2pse - Revision history

OCA at 09:11, 21 February 2024

2024-02-21T09:11:41Z

← Older revision		Revision as of 09:11, 21 February 2024
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	<StructureSection load='2pse' size='340' side='right'caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2pse' size='340' side='right'caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pennatula_reniformis Pennatula~~ reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	~~<tr id='related'><td class="sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[2psd\|2psd]]~~, [[~~2psf~~\|~~2psf~~]]~~, [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~6136 Pennatula reniformis])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [https://pdbe.org/2pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [https://www.ebi.ac.uk/pdbsum/2pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [https://pdbe.org/2pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [https://www.ebi.ac.uk/pdbsum/2pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/LUCI_RENRE LUCI_RENRE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pse ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.

	Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.,Loening AM, Fenn TD, Gambhir SS J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:17980388<ref>PMID:17980388</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2pse" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Luciferase 3D structures\|Luciferase 3D structures]]		*[[Luciferase 3D structures\|Luciferase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Pennatula~~ reniformis]]		[[Category: Renilla reniformis]]
	~~[[Category: Renilla-luciferin 2-monooxygenase~~]]		[[Category: Fenn TD]]
	[[Category: Fenn~~, T D~~]]		[[Category: Gambhir SS]]
	[[Category: Gambhir~~, S S~~]]		[[Category: Loening AM]]
	[[Category: Loening~~, A M]]~~
	~~[[Category: Alpha/beta-hydrolase]]~~
	~~[[Category: Luciferase]]~~
	~~[[Category: Oxidoreductase~~]]

OCA at 18:28, 20 October 2021

2021-10-20T18:28:45Z

← Older revision		Revision as of 18:28, 20 October 2021
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	<StructureSection load='2pse' size='340' side='right'caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2pse' size='340' side='right'caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Renre Renre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pennatula_reniformis Pennatula reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 ~~RENRE~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Pennatula reniformis])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [https://pdbe.org/2pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [https://www.ebi.ac.uk/pdbsum/2pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [https://pdbe.org/2pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [https://www.ebi.ac.uk/pdbsum/2pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Pennatula reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
	~~[[Category: Renre]]~~
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]
	[[Category: Gambhir, S S]]		[[Category: Gambhir, S S]]

OCA at 15:31, 17 June 2021

2021-06-17T15:31:48Z

← Older revision		Revision as of 15:31, 17 June 2021
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	==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==		==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
	<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2pse' size='340' side='right'caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [~~http~~://pdbe.org/2pse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2pse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [https://pdbe.org/2pse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [https://www.ebi.ac.uk/pdbsum/2pse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Luciferase\|Luciferase]]		*[[Luciferase 3D structures\|Luciferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
	[[Category: Renre]]		[[Category: Renre]]

OCA at 08:22, 29 August 2018

2018-08-29T08:22:58Z

← Older revision		Revision as of 08:22, 29 August 2018
Line 1:		Line 1:

	==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==		==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
	<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://pdbe.org/2pse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://pdbe.org/2pse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pse ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2pse_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2pse_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:47, 11 September 2015

2015-09-11T09:47:15Z

← Older revision		Revision as of 09:47, 11 September 2015
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	<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Renilla_reniformis Renilla reniformis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 ~~Renilla reniformis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://pdbe.org/2pse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2pse" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Renilla reniformis]]~~
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
			[[Category: Renre]]
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]
	[[Category: Gambhir, S S]]		[[Category: Gambhir, S S]]

OCA at 17:24, 19 January 2015

2015-01-19T17:24:24Z

← Older revision		Revision as of 17:24, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
	[[Category: Fenn, T D.]]		[[Category: Fenn, T D]]
	[[Category: Gambhir, S S.]]		[[Category: Gambhir, S S]]
	[[Category: Loening, A M.]]		[[Category: Loening, A M]]
	[[Category: Alpha/beta-hydrolase]]		[[Category: Alpha/beta-hydrolase]]
	[[Category: Luciferase]]		[[Category: Luciferase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 09:37, 29 September 2014

2014-09-29T09:37:45Z

← Older revision		Revision as of 09:37, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2pse~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
			<StructureSection load='2pse' size='340' side='right' caption='[[2pse]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSE FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2psf\|2psf]], [[2psh\|2psh]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pse RCSB], [http://www.ebi.ac.uk/pdbsum/2pse PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2pse_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.

	~~{{STRUCTURE_2pse\| PDB=2pse \| SCENE= }}~~		Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.,Loening AM, Fenn TD, Gambhir SS J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:17980388<ref>PMID:17980388</ref>

	~~===Crystal Structures~~ of the ~~Luciferase and Green Fluorescent Protein from Renilla Reniformis===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17980388}}~~

	~~==About this Structure==~~
	~~[[2pse]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Renilla_reniformis Renilla reniformis]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2PSE OCA].~~

	==See Also==		==See Also==
	*[[Luciferase\|Luciferase]]		*[[Luciferase\|Luciferase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017980388<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]

OCA at 17:56, 20 October 2012

2012-10-20T17:56:41Z

← Older revision		Revision as of 17:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2pse]] is a 1 chain structure ~~of [[Luciferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA].		[[2pse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA].

	==See Also==		==See Also==

OCA: Protected "2pse" [edit=sysop:move=sysop]

2012-07-27T02:30:02Z

Protected "2pse" [edit=sysop:move=sysop]

← Older revision	Revision as of 02:30, 27 July 2012
(No difference)

OCA at 02:30, 27 July 2012

2012-07-27T02:30:00Z

← Older revision		Revision as of 02:30, 27 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2pse.png\|left\|200px]]		[[Image:2pse.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2pse", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2pse\| PDB=2pse \| SCENE= }}		{{STRUCTURE_2pse\| PDB=2pse \| SCENE= }}

	===Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis===		===Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17980388}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17980388 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17980388}}		{{ABSTRACT_PUBMED_17980388}}

	==About this Structure==		==About this Structure==
	~~2PSE~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA].		[[2pse]] is a 1 chain structure of [[Luciferase]] with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSE OCA].

			==See Also==
			*[[Luciferase\|Luciferase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17980388~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017980388</ref><references group="xtra"/>
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
Line 32:		Line 23:
	[[Category: Luciferase]]		[[Category: Luciferase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:59:46 2009''~~