2vtk - Revision history

OCA at 09:24, 21 February 2024

2024-02-21T09:24:46Z

← Older revision		Revision as of 09:24, 21 February 2024
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	<StructureSection load='2vtk' size='340' side='right'caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2vtk' size='340' side='right'caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Hhv-1 Hhv-~~1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VTK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_F Human alphaherpesvirus 1 strain F]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE-5~~-~~DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=THM:THYMIDINE'>THM</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [https://pdbe.org/2vtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [https://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [https://pdbe.org/2vtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [https://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/KITH_HHV11 KITH_HHV11]] In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome (By similarity).		[https://www.uniprot.org/uniprot/KITH_HHV11 KITH_HHV11] In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vtk ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vtk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Thymidine kinase from Herpes simplex virus type 1 (TK) was crystallized in an N-terminally truncated but fully active form. The structures of TK complexed with ADP at the ATP-site and deoxythymidine-5'-monophosphate (dTMP), deoxythymidine (dT), or idoxuridine-5'-phosphate (5-iodo-dUMP) at the substrate-site were refined to 2.75 A, 2.8 A, and 3.0 A resolution, respectively. TK catalyzes the phosphorylation of dT resulting in an ester, and the phosphorylation of dTMP giving rise to an anhydride. The presented TK structures indicate that there are only small differences between these two modes of action. Glu83 serves as a general base in the ester reaction. Arg163 parks at an internal aspartate during ester formation and binds the alpha-phosphate of dTMP during anhydride formation. The bound deoxythymidine leaves a 35 A3 cavity at position 5 of the base and two sequestered water molecules at position 2. Cavity and water molecules reduce the substrate specificity to such an extent that TK can phosphorylate various substrate analogues useful in pharmaceutical applications. TK is structurally homologous to the well-known nucleoside monophosphate kinases but contains large additional peptide segments.

	The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue.,Wild K, Bohner T, Folkers G, Schulz GE Protein Sci. 1997 Oct;6(10):2097-106. PMID:9336833<ref>PMID:9336833</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2vtk" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Thymidine kinase\|Thymidine kinase]]		*[[Thymidine kinase 3D structures\|Thymidine kinase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Hhv-~~1]]		[[Category: Human alphaherpesvirus 1 strain F]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Thymidine kinase]]~~		[[Category: Schulz GE]]
	[[Category: Schulz~~, G E~~]]		[[Category: Wild K]]
	[[Category: Wild, K]]
	~~[[Category: Additional thymidylate kinase activity]]~~
	~~[[Category: Key enzyme in thymidine salvage pathway]]~~
	~~[[Category: Target for anti-herpes viral drug]]~~
	~~[[Category: Transferase~~]]

OCA at 11:46, 30 March 2022

2022-03-30T11:46:07Z

← Older revision		Revision as of 11:46, 30 March 2022
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	<StructureSection load='2vtk' size='340' side='right'caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2vtk' size='340' side='right'caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2VTK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [~~http~~://pdbe.org/2vtk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [https://pdbe.org/2vtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [https://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/KITH_HHV11 KITH_HHV11]] In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome (By similarity).		[[https://www.uniprot.org/uniprot/KITH_HHV11 KITH_HHV11]] In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:30, 25 June 2020

2020-06-25T08:30:31Z

← Older revision		Revision as of 08:30, 25 June 2020
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	==THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE==		==THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE==
	<StructureSection load='2vtk' size='340' side='right' caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2vtk' size='340' side='right'caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VTK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://pdbe.org/2vtk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://pdbe.org/2vtk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Hhv-1]]		[[Category: Hhv-1]]
			[[Category: Large Structures]]
	[[Category: Thymidine kinase]]		[[Category: Thymidine kinase]]
	[[Category: Schulz, G E]]		[[Category: Schulz, G E]]

OCA at 21:25, 19 September 2018

2018-09-19T21:25:06Z

← Older revision		Revision as of 21:25, 19 September 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vt/2vtk_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vt/2vtk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:26, 5 August 2016

2016-08-05T06:26:00Z

← Older revision		Revision as of 06:26, 5 August 2016
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	==THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE==		==THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE==
	<StructureSection load='2vtk' size='340' side='right' caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2vtk' size='340' side='right' caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Viruses Viruses~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VTK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://pdbe.org/2vtk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vtk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vtk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2vtk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Hhv-1]]
	[[Category: Thymidine kinase]]		[[Category: Thymidine kinase]]
	~~[[Category: Viruses]]~~
	[[Category: Schulz, G E]]		[[Category: Schulz, G E]]
	[[Category: Wild, K]]		[[Category: Wild, K]]

OCA at 16:30, 25 December 2014

2014-12-25T16:30:25Z

← Older revision		Revision as of 16:30, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VTK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/KITH_HHV11 KITH_HHV11]] In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Thymidine kinase]]		[[Category: Thymidine kinase]]
	[[Category: Viruses]]		[[Category: Viruses]]
	[[Category: Schulz, G E.]]		[[Category: Schulz, G E]]
	[[Category: Wild, K.]]		[[Category: Wild, K]]
	[[Category: Additional thymidylate kinase activity]]		[[Category: Additional thymidylate kinase activity]]
	[[Category: Key enzyme in thymidine salvage pathway]]		[[Category: Key enzyme in thymidine salvage pathway]]
	[[Category: Target for anti-herpes viral drug]]		[[Category: Target for anti-herpes viral drug]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:20, 29 September 2014

2014-09-29T09:20:23Z

← Older revision		Revision as of 09:20, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2vtk~~.~~png~~\|~~left~~\|~~200px~~]]		==THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE==
			<StructureSection load='2vtk' size='340' side='right' caption='[[2vtk]], [[Resolution\|resolution]] 2.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VTK FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene><br>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vtk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vtk RCSB], [http://www.ebi.ac.uk/pdbsum/2vtk PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vt/2vtk_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Thymidine kinase from Herpes simplex virus type 1 (TK) was crystallized in an N-terminally truncated but fully active form. The structures of TK complexed with ADP at the ATP-site and deoxythymidine-5'-monophosphate (dTMP), deoxythymidine (dT), or idoxuridine-5'-phosphate (5-iodo-dUMP) at the substrate-site were refined to 2.75 A, 2.8 A, and 3.0 A resolution, respectively. TK catalyzes the phosphorylation of dT resulting in an ester, and the phosphorylation of dTMP giving rise to an anhydride. The presented TK structures indicate that there are only small differences between these two modes of action. Glu83 serves as a general base in the ester reaction. Arg163 parks at an internal aspartate during ester formation and binds the alpha-phosphate of dTMP during anhydride formation. The bound deoxythymidine leaves a 35 A3 cavity at position 5 of the base and two sequestered water molecules at position 2. Cavity and water molecules reduce the substrate specificity to such an extent that TK can phosphorylate various substrate analogues useful in pharmaceutical applications. TK is structurally homologous to the well-known nucleoside monophosphate kinases but contains large additional peptide segments.

	~~{{STRUCTURE_2vtk\| PDB=2vtk \| SCENE= }}~~		The structures of thymidine kinase from herpes simplex virus type 1 in complex with substrates and a substrate analogue.,Wild K, Bohner T, Folkers G, Schulz GE Protein Sci. 1997 Oct;6(10):2097-106. PMID:9336833<ref>PMID:9336833</ref>

	~~===THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9336833}}~~

	~~==About this Structure==~~
	~~[[2vtk]] is~~ a ~~1 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Viruses Viruses]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=2VTK OCA].~~

	==See Also==		==See Also==
	*[[Thymidine kinase\|Thymidine kinase]]		*[[Thymidine kinase\|Thymidine kinase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:009336833<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Thymidine kinase]]		[[Category: Thymidine kinase]]
	[[Category: Viruses]]		[[Category: Viruses]]

OCA at 18:55, 20 October 2012

2012-10-20T18:55:50Z

← Older revision		Revision as of 18:55, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2vtk]] is a 1 chain structure ~~of [[Thymidine kinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA].		[[2vtk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA].

	==See Also==		==See Also==

OCA: Protected "2vtk" [edit=sysop:move=sysop]

2012-07-27T05:43:41Z

Protected "2vtk" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:43, 27 July 2012
(No difference)

OCA at 05:43, 27 July 2012

2012-07-27T05:43:40Z

← Older revision		Revision as of 05:43, 27 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2vtk.png\|left\|200px]]		[[Image:2vtk.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2vtk", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2vtk\| PDB=2vtk \| SCENE= }}		{{STRUCTURE_2vtk\| PDB=2vtk \| SCENE= }}

	===THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE===		===THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE 1 IN COMPLEX WITH ADP AND DEOXYTHYMIDINE===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_9336833}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 9336833 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_9336833}}		{{ABSTRACT_PUBMED_9336833}}

	==About this Structure==		==About this Structure==
	~~2VTK~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Human_herpesvirus_1 Human herpesvirus 1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA].		[[2vtk]] is a 1 chain structure of [[Thymidine kinase]] with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VTK OCA].

			==See Also==
			*[[Thymidine kinase\|Thymidine kinase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9336833~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009336833</ref><references group="xtra"/>
	~~[[Category: Human herpesvirus 1]]~~
	[[Category: Thymidine kinase]]		[[Category: Thymidine kinase]]
			[[Category: Viruses]]
	[[Category: Schulz, G E.]]		[[Category: Schulz, G E.]]
	[[Category: Wild, K.]]		[[Category: Wild, K.]]
Line 32:		Line 23:
	[[Category: Target for anti-herpes viral drug]]		[[Category: Target for anti-herpes viral drug]]
	[[Category: Transferase]]		[[Category: Transferase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:49:09 2009''~~