2v2d - Revision history

OCA at 15:02, 13 December 2023

2023-12-13T15:02:55Z

← Older revision		Revision as of 15:02, 13 December 2023
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	<StructureSection load='2v2d' size='340' side='right'caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v2d' size='340' side='right'caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Trybb Trybb~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [https://pdbe.org/2v2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [https://www.ebi.ac.uk/pdbsum/2v2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [https://pdbe.org/2v2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [https://www.ebi.ac.uk/pdbsum/2v2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2d ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Triose-phosphate isomerase]]~~		[[Category: Trypanosoma brucei brucei]]
	~~[[Category: Trybb~~]]		[[Category: Alahuhta M]]
	[[Category: Alahuhta, M]]		[[Category: Casteleijn MG]]
	[[Category: Casteleijn~~, M G~~]]		[[Category: Neubauer P]]
	[[Category: Neubauer, P]]		[[Category: Wierenga RK]]
	[[Category: Wierenga~~, R K]]~~
	~~[[Category: A178l]]~~
	~~[[Category: Engineering]]~~
	~~[[Category: Enzyme]]~~
	~~[[Category: Fatty acid biosynthesis]]~~
	~~[[Category: Gluconeogenesis]]~~
	~~[[Category: Glycolysis]]~~
	~~[[Category: Glycosome]]~~
	~~[[Category: Hinge]]~~
	~~[[Category: Isomerase]]~~
	~~[[Category: Lipid synthesis]]~~
	~~[[Category: Loop-6]]~~
	~~[[Category: Loop6]]~~
	~~[[Category: Monomeric]]~~
	~~[[Category: Pentose shunt]]~~
	~~[[Category: Point mutation]]~~
	~~[[Category: Tim]]~~
	~~[[Category: Tim-barrel]]~~
	~~[[Category: Triosephosphate isomerase~~]]

OCA at 10:41, 7 July 2021

2021-07-07T10:41:12Z

← Older revision		Revision as of 10:41, 7 July 2021
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	<StructureSection load='2v2d' size='340' side='right'caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v2d' size='340' side='right'caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2V2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [~~http~~://pdbe.org/2v2d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2v2d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [https://pdbe.org/2v2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [https://www.ebi.ac.uk/pdbsum/2v2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2d ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Triose ~~Phosphate Isomerase~~\|Triose ~~Phosphate Isomerase~~]]		*[[Triose phosphate isomerase 3D structures\|Triose phosphate isomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:37, 24 July 2019

2019-07-24T07:37:34Z

← Older revision		Revision as of 07:37, 24 July 2019
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	==The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM==		==The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM==
	<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v2d' size='340' side='right'caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Trybb]]		[[Category: Trybb]]

OCA at 08:43, 12 September 2018

2018-09-12T08:43:18Z

← Older revision		Revision as of 08:43, 12 September 2018
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	==~~THE~~ A178L ~~MUTATION IN THE~~ C-~~TERMINAL HINGE OF THE FLEXIBLE LOOP~~-6 ~~OF TRIOSEPHOSPHATE ISOMERASE~~ (TIM) ~~INDUCES A MORE CLOSED CONFORMATION OF THIS HINGE REGION IN DIMERIC AND MONOMERIC~~ TIM==
			==The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM==
	<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://pdbe.org/2v2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://pdbe.org/2v2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2d ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/2v2d_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/2v2d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:11, 8 February 2016

2016-02-08T15:11:36Z

← Older revision		Revision as of 15:11, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trypanosoma_brucei_brucei Trypanosoma brucei brucei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://pdbe.org/2v2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v2d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2v2d" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: ~~Trypanosoma brucei brucei~~]]		[[Category: Trybb]]
	[[Category: Alahuhta, M]]		[[Category: Alahuhta, M]]
	[[Category: Casteleijn, M G]]		[[Category: Casteleijn, M G]]

OCA at 18:15, 19 January 2015

2015-01-19T18:15:01Z

← Older revision		Revision as of 18:15, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Trypanosoma brucei brucei]]		[[Category: Trypanosoma brucei brucei]]
	[[Category: Alahuhta, M.]]		[[Category: Alahuhta, M]]
	[[Category: Casteleijn, M G.]]		[[Category: Casteleijn, M G]]
	[[Category: Neubauer, P.]]		[[Category: Neubauer, P]]
	[[Category: Wierenga, R K.]]		[[Category: Wierenga, R K]]
	[[Category: A178l]]		[[Category: A178l]]
	[[Category: Engineering]]		[[Category: Engineering]]

OCA at 02:08, 1 October 2014

2014-10-01T02:08:32Z

← Older revision		Revision as of 02:08, 1 October 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2v2d\| PDB=2v2d \| SCENE= }}~~		==THE A178L MUTATION IN THE C-TERMINAL HINGE OF THE FLEXIBLE LOOP-6 OF TRIOSEPHOSPHATE ISOMERASE (TIM) INDUCES A MORE CLOSED CONFORMATION OF THIS HINGE REGION IN DIMERIC AND MONOMERIC TIM==
	===THE A178L MUTATION IN THE C-TERMINAL HINGE OF THE FLEXIBLE LOOP-6 OF TRIOSEPHOSPHATE ISOMERASE (TIM) INDUCES A MORE CLOSED CONFORMATION OF THIS HINGE REGION IN DIMERIC AND MONOMERIC TIM===		<StructureSection load='2v2d' size='340' side='right' caption='[[2v2d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_18219118}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2D FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag1\|1ag1]], [[1dkw\|1dkw]], [[1iig\|1iig]], [[1iih\|1iih]], [[1kv5\|1kv5]], [[1ml1\|1ml1]], [[1mss\|1mss]], [[1mtm\|1mtm]], [[1tpd\|1tpd]], [[1tpe\|1tpe]], [[1tpf\|1tpf]], [[1trd\|1trd]], [[1tri\|1tri]], [[1tsi\|1tsi]], [[1tti\|1tti]], [[1ttj\|1ttj]], [[2j24\|2j24]], [[2j27\|2j27]], [[2v0t\|2v0t]], [[2v2c\|2v2c]], [[3tim\|3tim]], [[4tim\|4tim]], [[5tim\|5tim]], [[6tim\|6tim]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v2d RCSB], [http://www.ebi.ac.uk/pdbsum/2v2d PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/2v2d_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The flexible catalytic loop, loop-6, of TIM has evolved to preferably be open in the unliganded state and to preferably be closed in the liganded state. The N-terminal and C-terminal hinges of this loop are important for its opening/closing mechanism. In this study, a small conserved C-terminal hinge residue, Ala178, has been mutated into a residue with a larger side chain, Leu178. This mutation has been made in the dimeric trypanosomal wild-type TIM (wtTIM) and in its mutated catalytically competent monomeric variant (ml1TIM). The variants are referred to as A178L and ml1A178L, respectively. Crystal structures have been determined of unliganded A178L (at 2.2 A), liganded A178L (at 1.89 A), unliganded ml1A178L (at 2.3 A) and liganded ml1A178L (at 1.18 A) using the transition-state analogue 2-phosphoglycolate as a ligand. Structural characterization of the two variants shows that this mutation favours the closed conformation of the C-terminal hinge region, even in the absence of ligand. In the structure of the unliganded A178L variant a range of new loop-6 conformations are observed, including subunits in which the tip of loop-6 is completely disordered. The catalytic efficiency of A178L is lower than that of wtTIM, which correlates with the structural differences between the apo forms of wtTIM and A178L, in particular the more disordered loop-6 in the structure of unliganded A178L. In the liganded structures of A178L and ml1A178L the structural differences induced by the mutation are minimal. Structural characterization of the ml1A178L variant highlights its structural plasticity.

	~~==About this Structure==~~		Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM.,Alahuhta M, Casteleijn MG, Neubauer P, Wierenga RK Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):178-88. Epub 2008, Jan 16. PMID:18219118<ref>PMID:18219118</ref>
	~~[[2v2d]] is~~ a ~~1 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2V2D OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:018219118<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Trypanosoma brucei brucei]]		[[Category: Trypanosoma brucei brucei]]

OCA at 11:04, 11 March 2013

2013-03-11T11:04:21Z

← Older revision		Revision as of 11:04, 11 March 2013
Line 1:		Line 1:
	~~[[Image:2v2d.png\|left\|200px]]~~

	{{STRUCTURE_2v2d\| PDB=2v2d \| SCENE= }}		{{STRUCTURE_2v2d\| PDB=2v2d \| SCENE= }}

	===THE A178L MUTATION IN THE C-TERMINAL HINGE OF THE FLEXIBLE LOOP-6 OF TRIOSEPHOSPHATE ISOMERASE (TIM) INDUCES A MORE CLOSED CONFORMATION OF THIS HINGE REGION IN DIMERIC AND MONOMERIC TIM===		===THE A178L MUTATION IN THE C-TERMINAL HINGE OF THE FLEXIBLE LOOP-6 OF TRIOSEPHOSPHATE ISOMERASE (TIM) INDUCES A MORE CLOSED CONFORMATION OF THIS HINGE REGION IN DIMERIC AND MONOMERIC TIM===

	{{ABSTRACT_PUBMED_18219118}}		{{ABSTRACT_PUBMED_18219118}}

OCA at 10:34, 20 October 2012

2012-10-20T10:34:10Z

← Older revision		Revision as of 10:34, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2v2d]] is a 1 chain structure ~~of [[Triose Phosphate Isomerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA].		[[2v2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA].

	==See Also==		==See Also==

OCA at 02:45, 29 August 2012

2012-08-29T02:45:31Z

← Older revision		Revision as of 02:45, 29 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2v2d]] is a 1 chain structure of ~~[[TIM test]] and~~ [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA].		[[2v2d]] is a 1 chain structure of [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2D OCA].

	==See Also==		==See Also==
	*[[TIM test\|TIM test]]
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]