3alg - Revision history

OCA at 15:48, 4 October 2023

2023-10-04T15:48:19Z

← Older revision		Revision as of 15:48, 4 October 2023
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	<StructureSection load='3alg' size='340' side='right'caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3alg' size='340' side='right'caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~American_tobacco American tobacco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ALG FirstGlance]. <br>		<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ALG FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3alf\|3alf]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">chi~~-~~V ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td~~></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [https://pdbe.org/3alg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [https://www.ebi.ac.uk/pdbsum/3alg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3alg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [https://pdbe.org/3alg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [https://www.ebi.ac.uk/pdbsum/3alg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3alg ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q43576_TOBAC Q43576_TOBAC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: American tobacco]]~~
	~~[[Category: Chitinase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Fukamizo, T~~]]		[[Category: Nicotiana tabacum]]
	[[Category: ~~Numata,~~ T]]		[[Category: Fukamizo T]]
	[[Category: ~~Ohnuma,~~ T]]		[[Category: Numata T]]
	[[Category: ~~Osawa,~~ T]]		[[Category: Ohnuma T]]
	[[Category: ~~Hydrolase~~]]		[[Category: Osawa T]]

OCA at 14:56, 29 December 2021

2021-12-29T14:56:33Z

← Older revision		Revision as of 14:56, 29 December 2021
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	==Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4==		==Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4==
	<StructureSection load='3alg' size='340' side='right' caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3alg' size='340' side='right'caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ALG FirstGlance]. <br>		<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ALG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL-D-GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=~~PO4~~:~~PHOSPHATE+ION~~'>~~PO4~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3alf\|3alf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [~~http~~://pdbe.org/3alg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3alg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3alg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [https://pdbe.org/3alg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [https://www.ebi.ac.uk/pdbsum/3alg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3alg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Chitinase\|Chitinase]]		*[[Chitinase 3D structures\|Chitinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: American tobacco]]		[[Category: American tobacco]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
			[[Category: Large Structures]]
	[[Category: Fukamizo, T]]		[[Category: Fukamizo, T]]
	[[Category: Numata, T]]		[[Category: Numata, T]]

OCA at 07:52, 24 October 2018

2018-10-24T07:52:02Z

← Older revision		Revision as of 07:52, 24 October 2018
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ALG FirstGlance]. <br>		<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ALG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=~~PO4~~:~~PHOSPHATE+ION~~'>~~PO4~~</scene>, <scene name='pdbligand=~~NAG~~:~~N-ACETYL-D-GLUCOSAMINE~~'>~~NAG~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>

OCA at 11:06, 11 August 2016

2016-08-11T11:06:52Z

← Older revision		Revision as of 11:06, 11 August 2016
Line 1:		Line 1:

	==Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4==		==Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4==
	<StructureSection load='3alg' size='340' side='right' caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3alg' size='340' side='right' caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nicotiana_tabacum Nicotiana tabacum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ALG FirstGlance]. <br>		<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ALG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 ~~Nicotiana tabacum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [http://www.ebi.ac.uk/pdbsum/3alg PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [http://pdbe.org/3alg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [http://www.ebi.ac.uk/pdbsum/3alg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3alg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3alg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: American tobacco]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
	~~[[Category: Nicotiana tabacum]]~~
	[[Category: Fukamizo, T]]		[[Category: Fukamizo, T]]
	[[Category: Numata, T]]		[[Category: Numata, T]]

OCA at 09:13, 25 January 2015

2015-01-25T09:13:04Z

← Older revision		Revision as of 09:13, 25 January 2015
Line 26:		Line 26:
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Nicotiana tabacum]]		[[Category: Nicotiana tabacum]]
	[[Category: Fukamizo, T.]]		[[Category: Fukamizo, T]]
	[[Category: Numata, T.]]		[[Category: Numata, T]]
	[[Category: Ohnuma, T.]]		[[Category: Ohnuma, T]]
	[[Category: Osawa, T~~.]]~~		[[Category: Osawa, T]]
	~~[[Category: Chitinase~~]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:19, 5 November 2014

2014-11-05T09:19:02Z

← Older revision		Revision as of 09:19, 5 November 2014
Line 1:		Line 1:
	[[~~Image~~:~~3alg~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4==
			<StructureSection load='3alg' size='340' side='right' caption='[[3alg]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ALG FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3alf\|3alf]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">chi-V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 Nicotiana tabacum])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3alg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3alg RCSB], [http://www.ebi.ac.uk/pdbsum/3alg PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			A class V chitinase from Nicotiana tabacum (NtChiV) with amino acid sequence similar to that of Serratia marcescens chitinase B (SmChiB) was expressed in E. coli and purified to homogeneity. When N-acetylglucosamine oligosaccharides [(NAG)(n)] were hydrolyzed by the purified NtChiV, the second glycosidic linkage from the non-reducing end was predominantly hydrolyzed in a manner similar to that of SmChiB. NtChiV was shown to hydrolyze partially N-acetylated chitosan non-processively, whereas SmChiB hydrolyzes the same substrate processively. The crystal structure of NtChiV was determined by the single-wavelength anomalous dispersion method at 1.2 A resolution. The protein adopts a classical (beta/alpha)-barrel fold (residues 1-233 and 303-348) with an insertion of a small (alpha + beta) domain (residues 234-302). This is the first crystal structure of a plant class V chitinase. The crystal structure of the inactive mutant NtChiV E115Q complexed with (NAG) was also solved and exhibited a linear conformation of the bound oligosaccharide occupying -2, +1, +2, and +3 subsites. The complex structure corresponds to an initial state of (NAG) binding, which is proposed to be converted into a bent conformation through sliding of the +1, +2, and +3 sugar units to -1, +1, and +2 subsites. Although NtChiV is similar to SmChiB, the chitin-binding domain is present in the C-terminus of the latter, but not in the former. Aromatic amino acid residues found in the substrate binding cleft of SmChiB, including Trp97, are substituted with aliphatic residues in NtChiV. These structural differences appear to be responsible for NtChiV being a non-processive enzyme.

	~~{{STRUCTURE_3alg\| PDB=3alg \| SCENE= }}~~		Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum.,Ohnuma T, Numata T, Osawa T, Mizuhara M, Varum KM, Fukamizo T Plant Mol Biol. 2011 Feb;75(3):291-304. Epub 2011 Jan 15. PMID:21240541<ref>PMID:21240541</ref>

	~~===Crystal Structure~~ of ~~Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21240541}}~~

	~~==About this Structure==~~
	~~[[3alg]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Nicotiana_tabacum Nicotiana tabacum]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3ALG OCA].~~

	==See Also==		==See Also==
	*[[Chitinase\|Chitinase]]		*[[Chitinase\|Chitinase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:021240541<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Nicotiana tabacum]]		[[Category: Nicotiana tabacum]]

OCA at 06:27, 20 October 2012

2012-10-20T06:27:14Z

← Older revision		Revision as of 06:27, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3alg]] is a 1 chain structure ~~of [[Chitinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA].		[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA].

	==See Also==		==See Also==

OCA at 13:13, 25 July 2012

2012-07-25T13:13:58Z

← Older revision		Revision as of 13:13, 25 July 2012
Line 1:		Line 1:
	[[Image:3alg.~~jpg~~\|left\|200px]]		[[Image:3alg.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3alg", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3alg\| PDB=3alg \| SCENE= }}		{{STRUCTURE_3alg\| PDB=3alg \| SCENE= }}

	===Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4===		===Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21240541}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21240541 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21240541}}		{{ABSTRACT_PUBMED_21240541}}

	==About this Structure==		==About this Structure==
	[[3alg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA].		[[3alg]] is a 1 chain structure of [[Chitinase]] with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALG OCA].

			==See Also==
			*[[Chitinase\|Chitinase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21240541~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021240541</ref><references group="xtra"/>
	[[Category: Chitinase]]		[[Category: Chitinase]]
	[[Category: Nicotiana tabacum]]		[[Category: Nicotiana tabacum]]
Line 29:		Line 21:
	[[Category: Ohnuma, T.]]		[[Category: Ohnuma, T.]]
	[[Category: Osawa, T.]]		[[Category: Osawa, T.]]
			[[Category: Chitinase]]
			[[Category: Hydrolase]]

OCA: Protected "3alg" [edit=sysop:move=sysop]

2011-03-23T07:03:09Z

Protected "3alg" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:03, 23 March 2011
(No difference)

OCA at 07:03, 23 March 2011

2011-03-23T07:03:04Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3alg.jpg|left|200px]]
-The entry 3alg is ON HOLD  until Paper Publication
+<!--
-Authors: Numata, T., Osawa, T., Ohnuma, T., Fukamizo, T.
+===Crystal Structure of Class V Chitinase (E115Q mutant) from Nicotiana tobaccum in complex with NAG4===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep  1 09:30:02 2010''
+<!--