3dft - Revision history

OCA at 12:46, 30 August 2023

2023-08-30T12:46:32Z

← Older revision		Revision as of 12:46, 30 August 2023
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	<StructureSection load='3dft' size='340' side='right'caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3dft' size='340' side='right'caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~European_rabbit European rabbit~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DFT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DFT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat~~">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3dfn\|3dfn]]~~, [[~~3dfo~~\|~~3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs~~]]~~</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ALDOA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9986 European rabbit])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [https://pdbe.org/3dft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [https://www.ebi.ac.uk/pdbsum/3dft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dft ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [https://pdbe.org/3dft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [https://www.ebi.ac.uk/pdbsum/3dft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dft ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>		[https://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: European rabbit]]~~
	~~[[Category: Fructose-bisphosphate aldolase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~St-Jean, M~~]]		[[Category: Oryctolagus cuniculus]]
	[[Category: ~~Sygusch, J~~]]		[[Category: St-Jean M]]
	[[Category: ~~Acetylation]]~~		[[Category: Sygusch J]]
	~~[[Category: Aldolase]]~~
	~~[[Category: Glycolysis]]~~
	~~[[Category: Ion]]~~
	~~[[Category: Lyase]]~~
	~~[[Category: Mutant]]~~
	~~[[Category: Phosphate]]~~
	~~[[Category: Phosphoprotein]]~~
	~~[[Category: Schiff base~~]]

OCA at 19:23, 20 October 2021

2021-10-20T19:23:18Z

← Older revision		Revision as of 19:23, 20 October 2021
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	==Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle==		==Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle==
	<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3dft' size='340' side='right'caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3DFT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DFT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [~~http~~://pdbe.org/3dft PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3dft PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3dft ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [https://pdbe.org/3dft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [https://www.ebi.ac.uk/pdbsum/3dft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dft ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>		[[https://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase 3D structures\|Aldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: European rabbit]]		[[Category: European rabbit]]
	[[Category: Fructose-bisphosphate aldolase]]		[[Category: Fructose-bisphosphate aldolase]]
			[[Category: Large Structures]]
	[[Category: St-Jean, M]]		[[Category: St-Jean, M]]
	[[Category: Sygusch, J]]		[[Category: Sygusch, J]]

OCA at 09:56, 14 November 2018

2018-11-14T09:56:06Z

← Older revision		Revision as of 09:56, 14 November 2018
Line 1:		Line 1:

	==Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle==		==Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle==
	<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://pdbe.org/3dft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://pdbe.org/3dft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dft ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/df/3dft_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/df/3dft_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: St-Jean, M]]		[[Category: St-Jean, M]]
	[[Category: Sygusch, J]]		[[Category: Sygusch, J]]
			[[Category: Acetylation]]
	[[Category: Aldolase]]		[[Category: Aldolase]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]

OCA at 19:26, 9 February 2016

2016-02-09T19:26:45Z

← Older revision		Revision as of 19:26, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Oryctolagus_cuniculus Oryctolagus cuniculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DFT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DFT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 ~~Oryctolagus cuniculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://pdbe.org/3dft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dft ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3dft" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: European rabbit]]
	[[Category: Fructose-bisphosphate aldolase]]		[[Category: Fructose-bisphosphate aldolase]]
	~~[[Category: Oryctolagus cuniculus]]~~
	[[Category: St-Jean, M]]		[[Category: St-Jean, M]]
	[[Category: Sygusch, J]]		[[Category: Sygusch, J]]

OCA at 09:01, 25 December 2014

2014-12-25T09:01:33Z

← Older revision		Revision as of 09:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DFT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DFT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 Oryctolagus cuniculus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 Oryctolagus cuniculus])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ALDOA_RABIT ALDOA_RABIT]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.<ref>PMID:17329259</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Fructose-bisphosphate aldolase]]		[[Category: Fructose-bisphosphate aldolase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]
	[[Category: St-Jean, M.]]		[[Category: St-Jean, M]]
	[[Category: Sygusch, J.]]		[[Category: Sygusch, J]]
	[[Category: Aldolase]]		[[Category: Aldolase]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]

OCA at 13:32, 29 September 2014

2014-09-29T13:32:21Z

← Older revision		Revision as of 13:32, 29 September 2014
Line 1:		Line 1:
	[[Image:~~3dft~~.~~png~~\|~~left~~\|~~200px~~]]		==Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle==
			<StructureSection load='3dft' size='340' side='right' caption='[[3dft]], [[Resolution\|resolution]] 1.94Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DFT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dfn\|3dfn]], [[3dfo\|3dfo]], [[3dfp\|3dfp]], [[3dfq\|3dfq]], [[3dfs\|3dfs]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 Oryctolagus cuniculus])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dft OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dft RCSB], [http://www.ebi.ac.uk/pdbsum/3dft PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/df/3dft_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 and Ser-33 mutants were examined by kinetic and structural analyses. The mutations significantly compromised enzymatic activity and carbanion oxidation in presence of DHAP. Detailed structural analysis demonstrated that, like native crystals, Asp-33 mutant crystals, soaked in DHAP solutions, trapped Schiff base-derived intermediates covalently attached to Lys-229. The mutant structures, however, exhibited an abridged conformational change with the helical region (34-65) flanking the active site as well as pK(a) reductions and increased side chain disorder by central lysine residues, Lys-107 and Lys-146. These changes directly affect their interaction with the C-terminal Tyr-363, consistent with the absence of active site binding by the C-terminal region in the presence of phosphate. Lys-146 pK(a) reduction and side chain disorder would further compromise charge stabilization during C-C bond cleavage and proton transfer during enamine formation. These mechanistic impediments explain diminished catalytic activity and a reduced level of carbanion oxidation and are consistent with rate-determining proton transfer observed in the Asn-33 mutant. Asp-33 reduces the entropic cost and augments the enthalpic gain during catalysis by rigidifying Lys-107 and Lys-146, stabilizing their protonated forms, and promoting a conformational change triggered by substrate or obligate product binding, which lower kinetic barriers in C-C bond cleavage and Schiff base-enamine interconversion.

	~~{{STRUCTURE_3dft\| PDB=3dft \| SCENE= }}~~		Charge Stabilization and Entropy Reduction of Central Lysine Residues in Fructose-Bisphosphate Aldolase.,St-Jean M, Blonski C, Sygusch J Biochemistry. 2009 Apr 22. PMID:19354220<ref>PMID:19354220</ref>

	~~===Phosphate ions in D33S mutant fructose-1~~,~~6-bisphosphate aldolase from rabbit muscle===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19354220}}~~

	~~==About this Structure==~~
	~~[[3dft]] is~~ a ~~4 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=3DFT OCA].~~

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019354220<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Fructose-bisphosphate aldolase]]		[[Category: Fructose-bisphosphate aldolase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]

OCA at 14:53, 20 October 2012

2012-10-20T14:53:19Z

← Older revision		Revision as of 14:53, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3dft]] is a 4 chain structure ~~of [[Aldolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA].		[[3dft]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA].

	==See Also==		==See Also==

OCA: Protected "3dft" [edit=sysop:move=sysop]

2012-07-26T16:36:11Z

Protected "3dft" [edit=sysop:move=sysop]

← Older revision	Revision as of 16:36, 26 July 2012
(No difference)

OCA at 16:36, 26 July 2012

2012-07-26T16:36:09Z

← Older revision		Revision as of 16:36, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3dft.png\|left\|200px]]		[[Image:3dft.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3dft", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3dft\| PDB=3dft \| SCENE= }}		{{STRUCTURE_3dft\| PDB=3dft \| SCENE= }}

	===Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle===		===Phosphate ions in D33S mutant fructose-1,6-bisphosphate aldolase from rabbit muscle===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19354220}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19354220 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19354220}}		{{ABSTRACT_PUBMED_19354220}}

	==About this Structure==		==About this Structure==
	~~3DFT~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA].		[[3dft]] is a 4 chain structure of [[Aldolase]] with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DFT OCA].

			==See Also==
			*[[Aldolase\|Aldolase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19354220~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019354220</ref><references group="xtra"/>
	[[Category: Fructose-bisphosphate aldolase]]		[[Category: Fructose-bisphosphate aldolase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]
	[[Category: St-Jean, M.]]		[[Category: St-Jean, M.]]
	[[Category: Sygusch, J.]]		[[Category: Sygusch, J.]]
	~~[[Category: Acetylation]]~~
	[[Category: Aldolase]]		[[Category: Aldolase]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
Line 37:		Line 27:
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
	[[Category: Schiff base]]		[[Category: Schiff base]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:48:52 2009''~~

OCA at 07:48, 6 May 2009

2009-05-06T07:48:53Z

← Older revision		Revision as of 07:48, 6 May 2009
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	{{Seed}}		{{Seed}}
	[[Image:3dft.~~jpg~~\|left\|200px]]		[[Image:3dft.png\|left\|200px]]

	<!--		<!--
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	[[Category: Schiff base]]		[[Category: Schiff base]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Apr 30 09~~:46:42 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:48:52 2009''