2rog - Revision history

OCA at 19:14, 29 May 2024

2024-05-29T19:14:59Z

← Older revision		Revision as of 19:14, 29 May 2024
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	==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==		==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==
	<StructureSection load='2rog' size='340' side='right'caption='[[2rog~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2rog' size='340' side='right'caption='[[2rog]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2roe\|2roe]]</div~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [https://pdbe.org/2rog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [https://www.ebi.ac.uk/pdbsum/2rog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [https://pdbe.org/2rog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [https://www.ebi.ac.uk/pdbsum/2rog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q5SHL2_THET8 Q5SHL2_THET8]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Guentert, P]]		[[Category: Thermus thermophilus]]
	[[Category: Hamatsu, J]]		[[Category: Guentert P]]
	[[Category: Ikeya, T]]		[[Category: Hamatsu J]]
	[[Category: Ito, Y]]		[[Category: Ikeya T]]
	[[Category: Koyama, H]]		[[Category: Ito Y]]
	[[Category: Mikawa, T]]		[[Category: Koyama H]]
	[[Category: Mishima, M]]		[[Category: Mikawa T]]
	[[Category: Sakakibara, D]]		[[Category: Mishima M]]
	[[Category: Sasaki, A]]		[[Category: Sakakibara D]]
	[[Category: Shirakawa, M]]		[[Category: Sasaki A]]
	[[Category: Smith~~, B O~~]]		[[Category: Shirakawa M]]
	[[Category: Waelchli, M]]		[[Category: Smith BO]]
	~~[[Category: Metal binding protein]]~~		[[Category: Waelchli M]]
	~~[[Category: Protein~~]]

OCA at 06:54, 16 March 2022

2022-03-16T06:54:29Z

← Older revision		Revision as of 06:54, 16 March 2022
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	<StructureSection load='2rog' size='340' side='right'caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rog' size='340' side='right'caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2roe\|2roe]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [~~http~~://pdbe.org/2rog PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rog PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [https://pdbe.org/2rog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [https://www.ebi.ac.uk/pdbsum/2rog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:14, 10 June 2020

2020-06-10T07:14:22Z

← Older revision		Revision as of 07:14, 10 June 2020
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	==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==		==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==
	<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rog' size='340' side='right'caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://pdbe.org/2rog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://pdbe.org/2rog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]		[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
			[[Category: Large Structures]]
	[[Category: Guentert, P]]		[[Category: Guentert, P]]
	[[Category: Hamatsu, J]]		[[Category: Hamatsu, J]]

OCA at 11:01, 5 September 2018

2018-09-05T11:01:59Z

← Older revision		Revision as of 11:01, 5 September 2018
Line 1:		Line 1:

	==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==		==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==
	<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://pdbe.org/2rog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://pdbe.org/2rog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rog ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ro/2rog_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ro/2rog_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:17, 8 February 2016

2016-02-08T18:17:13Z

← Older revision		Revision as of 18:17, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://pdbe.org/2rog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rog ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2rog" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermus thermophilus~~]]		[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
	[[Category: Guentert, P]]		[[Category: Guentert, P]]
	[[Category: Hamatsu, J]]		[[Category: Hamatsu, J]]

OCA at 15:18, 19 January 2015

2015-01-19T15:18:00Z

← Older revision		Revision as of 15:18, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Guentert, P.]]		[[Category: Guentert, P]]
	[[Category: Hamatsu, J.]]		[[Category: Hamatsu, J]]
	[[Category: Ikeya, T.]]		[[Category: Ikeya, T]]
	[[Category: Ito, Y.]]		[[Category: Ito, Y]]
	[[Category: Koyama, H.]]		[[Category: Koyama, H]]
	[[Category: Mikawa, T.]]		[[Category: Mikawa, T]]
	[[Category: Mishima, M.]]		[[Category: Mishima, M]]
	[[Category: Sakakibara, D.]]		[[Category: Sakakibara, D]]
	[[Category: Sasaki, A.]]		[[Category: Sasaki, A]]
	[[Category: Shirakawa, M.]]		[[Category: Shirakawa, M]]
	[[Category: Smith, B O.]]		[[Category: Smith, B O]]
	[[Category: Waelchli, M.]]		[[Category: Waelchli, M]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Protein]]		[[Category: Protein]]

OCA at 01:57, 1 October 2014

2014-10-01T01:57:47Z

← Older revision		Revision as of 01:57, 1 October 2014
Line 1:		Line 1:
	[[Image:~~2rog~~.~~png~~\|~~left~~\|~~200px~~]]		==Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells==
			<StructureSection load='2rog' size='340' side='right' caption='[[2rog]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROG FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roe\|2roe]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rog OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rog RCSB], [http://www.ebi.ac.uk/pdbsum/2rog PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ro/2rog_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Investigating proteins 'at work' in a living environment at atomic resolution is a major goal of molecular biology, which has not been achieved even though methods for the three-dimensional (3D) structure determination of purified proteins in single crystals or in solution are widely used. Recent developments in NMR hardware and methodology have enabled the measurement of high-resolution heteronuclear multi-dimensional NMR spectra of macromolecules in living cells (in-cell NMR). Various intracellular events such as conformational changes, dynamics and binding events have been investigated by this method. However, the low sensitivity and the short lifetime of the samples have so far prevented the acquisition of sufficient structural information to determine protein structures by in-cell NMR. Here we show the first, to our knowledge, 3D protein structure calculated exclusively on the basis of information obtained in living cells. The structure of the putative heavy-metal binding protein TTHA1718 from Thermus thermophilus HB8 overexpressed in Escherichia coli cells was solved by in-cell NMR. Rapid measurement of the 3D NMR spectra by nonlinear sampling of the indirectly acquired dimensions was used to overcome problems caused by the instability and low sensitivity of living E. coli samples. Almost all of the expected backbone NMR resonances and most of the side-chain NMR resonances were observed and assigned, enabling high quality (0.96 angstrom backbone root mean squared deviation) structures to be calculated that are very similar to the in vitro structure of TTHA1718 determined independently. The in-cell NMR approach can thus provide accurate high-resolution structures of proteins in living environments.

	~~{{STRUCTURE_2rog\| PDB=2rog \| SCENE= }}~~		Protein structure determination in living cells by in-cell NMR spectroscopy.,Sakakibara D, Sasaki A, Ikeya T, Hamatsu J, Hanashima T, Mishima M, Yoshimasu M, Hayashi N, Mikawa T, Walchli M, Smith BO, Shirakawa M, Guntert P, Ito Y Nature. 2009 Mar 5;458(7234):102-5. PMID:19262674<ref>PMID:19262674</ref>

	~~===Solution structure~~ of ~~Thermus thermophilus HB8 TTHA1718 protein in living E~~. ~~coli cells===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19262674}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2rog]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019262674<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Guentert, P.]]		[[Category: Guentert, P.]]

OCA: Protected "2rog" [edit=sysop:move=sysop]

2013-01-07T21:24:33Z

Protected "2rog" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:24, 7 January 2013
(No difference)

OCA at 21:24, 7 January 2013

2013-01-07T21:24:30Z

← Older revision		Revision as of 21:24, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2rog.png\|left\|200px]]		[[Image:2rog.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2rog", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2rog\| PDB=2rog \| SCENE= }}		{{STRUCTURE_2rog\| PDB=2rog \| SCENE= }}

	===Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells===		===Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19262674}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19262674 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19262674}}		{{ABSTRACT_PUBMED_19262674}}

	==About this Structure==		==About this Structure==
	~~2ROG~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA].		[[2rog]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19262674~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019262674</ref><references group="xtra"/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Guentert, P.]]		[[Category: Guentert, P.]]
Line 39:		Line 27:
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Protein]]		[[Category: Protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 8 20:10:38 2009''~~

OCA at 17:10, 8 April 2009

2009-04-08T17:10:39Z

← Older revision		Revision as of 17:10, 8 April 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
	[[Image:2rog.~~jpg~~\|left\|200px]]		[[Image:2rog.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells===		===Solution structure of Thermus thermophilus HB8 TTHA1718 protein in living E. coli cells===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_19262674}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 19262674 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_19262674}}

	==About this Structure==		==About this Structure==
	2ROG is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA].		2ROG is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROG OCA].

			==Reference==
			<ref group="xtra">PMID:19262674</ref><references group="xtra"/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Guentert, P.]]		[[Category: Guentert, P.]]
Line 31:		Line 40:
	[[Category: Protein]]		[[Category: Protein]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar~~ ~~4 14~~:30:48 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 8 20:10:38 2009''