3c8a - Revision history

OCA at 08:52, 30 October 2024

2024-10-30T08:52:30Z

← Older revision		Revision as of 08:52, 30 October 2024
Line 3:		Line 3:
	<StructureSection load='3c8a' size='340' side='right'caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='3c8a' size='340' side='right'caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C8A FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.52Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]]~~, ~~[[3c8b\|3c8b]]~~<~~/div></td></tr>~~
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">botA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>~~
	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [https://pdbe.org/3c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [https://www.ebi.ac.uk/pdbsum/3c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [https://pdbe.org/3c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [https://www.ebi.ac.uk/pdbsum/3c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.		[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 15:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8a_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
Line 39:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~		[[Category: Clostridium botulinum]]
	~~[[Category: Bontoxilysin~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Kumaran, D~~]]		[[Category: Synthetic construct]]
	[[Category: ~~Swaminathan, S~~]]		[[Category: Kumaran D]]
	[[Category: ~~Bio-warfare agent]]~~		[[Category: Swaminathan S]]
	~~[[Category: Botulinum neurotoxin type some]]~~
	~~[[Category: Catalytic domain]]~~
	~~[[Category: Endopeptidase]]~~
	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~
	~~[[Category: Metalloprotease]]~~
	~~[[Category: Protease]]~~
	~~[[Category: Secreted~~]]

OCA at 07:46, 27 January 2022

2022-01-27T07:46:47Z

← Older revision		Revision as of 07:46, 27 January 2022
Line 1:		Line 1:

	==Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL==		==Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL==
	<StructureSection load='3c8a' size='340' side='right' caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='3c8a' size='340' side='right'caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3C8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C8A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [~~http~~://pdbe.org/3c8a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3c8a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [https://pdbe.org/3c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [https://www.ebi.ac.uk/pdbsum/3c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.		[[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:

	==See Also==		==See Also==
	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]		*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 41:		Line 41:
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
			[[Category: Large Structures]]
	[[Category: Kumaran, D]]		[[Category: Kumaran, D]]
	[[Category: Swaminathan, S]]		[[Category: Swaminathan, S]]

OCA at 13:32, 7 November 2018

2018-11-07T13:32:03Z

← Older revision		Revision as of 13:32, 7 November 2018
Line 17:		Line 17:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8a_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:22, 5 August 2016

2016-08-05T04:22:30Z

← Older revision		Revision as of 04:22, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL==		==Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL==
	<StructureSection load='3c8a' size='340' side='right' caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>		<StructureSection load='3c8a' size='340' side='right' caption='[[3c8a]], [[Resolution\|resolution]] 1.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C8A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 ~~Clostridium botulinum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [http://www.ebi.ac.uk/pdbsum/3c8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [http://pdbe.org/3c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [http://www.ebi.ac.uk/pdbsum/3c8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c8a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3c8a" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	~~[[Category: Clostridium botulinum]]~~
	[[Category: Kumaran, D]]		[[Category: Kumaran, D]]
	[[Category: Swaminathan, S]]		[[Category: Swaminathan, S]]

OCA at 15:47, 25 December 2014

2014-12-25T15:47:15Z

← Older revision		Revision as of 15:47, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C8A FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bwi\|3bwi]], [[3c88\|3c88]], [[3c89\|3c89]], [[3c8b\|3c8b]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [http://www.ebi.ac.uk/pdbsum/3c8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c8a RCSB], [http://www.ebi.ac.uk/pdbsum/3c8a PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Kumaran, D.]]		[[Category: Kumaran, D]]
	[[Category: Swaminathan, S.]]		[[Category: Swaminathan, S]]
	[[Category: Bio-warfare agent]]		[[Category: Bio-warfare agent]]
	[[Category: Botulinum neurotoxin type some]]		[[Category: Botulinum neurotoxin type some]]

OCA at 21:05, 2 October 2014

2014-10-02T21:05:19Z

@@ Line 1: / Line 1: @@
-[[Image:3c8a.png|left|200px]]
+==Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL==
-{{STRUCTURE_3c8a|  PDB=3c8a  |  SCENE=  }}
+Structure- and substrate-based inhibitor design for Clostridium botulinum neurotoxin serotype A.,Kumaran D, Rawat R, Ludivico ML, Ahmed SA, Swaminathan S J Biol Chem. 2008 Jul 4;283(27):18883-91. Epub 2008 Apr 22. PMID:18434312<ref>PMID:18434312</ref>
-===Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_18434312}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].
+</StructureSection>
 [[Category: Bontoxilysin]]
 [[Category: Clostridium botulinum]]

OCA: Protected "3c8a" [edit=sysop:move=sysop]

2013-01-07T18:42:31Z

Protected "3c8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 18:42, 7 January 2013
(No difference)

OCA at 18:42, 7 January 2013

2013-01-07T18:42:26Z

← Older revision		Revision as of 18:42, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:3c8a.png\|left\|200px]]		[[Image:3c8a.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3c8a", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3c8a\| PDB=3c8a \| SCENE= }}		{{STRUCTURE_3c8a\| PDB=3c8a \| SCENE= }}

	===Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL===		===Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_18434312}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 18434312 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_18434312}}		{{ABSTRACT_PUBMED_18434312}}

	==About this Structure==		==About this Structure==
	~~3C8A~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].		[[3c8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18434312~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018434312</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
Line 32:		Line 20:
	[[Category: Catalytic domain]]		[[Category: Catalytic domain]]
	[[Category: Endopeptidase]]		[[Category: Endopeptidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Metalloprotease]]		[[Category: Metalloprotease]]
	~~[[Category: Pharmaceutical]]~~
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Secreted]]		[[Category: Secreted]]
	~~[[Category: Zinc]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:52:16 2009''~~

OCA at 18:52, 17 February 2009

2009-02-17T18:52:18Z

← Older revision		Revision as of 18:52, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	3C8A is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].		3C8A is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].

	==Reference==		==Reference==
	Structure- and substrate-based inhibitor design for Clostridium botulinum neurotoxin serotype A., Kumaran D, Rawat R, Ludivico ML, Ahmed SA, Swaminathan S, J Biol Chem. 2008 Jul 4;283(27):18883-91. Epub 2008 Apr 22. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18434312 18434312]~~		<ref group="xtra">PMID:18434312</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	~~[[Category: Single protein]]~~
	[[Category: Kumaran, D.]]		[[Category: Kumaran, D.]]
	[[Category: Swaminathan, S.]]		[[Category: Swaminathan, S.]]
Line 40:		Line 39:
	[[Category: Zinc]]		[[Category: Zinc]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 23 10~~:08:~~36 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:52:16 2009''

OCA at 07:08, 23 July 2008

2008-07-23T07:08:37Z

← Older revision		Revision as of 07:08, 23 July 2008
Line 1:		Line 1:
	[[Image:3c8a.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:3c8a.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3c8a\| PDB=3c8a \| SCENE= }}		{{STRUCTURE_3c8a\| PDB=3c8a \| SCENE= }}

	~~'''~~Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL~~'''~~		===Crystal structure of the catalytic domain of botulinum neurotoxin serotype A with inhibitory peptide RRGL===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_18434312}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 18434312 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_18434312}}

	==About this Structure==		==About this Structure==
	3C8A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].		3C8A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8A OCA].

			==Reference==
			Structure- and substrate-based inhibitor design for Clostridium botulinum neurotoxin serotype A., Kumaran D, Rawat R, Ludivico ML, Ahmed SA, Swaminathan S, J Biol Chem. 2008 Jul 4;283(27):18883-91. Epub 2008 Apr 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18434312 18434312]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
Line 29:		Line 39:
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Zinc]]		[[Category: Zinc]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Apr 24 09~~:53:05 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:08:36 2008''