3eba - Revision history

OCA at 09:49, 6 November 2024

2024-11-06T09:49:43Z

← Older revision		Revision as of 09:49, 6 November 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/3eba_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/3eba_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 15:20, 1 November 2023

2023-11-01T15:20:27Z

← Older revision		Revision as of 15:20, 1 November 2023
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	<StructureSection load='3eba' size='340' side='right'caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3eba' size='340' side='right'caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arabian_camel Arabian camel~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3EBA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EBA FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1op9\|1op9]]</td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]~~ </~~span~~></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [https://pdbe.org/3eba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [https://www.ebi.ac.uk/pdbsum/3eba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eba ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [~~http~~://pdbe.org/3eba PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3eba PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3eba ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[~~[http~~://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:[~~http~~://omim.org/entry/105200 105200]]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.<ref>PMID:8464497</ref>		[https://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN] Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:[https://omim.org/entry/105200 105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.<ref>PMID:8464497</ref>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.		[https://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arabian camel~~]]		[[Category: Camelus dromedarius]]
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Lysozyme]]~~		[[Category: Conrath K]]
	[[Category: Conrath, K]]		[[Category: Loris R]]
	[[Category: Loris, R]]		[[Category: Martinez-Rodriguez S]]
	[[Category: Martinez-Rodriguez, S]]		[[Category: Muyldermans S]]
	[[Category: Muyldermans, S]]		[[Category: Saerens D]]
	[[Category: Saerens, D]]		[[Category: Vincke C]]
	[[Category: Vincke, C]]
	~~[[Category: Amyloid]]~~
	~~[[Category: Amyloid fibril formation inhibition]]~~
	~~[[Category: Antigen-antibody complex]]~~
	~~[[Category: Antimicrobial]]~~
	~~[[Category: Bacteriolytic enzyme]]~~
	~~[[Category: Disease mutation]]~~
	~~[[Category: Glycosidase]]~~
	~~[[Category: Humanization]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Immune system-hydrolase complex]]~~
	~~[[Category: Immunoglobulin]]~~
	~~[[Category: Nanobody]]~~
	~~[[Category: Polymorphism~~]]

OCA at 10:21, 9 September 2020

2020-09-09T10:21:36Z

← Older revision		Revision as of 10:21, 9 September 2020
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	==CAbHul6 FGLW mutant (humanized) in complex with human lysozyme==		==CAbHul6 FGLW mutant (humanized) in complex with human lysozyme==
	<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3eba' size='340' side='right'caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EBA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3EBA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://pdbe.org/3eba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eba ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://pdbe.org/3eba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eba ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	[[Category: Arabian camel]]		[[Category: Arabian camel]]
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Conrath, K]]		[[Category: Conrath, K]]

OCA at 21:52, 2 December 2018

2018-12-02T21:52:03Z

← Older revision		Revision as of 21:52, 2 December 2018
Line 1:		Line 1:

	==CAbHul6 FGLW mutant (humanized) in complex with human lysozyme==		==CAbHul6 FGLW mutant (humanized) in complex with human lysozyme==
	<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://pdbe.org/3eba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://pdbe.org/3eba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eba ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/3eba_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/3eba_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Nanobody]]		[[Category: Nanobody]]
			[[Category: Polymorphism]]

OCA at 06:22, 9 February 2016

2016-02-09T06:22:21Z

← Older revision		Revision as of 06:22, 9 February 2016
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	<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Camelus_dromedarius Camelus dromedarius~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EBA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EBA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://pdbe.org/3eba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eba ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3eba" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Camelus dromedarius~~]]		[[Category: Arabian camel]]
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Conrath, K]]		[[Category: Conrath, K]]

OCA at 13:55, 18 December 2014

2014-12-18T13:55:12Z

← Older revision		Revision as of 13:55, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3eba\| PDB=3eba \| SCENE= }}~~		==CAbHul6 FGLW mutant (humanized) in complex with human lysozyme==
	===CAbHul6 FGLW mutant (humanized) in complex with human lysozyme===		<StructureSection load='3eba' size='340' side='right' caption='[[3eba]], [[Resolution\|resolution]] 1.85Å' scene=''>
	~~{{ABSTRACT_PUBMED_19010777}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EBA FirstGlance]. <br>
	==Disease==		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	[[http://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:[http://omim.org/entry/105200 105200]]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.<ref>PMID:8464497</ref>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1op9\|1op9]]</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eba OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eba RCSB], [http://www.ebi.ac.uk/pdbsum/3eba PDBsum]</span></td></tr>
			</table>
			== Disease ==
			[[http://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:[http://omim.org/entry/105200 105200]]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.<ref>PMID:8464497</ref>
			== Function ==
			[[http://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/3eba_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Nanobodies, single-domain antigen-binding fragments of camelid-specific heavy-chain only antibodies offer special advantages in therapy over classic antibody fragments because of their smaller size, robustness, and preference to target unique epitopes. A Nanobody differs from a human heavy chain variable domain in about ten amino acids spread all over its surface, four hallmark Nanobody-specific amino acids in the framework-2 region (positions 42, 49, 50, and 52), and a longer third antigen-binding loop (H3) folding over this area. For therapeutic applications the camelid-specific amino acid sequences in the framework have to be mutated to their human heavy chain variable domain equivalent, i.e. humanized. We performed this humanization exercise with Nanobodies of the subfamily that represents close to 80% of all dromedary-derived Nanobodies and investigated the effects on antigen affinity, solubility, expression yield, and stability. It is demonstrated that the humanization of Nanobody-specific residues outside framework-2 are neutral to the Nanobody properties. Surprisingly, the Glu-49 --> Gly and Arg-50 --> Leu humanization of hallmark amino acids generates a single domain that is more stable though probably less soluble. The other framework-2 substitutions, Phe-42 --> Val and Gly/Ala-52 --> Trp, are detrimental for antigen affinity, due to a repositioning of the H3 loop as shown by their crystal structures. These insights were used to identify a soluble, stable, well expressed universal humanized Nanobody scaffold that allows grafts of antigen-binding loops from other Nanobodies with transfer of the antigen specificity and affinity.

	~~==Function==~~		General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.,Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K J Biol Chem. 2009 Jan 30;284(5):3273-84. Epub 2008 Nov 14. PMID:19010777<ref>PMID:19010777</ref>
	~~[[http://www~~.~~uniprot~~.~~org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Lysozymes have primarily a bacteriolytic function~~; ~~those in tissues and body fluids are associated with the monocyte~~-~~macrophage system and enhance the activity of immunoagents~~.

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[3eba]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3EBA OCA].~~		</div>

	==See Also==		==See Also==
	*[[~~Hen Egg-White (HEW)~~ Lysozyme\|~~Hen Egg-White (HEW)~~ Lysozyme]]		*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:019010777</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Conrath, K.]]		[[Category: Conrath, K]]
	[[Category: Loris, R.]]		[[Category: Loris, R]]
	[[Category: Martinez-Rodriguez, S.]]		[[Category: Martinez-Rodriguez, S]]
	[[Category: Muyldermans, S.]]		[[Category: Muyldermans, S]]
	[[Category: Saerens, D.]]		[[Category: Saerens, D]]
	[[Category: Vincke, C.]]		[[Category: Vincke, C]]
	[[Category: Amyloid]]		[[Category: Amyloid]]
	[[Category: Amyloid fibril formation inhibition]]		[[Category: Amyloid fibril formation inhibition]]

OCA at 03:44, 25 March 2013

2013-03-25T03:44:46Z

← Older revision		Revision as of 03:44, 25 March 2013
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	~~[[Image:3eba.png\|left\|200px]]~~

	{{STRUCTURE_3eba\| PDB=3eba \| SCENE= }}		{{STRUCTURE_3eba\| PDB=3eba \| SCENE= }}
			===CAbHul6 FGLW mutant (humanized) in complex with human lysozyme===
			{{ABSTRACT_PUBMED_19010777}}

	===~~CAbHul6 FGLW mutant~~ (~~humanized~~) in ~~complex with human lysozyme===~~		==Disease==
			[[http://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:[http://omim.org/entry/105200 105200]]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.<ref>PMID:8464497</ref>

	~~{{ABSTRACT_PUBMED_19010777}}~~		==Function==
			[[http://www.uniprot.org/uniprot/LYSC_HUMAN LYSC_HUMAN]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

	==About this Structure==		==About this Structure==
Line 14:		Line 16:

	==Reference==		==Reference==
	<ref group="xtra">PMID:019010777</ref><references group="xtra"/>		<ref group="xtra">PMID:019010777</ref><references group="xtra"/><references/>
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 12:56, 20 October 2012

2012-10-20T12:56:47Z

← Older revision		Revision as of 12:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3eba]] is a 2 chain structure ~~of [[Hen Egg-White (HEW) Lysozyme]]~~ with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA].		[[3eba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA].

	==See Also==		==See Also==

OCA: Protected "3eba" [edit=sysop:move=sysop]

2012-07-26T10:25:29Z

Protected "3eba" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:25, 26 July 2012
(No difference)

OCA at 10:25, 26 July 2012

2012-07-26T10:25:27Z

← Older revision		Revision as of 10:25, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3eba.png\|left\|200px]]		[[Image:3eba.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3eba", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3eba\| PDB=3eba \| SCENE= }}		{{STRUCTURE_3eba\| PDB=3eba \| SCENE= }}

	===CAbHul6 FGLW mutant (humanized) in complex with human lysozyme===		===CAbHul6 FGLW mutant (humanized) in complex with human lysozyme===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19010777}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19010777 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19010777}}		{{ABSTRACT_PUBMED_19010777}}

	==About this Structure==		==About this Structure==
	~~3EBA~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA].		[[3eba]] is a 2 chain structure of [[Hen Egg-White (HEW) Lysozyme]] with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBA OCA].

			==See Also==
			*[[Hen Egg-White (HEW) Lysozyme\|Hen Egg-White (HEW) Lysozyme]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19010777~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019010777</ref><references group="xtra"/>
	[[Category: Camelus dromedarius]]		[[Category: Camelus dromedarius]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
Line 42:		Line 33:
	[[Category: Humanization]]		[[Category: Humanization]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Immune system/hydrolase complex]]		[[Category: Immune system-hydrolase complex]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Nanobody]]		[[Category: Nanobody]]
	~~[[Category: Polymorphism]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 15 08:47:50 2009''~~