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2026-06-24T04:31:25Z

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2026-06-24T04:31:25Z

← Older revision		Revision as of 04:31, 24 June 2026
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	~~'''Unreleased structure'''~~

	~~The entry~~ 36cd is ~~ON HOLD~~		==Crystal structure of CAT2==
			<StructureSection load='36cd' size='340' side='right'caption='[[36cd]], [[Resolution\|resolution]] 2.43Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[36cd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=36CD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=36CD FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.43Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=36cd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=36cd OCA], [https://pdbe.org/36cd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=36cd RCSB], [https://www.ebi.ac.uk/pdbsum/36cd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=36cd ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Monoterpene Indole Alkaloids (MIAs) are a diverse family of plant natural products with various medicinal applications. Although MIAs, such as vinblastine and reserpine, are clinically validated, sourcing of MIAs for clinical use or drug discovery from natural resources or via chemical synthesis is hampered due to their scarcity and chemical complexity. Refactoring MIA biosynthesis pathways in microbial cell factories could offer an alternative, more stable and potentially sustainable manufacturing route for alkaloid medicines and novel therapies. However, reaching commercially attractive titers, rates and yields remains challenging owing to the length and complexity of these metabolic pathways. One critical bottleneck is the low screening throughput and very high cost of the analytical methods used to quantify MIA for optimizing production. In this study, we evolved RamR, a promiscuous bacterial transcription factor to respond to five different MIAs, resulting in highly sensitive and selective sensor variants (EC50<10 muM). X-ray crystallography and computational modeling provided insight into the MIA binding of the evolved biosensor variants. The RamR biosensing platform was functionalized in yeast and subsequently applied in a cost-effective semi-throughput screening campaign of a 188-gene overexpression library to identify high-performing cell factory designs for strictosidine, the common precursor for all MIAs. The fluorescent biosensor signal correlated with HPLC quantification (r2 = 0.932) allowing identification of single metabolic engineering hits which when combined yielded a maximum titer of >220 mg/L strictosidine, 3-fold higher than the parental reference strain. This study demonstrates the development of selective biosensors for MIAs and the cost-effective identification of novel metabolic engineering hits for optimizing MIA production in microbial cell factories.

	~~Authors:~~ Hong, Y., Zhang, Y.J.		Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast.,Holtz M, d'Oelsnitz S, Domingo CC, Madsen NG, Hong MY, Arnesen JA, van Aalst ACA, de Haan S, Weingarten CK, Welner DH, Silver PA, Zhang YJ, Jensen MK, Acevedo-Rocha CG bioRxiv [Preprint]. 2026 Jun 1:2026.06.01.729246. doi: , 10.64898/2026.06.01.729246. PMID:42282776<ref>PMID:42282776</ref>

	~~Description~~: ~~Crystal structure of CAT2~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: Hong, Y]]		<div class="pdbe-citations 36cd" style="background-color:#fffaf0;"></div>
	[[Category: Zhang~~, Y.J~~]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
			[[Category: Hong Y]]
			[[Category: Zhang YJ]]

OCA: Created page with "'''Unreleased structure''' The entry 36cd is ON HOLD Authors: Hong, Y., Zhang, Y.J. Description: Crystal structure of CAT2 Category: Unreleased Structures Category: Hong, Y Category: Zhang, Y.J"

2026-06-10T14:42:41Z

Created page with "'''Unreleased structure''' The entry 36cd is ON HOLD Authors: Hong, Y., Zhang, Y.J. Description: Crystal structure of CAT2 Category: Unreleased Structures Category: Hong, Y Category: Zhang, Y.J"

New page

'''Unreleased structure'''

The entry 36cd is ON HOLD

Authors: Hong, Y., Zhang, Y.J.

Description: Crystal structure of CAT2
[[Category: Unreleased Structures]]
[[Category: Hong, Y]]
[[Category: Zhang, Y.J]]

36cd - Revision history

OCA: Protected "36cd": Seeded page ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

OCA at 04:31, 24 June 2026

OCA: Created page with "'''Unreleased structure''' The entry 36cd is ON HOLD Authors: Hong, Y., Zhang, Y.J. Description: Crystal structure of CAT2 Category: Unreleased Structures Category: Hong, Y Category: Zhang, Y.J"