of 07:47, 9 March 2022
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	<StructureSection load='2psa' size='340' side='right'caption='[[2psa]]' scene=''>		<StructureSection load='2psa' size='340' side='right'caption='[[2psa]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2PSA FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2psa FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/2psa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2psa ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psa FirstGlance], [https://www.ebi.ac.uk/pdbsum/2psa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:57, 3 June 2020

2020-06-03T06:57:23Z

← Older revision		Revision as of 06:57, 3 June 2020
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	==KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE==		==KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE==
	<StructureSection load='2psa' size='340' side='right' caption='[[2psa]]' scene=''>		<StructureSection load='2psa' size='340' side='right'caption='[[2psa]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PSA FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PSA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2psa ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2psa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
			[[Category: Large Structures]]
	[[Category: Pellequer, J L]]		[[Category: Pellequer, J L]]

OCA at 08:59, 29 August 2018

2018-08-29T08:59:34Z

← Older revision		Revision as of 08:59, 29 August 2018
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSA FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2psa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:07, 11 February 2016

2016-02-11T11:07:31Z

← Older revision		Revision as of 11:07, 11 February 2016
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			{{Theoretical_model}}

	==KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE==		==KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE==
	<StructureSection load='2psa' size='340' side='right' caption='[[2psa]]' scene=''>		<StructureSection load='2psa' size='340' side='right' caption='[[2psa]]' scene=''>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Theoretical Model]]
	[[Category: Pellequer, J L]]		[[Category: Pellequer, J L]]

OCA at 06:41, 10 September 2015

2015-09-10T06:41:56Z

← Older revision		Revision as of 06:41, 10 September 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2psa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:39, 19 January 2015

2015-01-19T18:39:31Z

← Older revision		Revision as of 18:39, 19 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSA FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSA FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 01:04, 2 October 2014

2014-10-02T01:04:26Z

← Older revision		Revision as of 01:04, 2 October 2014
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	~~{{Theoretical_model}}~~		==KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE==
			<StructureSection load='2psa' size='340' side='right' caption='[[2psa]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSA FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psa FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psa PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BACKGROUND: The serine protease prostate-specific antigen (PSA) is a useful clinical marker for prostatic malignancy. PSA is a member of the kallikrein subgroup of the (chymo)trypsin serine protease family, but differs from the prototypical member of this subgroup, tissue kallikrein, in possessing a specificity more similar to that of chymotrypsin than trypsin. We report the use of two strategies, substrate phage display and iterative optimization of natural cleavage sites, to identify labile sequences for PSA cleavage. RESULTS: Iterative optimization and substrate phage display converged on the amino-acid sequence SS(Y/F)Y decreases S(G/S) as preferred subsite occupancy for PSA. These sequences were cleaved by PSA with catalytic efficiencies as high as 2200-3100 M-1 s-1, compared with values of 2-46 M-1 s-1 for peptides containing likely physiological target sequences of PSA from the protein semenogelin. Substrate residues that bind to secondary (non-S1) subsites have a critical role in defining labile substrates and can even cause otherwise disfavored amino acids to bind in the primary specificity (S1) pocket. CONCLUSION: The importance of secondary subsites in defining both the specificity and efficiency of cleavage suggests that substrate recognition by PSA is mediated by an extended binding site. Elucidation of preferred subsite occupancy allowed refinement of the structural model of PSA and should facilitate the development of more sensitive activity-based assays and the design of potent inhibitors.

	~~[[Image~~:~~2psa~~.~~png\|left\|200px]]~~		Substrate specificity of prostate-specific antigen (PSA).,Coombs GS, Bergstrom RC, Pellequer JL, Baker SI, Navre M, Smith MM, Tainer JA, Madison EL, Corey DR Chem Biol. 1998 Sep;5(9):475-88. PMID:9751643<ref>PMID:9751643</ref>

	~~{{STRUCTURE_2psa\| PDB=2psa \| SCENE= }}~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~===KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE===~~		== References ==
			<references/>
	~~{{ABSTRACT_PUBMED_9751643}}~~		__TOC__
			</StructureSection>
	==~~Reference~~==
	<~~ref group="xtra">PMID:009751643<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Pellequer, J L]]		[[Category: Pellequer, J L]]

OCA: Protected "2psa" [edit=sysop:move=sysop]

2012-10-21T10:04:25Z

Protected "2psa" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:04, 21 October 2012
(No difference)

OCA at 10:04, 21 October 2012

2012-10-21T10:04:21Z

← Older revision		Revision as of 10:04, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
	~~{{Seed}}~~
	[[Image:2psa.png\|left\|200px]]		[[Image:2psa.png\|left\|200px]]

	~~<!--~~
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	{{STRUCTURE_2psa\| PDB=2psa \| SCENE= }}		{{STRUCTURE_2psa\| PDB=2psa \| SCENE= }}

	===KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE===		===KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE===


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	{{ABSTRACT_PUBMED_9751643}}		{{ABSTRACT_PUBMED_9751643}}

	~~==About this Structure==~~
	~~Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSA OCA].~~

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9751643~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009751643</ref><references group="xtra"/>
	[[Category: Pellequer, J L]]		[[Category: Pellequer, J L]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 09:29:48 2010''~~

OCA at 06:29, 8 April 2010

2010-04-08T06:29:49Z

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-The entry 2PSA is a Theoretical Model titled 'KNOWLEDGE BASED MODEL OF PROSTATE SPECIFIC ANTIGEN (THEORETICAL MODEL) BOUND TO A CONSENSUS PEPTIDE SUBSTRATE'.
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