2wxt - Revision history

OCA at 10:19, 20 December 2023

2023-12-20T10:19:19Z

← Older revision		Revision as of 10:19, 20 December 2023
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	<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus~~ perfringens~~" veillon and zuber 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [https://pdbe.org/2wxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [https://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [https://pdbe.org/2wxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [https://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1]] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.		[https://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ perfringens ~~veillon and zuber 1898~~]]		[[Category: Clostridium perfringens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Phospholipase C]]~~		[[Category: Basak AK]]
	[[Category: Basak~~, A K~~]]		[[Category: Justin N]]
	[[Category: Justin, N]]		[[Category: Naylor CE]]
	[[Category: Naylor~~, C E]]~~
	~~[[Category: C2 domain]]~~
	~~[[Category: Cytolysis]]~~
	~~[[Category: Gangrene determinant]]~~
	~~[[Category: Hemolysis]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Membrane binding]]~~
	~~[[Category: Virulence~~]]

OCA at 16:46, 3 November 2021

2021-11-03T16:46:25Z

← Older revision		Revision as of 16:46, 3 November 2021
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	<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [~~http~~://pdbe.org/2wxt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [https://pdbe.org/2wxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [https://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1]] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.		[[https://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1]] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	*[[Phospholipase C\|Phospholipase C]]		*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==

OCA at 07:35, 10 April 2019

2019-04-10T07:35:59Z

← Older revision		Revision as of 07:35, 10 April 2019
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	==Clostridium perfringens alpha-toxin strain NCTC8237==		==Clostridium perfringens alpha-toxin strain NCTC8237==
	<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus perfringens veillon and zuber 1898]]		[[Category: Bacillus perfringens veillon and zuber 1898]]
			[[Category: Large Structures]]
	[[Category: Phospholipase C]]		[[Category: Phospholipase C]]
	[[Category: Basak, A K]]		[[Category: Basak, A K]]

OCA at 09:20, 3 October 2018

2018-10-03T09:20:59Z

← Older revision		Revision as of 09:20, 3 October 2018
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	==~~CLOSTRIDIUM PERFRINGENS ALPHA~~-~~TOXIN STRAIN~~ NCTC8237==
			==Clostridium perfringens alpha-toxin strain NCTC8237==
	<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://pdbe.org/2wxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://pdbe.org/2wxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/2wxt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/2wxt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 09:26, 9 February 2016

2016-02-09T09:26:34Z

← Older revision		Revision as of 09:26, 9 February 2016
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	<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_perfringens Clostridium~~ perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://pdbe.org/2wxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wxt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2wxt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[~~Alpha-toxin~~\|~~Alpha-toxin~~]]		*[[Hemolysin\|Hemolysin]]
			*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Clostridium~~ perfringens]]		[[Category: Bacillus perfringens veillon and zuber 1898]]
	[[Category: Phospholipase C]]		[[Category: Phospholipase C]]
	[[Category: Basak, A K]]		[[Category: Basak, A K]]

OCA at 10:16, 20 January 2015

2015-01-20T10:16:56Z

← Older revision		Revision as of 10:16, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1]] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Clostridium perfringens]]		[[Category: Clostridium perfringens]]
	[[Category: Phospholipase C]]		[[Category: Phospholipase C]]
	[[Category: Basak, A K.]]		[[Category: Basak, A K]]
	[[Category: Justin, N.]]		[[Category: Justin, N]]
	[[Category: Naylor, C E.]]		[[Category: Naylor, C E]]
	[[Category: C2 domain]]		[[Category: C2 domain]]
	[[Category: Cytolysis]]		[[Category: Cytolysis]]

OCA at 08:38, 29 September 2014

2014-09-29T08:38:06Z

← Older revision		Revision as of 08:38, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2wxt~~.~~png~~\|~~left~~\|~~200px~~]]		==CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN STRAIN NCTC8237==
			<StructureSection load='2wxt' size='340' side='right' caption='[[2wxt]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qm6\|1qm6]], [[1qmd\|1qmd]], [[2wxu\|2wxu]], [[2wy6\|2wy6]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [http://www.ebi.ac.uk/pdbsum/2wxt PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/2wxt_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The alpha-toxin produced by Clostridium perfringens is one of the best-studied examples of a toxic phospholipase C. In this study, a nontoxic mutant protein from C. perfringens strain NCTC8237 in which Thr74 is substituted by isoleucine (T74I) has been characterized and is compared with the toxic wild-type protein. Thr74 is part of an exposed loop at the proposed membrane-interfacing surface of the toxin. The mutant protein had markedly reduced cytotoxic and myotoxic activities. However, this substitution did not significantly affect the catalytic activity towards water-soluble substrate or the overall three-dimensional structure of the protein. The data support the proposed role of the 70-90 loop in the recognition of membrane phospholipids. These findings also provide key evidence in support of the hypothesis that the hydrolysis of both phosphatidylcholine and sphingomyelin are required for the cytolytic and toxic activity of phospholipases.

	~~{{STRUCTURE_2wxt\| PDB=2wxt \| SCENE= }}~~		Comparison of a nontoxic variant of Clostridium perfringens alpha-toxin with the toxic wild-type strain.,Vachieri SG, Clark GC, Alape-Giron A, Flores-Diaz M, Justin N, Naylor CE, Titball RW, Basak AK Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1067-74. Epub 2010, Sep 18. PMID:20944240<ref>PMID:20944240</ref>

	~~===CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN STRAIN NCTC8237===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_20944240}}~~

	~~==About this Structure==~~
	~~[[2wxt]] is~~ a ~~1 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Clostridium_perfringens Clostridium perfringens]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=2WXT OCA].~~

	==See Also==		==See Also==
	*[[~~Gas Gangrene Toxin~~\|~~Gas Gangrene Toxin~~]]		*[[Alpha-toxin\|Alpha-toxin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:020944240<~~/~~ref~~><~~ref group="xtra">PMID:009699639</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Clostridium perfringens]]		[[Category: Clostridium perfringens]]
	[[Category: Phospholipase C]]		[[Category: Phospholipase C]]

OCA at 13:24, 20 October 2012

2012-10-20T13:24:52Z

← Older revision		Revision as of 13:24, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2wxt]] is a 1 chain structure ~~of [[Gas Gangrene Toxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA].		[[2wxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA].

	==See Also==		==See Also==

OCA at 11:52, 26 July 2012

2012-07-26T11:52:43Z

← Older revision		Revision as of 11:52, 26 July 2012
Line 1:		Line 1:
	[[Image:2wxt.png\|left\|200px]]		[[Image:2wxt.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2wxt", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2wxt\| PDB=2wxt \| SCENE= }}		{{STRUCTURE_2wxt\| PDB=2wxt \| SCENE= }}

	===CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN STRAIN NCTC8237===		===CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN STRAIN NCTC8237===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20944240}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20944240 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20944240}}		{{ABSTRACT_PUBMED_20944240}}

Line 22:		Line 11:

	==See Also==		==See Also==
	*[[Gas Gangrene Toxin]]		*[[Gas Gangrene Toxin\|Gas Gangrene Toxin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20944240~~</ref><ref group="xtra">PMID:~~9699639~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020944240</ref><ref group="xtra">PMID:009699639</ref><references group="xtra"/>
	[[Category: Clostridium perfringens]]		[[Category: Clostridium perfringens]]
	[[Category: Phospholipase C]]		[[Category: Phospholipase C]]

OCA: Protected "2wxt" [edit=sysop:move=sysop]

2010-12-27T02:34:17Z

Protected "2wxt" [edit=sysop:move=sysop]

← Older revision	Revision as of 02:34, 27 December 2010
(No difference)