3ch8 - Revision history

OCA at 09:35, 21 February 2024

2024-02-21T09:35:29Z

← Older revision		Revision as of 09:35, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3ch8' size='340' side='right'caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3ch8' size='340' side='right'caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [~~http~~://pdbe.org/3ch8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ch8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ch8 ProSAT]</span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [https://pdbe.org/3ch8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ch8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ch8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~LAP2_HUMAN LAP2_HUMAN]~~] Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.<ref>PMID:10878805</ref>		[https://www.uniprot.org/uniprot/ERBIN_HUMAN ERBIN_HUMAN] Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state (PubMed:16203728). Inhibits NOD2-dependent NF-kappa-B signaling and proinflammatory cytokine secretion (PubMed:16203728).<ref>PMID:10878805</ref> <ref>PMID:16203728</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ch8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ch8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	We have established a new protein-engineering strategy termed "directed domain-interface evolution" that generates a binding site by linking two protein domains and then optimizing the interface between them. Using this strategy, we have generated synthetic two-domain "affinity clamps" using PDZ and fibronectin type III (FN3) domains as the building blocks. While these affinity clamps all had significantly higher affinity toward a target peptide than the underlying PDZ domain, two distinct types of affinity clamps were found in terms of target specificity. One type conserved the specificity of the parent PDZ domain, and the other increased the specificity dramatically. Here, we characterized their specificity profiles using peptide phage-display libraries and scanning mutagenesis, which suggested a significantly enlarged recognition site of the high-specificity affinity clamps. The crystal structure of a high-specificity affinity clamp showed extensive contacts with a portion of the peptide ligand that is not recognized by the parent PDZ domain, thus rationalizing the improvement of the specificity of the affinity clamp. A comparison with another affinity clamp structure showed that, although both had extensive contacts between PDZ and FN3 domains, they exhibited a large offset in the relative position of the two domains. Our results indicate that linked domains could rapidly fuse and evolve as a single functional module, and that the inherent plasticity of domain interfaces allows for the generation of diverse active-site topography. These attributes of directed domain-interface evolution provide facile means to generate synthetic proteins with a broad range of functions.

	Structural basis for exquisite specificity of affinity clamps, synthetic binding proteins generated through directed domain-interface evolution.,Huang J, Makabe K, Biancalana M, Koide A, Koide S J Mol Biol. 2009 Oct 9;392(5):1221-31. Epub 2009 Jul 30. PMID:19646997<ref>PMID:19646997</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3ch8" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
Line 37:		Line 29:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Huang, J]]		[[Category: Huang J]]
	[[Category: Koide, A]]		[[Category: Koide A]]
	[[Category: Koide, S]]		[[Category: Koide S]]
	[[Category: Makabe, K]]		[[Category: Makabe K]]
	~~[[Category: Fibronectin]]~~
	~~[[Category: Pdz]]~~
	~~[[Category: Structural protein~~]]

OCA at 10:27, 12 August 2020

2020-08-12T10:27:11Z

← Older revision		Revision as of 10:27, 12 August 2020
Line 1:		Line 1:

	==The crystal structure of PDZ-Fibronectin fusion protein==		==The crystal structure of PDZ-Fibronectin fusion protein==
	<StructureSection load='3ch8' size='340' side='right' caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3ch8' size='340' side='right'caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://pdbe.org/3ch8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ch8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://pdbe.org/3ch8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ch8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:

	==See Also==		==See Also==
	*[[Fibronectin\|Fibronectin]]		*[[Fibronectin 3D structures\|Fibronectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
			[[Category: Large Structures]]
	[[Category: Huang, J]]		[[Category: Huang, J]]
	[[Category: Koide, A]]		[[Category: Koide, A]]

OCA at 13:54, 7 November 2018

2018-11-07T13:54:22Z

← Older revision		Revision as of 13:54, 7 November 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3ch8_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3ch8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:14, 5 August 2016

2016-08-05T06:14:34Z

← Older revision		Revision as of 06:14, 5 August 2016
Line 1:		Line 1:

	==The crystal structure of PDZ-Fibronectin fusion protein==		==The crystal structure of PDZ-Fibronectin fusion protein==
	<StructureSection load='3ch8' size='340' side='right' caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3ch8' size='340' side='right' caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://pdbe.org/3ch8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ch8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ch8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ch8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:26, 25 December 2014

2014-12-25T16:26:15Z

← Older revision		Revision as of 16:26, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LAP2_HUMAN LAP2_HUMAN]] Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.<ref>PMID:10878805</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Huang, J.]]		[[Category: Huang, J]]
	[[Category: Koide, A.]]		[[Category: Koide, A]]
	[[Category: Koide, S.]]		[[Category: Koide, S]]
	[[Category: Makabe, K.]]		[[Category: Makabe, K]]
	[[Category: Fibronectin]]		[[Category: Fibronectin]]
	[[Category: Pdz]]		[[Category: Pdz]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

OCA at 09:13, 29 September 2014

2014-09-29T09:13:47Z

← Older revision		Revision as of 09:13, 29 September 2014
Line 1:		Line 1:
	[[Image:~~3ch8~~.~~png~~\|~~left~~\|~~200px~~]]		==The crystal structure of PDZ-Fibronectin fusion protein==
			<StructureSection load='3ch8' size='340' side='right' caption='[[3ch8]], [[Resolution\|resolution]] 1.90Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CH8 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ch8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ch8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ch8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ch8 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/3ch8_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We have established a new protein-engineering strategy termed "directed domain-interface evolution" that generates a binding site by linking two protein domains and then optimizing the interface between them. Using this strategy, we have generated synthetic two-domain "affinity clamps" using PDZ and fibronectin type III (FN3) domains as the building blocks. While these affinity clamps all had significantly higher affinity toward a target peptide than the underlying PDZ domain, two distinct types of affinity clamps were found in terms of target specificity. One type conserved the specificity of the parent PDZ domain, and the other increased the specificity dramatically. Here, we characterized their specificity profiles using peptide phage-display libraries and scanning mutagenesis, which suggested a significantly enlarged recognition site of the high-specificity affinity clamps. The crystal structure of a high-specificity affinity clamp showed extensive contacts with a portion of the peptide ligand that is not recognized by the parent PDZ domain, thus rationalizing the improvement of the specificity of the affinity clamp. A comparison with another affinity clamp structure showed that, although both had extensive contacts between PDZ and FN3 domains, they exhibited a large offset in the relative position of the two domains. Our results indicate that linked domains could rapidly fuse and evolve as a single functional module, and that the inherent plasticity of domain interfaces allows for the generation of diverse active-site topography. These attributes of directed domain-interface evolution provide facile means to generate synthetic proteins with a broad range of functions.

	~~{{STRUCTURE_3ch8\| PDB=3ch8 \| SCENE= }}~~		Structural basis for exquisite specificity of affinity clamps, synthetic binding proteins generated through directed domain-interface evolution.,Huang J, Makabe K, Biancalana M, Koide A, Koide S J Mol Biol. 2009 Oct 9;392(5):1221-31. Epub 2009 Jul 30. PMID:19646997<ref>PMID:19646997</ref>

	~~===The crystal structure~~ of ~~PDZ-Fibronectin fusion protein===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19646997}}~~

	~~==About this Structure==~~
	~~[[3ch8]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3CH8 OCA].~~

	==See Also==		==See Also==
	*[[Fibronectin\|Fibronectin]]		*[[Fibronectin\|Fibronectin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019646997<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Huang, J.]]		[[Category: Huang, J.]]

OCA at 18:53, 20 October 2012

2012-10-20T18:53:03Z

← Older revision		Revision as of 18:53, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3ch8]] is a 2 chain structure ~~of [[Fibronectin]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA].		[[3ch8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA].

	==See Also==		==See Also==

OCA at 05:34, 27 July 2012

2012-07-27T05:34:30Z

← Older revision		Revision as of 05:34, 27 July 2012
Line 1:		Line 1:
	[[Image:3ch8.png\|left\|200px]]		[[Image:3ch8.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3ch8", creates the "Structure Box" on the page.~~
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	{{STRUCTURE_3ch8\| PDB=3ch8 \| SCENE= }}		{{STRUCTURE_3ch8\| PDB=3ch8 \| SCENE= }}

	===The crystal structure of PDZ-Fibronectin fusion protein===		===The crystal structure of PDZ-Fibronectin fusion protein===


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	{{ABSTRACT_PUBMED_19646997}}		{{ABSTRACT_PUBMED_19646997}}

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	==See Also==		==See Also==
	*[[Fibronectin]]		*[[Fibronectin\|Fibronectin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19646997~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019646997</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Huang, J.]]		[[Category: Huang, J.]]

OCA: Protected "3ch8" [edit=sysop:move=sysop]

2010-12-26T21:57:11Z

Protected "3ch8" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:57, 26 December 2010
(No difference)

OCA at 21:57, 26 December 2010

2010-12-26T21:57:10Z

← Older revision		Revision as of 21:57, 26 December 2010
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	~~{{Seed}}~~
	[[Image:3ch8.png\|left\|200px]]		[[Image:3ch8.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
	~~3CH8~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA].		[[3ch8]] is a 2 chain structure of [[Fibronectin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CH8 OCA].

			==See Also==
			*[[Fibronectin]]

	==Reference==		==Reference==
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	[[Category: Pdz]]		[[Category: Pdz]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 27 15:30:42 2010''~~