3doi - Revision history

OCA at 08:24, 20 March 2024

2024-03-20T08:24:14Z

← Older revision		Revision as of 08:24, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3doi' size='340' side='right'caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3doi' size='340' side='right'caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DOI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DOI FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[3doh\|3doh]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>TM_0033 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~2336 ATCC 43589])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [https://pdbe.org/3doi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [https://www.ebi.ac.uk/pdbsum/3doi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3doi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [https://pdbe.org/3doi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [https://www.ebi.ac.uk/pdbsum/3doi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3doi ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q9WXP0_THEMA Q9WXP0_THEMA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3doi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3doi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Comparative analysis of the genome of the hyperthermophilic bacterium Thermotoga maritima revealed a hypothetical protein (EstA) with typical esterase features. The EstA protein was functionally produced in Escherichia coli and purified to homogeneity. It indeed displayed esterase activity with optima at or above 95 degrees C and at pH 8.5, with a preference for esters with short acyl chains (C2-C10). Its 2.6-A-resolution crystal structure revealed a classical alpha/beta hydrolase domain with a catalytic triad consisting of a serine, an aspartate, and a histidine. EstA is irreversibly inhibited by the organophosphate paraoxon. A 3.0-A-resolution structure confirmed that this inhibitor binds covalently to the catalytic serine residue of EstA. Remarkably, the structure also revealed the presence of an N-terminal immunoglobulin (Ig)-like domain, which is unprecedented among esterases. EstA forms a hexamer both in the crystal and in solution. Electron microscopy showed that the hexamer in solution is identical with the hexamer in the crystal, which is formed by two trimers, with the N-terminal domains facing each other. Mutational studies confirmed that residues Phe89, Phe112, Phe116, Phe246, and Trp377 affect enzyme activity. A truncated mutant of EstA, in which the Ig-like domain was removed, showed only 5% of wild-type activity, had lower thermostability, and failed to form hexamers. These data suggest that the Ig-like domain plays an important role in the enzyme multimerization and activity of EstA.

	Crystal structure and biochemical properties of a novel thermostable esterase containing an immunoglobulin-like domain.,Levisson M, Sun L, Hendriks S, Swinkels P, Akveld T, Bultema JB, Barendregt A, van den Heuvel RH, Dijkstra BW, van der Oost J, Kengen SW J Mol Biol. 2009 Jan 23;385(3):949-62. Epub 2008 Nov 5. PMID:19013466<ref>PMID:19013466</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3doi" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 43589]]~~
	~~[[Category: Carboxylesterase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Dijkstra, B W~~]]		[[Category: Thermotoga maritima]]
	[[Category: ~~Hendriks, S~~]]		[[Category: Dijkstra BW]]
	[[Category: ~~Kengen,~~ S ~~W.M~~]]		[[Category: Hendriks S]]
	[[Category: ~~Levisson, M~~]]		[[Category: Kengen SWM]]
	[[Category: ~~Oost, J Van der~~]]		[[Category: Levisson M]]
	[[Category: Sun, L]]		[[Category: Sun L]]
	[[Category: ~~Alpha-beta hydrolase]]~~		[[Category: Van der Oost J]]
	~~[[Category: Beta sheet]]~~
	~~[[Category: Hydrolase~~]]

OCA at 08:04, 9 February 2022

2022-02-09T08:04:10Z

← Older revision		Revision as of 08:04, 9 February 2022
Line 1:		Line 1:

	==Crystal Structure of a Thermostable Esterase complex with paraoxon==		==Crystal Structure of a Thermostable Esterase complex with paraoxon==
	<StructureSection load='3doi' size='340' side='right' caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3doi' size='340' side='right'caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3DOI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DOI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3doh\|3doh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3doh\|3doh]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0033 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0033 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [~~http~~://pdbe.org/3doi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3doi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3doi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [https://pdbe.org/3doi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [https://www.ebi.ac.uk/pdbsum/3doi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3doi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 35:		Line 35:
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
			[[Category: Large Structures]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Hendriks, S]]		[[Category: Hendriks, S]]

OCA at 09:44, 14 November 2018

2018-11-14T09:44:51Z

← Older revision		Revision as of 09:44, 14 November 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/3doi_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/3doi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:12, 11 August 2016

2016-08-11T19:12:47Z

← Older revision		Revision as of 19:12, 11 August 2016
Line 1:		Line 1:

	==Crystal Structure of a Thermostable Esterase complex with paraoxon==		==Crystal Structure of a Thermostable Esterase complex with paraoxon==
	<StructureSection load='3doi' size='340' side='right' caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3doi' size='340' side='right' caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DOI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DOI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3doh\|3doh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3doh\|3doh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0033 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ~~Thermotoga maritima~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0033 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [http://www.ebi.ac.uk/pdbsum/3doi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [http://pdbe.org/3doi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [http://www.ebi.ac.uk/pdbsum/3doi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3doi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3doi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3doi" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 43589]]
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	~~[[Category: Thermotoga maritima]]~~
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Hendriks, S]]		[[Category: Hendriks, S]]

OCA at 08:01, 12 November 2014

2014-11-12T08:01:08Z

← Older revision		Revision as of 08:01, 12 November 2014
Line 1:		Line 1:
	[[Image:~~3doi~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of a Thermostable Esterase complex with paraoxon==
			<StructureSection load='3doi' size='340' side='right' caption='[[3doi]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3doi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DOI FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3doh\|3doh]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0033 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3doi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3doi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3doi RCSB], [http://www.ebi.ac.uk/pdbsum/3doi PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/3doi_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Comparative analysis of the genome of the hyperthermophilic bacterium Thermotoga maritima revealed a hypothetical protein (EstA) with typical esterase features. The EstA protein was functionally produced in Escherichia coli and purified to homogeneity. It indeed displayed esterase activity with optima at or above 95 degrees C and at pH 8.5, with a preference for esters with short acyl chains (C2-C10). Its 2.6-A-resolution crystal structure revealed a classical alpha/beta hydrolase domain with a catalytic triad consisting of a serine, an aspartate, and a histidine. EstA is irreversibly inhibited by the organophosphate paraoxon. A 3.0-A-resolution structure confirmed that this inhibitor binds covalently to the catalytic serine residue of EstA. Remarkably, the structure also revealed the presence of an N-terminal immunoglobulin (Ig)-like domain, which is unprecedented among esterases. EstA forms a hexamer both in the crystal and in solution. Electron microscopy showed that the hexamer in solution is identical with the hexamer in the crystal, which is formed by two trimers, with the N-terminal domains facing each other. Mutational studies confirmed that residues Phe89, Phe112, Phe116, Phe246, and Trp377 affect enzyme activity. A truncated mutant of EstA, in which the Ig-like domain was removed, showed only 5% of wild-type activity, had lower thermostability, and failed to form hexamers. These data suggest that the Ig-like domain plays an important role in the enzyme multimerization and activity of EstA.

	~~{{STRUCTURE_3doi\| PDB=3doi \| SCENE= }}~~		Crystal structure and biochemical properties of a novel thermostable esterase containing an immunoglobulin-like domain.,Levisson M, Sun L, Hendriks S, Swinkels P, Akveld T, Bultema JB, Barendregt A, van den Heuvel RH, Dijkstra BW, van der Oost J, Kengen SW J Mol Biol. 2009 Jan 23;385(3):949-62. Epub 2008 Nov 5. PMID:19013466<ref>PMID:19013466</ref>

	~~===Crystal Structure~~ of ~~a Thermostable Esterase complex with paraoxon===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19013466}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3doi]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019013466<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Dijkstra, B W.]]		[[Category: Dijkstra, B W]]
	[[Category: Hendriks, S.]]		[[Category: Hendriks, S]]
	[[Category: Kengen, S W.M.]]		[[Category: Kengen, S W.M]]
	[[Category: Levisson, M.]]		[[Category: Levisson, M]]
	[[Category: Oost, J Van der.]]		[[Category: Oost, J Van der]]
	[[Category: Sun, L.]]		[[Category: Sun, L]]
	[[Category: Alpha-beta hydrolase]]		[[Category: Alpha-beta hydrolase]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA: Protected "3doi" [edit=sysop:move=sysop]

2013-01-07T21:35:59Z

Protected "3doi" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:35, 7 January 2013
(No difference)

OCA at 21:35, 7 January 2013

2013-01-07T21:35:55Z

← Older revision		Revision as of 21:35, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:3doi.png\|left\|200px]]
	[[Image:3doi.~~jpg~~\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3doi", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3doi\| PDB=3doi \| SCENE= }}		{{STRUCTURE_3doi\| PDB=3doi \| SCENE= }}

	===Crystal Structure of a Thermostable Esterase complex with paraoxon===		===Crystal Structure of a Thermostable Esterase complex with paraoxon===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19013466}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19013466 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19013466}}		{{ABSTRACT_PUBMED_19013466}}

	==About this Structure==		==About this Structure==
	~~3DOI~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA].		[[3doi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DOI OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19013466~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019013466</ref><references group="xtra"/>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
Line 34:		Line 22:
	[[Category: Alpha-beta hydrolase]]		[[Category: Alpha-beta hydrolase]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
			[[Category: Hydrolase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 09:04:17 2009''~~

OCA at 07:04, 18 February 2009

2009-02-18T07:04:17Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3doi is ON HOLD  until Paper Publication
+<!--
-Authors: Levisson, M., Sun, L., Hendriks, S., Swinkels, P., Akveld, T., Bultema, J.B., Barendregt, A., van den Heuvel, R.H.H., Dijkstra, B.W., van der Oost, J., Kengen, S.W.M.
+===Crystal Structure of a Thermostable Esterase complex with paraoxon===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 12:15:51 2008''
+<!--

OCA: New page: '''Unreleased structure''' The entry 3doi is ON HOLD until Paper Publication Authors: Levisson, M., Sun, L., Hendriks, S., Swinkels, P., Akveld, T., Bultema, J.B., Barendregt, A., van d...

2008-07-23T09:15:51Z

New page: '''Unreleased structure''' The entry 3doi is ON HOLD until Paper Publication Authors: Levisson, M., Sun, L., Hendriks, S., Swinkels, P., Akveld, T., Bultema, J.B., Barendregt, A., van d...

New page

'''Unreleased structure'''

The entry 3doi is ON HOLD until Paper Publication

Authors: Levisson, M., Sun, L., Hendriks, S., Swinkels, P., Akveld, T., Bultema, J.B., Barendregt, A., van den Heuvel, R.H.H., Dijkstra, B.W., van der Oost, J., Kengen, S.W.M.

Description: Crystal Structure of a Thermostable Esterase complex with paraoxon

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 12:15:51 2008''