3dpc - Revision history

OCA at 15:13, 1 November 2023

2023-11-01T15:13:14Z

← Older revision		Revision as of 15:13, 1 November 2023
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	<StructureSection load='3dpc' size='340' side='right'caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3dpc' size='340' side='right'caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dpc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dpc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DPC FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~, ~~<scene name='pdbligand=TRS:~~2~~-AMINO-2-HYDROXYMETHYL-PROPANE-1,~~3~~-DIOL'>TRS</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~TPO~~:~~PHOSPHOTHREONINE~~'>~~TPO~~</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~phoA, b0383~~, ~~JW0374 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~<~~/td></tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.~~1.3~~.1 3.1.3.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [https://pdbe.org/3dpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [https://www.ebi.ac.uk/pdbsum/3dpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [https://pdbe.org/3dpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [https://www.ebi.ac.uk/pdbsum/3dpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpc ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PPB_ECOLI PPB_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Alkaline phosphatase]]~~		[[Category: Escherichia coli K-12]]
	~~[[Category: Ecoli~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jiang, T]]		[[Category: Jiang T]]
	[[Category: Wang~~, W H]]~~		[[Category: Wang WH]]
	~~[[Category: Complex structure]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Magnesium]]~~
	~~[[Category: Metal-binding]]~~
	~~[[Category: Phosphoprotein]]~~
	~~[[Category: Protein kinase~~]]

OCA at 07:33, 10 November 2021

2021-11-10T07:33:31Z

← Older revision		Revision as of 07:33, 10 November 2021
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	==Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide==		==Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide==
	<StructureSection load='3dpc' size='340' side='right' caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3dpc' size='340' side='right'caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3dpc]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3DPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3dpc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DPC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [~~http~~://pdbe.org/3dpc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3dpc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [https://pdbe.org/3dpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [https://www.ebi.ac.uk/pdbsum/3dpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Alkaline phosphatase\|Alkaline phosphatase]]		*[[Alkaline phosphatase 3D structures\|Alkaline phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
			[[Category: Large Structures]]
	[[Category: Jiang, T]]		[[Category: Jiang, T]]
	[[Category: Wang, W H]]		[[Category: Wang, W H]]

OCA at 09:24, 14 November 2018

2018-11-14T09:24:25Z

← Older revision		Revision as of 09:24, 14 November 2018
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	==Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide==		==Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide==
	<StructureSection load='3dpc' size='340' side='right' caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3dpc' size='340' side='right' caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [http://pdbe.org/3dpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [http://www.ebi.ac.uk/pdbsum/3dpc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [http://pdbe.org/3dpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [http://www.ebi.ac.uk/pdbsum/3dpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dpc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dpc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:29, 8 February 2016

2016-02-08T21:29:28Z

← Older revision		Revision as of 21:29, 8 February 2016
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [http://www.ebi.ac.uk/pdbsum/3dpc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [http://pdbe.org/3dpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [http://www.ebi.ac.uk/pdbsum/3dpc PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dpc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3dpc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:00, 21 December 2014

2014-12-21T18:00:27Z

← Older revision		Revision as of 18:00, 21 December 2014
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	~~{{STRUCTURE_3dpc\| PDB=3dpc \| SCENE= }}~~		==Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide==
	===Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide===		<StructureSection load='3dpc' size='340' side='right' caption='[[3dpc]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_19349157}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3dpc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DPC FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dpc RCSB], [http://www.ebi.ac.uk/pdbsum/3dpc PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dpc_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			This study describes the development of a universal phosphorylated peptide-binding protein designed to simultaneously detect serine, threonine and tyrosine kinases. The Escherichia coli alkaline phosphatase (EAP) is a well-defined nonspecific phosphated monoesterase and Ser-, Thr- or Tyr-phosphorylated peptides served as substrates for EAP in preliminary experiments. Based on the known catalytic mechanism of EAP, the recombinant site-directed mutant EAP-S102L was generated, whose catalytic activity was blocked, but its binding ability was preserved. For EAP-S102L the catalytic rate constant, k(cat), was reduced by a factor of 1000, while the Michaelis-Menten constant, K(m), remained almost unchanged. Crystallographic analysis of the EAP-S102L/phophorylated peptide complex revealed that EAP-S102L could bind the phosphate group of the phosphorylated peptide but lacked nucleophilic attack potential which was essential for the catalytic ability of EAP. Finally, by combining the fluorescence-labeled EAP-S102L with non-phophorylated peptide chips, kinases could be detected from tumor cell samples. The recombinant EAP-S102L construct is perhaps the first functional binding protein derived from a native enzyme, illustrating how one single mutation tremendously alters protein function.

	~~==About this Structure==~~		Development of a universal phosphorylated peptide-binding protein for simultaneous assay of kinases.,Li W, Bi L, Wang W, Li Y, Zhou Y, Wei H, Jiang T, Bai L, Chen Y, Zhang Z, Yuan X, Xiao J, Zhang XE Biosens Bioelectron. 2009 May 15;24(9):2871-7. Epub 2009 Mar 3. PMID:19349157<ref>PMID:19349157</ref>
	~~[[3dpc]] is~~ a ~~3 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3DPC OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Alkaline phosphatase\|Alkaline phosphatase]]		*[[Alkaline phosphatase\|Alkaline phosphatase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:019349157</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
	[[Category: Jiang, T.]]		[[Category: Jiang, T]]
	[[Category: Wang, W H~~.]]~~		[[Category: Wang, W H]]
	~~[[Category: Alkaline phosphatase~~]]
	[[Category: Complex structure]]		[[Category: Complex structure]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:30, 5 February 2014

2014-02-05T08:30:52Z

← Older revision		Revision as of 08:30, 5 February 2014
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	~~[[Image:3dpc.png\|left\|200px]]~~

	{{STRUCTURE_3dpc\| PDB=3dpc \| SCENE= }}		{{STRUCTURE_3dpc\| PDB=3dpc \| SCENE= }}

	===Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide===		===Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide===

	{{ABSTRACT_PUBMED_19349157}}		{{ABSTRACT_PUBMED_19349157}}

	==About this Structure==		==About this Structure==
	[[3dpc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].		[[3dpc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].

	==See Also==		==See Also==
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	==Reference==		==Reference==
	<ref group="xtra">PMID:019349157</ref><references group="xtra"/>		<ref group="xtra">PMID:019349157</ref><references group="xtra"/><references/>
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: ~~Escherichia coli~~]]		[[Category: Ecoli]]
	[[Category: Jiang, T.]]		[[Category: Jiang, T.]]
	[[Category: Wang, W H.]]		[[Category: Wang, W H.]]

OCA at 11:32, 20 October 2012

2012-10-20T11:32:17Z

← Older revision		Revision as of 11:32, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3dpc]] is a 3 chain structure ~~of [[Alkaline phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].		[[3dpc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].

	==See Also==		==See Also==

OCA: Protected "3dpc" [edit=sysop:move=sysop]

2012-07-26T05:43:11Z

Protected "3dpc" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:43, 26 July 2012
(No difference)

OCA at 05:43, 26 July 2012

2012-07-26T05:43:09Z

← Older revision		Revision as of 05:43, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3dpc.png\|left\|200px]]		[[Image:3dpc.png\|left\|200px]]

	~~<!--~~
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	{{STRUCTURE_3dpc\| PDB=3dpc \| SCENE= }}		{{STRUCTURE_3dpc\| PDB=3dpc \| SCENE= }}

	===Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide===		===Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19349157}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_19349157}}		{{ABSTRACT_PUBMED_19349157}}

	==About this Structure==		==About this Structure==
	~~3DPC~~ is a 3 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k12~~ Escherichia coli ~~k12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].		[[3dpc]] is a 3 chain structure of [[Alkaline phosphatase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPC OCA].

			==See Also==
			*[[Alkaline phosphatase\|Alkaline phosphatase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19349157~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019349157</ref><references group="xtra"/>
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Escherichia coli ~~k12~~]]		[[Category: Escherichia coli]]
	[[Category: Jiang, T.]]		[[Category: Jiang, T.]]
	[[Category: Wang, W H.]]		[[Category: Wang, W H.]]
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	[[Category: Magnesium]]		[[Category: Magnesium]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	~~[[Category: Periplasm]]~~
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
	[[Category: Protein kinase]]		[[Category: Protein kinase]]
	~~[[Category: Zinc]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:30:58 2009''~~

OCA at 05:31, 25 June 2009

2009-06-25T05:31:00Z

← Older revision		Revision as of 05:31, 25 June 2009
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	{{Seed}}		{{Seed}}
	[[Image:3dpc.~~jpg~~\|left\|200px]]		[[Image:3dpc.png\|left\|200px]]

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	[[Category: Zinc]]		[[Category: Zinc]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 10~~:25:32 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:30:58 2009''