2raw - Revision history

OCA at 11:52, 30 August 2023

2023-08-30T11:52:28Z

← Older revision		Revision as of 11:52, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2raw' size='340' side='right'caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2raw' size='340' side='right'caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAW FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2rax\|2rax]]~~<~~/div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">BIRC5, API4, IAP4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [https://pdbe.org/2raw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [https://www.ebi.ac.uk/pdbsum/2raw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2raw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [https://pdbe.org/2raw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [https://www.ebi.ac.uk/pdbsum/2raw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2raw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/BIRC5_HUMAN BIRC5_HUMAN]] Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.<ref>PMID:10626797</ref> <ref>PMID:9859993</ref> <ref>PMID:12773388</ref> <ref>PMID:16322459</ref> <ref>PMID:16291752</ref> <ref>PMID:18591255</ref> <ref>PMID:20826784</ref> <ref>PMID:21536684</ref> [[https://www.uniprot.org/uniprot/BOREA_HUMAN BOREA_HUMAN]] Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.<ref>PMID:15260989</ref> <ref>PMID:15249581</ref> <ref>PMID:16571674</ref> <ref>PMID:18243099</ref>		[https://www.uniprot.org/uniprot/BIRC5_HUMAN BIRC5_HUMAN] Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.<ref>PMID:10626797</ref> <ref>PMID:9859993</ref> <ref>PMID:12773388</ref> <ref>PMID:16322459</ref> <ref>PMID:16291752</ref> <ref>PMID:18591255</ref> <ref>PMID:20826784</ref> <ref>PMID:21536684</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hymowitz~~, S G]]~~		[[Category: Hymowitz SG]]
	~~[[Category: Apoptosis]]~~
	~~[[Category: Bir]]~~
	~~[[Category: Cell cycle]]~~
	~~[[Category: Cell division]]~~
	~~[[Category: Centromere]]~~
	~~[[Category: Chromosomal passender complex]]~~
	~~[[Category: Chromosomal protein]]~~
	~~[[Category: Dasrab]]~~
	~~[[Category: Iap]]~~
	~~[[Category: Metal-binding]]~~
	~~[[Category: Mitosis]]~~
	~~[[Category: Nucleus]]~~
	~~[[Category: Phosphorylation]]~~
	~~[[Category: Protease inhibitor]]~~
	~~[[Category: Thiol protease inhibitor~~]]

OCA at 04:20, 2 July 2021

2021-07-02T04:20:05Z

← Older revision		Revision as of 04:20, 2 July 2021
Line 1:		Line 1:

	==Crystal structure of the Borealin-Survivin complex==		==Crystal structure of the Borealin-Survivin complex==
	<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2raw' size='340' side='right'caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rax\|2rax]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [~~http~~://pdbe.org/2raw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2raw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2raw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [https://pdbe.org/2raw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [https://www.ebi.ac.uk/pdbsum/2raw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2raw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/BIRC5_HUMAN BIRC5_HUMAN]] Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.<ref>PMID:10626797</ref> <ref>PMID:9859993</ref> <ref>PMID:12773388</ref> <ref>PMID:16322459</ref> <ref>PMID:16291752</ref> <ref>PMID:18591255</ref> <ref>PMID:20826784</ref> <ref>PMID:21536684</ref> [[~~http~~://www.uniprot.org/uniprot/BOREA_HUMAN BOREA_HUMAN]] Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.<ref>PMID:15260989</ref> <ref>PMID:15249581</ref> <ref>PMID:16571674</ref> <ref>PMID:18243099</ref>		[[https://www.uniprot.org/uniprot/BIRC5_HUMAN BIRC5_HUMAN]] Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.<ref>PMID:10626797</ref> <ref>PMID:9859993</ref> <ref>PMID:12773388</ref> <ref>PMID:16322459</ref> <ref>PMID:16291752</ref> <ref>PMID:18591255</ref> <ref>PMID:20826784</ref> <ref>PMID:21536684</ref> [[https://www.uniprot.org/uniprot/BOREA_HUMAN BOREA_HUMAN]] Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.<ref>PMID:15260989</ref> <ref>PMID:15249581</ref> <ref>PMID:16571674</ref> <ref>PMID:18243099</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Hymowitz, S G]]		[[Category: Hymowitz, S G]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]

OCA at 11:19, 5 September 2018

2018-09-05T11:19:57Z

← Older revision		Revision as of 11:19, 5 September 2018
Line 1:		Line 1:

	==Crystal structure of the Borealin-Survivin complex==		==Crystal structure of the Borealin-Survivin complex==
	<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://pdbe.org/2raw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://pdbe.org/2raw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2raw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2raw_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2raw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 32:

	==See Also==		==See Also==
			*[[Borealin\|Borealin]]
	*[[Survivin\|Survivin]]		*[[Survivin\|Survivin]]
	== References ==		== References ==

OCA at 17:40, 9 February 2016

2016-02-09T17:40:01Z

← Older revision		Revision as of 17:40, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://pdbe.org/2raw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2raw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2raw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Hymowitz, S G]]		[[Category: Hymowitz, S G]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]

OCA at 08:22, 25 December 2014

2014-12-25T08:22:58Z

← Older revision		Revision as of 08:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAW FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/BIRC5_HUMAN BIRC5_HUMAN]] Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.<ref>PMID:10626797</ref> <ref>PMID:9859993</ref> <ref>PMID:12773388</ref> <ref>PMID:16322459</ref> <ref>PMID:16291752</ref> <ref>PMID:18591255</ref> <ref>PMID:20826784</ref> <ref>PMID:21536684</ref> [[http://www.uniprot.org/uniprot/BOREA_HUMAN BOREA_HUMAN]] Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.<ref>PMID:15260989</ref> <ref>PMID:15249581</ref> <ref>PMID:16571674</ref> <ref>PMID:18243099</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hymowitz, S G.]]		[[Category: Hymowitz, S G]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Bir]]		[[Category: Bir]]

OCA at 19:33, 30 September 2014

2014-09-30T19:33:54Z

← Older revision		Revision as of 19:33, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2raw~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the Borealin-Survivin complex==
			<StructureSection load='2raw' size='340' side='right' caption='[[2raw]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RAW FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rax\|2rax]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2raw_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Survivin is a member of the IAP (inhibitor of apoptosis) protein family, defined in part by the presence of a zinc-binding baculoviral inhibitory repeat (BIR) domain. Most BIR domains bind short sequences beginning with alanine, and in this manner, they recognize and block the action of key targets in apoptotic pathways. However, Survivin binds only very weakly to typical IAP ligands. Unique features of Survivin are the long C-terminal helix following the BIR domain and a short segment (linking the helix and BIR domains) that mediates Survivin homodimerization. Despite this detailed knowledge of the structure of Survivin itself, there is a current lack of understanding about how Survivin recognizes cellular binding partners, and consequently, many questions about Survivin function remain unanswered. We determined two co-crystal structures of Survivin and a minimal binding fragment from the chromosomal passenger protein Borealin, a well validated functional interactor. The interaction between Survivin and Borealin involves extensive packing between the long C-terminal helix of Survivin and a long Borealin helix. Surprisingly, an additional important interaction occurs between the Survivin homodimerization interface and a short segment of Borealin. This segment both structurally mimics and displaces one Survivin monomer. The relevance of this unexpected interaction was tested by mutagenesis of two key Borealin residues. Mutant Borealin introduced into HeLa cells failed to localize properly during mitosis and also caused mislocalization of other chromosomal passenger proteins. This suggests that the mutant is dominant-negative and confirms the functional importance of the interaction surface identified in the crystal structures.

	~~{{STRUCTURE_2raw\| PDB=2raw \| SCENE= }}~~		The mitotic regulator Survivin binds as a monomer to its functional interactor Borealin.,Bourhis E, Hymowitz SG, Cochran AG J Biol Chem. 2007 Nov 30;282(48):35018-23. Epub 2007 Sep 19. PMID:17881355<ref>PMID:17881355</ref>

	~~===Crystal structure~~ of the ~~Borealin-Survivin complex===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_17881355}}~~		==See Also==
			*[[Survivin\|Survivin]]
	==~~About this Structure~~==		== References ==
	[[~~2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:017881355<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hymowitz, S G.]]		[[Category: Hymowitz, S G.]]

OCA: Protected "2raw" [edit=sysop:move=sysop]

2013-01-07T19:40:09Z

Protected "2raw" [edit=sysop:move=sysop]

← Older revision	Revision as of 19:40, 7 January 2013
(No difference)

OCA at 19:40, 7 January 2013

2013-01-07T19:40:08Z

← Older revision		Revision as of 19:40, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2raw.png\|left\|200px]]		[[Image:2raw.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2raw", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2raw\| PDB=2raw \| SCENE= }}		{{STRUCTURE_2raw\| PDB=2raw \| SCENE= }}

	===Crystal structure of the Borealin-Survivin complex===		===Crystal structure of the Borealin-Survivin complex===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17881355}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17881355 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17881355}}		{{ABSTRACT_PUBMED_17881355}}

	==About this Structure==		==About this Structure==
	~~2RAW~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA].		[[2raw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17881355~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017881355</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hymowitz, S G.]]		[[Category: Hymowitz, S G.]]
	~~[[Category: Alternative splicing]]~~
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Bir]]		[[Category: Bir]]
Line 34:		Line 21:
	[[Category: Chromosomal passender complex]]		[[Category: Chromosomal passender complex]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
	~~[[Category: Cytoplasm]]~~
	[[Category: Dasrab]]		[[Category: Dasrab]]
	[[Category: Iap]]		[[Category: Iap]]
Line 41:		Line 27:
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]
	~~[[Category: Polymorphism]]~~
	[[Category: Protease inhibitor]]		[[Category: Protease inhibitor]]
	[[Category: Thiol protease inhibitor]]		[[Category: Thiol protease inhibitor]]
	~~[[Category: Zinc]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:31:36 2009''~~

OCA at 09:31, 17 February 2009

2009-02-17T09:31:38Z

← Older revision		Revision as of 09:31, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2RAW is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA].		2RAW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAW OCA].

	==Reference==		==Reference==
	~~The mitotic regulator Survivin binds as a monomer to its functional interactor Borealin., Bourhis E, Hymowitz SG, Cochran AG, J Biol Chem. 2007 Nov 30;282(48):35018-23. Epub 2007 Sep 19.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17881355 17881355]~~		<ref group="xtra">PMID:17881355</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	~~[[Category: Protein complex]]~~
	[[Category: Hymowitz, S G.]]		[[Category: Hymowitz, S G.]]
	[[Category: Alternative splicing]]		[[Category: Alternative splicing]]
Line 47:		Line 46:
	[[Category: Zinc]]		[[Category: Zinc]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 15~~:37:~~52 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:31:36 2009''

OCA at 12:37, 28 July 2008

2008-07-28T12:37:55Z

← Older revision		Revision as of 12:37, 28 July 2008
Line 1:		Line 1:
	[[Image:2raw.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:2raw.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2raw\| PDB=2raw \| SCENE= }}		{{STRUCTURE_2raw\| PDB=2raw \| SCENE= }}

	~~'''~~Crystal structure of the Borealin-Survivin complex~~'''~~		===Crystal structure of the Borealin-Survivin complex===


	~~==Overview==~~		<!--
	Survivin is a member of the IAP (inhibitor of apoptosis) protein family, defined in part by the presence of a zinc-binding baculoviral inhibitory repeat (BIR) domain. Most BIR domains bind short sequences beginning with alanine, ~~and in this manner~~, ~~they recognize and block~~ the ~~action of key targets in apoptotic pathways. However, Survivin binds only very weakly~~ to ~~typical IAP ligands. Unique features of Survivin are~~ the ~~long C-terminal helix following the BIR domain and a short segment~~ (~~linking the helix and BIR domains~~) ~~that mediates Survivin homodimerization. Despite this detailed knowledge of the structure of Survivin itself~~, ~~there~~ is a current lack of understanding about how Survivin recognizes cellular binding partners, and consequently, many questions about Survivin function remain unanswered. We determined two co-crystal structures of Survivin and a minimal binding fragment from the ~~chromosomal passenger protein Borealin, a well validated functional interactor~~. ~~The interaction between Survivin and Borealin involves extensive packing between the long C~~-terminal helix of Survivin and a long Borealin helix. Surprisingly, an additional important interaction occurs between the Survivin homodimerization interface and a short segment of Borealin. This segment both structurally mimics and displaces one Survivin monomer. The relevance of this unexpected interaction was tested by mutagenesis of two key Borealin residues. Mutant Borealin introduced into HeLa cells failed to localize properly during mitosis and also caused mislocalization of other chromosomal passenger proteins. This suggests that the mutant is dominant-~~negative and confirms the functional importance of the interaction surface identified in the crystal structures.~~		The line below this paragraph, {{ABSTRACT_PUBMED_17881355}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17881355 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17881355}}

	==About this Structure==		==About this Structure==
Line 42:		Line 46:
	[[Category: Thiol protease inhibitor]]		[[Category: Thiol protease inhibitor]]
	[[Category: Zinc]]		[[Category: Zinc]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 16~~:34:10 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:37:52 2008''