2w2d - Revision history

OCA at 05:33, 17 October 2024

2024-10-17T05:33:30Z

← Older revision		Revision as of 05:33, 17 October 2024
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	<StructureSection load='2w2d' size='340' side='right'caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2w2d' size='340' side='right'caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.59Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [https://pdbe.org/2w2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [https://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [https://pdbe.org/2w2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [https://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.		[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2d_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~		[[Category: Clostridium botulinum]]
	~~[[Category: Bontoxilysin~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Acharya~~, K R~~]]		[[Category: Acharya KR]]
	[[Category: Chaddock~~, J A~~]]		[[Category: Chaddock JA]]
	[[Category: James~~, P L~~]]		[[Category: James PL]]
	[[Category: Marks~~, P M.H~~]]		[[Category: Marks PMH]]
	[[Category: Masuyer, G]]		[[Category: Masuyer G]]
	[[Category: Thiyagarajan, N]]		[[Category: Thiyagarajan N]]
	~~[[Category: Bont]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Membrane domain]]~~
	~~[[Category: Metal-binding]]~~
	~~[[Category: Metalloprotease]]~~
	~~[[Category: Mixed alpha and beta]]~~
	~~[[Category: Neurotoxin]]~~
	~~[[Category: Pharmaceutical]]~~
	~~[[Category: Protease]]~~
	~~[[Category: Protein engineering]]~~
	~~[[Category: Secreted]]~~
	~~[[Category: Transmembrane]]~~
	~~[[Category: Zinc protease~~]]

OCA at 11:51, 30 March 2022

2022-03-30T11:51:25Z

← Older revision		Revision as of 11:51, 30 March 2022
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	<StructureSection load='2w2d' size='340' side='right'caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2w2d' size='340' side='right'caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2W2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [~~http~~://pdbe.org/2w2d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [https://pdbe.org/2w2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [https://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.		[[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:50, 25 June 2020

2020-06-25T08:50:52Z

← Older revision		Revision as of 08:50, 25 June 2020
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	==Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A==		==Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A==
	<StructureSection load='2w2d' size='340' side='right' caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2w2d' size='340' side='right'caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2W2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://pdbe.org/2w2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://pdbe.org/2w2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]		*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
			[[Category: Large Structures]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
	[[Category: Chaddock, J A]]		[[Category: Chaddock, J A]]

OCA at 20:43, 19 September 2018

2018-09-19T20:43:38Z

← Older revision		Revision as of 20:43, 19 September 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2d_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 52:		Line 52:
	[[Category: Mixed alpha and beta]]		[[Category: Mixed alpha and beta]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
			[[Category: Pharmaceutical]]
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]

OCA at 05:35, 5 August 2016

2016-08-05T05:35:09Z

← Older revision		Revision as of 05:35, 5 August 2016
Line 1:		Line 1:

	==Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A==		==Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A==
	<StructureSection load='2w2d' size='340' side='right' caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2w2d' size='340' side='right' caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W2D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W2D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://pdbe.org/2w2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w2d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2w2d" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	~~[[Category: Clostridium botulinum]]~~
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
	[[Category: Chaddock, J A]]		[[Category: Chaddock, J A]]

OCA at 16:10, 25 December 2014

2014-12-25T16:10:17Z

← Older revision		Revision as of 16:10, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Acharya, K R.]]		[[Category: Acharya, K R]]
	[[Category: Chaddock, J A.]]		[[Category: Chaddock, J A]]
	[[Category: James, P L.]]		[[Category: James, P L]]
	[[Category: Marks, P M.H.]]		[[Category: Marks, P M.H]]
	[[Category: Masuyer, G.]]		[[Category: Masuyer, G]]
	[[Category: Thiyagarajan, N.]]		[[Category: Thiyagarajan, N]]
	[[Category: Bont]]		[[Category: Bont]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:56, 10 October 2014

2014-10-10T05:56:46Z

← Older revision		Revision as of 05:56, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2w2d' size='340' side='right' caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='2w2d' size='340' side='right' caption='[[2w2d]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. <br>		<table><tr><td colspan='2'>[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W2D FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.~~2 2~~.7.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w2d RCSB], [http://www.ebi.ac.uk/pdbsum/2w2d PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 23:
	Crystal structure of a catalytically active, non-toxic endopeptidase derivative of Clostridium botulinum toxin A.,Masuyer G, Thiyagarajan N, James PL, Marks PM, Chaddock JA, Acharya KR Biochem Biophys Res Commun. 2009 Mar 27;381(1):50-3. Epub 2009 Feb 8. PMID:19351593<ref>PMID:19351593</ref>		Crystal structure of a catalytically active, non-toxic endopeptidase derivative of Clostridium botulinum toxin A.,Masuyer G, Thiyagarajan N, James PL, Marks PM, Chaddock JA, Acharya KR Biochem Biophys Res Commun. 2009 Mar 27;381(1):50-3. Epub 2009 Feb 8. PMID:19351593<ref>PMID:19351593</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:38, 7 May 2014

2014-05-07T08:38:35Z

@@ Line 1: / Line 1: @@
-[[Image:2w2d.png|left|200px]]
+==Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A==
-<!--
+Crystal structure of a catalytically active, non-toxic endopeptidase derivative of Clostridium botulinum toxin A.,Masuyer G, Thiyagarajan N, James PL, Marks PM, Chaddock JA, Acharya KR Biochem Biophys Res Commun. 2009 Mar 27;381(1):50-3. Epub 2009 Feb 8. PMID:19351593<ref>PMID:19351593</ref>
-===Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_19351593}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 19351593 is the PubMed ID number.
+</StructureSection>
 [[Category: Bontoxilysin]]
 [[Category: Clostridium botulinum]]

OCA: Protected "2w2d" [edit=sysop:move=sysop]

2012-03-28T06:44:30Z

Protected "2w2d" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:44, 28 March 2012
(No difference)

OCA at 06:44, 28 March 2012

2012-03-28T06:44:28Z

← Older revision		Revision as of 06:44, 28 March 2012
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:2w2d.png\|left\|200px]]
	[[Image:2w2d.~~jpg~~\|left\|200px]]

	<!--		<!--
Line 10:		Line 9:
	{{STRUCTURE_2w2d\| PDB=2w2d \| SCENE= }}		{{STRUCTURE_2w2d\| PDB=2w2d \| SCENE= }}

	===~~CRYSTAL STRUCTURE OF A CATALYTICALLY ACTIVE~~, ~~NON~~-~~TOXIC ENDOPEPTIDASE DERIVATIVE OF CLOSTRIDIUM BOTULINUM TOXIN~~ A===		===Crystal Structure of a Catalytically Active, Non-toxic Endopeptidase Derivative of Clostridium botulinum Toxin A===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_19351593}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 19351593 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_19351593}}

	==About this Structure==		==About this Structure==
	~~2W2D~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA].		[[2w2d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2D OCA].

			==Reference==
			<ref group="xtra">PMID:019351593</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Acharya, K R.]]		[[Category: Acharya, K R.]]
	[[Category: Chaddock, J A.]]		[[Category: Chaddock, J A.]]
	[[Category: James, P.]]		[[Category: James, P L.]]
	[[Category: Marks, P.]]		[[Category: Marks, P M.H.]]
	[[Category: Masuyer, G.]]		[[Category: Masuyer, G.]]
	[[Category: Thiyagarajan, N.]]		[[Category: Thiyagarajan, N.]]
	[[Category: Bont]]		[[Category: Bont]]
	~~[[Category: Endopeptidase]]~~
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Membrane]]		[[Category: Membrane]]
Line 32:		Line 39:
	[[Category: Mixed alpha and beta]]		[[Category: Mixed alpha and beta]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	~~[[Category: Pharmaceutical]]~~
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
	[[Category: Secreted]]		[[Category: Secreted]]
	~~[[Category: Toxin]]~~
	[[Category: Transmembrane]]		[[Category: Transmembrane]]
	~~[[Category: Zinc]]~~
	[[Category: Zinc protease]]		[[Category: Zinc protease]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:10:26 2009''~~