3b60 - Revision history

OCA at 09:27, 21 February 2024

2024-02-21T09:27:45Z

← Older revision		Revision as of 09:27, 21 February 2024
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	<StructureSection load='3b60' size='340' side='right'caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3b60' size='340' side='right'caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3B60 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~ADENYLATE+ESTER'>ANP</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.7Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [~~http~~://pdbe.org/3b60 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [https://pdbe.org/3b60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [https://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/MSBA_SALTY MSBA_SALTY]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).		[https://www.uniprot.org/uniprot/MSBA_SALTY MSBA_SALTY] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b60 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b60 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.

	Flexibility in the ABC transporter MsbA: Alternating access with a twist.,Ward A, Reyes CL, Yu J, Roth CB, Chang G Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:18024585<ref>PMID:18024585</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3b60" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chang, G]]		[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
	[[Category: Reyes~~, C L~~]]		[[Category: Chang G]]
	[[Category: Roth~~, C B~~]]		[[Category: Reyes CL]]
	[[Category: Ward, A]]		[[Category: Roth CB]]
	[[Category: Yu, J]]		[[Category: Ward A]]
	~~[[Category: Abc transporter]]~~		[[Category: Yu J]]
	~~[[Category: Atp-binding]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Inner membrane]]~~
	~~[[Category: Lipid flippase]]~~
	~~[[Category: Lipid transport]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Msba]]~~
	~~[[Category: Nucleotide-binding]]~~
	~~[[Category: Transmembrane~~]]

OCA at 06:15, 5 August 2020

2020-08-05T06:15:29Z

← Older revision		Revision as of 06:15, 5 August 2020
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	==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==		==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==
	<StructureSection load='3b60' size='340' side='right' caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3b60' size='340' side='right'caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3B60 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://pdbe.org/3b60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://pdbe.org/3b60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Chang, G]]		[[Category: Chang, G]]
	[[Category: Reyes, C L]]		[[Category: Reyes, C L]]

OCA at 07:41, 31 October 2018

2018-10-31T07:41:29Z

← Older revision		Revision as of 07:41, 31 October 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b60_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b60_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:14, 5 August 2016

2016-08-05T08:14:47Z

← Older revision		Revision as of 08:14, 5 August 2016
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	==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==		==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==
	<StructureSection load='3b60' size='340' side='right' caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3b60' size='340' side='right' caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B60 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://pdbe.org/3b60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b60 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3b60" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]~~
	[[Category: Chang, G]]		[[Category: Chang, G]]
	[[Category: Reyes, C L]]		[[Category: Reyes, C L]]

OCA at 17:11, 25 December 2014

2014-12-25T17:11:22Z

← Older revision		Revision as of 17:11, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B60 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MSBA_SALTY MSBA_SALTY]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Chang, G.]]		[[Category: Chang, G]]
	[[Category: Reyes, C L.]]		[[Category: Reyes, C L]]
	[[Category: Roth, C B.]]		[[Category: Roth, C B]]
	[[Category: Ward, A.]]		[[Category: Ward, A]]
	[[Category: Yu, J.]]		[[Category: Yu, J]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 04:51, 2 October 2014

2014-10-02T04:51:46Z

← Older revision		Revision as of 04:51, 2 October 2014
Line 1:		Line 1:
	[[Image:~~3b60~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==
			<StructureSection load='3b60' size='340' side='right' caption='[[3b60]], [[Resolution\|resolution]] 3.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3b5w\|3b5w]], [[3b5x\|3b5x]], [[3b5y\|3b5y]], [[3b5z\|3b5z]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [http://www.ebi.ac.uk/pdbsum/3b60 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b60_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.

	~~{{STRUCTURE_3b60\| PDB=3b60 \| SCENE= }}~~		Flexibility in the ABC transporter MsbA: Alternating access with a twist.,Ward A, Reyes CL, Yu J, Roth CB, Chang G Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:18024585<ref>PMID:18024585</ref>

	~~===Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP~~, ~~higher resolution form===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_18024585}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3b60]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Salmonella_enterica_subsp~~.~~_enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:018024585<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]

OCA: Protected "3b60" [edit=sysop:move=sysop]

2013-01-07T20:44:23Z

Protected "3b60" [edit=sysop:move=sysop]

← Older revision	Revision as of 20:44, 7 January 2013
(No difference)

OCA at 20:44, 7 January 2013

2013-01-07T20:44:22Z

← Older revision		Revision as of 20:44, 7 January 2013
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	~~{{Seed}}~~
	[[Image:3b60.png\|left\|200px]]		[[Image:3b60.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3b60", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3b60\| PDB=3b60 \| SCENE= }}		{{STRUCTURE_3b60\| PDB=3b60 \| SCENE= }}

	===Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form===		===Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_18024585}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 18024585 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_18024585}}		{{ABSTRACT_PUBMED_18024585}}

	==About this Structure==		==About this Structure==
	~~3B60~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Salmonella_typhimurium~~ Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].		[[3b60]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18024585~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018024585</ref><references group="xtra"/>
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Chang, G.]]		[[Category: Chang, G.]]
	[[Category: Reyes, C L.]]		[[Category: Reyes, C L.]]
Line 41:		Line 29:
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:48:43 2009''~~

OCA at 21:48, 17 February 2009

2009-02-17T21:48:46Z

← Older revision		Revision as of 21:48, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	3B60 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].		3B60 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].

	==Reference==		==Reference==
	~~Flexibility in the ABC transporter MsbA: Alternating access with a twist., Ward A, Reyes CL, Yu J, Roth CB, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~18024585 18024585]~~		<ref group="xtra">PMID:18024585</ref><references group="xtra"/>
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella typhimurium]]
	~~[[Category: Single protein]]~~
	[[Category: Chang, G.]]		[[Category: Chang, G.]]
	[[Category: Reyes, C L.]]		[[Category: Reyes, C L.]]
Line 43:		Line 42:
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 07~~:01:~~20 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:48:43 2009''

OCA at 04:01, 29 July 2008

2008-07-29T04:01:26Z

← Older revision		Revision as of 04:01, 29 July 2008
Line 1:		Line 1:
	[[Image:3b60.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:3b60.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_3b60\| PDB=3b60 \| SCENE= }}		{{STRUCTURE_3b60\| PDB=3b60 \| SCENE= }}

	~~'''~~Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form~~'''~~		===Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form===


	~~==Overview==~~		<!--
	~~ATP~~-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-~~ray structures of the bacterial ABC lipid flippase~~, ~~MsbA~~, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the ~~nucleotide-binding domains~~ to ~~disassociate while~~ the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (~~inward) facing to extracellular (outward) facing~~. ~~The inward and outward openings are mediated by two different sets of transmembrane helix interactions~~. ~~Altogether~~, the ~~conformational changes between these structures suggest that large ranges of motion may be required for substrate transport~~.		The line below this paragraph, {{ABSTRACT_PUBMED_18024585}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 18024585 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_18024585}}

	==About this Structure==		==About this Structure==
Line 38:		Line 42:
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:01:20 2008''