2vae - Revision history

OCA at 01:26, 21 November 2024

2024-11-21T01:26:30Z

← Older revision		Revision as of 01:26, 21 November 2024
Line 15:		Line 15:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vae_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vae_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 08:44, 15 November 2023

2023-11-15T08:44:55Z

← Older revision		Revision as of 08:44, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='2vae' size='340' side='right'caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='2vae' size='340' side='right'caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dissp Dissp~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[2vad\|2vad]]~~</~~div~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [https://pdbe.org/2vae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [https://www.ebi.ac.uk/pdbsum/2vae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vae ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [https://pdbe.org/2vae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [https://www.ebi.ac.uk/pdbsum/2vae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vae ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RFP_DISSP RFP_DISSP] Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.<ref>PMID:10504696</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Dissp~~]]		[[Category: Discosoma sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bevis, B]]		[[Category: Bevis B]]
	[[Category: Downing~~, M E~~]]		[[Category: Downing ME]]
	[[Category: Glick~~, B S~~]]		[[Category: Glick BS]]
	[[Category: Keenan~~, R J~~]]		[[Category: Keenan RJ]]
	[[Category: Khuong, N]]		[[Category: Khuong N]]
	[[Category: Strack~~, R L~~]]		[[Category: Strack RL]]
	[[Category: Strongin~~, D E~~]]		[[Category: Strongin DE]]
	[[Category: Sundaram, K]]		[[Category: Sundaram K]]
	~~[[Category: Chromophore]]~~
	~~[[Category: Dsred]]~~
	~~[[Category: Fast maturing]]~~
	~~[[Category: Fluorescent protein]]~~
	~~[[Category: Gfp-like]]~~
	~~[[Category: Luminescence]]~~
	~~[[Category: Photoprotein~~]]

OCA at 10:43, 7 July 2021

2021-07-07T10:43:32Z

← Older revision		Revision as of 10:43, 7 July 2021
Line 3:		Line 3:
	<StructureSection load='2vae' size='340' side='right'caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='2vae' size='340' side='right'caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vad\|2vad]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [~~http~~://pdbe.org/2vae PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vae PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vae ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [https://pdbe.org/2vae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [https://www.ebi.ac.uk/pdbsum/2vae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vae ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:10, 10 May 2019

2019-05-10T12:10:35Z

← Older revision		Revision as of 12:10, 10 May 2019
Line 1:		Line 1:

	==Fast maturing red fluorescent protein, DsRed.T4==		==Fast maturing red fluorescent protein, DsRed.T4==
	<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='2vae' size='340' side='right'caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Dissp]]		[[Category: Dissp]]
			[[Category: Large Structures]]
	[[Category: Bevis, B]]		[[Category: Bevis, B]]
	[[Category: Downing, M E]]		[[Category: Downing, M E]]

OCA at 08:57, 12 September 2018

2018-09-12T08:57:19Z

← Older revision		Revision as of 08:57, 12 September 2018
Line 1:		Line 1:
	==~~FAST MATURING RED FLUORESCENT PROTEIN~~, ~~DSRED~~.T4==
			==Fast maturing red fluorescent protein, DsRed.T4==
	<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://pdbe.org/2vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://pdbe.org/2vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vae ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vae_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vae_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:23, 9 February 2016

2016-02-09T10:23:58Z

← Older revision		Revision as of 10:23, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Discosoma_sp. Discosoma sp.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dissp Dissp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://pdbe.org/2vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vae ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2vae" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Discosoma sp~~]]		[[Category: Dissp]]
	[[Category: Bevis, B]]		[[Category: Bevis, B]]
	[[Category: Downing, M E]]		[[Category: Downing, M E]]

OCA at 17:07, 19 January 2015

2015-01-19T17:07:58Z

← Older revision		Revision as of 17:07, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Discosoma sp.]]		[[Category: Discosoma sp]]
	[[Category: Bevis, B.]]		[[Category: Bevis, B]]
	[[Category: Downing, M E.]]		[[Category: Downing, M E]]
	[[Category: Glick, B S.]]		[[Category: Glick, B S]]
	[[Category: Keenan, R J.]]		[[Category: Keenan, R J]]
	[[Category: Khuong, N.]]		[[Category: Khuong, N]]
	[[Category: Strack, R L.]]		[[Category: Strack, R L]]
	[[Category: Strongin, D E.]]		[[Category: Strongin, D E]]
	[[Category: Sundaram, K.]]		[[Category: Sundaram, K]]
	[[Category: Chromophore]]		[[Category: Chromophore]]
	[[Category: Dsred]]		[[Category: Dsred]]

OCA at 05:37, 3 October 2014

2014-10-03T05:37:26Z

← Older revision		Revision as of 05:37, 3 October 2014
Line 1:		Line 1:
	[[Image:~~2vae~~.~~png~~\|~~left~~\|~~200px~~]]		==FAST MATURING RED FLUORESCENT PROTEIN, DSRED.T4==
			<StructureSection load='2vae' size='340' side='right' caption='[[2vae]], [[Resolution\|resolution]] 1.64Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VAE FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vad\|2vad]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vae RCSB], [http://www.ebi.ac.uk/pdbsum/2vae PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vae_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The red fluorescent protein DsRed has been extensively engineered for use as an in vivo research tool. In fast maturing DsRed variants, the chromophore maturation half-time is approximately 40 min, compared to approximately 12 h for wild-type DsRed. Further, DsRed has been converted from a tetramer into a monomer, a task that entailed mutating approximately 20% of the amino acids. These engineered variants of DsRed have proven extremely valuable for biomedical research, but the structural basis for the improved characteristics has not been thoroughly investigated. Here we present a 1.7 A crystal structure of the fast maturing tetrameric variant DsRed.T4. We also present a biochemical characterization and 1.6 A crystal structure of the monomeric variant DsRed.M1, also known as DsRed-Monomer. Analysis of the crystal structures suggests that rearrangements of Ser69 and Glu215 contribute to fast maturation, and that positioning of the Lys70 side chain modulates fluorescence quantum yield. Despite the 45 mutations in DsRed.M1 relative to wild-type DsRed, there is a root-mean-square deviation of only 0.3 A between the two structures. We propose that novel intramolecular interactions in DsRed.M1 partially compensate for the loss of intermolecular interactions found in the tetramer.

	~~{{STRUCTURE_2vae\| PDB=2vae \| SCENE= }}~~		Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.,Strongin DE, Bevis B, Khuong N, Downing ME, Strack RL, Sundaram K, Glick BS, Keenan RJ Protein Eng Des Sel. 2007 Nov;20(11):525-34. Epub 2007 Oct 25. PMID:17962222<ref>PMID:17962222</ref>

	~~===FAST MATURING RED FLUORESCENT PROTEIN~~, ~~DSRED.T4===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17962222}}~~

	~~==About this Structure==~~
	~~[[2vae]] is~~ a ~~8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Discosoma_sp~~. ~~Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2VAE OCA].~~

	==See Also==		==See Also==
	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
	*[[Sandbox104\|Sandbox104]]		== References ==
	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:017962222<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Discosoma sp.]]		[[Category: Discosoma sp.]]
	[[Category: Bevis, B.]]		[[Category: Bevis, B.]]

OCA at 13:33, 20 October 2012

2012-10-20T13:33:55Z

← Older revision		Revision as of 13:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2vae]] is a 8 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA].		[[2vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAE OCA].

	==See Also==		==See Also==

OCA: Protected "2vae" [edit=sysop:move=sysop]

2012-07-26T12:23:06Z

Protected "2vae" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:23, 26 July 2012
(No difference)