3e2a - Revision history

OCA at 12:57, 30 August 2023

2023-08-30T12:57:13Z

← Older revision		Revision as of 12:57, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='3e2a' size='340' side='right'caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3e2a' size='340' side='right'caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium~~ influenzae~~" lehmann and neumann 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2A FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.301Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>can, HI1301 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~727~~ "~~Bacterium influenzae~~" ~~Lehmann and Neumann 1896])~~<~~/td~~></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [https://pdbe.org/3e2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [https://www.ebi.ac.uk/pdbsum/3e2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [https://pdbe.org/3e2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [https://www.ebi.ac.uk/pdbsum/3e2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2a ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CAN_HAEIN CAN_HAEIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacterium~~ influenzae ~~lehmann and neumann 1896]]~~		[[Category: Haemophilus influenzae]]
	~~[[Category: Carbonate dehydratase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lee, J]]		[[Category: Lee J]]
	[[Category: Rowlett~~, R S]]~~		[[Category: Rowlett RS]]
	~~[[Category: Allosteric site mutant]]~~
	~~[[Category: Beta carbonic anhydrase]]~~
	~~[[Category: Lyase]]~~
	~~[[Category: Metal-binding~~]]

OCA at 19:33, 20 October 2021

2021-10-20T19:33:24Z

← Older revision		Revision as of 19:33, 20 October 2021
Line 1:		Line 1:

	==H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate==		==H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate==
	<StructureSection load='3e2a' size='340' side='right' caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3e2a' size='340' side='right'caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3E2A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [~~http~~://pdbe.org/3e2a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3e2a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [https://pdbe.org/3e2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [https://www.ebi.ac.uk/pdbsum/3e2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2a_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3e2a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3e2a" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Bacterium influenzae lehmann and neumann 1896]]		[[Category: Bacterium influenzae lehmann and neumann 1896]]
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
			[[Category: Large Structures]]
	[[Category: Lee, J]]		[[Category: Lee, J]]
	[[Category: Rowlett, R S]]		[[Category: Rowlett, R S]]

OCA at 09:00, 25 October 2017

2017-10-25T09:00:45Z

← Older revision		Revision as of 09:00, 25 October 2017
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 3e2a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3e2a" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Carbonic anhydrase\|Carbonic anhydrase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:28, 11 August 2016

2016-08-11T11:28:30Z

← Older revision		Revision as of 11:28, 11 August 2016
Line 1:		Line 1:

	==H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate==		==H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate==
	<StructureSection load='3e2a' size='340' side='right' caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3e2a' size='340' side='right' caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Haemophilus_influenzae Haemophilus~~ influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E2A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E2A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 ~~Haemophilus~~ influenzae])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [http://www.ebi.ac.uk/pdbsum/3e2a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [http://pdbe.org/3e2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [http://www.ebi.ac.uk/pdbsum/3e2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e2a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3e2a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacterium influenzae lehmann and neumann 1896]]
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	~~[[Category: Haemophilus influenzae]]~~
	[[Category: Lee, J]]		[[Category: Lee, J]]
	[[Category: Rowlett, R S]]		[[Category: Rowlett, R S]]

OCA at 08:47, 20 January 2015

2015-01-20T08:47:35Z

← Older revision		Revision as of 08:47, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E2A FirstGlance]. <br>		<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E2A FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [http://www.ebi.ac.uk/pdbsum/3e2a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [http://www.ebi.ac.uk/pdbsum/3e2a PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
	[[Category: Lee, J.]]		[[Category: Lee, J]]
	[[Category: Rowlett, R S.]]		[[Category: Rowlett, R S]]
	[[Category: Allosteric site mutant]]		[[Category: Allosteric site mutant]]
	[[Category: Beta carbonic anhydrase]]		[[Category: Beta carbonic anhydrase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]

OCA at 11:24, 29 September 2014

2014-09-29T11:24:06Z

← Older revision		Revision as of 11:24, 29 September 2014
Line 1:		Line 1:
	[[Image:~~3e2a~~.~~png~~\|~~left~~\|~~200px~~]]		==H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate==
			<StructureSection load='3e2a' size='340' side='right' caption='[[3e2a]], [[Resolution\|resolution]] 2.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E2A FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8c\|2a8c]], [[2a8d\|2a8d]], [[3e24\|3e24]], [[3e28\|3e28]], [[3e2w\|3e2w]], [[3e2x\|3e2x]], [[3e31\|3e31]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">can, HI1301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e2a RCSB], [http://www.ebi.ac.uk/pdbsum/3e2a PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2a_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Haemophilus influenzae beta-carbonic anhydrase (HICA) is hypothesized to be an allosteric protein that is regulated by the binding of bicarbonate ion to a non-catalytic (inhibitory) site that controls the ligation of Asp44 to the catalytically essential zinc ion. We report here the X-ray crystallographic structures of two variants (W39F and Y181F) involved in the binding of bicarbonate ion in the non-catalytic site and an active site variant (D44N) that is incapable of forming a strong zinc ligand. The mutation of Trp39 to Phe increases the apparent Ki for bicarbonate inhibition by 4.8-fold. While the structures of W39F and Y181F are very similar to the wild-type enzyme, the X-ray crystal structure of the D44N variant reveals that it has adopted an active site conformation nearly identical to that of non-allosteric beta-carbonic anhydrases. We propose that the structure of the D44N variant is likely to be representative of the active conformation of the enzyme. These results lend additional support to the hypothesis that HICA is an allosteric enzyme that can adopt active and inactive conformations, the latter of which is stabilized by bicarbonate ion binding to a non-catalytic site.

	~~{{STRUCTURE_3e2a\| PDB=3e2a \| SCENE= }}~~		Allosteric Site Variants of Haemophilus influenzae beta-Carbonic Anhydrase.,Rowlett RS, Tu C, Lee J, Herman AG, Chapnick DA, Shah SH, Gareiss PC Biochemistry. 2009 May 21. PMID:19459702<ref>PMID:19459702</ref>

	~~===H. influenzae beta-carbonic anhydrase~~, ~~variant Y181F with 100 mM bicarbonate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19459702}}~~

	~~==About this Structure==~~
	~~[[3e2a]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Haemophilus_influenzae Haemophilus influenzae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA].~~

	==See Also==		==See Also==
	*[[Carbonic anhydrase\|Carbonic anhydrase]]		*[[Carbonic anhydrase\|Carbonic anhydrase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019459702<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]

OCA at 19:30, 20 October 2012

2012-10-20T19:30:23Z

← Older revision		Revision as of 19:30, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3e2a]] is a 6 chain structure ~~of [[Carbonic anhydrase]]~~ with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA].		[[3e2a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA].

	==See Also==		==See Also==

OCA: Protected "3e2a" [edit=sysop:move=sysop]

2012-07-27T07:34:53Z

Protected "3e2a" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:34, 27 July 2012
(No difference)

OCA at 07:34, 27 July 2012

2012-07-27T07:34:51Z

← Older revision		Revision as of 07:34, 27 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:3e2a.png\|left\|200px]]
	[[Image:3e2a.~~jpg~~\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3e2a", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3e2a\| PDB=3e2a \| SCENE= }}		{{STRUCTURE_3e2a\| PDB=3e2a \| SCENE= }}

	===H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate===		===H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19459702}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19459702 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19459702}}		{{ABSTRACT_PUBMED_19459702}}

	==About this Structure==		==About this Structure==
	~~3E2A~~ is a 6 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA].		[[3e2a]] is a 6 chain structure of [[Carbonic anhydrase]] with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2A OCA].

			==See Also==
			*[[Carbonic anhydrase\|Carbonic anhydrase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19459702~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019459702</ref><references group="xtra"/>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
Line 32:		Line 23:
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	~~[[Category: Zinc]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 4 07:28:20 2009''~~

OCA at 04:28, 4 June 2009

2009-06-04T04:28:21Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3e2a is ON HOLD  until Paper Publication
+<!--
-Authors: Rowlett, R.S., Lee, J.
+===H. influenzae beta-carbonic anhydrase, variant Y181F with 100 mM bicarbonate===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 27 10:59:46 2008''
+<!--