2rap - Revision history

OCA at 09:21, 21 February 2024

2024-02-21T09:21:17Z

← Older revision		Revision as of 09:21, 21 February 2024
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	<StructureSection load='2rap' size='340' side='right'caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2rap' size='340' side='right'caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [https://pdbe.org/2rap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [https://www.ebi.ac.uk/pdbsum/2rap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [https://pdbe.org/2rap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [https://www.ebi.ac.uk/pdbsum/2rap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN]] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref>		[https://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rap ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rap ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The small G protein Rap2A has been crystallized in complex with GDP, GTP and GTPgammaS. The Rap2A-GTP complex is the first structure of a small G protein with its natural ligand GTP. It shows that the hydroxyl group of Tyr32 forms a hydrogen bond with the gamma-phosphate of GTP and with Gly13. This interaction does not exist in the Rap2A-GTPgammaS complex. Tyr32 is conserved in many small G proteins, which probably also form this hydrogen bond with GTP. In addition, Tyr32 is structurally equivalent to a conserved arginine that binds GTP in trimeric G proteins. The actual participation of Tyr32 in GTP hydrolysis is not yet clear, but several possible roles are discussed. The conformational changes between the GDP and GTP complexes are located essentially in the switch I and II regions as described for the related oncoprotein H-Ras. However, the mobile segments vary in length and in the amplitude of movement. This suggests that even though similar regions might be involved in the GDP-GTP cycle of small G proteins, the details of the changes will be different for each G protein and will ensure the specificity of its interaction with a given set of cellular proteins.

	Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS.,Cherfils J, Menetrey J, Le Bras G, Janoueix-Lerosey I, de Gunzburg J, Garel JR, Auzat I EMBO J. 1997 Sep 15;16(18):5582-91. PMID:9312017<ref>PMID:9312017</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2rap" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cherfils, J]]		[[Category: Cherfils J]]
	[[Category: Lebras, G]]		[[Category: Lebras G]]
	[[Category: Menetrey, J]]		[[Category: Menetrey J]]
	~~[[Category: G protein]]~~
	~~[[Category: Gtp hydrolysis]]~~
	~~[[Category: Ra]]~~
	~~[[Category: Rap2a~~]]

OCA at 07:47, 16 March 2022

2022-03-16T07:47:05Z

← Older revision		Revision as of 07:47, 16 March 2022
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	<StructureSection load='2rap' size='340' side='right'caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2rap' size='340' side='right'caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [~~http~~://pdbe.org/2rap PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rap PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [https://pdbe.org/2rap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [https://www.ebi.ac.uk/pdbsum/2rap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN]] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref>		[[https://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN]] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:11, 10 June 2020

2020-06-10T07:11:10Z

← Older revision		Revision as of 07:11, 10 June 2020
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	==THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP==		==THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP==
	<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2rap' size='340' side='right'caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://pdbe.org/2rap PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://pdbe.org/2rap PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]
	[[Category: Lebras, G]]		[[Category: Lebras, G]]

OCA at 11:29, 5 September 2018

2018-09-05T11:29:27Z

← Older revision		Revision as of 11:29, 5 September 2018
Line 1:		Line 1:

	==THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP==		==THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP==
	<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://pdbe.org/2rap PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://pdbe.org/2rap PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rap ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2rap_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2rap_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 37:		Line 38:
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gtp hydrolysis]]		[[Category: Gtp hydrolysis]]
			[[Category: Ra]]
	[[Category: Rap2a]]		[[Category: Rap2a]]
	~~[[Category: Ra]]~~

OCA at 14:06, 9 February 2016

2016-02-09T14:06:02Z

← Older revision		Revision as of 14:06, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://pdbe.org/2rap PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rap ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2rap" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]
	[[Category: Lebras, G]]		[[Category: Lebras, G]]

OCA at 07:16, 25 December 2014

2014-12-25T07:16:46Z

← Older revision		Revision as of 07:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN]] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cherfils, J.]]		[[Category: Cherfils, J]]
	[[Category: Lebras, G.]]		[[Category: Lebras, G]]
	[[Category: Menetrey, J.]]		[[Category: Menetrey, J]]
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gtp hydrolysis]]		[[Category: Gtp hydrolysis]]
	[[Category: Rap2a]]		[[Category: Rap2a]]
	[[Category: Ra]]		[[Category: Ra]]

OCA at 18:29, 30 September 2014

2014-09-30T18:29:32Z

← Older revision		Revision as of 18:29, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2rap~~.~~png~~\|~~left~~\|~~200px~~]]		==THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP==
			<StructureSection load='2rap' size='340' side='right' caption='[[2rap]], [[Resolution\|resolution]] 2.60Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAP FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rap OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rap RCSB], [http://www.ebi.ac.uk/pdbsum/2rap PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2rap_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The small G protein Rap2A has been crystallized in complex with GDP, GTP and GTPgammaS. The Rap2A-GTP complex is the first structure of a small G protein with its natural ligand GTP. It shows that the hydroxyl group of Tyr32 forms a hydrogen bond with the gamma-phosphate of GTP and with Gly13. This interaction does not exist in the Rap2A-GTPgammaS complex. Tyr32 is conserved in many small G proteins, which probably also form this hydrogen bond with GTP. In addition, Tyr32 is structurally equivalent to a conserved arginine that binds GTP in trimeric G proteins. The actual participation of Tyr32 in GTP hydrolysis is not yet clear, but several possible roles are discussed. The conformational changes between the GDP and GTP complexes are located essentially in the switch I and II regions as described for the related oncoprotein H-Ras. However, the mobile segments vary in length and in the amplitude of movement. This suggests that even though similar regions might be involved in the GDP-GTP cycle of small G proteins, the details of the changes will be different for each G protein and will ensure the specificity of its interaction with a given set of cellular proteins.

	~~{{STRUCTURE_2rap\| PDB=2rap \| SCENE= }}~~		Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS.,Cherfils J, Menetrey J, Le Bras G, Janoueix-Lerosey I, de Gunzburg J, Garel JR, Auzat I EMBO J. 1997 Sep 15;16(18):5582-91. PMID:9312017<ref>PMID:9312017</ref>

	~~===THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9312017}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2rap]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:009312017<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cherfils, J.]]		[[Category: Cherfils, J.]]

OCA: Protected "2rap" [edit=sysop:move=sysop]

2013-01-07T18:23:05Z

Protected "2rap" [edit=sysop:move=sysop]

← Older revision	Revision as of 18:23, 7 January 2013
(No difference)

OCA at 18:23, 7 January 2013

2013-01-07T18:23:02Z

← Older revision		Revision as of 18:23, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2rap.png\|left\|200px]]		[[Image:2rap.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2rap", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2rap\| PDB=2rap \| SCENE= }}		{{STRUCTURE_2rap\| PDB=2rap \| SCENE= }}

	===THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP===		===THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_9312017}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 9312017 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_9312017}}		{{ABSTRACT_PUBMED_9312017}}

	==About this Structure==		==About this Structure==
	~~2RAP~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA].		[[2rap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9312017~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009312017</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cherfils, J.]]		[[Category: Cherfils, J.]]
Line 32:		Line 20:
	[[Category: Rap2a]]		[[Category: Rap2a]]
	[[Category: Ra]]		[[Category: Ra]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:21:19 2009''~~

OCA at 08:21, 17 February 2009

2009-02-17T08:21:20Z

← Older revision		Revision as of 08:21, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2RAP is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA].		2RAP is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAP OCA].

	==Reference==		==Reference==
	Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS., Cherfils J, Menetrey J, Le Bras G, Janoueix-Lerosey I, de Gunzburg J, Garel JR, Auzat I, EMBO J. 1997 Sep 15;16(18):5582-91. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9312017 9312017]~~		<ref group="xtra">PMID:9312017</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	~~[[Category: Single protein]]~~
	[[Category: Cherfils, J.]]		[[Category: Cherfils, J.]]
	[[Category: Lebras, G.]]		[[Category: Lebras, G.]]
Line 34:		Line 33:
	[[Category: Ra]]		[[Category: Ra]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 14~~:10:~~25 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:21:19 2009''