2puh - Revision history

OCA at 11:09, 30 August 2023

2023-08-30T11:09:15Z

← Older revision		Revision as of 11:09, 30 August 2023
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	<StructureSection load='2puh' size='340' side='right'caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2puh' size='340' side='right'caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Burkholderia_xenovorans Burkholderia~~ xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PUH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paraburkholderia_xenovorans Paraburkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PUH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.82Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [https://pdbe.org/2puh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [https://www.ebi.ac.uk/pdbsum/2puh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2puh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [https://pdbe.org/2puh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [https://www.ebi.ac.uk/pdbsum/2puh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2puh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~BPHD_BURXL BPHD_BURXL]~~] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>		[https://www.uniprot.org/uniprot/BPHD_PARXL BPHD_PARXL] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Burkholderia xenovorans]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bhowmik, S~~]]		[[Category: Paraburkholderia xenovorans]]
	[[Category: ~~Bolin, J T~~]]		[[Category: Bhowmik S]]
	[[Category: ~~C-c bond hydrolase]]~~		[[Category: Bolin JT]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Structural genomic~~]]

OCA at 15:33, 17 June 2021

2021-06-17T15:33:03Z

← Older revision		Revision as of 15:33, 17 June 2021
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	==Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA==		==Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA==
	<StructureSection load='2puh' size='340' side='right' caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2puh' size='340' side='right'caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PUH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PUH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [~~http~~://pdbe.org/2puh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2puh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2puh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [https://pdbe.org/2puh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [https://www.ebi.ac.uk/pdbsum/2puh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2puh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>		[[https://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pu/2puh_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pu/2puh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
			[[Category: Large Structures]]
	[[Category: Bhowmik, S]]		[[Category: Bhowmik, S]]
	[[Category: Bolin, J T]]		[[Category: Bolin, J T]]

OCA at 09:33, 18 October 2017

2017-10-18T09:33:07Z

← Older revision		Revision as of 09:33, 18 October 2017
Line 1:		Line 1:

	==Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA==		==Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA==
	<StructureSection load='2puh' size='340' side='right' caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2puh' size='340' side='right' caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://pdbe.org/2puh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://pdbe.org/2puh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2puh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	[[Category: C-c bond hydrolase]]		[[Category: C-c bond hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
			[[Category: Structural genomic]]

OCA at 22:39, 8 February 2016

2016-02-08T22:39:48Z

← Older revision		Revision as of 22:39, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2puh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:01, 10 September 2015

2015-09-10T16:01:56Z

← Older revision		Revision as of 16:01, 10 September 2015
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://pdbe.org/2puh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2puh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:37, 25 December 2014

2014-12-25T02:37:11Z

← Older revision		Revision as of 02:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PUH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PUH FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
	[[Category: Bhowmik, S.]]		[[Category: Bhowmik, S]]
	[[Category: Bolin, J T.]]		[[Category: Bolin, J T]]
	[[Category: C-c bond hydrolase]]		[[Category: C-c bond hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 20:21, 30 September 2014

2014-09-30T20:21:20Z

← Older revision		Revision as of 20:21, 30 September 2014
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	[[Image:~~2puh~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA==
			<StructureSection load='2puh' size='340' side='right' caption='[[2puh]], [[Resolution\|resolution]] 1.82Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2puh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PUH FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[1j1i\|1j1i]], [[2d0d\|2d0d]], [[1c4x\|1c4x]], [[1u2e\|1u2e]], [[2pu5\|2pu5]], [[2pu6\|2pu6]], [[2pu7\|2pu7]], [[2puj\|2puj]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2puh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2puh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2puh RCSB], [http://www.ebi.ac.uk/pdbsum/2puh PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pu/2puh_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BphD of Burkholderia xenovorans LB400 catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to afford benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD). An enol-keto tautomerization has been proposed to precede hydrolysis via a gem-diol intermediate. The role of the canonical catalytic triad (Ser-112, His-265, Asp-237) in mediating these two half-reactions remains unclear. We previously reported that the BphD-catalyzed hydrolysis of HOPDA (lambda(max) is 434 nm for the free enolate) proceeds via an unidentified intermediate with a red-shifted absorption spectrum (lambda(max) is 492 nm) (Horsman, G. P., Ke, J., Dai, S., Seah, S. Y. K., Bolin, J. T., and Eltis, L. D. (2006) Biochemistry 45, 11071-11086). Here we demonstrate that the S112A variant generates and traps a similar intermediate (lambda(max) is 506 nm) with a similar rate, 1/tau approximately 500 s(-1). The crystal structure of the S112A:HOPDA complex at 1.8-A resolution identified this intermediate as the keto tautomer, (E)-2,6-dioxo-6-phenyl-hex-3-enoate. This keto tautomer did not accumulate in either the H265A or the S112A/H265A double variants, indicating that His-265 catalyzes tautomerization. Consistent with this role, the wild type and S112A enzymes catalyzed tautomerization of the product HPD, whereas H265A variants did not. This study thus identifies a keto intermediate, and demonstrates that the catalytic triad histidine catalyzes the tautomerization half-reaction, expanding the role of this residue from its purely hydrolytic function in other serine hydrolases. Finally, the S112A:HOPDA crystal structure is more consistent with hydrolysis occurring via an acyl-enzyme intermediate than a gem-diol intermediate as solvent molecules have poor access to C6, and the closest ordered water is 7 A away.

	~~{{STRUCTURE_2puh\| PDB=2puh \| SCENE= }}~~		The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad.,Horsman GP, Bhowmik S, Seah SY, Kumar P, Bolin JT, Eltis LD J Biol Chem. 2007 Jul 6;282(27):19894-904. Epub 2007 Apr 18. PMID:17442675<ref>PMID:17442675</ref>

	~~===Crystal Structure~~ of the ~~S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17442675}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2puh]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017442675<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
	[[Category: Bhowmik, S.]]		[[Category: Bhowmik, S.]]

OCA: Protected "2puh" [edit=sysop:move=sysop]

2013-01-07T15:25:01Z

Protected "2puh" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:25, 7 January 2013
(No difference)

OCA at 15:24, 7 January 2013

2013-01-07T15:24:57Z

← Older revision		Revision as of 15:24, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2puh.png\|left\|200px]]		[[Image:2puh.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2puh", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2puh\| PDB=2puh \| SCENE= }}		{{STRUCTURE_2puh\| PDB=2puh \| SCENE= }}

	===Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA===		===Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17442675}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17442675 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17442675}}		{{ABSTRACT_PUBMED_17442675}}

	==About this Structure==		==About this Structure==
	~~2PUH~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA].		[[2puh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17442675~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017442675</ref><references group="xtra"/>
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
	[[Category: Bhowmik, S.]]		[[Category: Bhowmik, S.]]
	[[Category: Bolin, J T.]]		[[Category: Bolin, J T.]]
	[[Category: C-c bond hydrolase]]		[[Category: C-c bond hydrolase]]
			[[Category: Hydrolase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 04:05:40 2009''~~

OCA at 02:05, 17 February 2009

2009-02-17T02:05:40Z

← Older revision		Revision as of 02:05, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2PUH is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA].		2PUH is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PUH OCA].

	==Reference==		==Reference==
	The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad., Horsman GP, Bhowmik S, Seah SY, Kumar P, Bolin JT, Eltis LD, J Biol Chem. 2007 Jul 6;282(27):19894-904. Epub 2007 Apr 18. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17442675 17442675]~~		<ref group="xtra">PMID:17442675</ref><references group="xtra"/>
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
	~~[[Category: Single protein]]~~
	[[Category: Bhowmik, S.]]		[[Category: Bhowmik, S.]]
	[[Category: Bolin, J T.]]		[[Category: Bolin, J T.]]
	[[Category: C-c bond hydrolase]]		[[Category: C-c bond hydrolase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 07~~:29:~~08 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:05:40 2009''