2sfa - Revision history

OCA at 01:25, 21 November 2024

2024-11-21T01:25:11Z

← Older revision		Revision as of 01:25, 21 November 2024
Line 14:		Line 14:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/2sfa_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/2sfa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 05:39, 5 June 2024

2024-06-05T05:39:33Z

← Older revision		Revision as of 05:39, 5 June 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [https://pdbe.org/2sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [https://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [https://pdbe.org/2sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [https://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/SFAS2_STRFR SFAS2_STRFR]] Broad substrate specificity.		[https://www.uniprot.org/uniprot/SFAS2_STRFR SFAS2_STRFR] Broad substrate specificity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 2sfa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2sfa" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 37:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Streptomyces fradiae]]		[[Category: Streptomyces fradiae]]
	[[Category: Kitadokoro, K]]		[[Category: Kitadokoro K]]
	[[Category: Tsuzuki, H]]		[[Category: Tsuzuki H]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Serine protease~~]]

OCA at 12:27, 23 March 2022

2022-03-23T12:27:35Z

← Older revision		Revision as of 12:27, 23 March 2022
Line 3:		Line 3:
	<StructureSection load='2sfa' size='340' side='right'caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2sfa' size='340' side='right'caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [~~http~~://pdbe.org/2sfa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [https://pdbe.org/2sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [https://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/SFAS2_STRFR SFAS2_STRFR]] Broad substrate specificity.		[[https://www.uniprot.org/uniprot/SFAS2_STRFR SFAS2_STRFR]] Broad substrate specificity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:06, 17 June 2020

2020-06-17T11:06:50Z

← Older revision		Revision as of 11:06, 17 June 2020
Line 1:		Line 1:

	==SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544==		==SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544==
	<StructureSection load='2sfa' size='340' side='right' caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2sfa' size='340' side='right'caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://pdbe.org/2sfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://pdbe.org/2sfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Streptomyces fradiae]]		[[Category: Streptomyces fradiae]]
	[[Category: Kitadokoro, K]]		[[Category: Kitadokoro, K]]

OCA at 08:27, 12 September 2018

2018-09-12T08:27:30Z

← Older revision		Revision as of 08:27, 12 September 2018
Line 1:		Line 1:

	==SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544==		==SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544==
	<StructureSection load='2sfa' size='340' side='right' caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2sfa' size='340' side='right' caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://pdbe.org/2sfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://pdbe.org/2sfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2sfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/2sfa_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/2sfa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:55, 9 February 2016

2016-02-09T14:55:57Z

← Older revision		Revision as of 14:55, 9 February 2016
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://pdbe.org/2sfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2sfa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2sfa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:32, 25 December 2014

2014-12-25T07:32:00Z

← Older revision		Revision as of 07:32, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/SFAS2_STRFR SFAS2_STRFR]] Broad substrate specificity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces fradiae]]		[[Category: Streptomyces fradiae]]
	[[Category: Kitadokoro, K.]]		[[Category: Kitadokoro, K]]
	[[Category: Tsuzuki, H.]]		[[Category: Tsuzuki, H]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 01:59, 1 October 2014

2014-10-01T01:59:20Z

← Older revision		Revision as of 01:59, 1 October 2014
Line 1:		Line 1:
	[[Image:~~2sfa~~.~~png~~\|~~left~~\|~~200px~~]]		==SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544==
			<StructureSection load='2sfa' size='340' side='right' caption='[[2sfa]], [[Resolution\|resolution]] 1.60Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2SFA FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2sfa RCSB], [http://www.ebi.ac.uk/pdbsum/2sfa PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/2sfa_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			A proteinase having wide substrate specificity was isolated from Streptomyces fradiae ATCC 14544. This proteinase, which we propose to call SFase-2, was purified from the culture filtrate by S-Sepharose chromatography. The purified enzyme showed an apparent molecular mass of 19 kDa on SDS/PAGE. When synthetic peptides were used as substrates, SFase-2 showed broad substrate specificity. It also hydrolyzed keratin, elastin and collagen as proteinaceous substrates. It was completely inhibited by diisopropylfluorophosphate and chymostatin, but not by tosylphenylalaninechloromethane, tosyllysinechloromethane or EDTA, indicating that it can be classified as a serine proteinase. The matured protein sequence of SFase-2 was determined by a combination of amino acid sequencing and the DNA sequencing of the gene. SFase-2, consisting of 191 amino acids, is a novel proteinase. It showed 76% similarity in the amino acid sequence with Streptomyces griseus proteinase A [Johnson P. and Smillie L. B. (1974) FEBS Lett. 47, 1-6]. For insight into the three-dimensional structure of SFase-2, we obtained single crystals by the vapor diffusion method using sodium phosphate as a precipitant. These crystals belonged to the orthorhombic, space group P2(1)2(1)2(1) with cell dimensions a = 6.92 nm, b = 7.28 nm, c = 2.99 nm; one molecule was present in the asymmetric unit.

	~~{{STRUCTURE_2sfa\| PDB=2sfa \| SCENE= }}~~		Purification, characterization, primary structure, crystallization and preliminary crystallographic study of a serine proteinase from Streptomyces fradiae ATCC 14544.,Kitadokoro K, Tsuzuki H, Nakamura E, Sato T, Teraoka H Eur J Biochem. 1994 Feb 15;220(1):55-61. PMID:8119298<ref>PMID:8119298</ref>

	~~===SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_8119298}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2sfa]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_fradiae Streptomyces fradiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:008119298<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Streptomyces fradiae]]		[[Category: Streptomyces fradiae]]
	[[Category: Kitadokoro, K.]]		[[Category: Kitadokoro, K.]]

OCA: Protected "2sfa" [edit=sysop:move=sysop]

2013-01-07T18:43:04Z

Protected "2sfa" [edit=sysop:move=sysop]

← Older revision	Revision as of 18:43, 7 January 2013
(No difference)

OCA at 18:43, 7 January 2013

2013-01-07T18:43:01Z

← Older revision		Revision as of 18:43, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2sfa.png\|left\|200px]]		[[Image:2sfa.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2sfa", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2sfa\| PDB=2sfa \| SCENE= }}		{{STRUCTURE_2sfa\| PDB=2sfa \| SCENE= }}

	===SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544===		===SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_8119298}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 8119298 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_8119298}}		{{ABSTRACT_PUBMED_8119298}}

	==About this Structure==		==About this Structure==
	~~2SFA~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA].		[[2sfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SFA OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~8119298~~</ref><references group="xtra"/>		<ref group="xtra">PMID:008119298</ref><references group="xtra"/>
	[[Category: Streptomyces fradiae]]		[[Category: Streptomyces fradiae]]
	[[Category: Kitadokoro, K.]]		[[Category: Kitadokoro, K.]]
Line 29:		Line 17:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:38:44 2009''~~