2wsb - Revision history

OCA at 07:10, 1 May 2024

2024-05-01T07:10:30Z

← Older revision		Revision as of 07:10, 1 May 2024
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	<StructureSection load='2wsb' size='340' side='right'caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='2wsb' size='340' side='right'caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSB FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [https://pdbe.org/2wsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [https://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [https://pdbe.org/2wsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [https://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/GATDH_CERSP GATDH_CERSP] Catalyzes the interconversion of galactitol to the rare sugar L-tagatose (PubMed:7551050). Shows activity with a wide range of substrates, and catalyzes the oxidation of a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols (PubMed:7551050, PubMed:15296184). Shows high activity with D-threitol, xylitol, 1,2-hexanediol, 1,2-pentanediol, 2-hexanol, L-erythrulose, D-ribulose and acetoin (PubMed:7551050). Specific for NAD(+) (PubMed:7551050).<ref>PMID:15296184</ref> <ref>PMID:7551050</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wsb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wsb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Galactitol 2-dehydrogenase (GatDH) belongs to the protein superfamily of short-chain dehydrogenases. As an enzyme capable of the stereo- and regioselective modification of carbohydrates, it exhibits a high potential for application in biotechnology as a biocatalyst. We have determined the crystal structure of the binary form of GatDH in complex with its cofactor NAD(H) and of the ternary form in complex with NAD(H) and three different substrates. The active form of GatDH constitutes a homo-tetramer with two magnesium-ion binding sites each formed by two opposing C termini. The catalytic tetrad is formed by Asn(116), Ser(144), Tyr(159), and Lys(163). GatDH structurally aligns well with related members of the short-chain dehydrogenase family. The substrate binding pocket can be divided into two parts of different size and polarity. In the smaller part, the side chains of amino acids Ser(144), Ser(146), and Asn(151) are important determinants for the binding specificity and the orientation of (pro-) chiral compounds. The larger part of the pocket is elongated and flanked by polar and non-polar residues, enabling a rather broad substrate spectrum. The presented structures provide valuable information for a rational design of this enzyme to improve its stability against pH, temperature, or solvent concentration and to optimize product yield in bioreactors.

	Structural insight into substrate differentiation of the sugar-metabolizing enzyme galactitol dehydrogenase from Rhodobacter sphaeroides D.,Carius Y, Christian H, Faust A, Zander U, Klink BU, Kornberger P, Kohring GW, Giffhorn F, Scheidig AJ J Biol Chem. 2010 Jun 25;285(26):20006-14. Epub 2010 Apr 21. PMID:20410293<ref>PMID:20410293</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2wsb" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Rhodococcus capsulatus molisch 1907]]~~		[[Category: Cereibacter sphaeroides]]
	~~[[Category: Galactitol 2-dehydrogenase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Carius, Y]]		[[Category: Carius Y]]
	[[Category: Christian, H]]		[[Category: Christian H]]
	[[Category: Faust, A]]		[[Category: Faust A]]
	[[Category: Giffhorn, F]]		[[Category: Giffhorn F]]
	[[Category: Kohring~~, G W~~]]		[[Category: Kohring GW]]
	[[Category: Kornberger, P]]		[[Category: Kornberger P]]
	[[Category: Scheidig~~, A J]]~~		[[Category: Scheidig AJ]]
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Rossmann fold]]~~
	~~[[Category: Sdr]]~~
	~~[[Category: Tagatose~~]]

OCA at 11:15, 6 April 2022

2022-04-06T11:15:07Z

← Older revision		Revision as of 11:15, 6 April 2022
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	<StructureSection load='2wsb' size='340' side='right'caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='2wsb' size='340' side='right'caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WSB FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [~~http~~://pdbe.org/2wsb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [https://pdbe.org/2wsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [https://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:07, 1 July 2020

2020-07-01T09:07:57Z

← Older revision		Revision as of 09:07, 1 July 2020
Line 1:		Line 1:

	==Crystal structure of the short-chain dehydrogenase Galactitol- Dehydrogenase (GatDH) of Rhodobacter sphaeroides in complex with NAD==		==Crystal structure of the short-chain dehydrogenase Galactitol- Dehydrogenase (GatDH) of Rhodobacter sphaeroides in complex with NAD==
	<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='2wsb' size='340' side='right'caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WSB FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WSB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [http://pdbe.org/2wsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [http://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [http://pdbe.org/2wsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [http://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	[[Category: Rhodococcus capsulatus molisch 1907]]		[[Category: Rhodococcus capsulatus molisch 1907]]
	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
			[[Category: Large Structures]]
	[[Category: Carius, Y]]		[[Category: Carius, Y]]
	[[Category: Christian, H]]		[[Category: Christian, H]]

OCA at 09:21, 26 September 2018

2018-09-26T09:21:25Z

← Older revision		Revision as of 09:21, 26 September 2018
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	==~~CRYSTAL STRUCTURE OF THE SHORT~~-~~CHAIN DEHYDROGENASE GALACTITOL~~-~~DEHYDROGENASE~~ (~~GATDH~~) ~~OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH~~ NAD==		==Crystal structure of the short-chain dehydrogenase Galactitol- Dehydrogenase (GatDH) of Rhodobacter sphaeroides in complex with NAD==
	<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsb_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:57, 11 August 2016

2016-08-11T20:57:20Z

← Older revision		Revision as of 20:57, 11 August 2016
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD==		==CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD==
	<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodobacter_sphaeroides Rhodobacter sphaeroides~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WSB FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WSB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [http://www.ebi.ac.uk/pdbsum/2wsb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [http://pdbe.org/2wsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [http://www.ebi.ac.uk/pdbsum/2wsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wsb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2wsb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Rhodococcus capsulatus molisch 1907]]
	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
	~~[[Category: Rhodobacter sphaeroides]]~~
	[[Category: Carius, Y]]		[[Category: Carius, Y]]
	[[Category: Christian, H]]		[[Category: Christian, H]]

OCA at 06:26, 22 January 2015

2015-01-22T06:26:57Z

← Older revision		Revision as of 06:26, 22 January 2015
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	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
	[[Category: Rhodobacter sphaeroides]]		[[Category: Rhodobacter sphaeroides]]
	[[Category: Carius, Y.]]		[[Category: Carius, Y]]
	[[Category: Christian, H.]]		[[Category: Christian, H]]
	[[Category: Faust, A.]]		[[Category: Faust, A]]
	[[Category: Giffhorn, F.]]		[[Category: Giffhorn, F]]
	[[Category: Kohring, G W.]]		[[Category: Kohring, G W]]
	[[Category: Kornberger, P.]]		[[Category: Kornberger, P]]
	[[Category: Scheidig, A J.]]		[[Category: Scheidig, A J]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Sdr]]		[[Category: Sdr]]
	[[Category: Tagatose]]		[[Category: Tagatose]]

OCA at 11:16, 20 October 2014

2014-10-20T11:16:15Z

← Older revision		Revision as of 11:16, 20 October 2014
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	[[Image:~~2wsb~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD==
			<StructureSection load='2wsb' size='340' side='right' caption='[[2wsb]], [[Resolution\|resolution]] 1.25Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WSB FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=POL:N-PROPANOL'>POL</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol_2-dehydrogenase Galactitol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.16 1.1.1.16] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsb RCSB], [http://www.ebi.ac.uk/pdbsum/2wsb PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsb_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Galactitol 2-dehydrogenase (GatDH) belongs to the protein superfamily of short-chain dehydrogenases. As an enzyme capable of the stereo- and regioselective modification of carbohydrates, it exhibits a high potential for application in biotechnology as a biocatalyst. We have determined the crystal structure of the binary form of GatDH in complex with its cofactor NAD(H) and of the ternary form in complex with NAD(H) and three different substrates. The active form of GatDH constitutes a homo-tetramer with two magnesium-ion binding sites each formed by two opposing C termini. The catalytic tetrad is formed by Asn(116), Ser(144), Tyr(159), and Lys(163). GatDH structurally aligns well with related members of the short-chain dehydrogenase family. The substrate binding pocket can be divided into two parts of different size and polarity. In the smaller part, the side chains of amino acids Ser(144), Ser(146), and Asn(151) are important determinants for the binding specificity and the orientation of (pro-) chiral compounds. The larger part of the pocket is elongated and flanked by polar and non-polar residues, enabling a rather broad substrate spectrum. The presented structures provide valuable information for a rational design of this enzyme to improve its stability against pH, temperature, or solvent concentration and to optimize product yield in bioreactors.

	~~{{STRUCTURE_2wsb\| PDB=2wsb \| SCENE= }}~~		Structural insight into substrate differentiation of the sugar-metabolizing enzyme galactitol dehydrogenase from Rhodobacter sphaeroides D.,Carius Y, Christian H, Faust A, Zander U, Klink BU, Kornberger P, Kohring GW, Giffhorn F, Scheidig AJ J Biol Chem. 2010 Jun 25;285(26):20006-14. Epub 2010 Apr 21. PMID:20410293<ref>PMID:20410293</ref>

	~~===CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_20410293}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2wsb]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:020410293<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
	[[Category: Rhodobacter sphaeroides]]		[[Category: Rhodobacter sphaeroides]]

OCA: Protected "2wsb" [edit=sysop:move=sysop]

2013-01-07T23:00:20Z

Protected "2wsb" [edit=sysop:move=sysop]

← Older revision	Revision as of 23:00, 7 January 2013
(No difference)

OCA at 23:00, 7 January 2013

2013-01-07T23:00:15Z

← Older revision		Revision as of 23:00, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2wsb.png\|left\|200px]]		[[Image:2wsb.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2wsb", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2wsb\| PDB=2wsb \| SCENE= }}		{{STRUCTURE_2wsb\| PDB=2wsb \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD===		===CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20410293}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20410293 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20410293}}		{{ABSTRACT_PUBMED_20410293}}

	==About this Structure==		==About this Structure==
	~~2WSB~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA].		[[2wsb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20410293~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020410293</ref><references group="xtra"/>
	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
	[[Category: Rhodobacter sphaeroides]]		[[Category: Rhodobacter sphaeroides]]
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	[[Category: Sdr]]		[[Category: Sdr]]
	[[Category: Tagatose]]		[[Category: Tagatose]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 5 11:08:03 2010''~~

OCA at 08:08, 5 May 2010

2010-05-05T08:08:03Z

← Older revision		Revision as of 08:08, 5 May 2010
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	{{Seed}}		{{Seed}}
	[[Image:2wsb.~~jpg~~\|left\|200px]]		[[Image:2wsb.png\|left\|200px]]

	<!--		<!--
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	===CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD===		===CRYSTAL STRUCTURE OF THE SHORT-CHAIN DEHYDROGENASE GALACTITOL-DEHYDROGENASE (GATDH) OF RHODOBACTER SPHAEROIDES IN COMPLEX WITH NAD===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_20410293}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 20410293 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_20410293}}

	==About this Structure==		==About this Structure==
	2WSB is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA].		2WSB is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSB OCA].

			==Reference==
			<ref group="xtra">PMID:20410293</ref><references group="xtra"/>
	[[Category: Galactitol 2-dehydrogenase]]		[[Category: Galactitol 2-dehydrogenase]]
	[[Category: Rhodobacter sphaeroides]]		[[Category: Rhodobacter sphaeroides]]
Line 29:		Line 38:
	[[Category: Tagatose]]		[[Category: Tagatose]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 28~~ 11:01:25 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 5 11:08:03 2010''