3ecc - Revision history

OCA at 09:12, 13 August 2026

2026-08-13T09:12:30Z

← Older revision		Revision as of 09:12, 13 August 2026
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The loading of oligomeric helicases onto replication origins marks an essential step in replisome assembly. In cells, dedicated AAA+ ATPases regulate loading, however, the mechanism by which these factors recruit and deposit helicases has remained unclear. To better understand this process, we determined the structure of the ATPase region of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution. The structure shows that DnaC is a close paralog of the bacterial replication initiator, DnaA, and unexpectedly shares an ability to form a helical assembly similar to that of ATP-bound DnaA. Complementation and ssDNA-binding assays validate the importance of homomeric DnaC interactions, while pull-down experiments show that the DnaC and DnaA AAA+ domains interact in a nucleotide-dependent manner. These findings implicate DnaC as a molecular adaptor that uses ATP-activated DnaA as a docking site for regulating the recruitment and correct spatial deposition of the DnaB helicase onto origins.

			Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.,Mott ML, Erzberger JP, Coons MM, Berger JM Cell. 2008 Nov 14;135(4):623-34. PMID:19013274<ref>PMID:19013274</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3ecc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[DnaC helicase loader\|DnaC helicase loader]]		*[[DnaC helicase loader\|DnaC helicase loader]]
	*[[Helicase 3D structures\|Helicase 3D structures]]		*[[Helicase 3D structures\|Helicase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:46, 21 February 2024

2024-02-21T09:46:48Z

← Older revision		Revision as of 09:46, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3ecc' size='340' side='right'caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3ecc' size='340' side='right'caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex~~ aeolicus~~" huber and stetter 2001~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ECC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ECC FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE-5~~-~~DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3ec2\|3ec2]]~~</~~div~~><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~dnaC, aq_910 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [https://pdbe.org/3ecc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [https://www.ebi.ac.uk/pdbsum/3ecc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [https://pdbe.org/3ecc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [https://www.ebi.ac.uk/pdbsum/3ecc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecc ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/O67056_AQUAE O67056_AQUAE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The loading of oligomeric helicases onto replication origins marks an essential step in replisome assembly. In cells, dedicated AAA+ ATPases regulate loading, however, the mechanism by which these factors recruit and deposit helicases has remained unclear. To better understand this process, we determined the structure of the ATPase region of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution. The structure shows that DnaC is a close paralog of the bacterial replication initiator, DnaA, and unexpectedly shares an ability to form a helical assembly similar to that of ATP-bound DnaA. Complementation and ssDNA-binding assays validate the importance of homomeric DnaC interactions, while pull-down experiments show that the DnaC and DnaA AAA+ domains interact in a nucleotide-dependent manner. These findings implicate DnaC as a molecular adaptor that uses ATP-activated DnaA as a docking site for regulating the recruitment and correct spatial deposition of the DnaB helicase onto origins.

	Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.,Mott ML, Erzberger JP, Coons MM, Berger JM Cell. 2008 Nov 14;135(4):623-34. PMID:19013274<ref>PMID:19013274</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3ecc" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[DnaC helicase loader\|DnaC helicase loader]]		*[[DnaC helicase loader\|DnaC helicase loader]]
	*[[Helicase 3D structures\|Helicase 3D structures]]		*[[Helicase 3D structures\|Helicase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus ~~huber and stetter 2001~~]]		[[Category: Aquifex aeolicus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Berger, J]]		[[Category: Berger J]]
	[[Category: Coons~~, M M~~]]		[[Category: Coons MM]]
	[[Category: Erzberger~~, J P~~]]		[[Category: Erzberger JP]]
	[[Category: Mott~~, M L]]~~		[[Category: Mott ML]]
	~~[[Category: Atp-binding]]~~
	~~[[Category: Helicase loader]]~~
	~~[[Category: Nucleotide-binding]]~~
	~~[[Category: Replication]]~~
	~~[[Category: Replication initiation factor~~]]

OCA at 11:46, 16 February 2022

2022-02-16T11:46:16Z

← Older revision		Revision as of 11:46, 16 February 2022
Line 1:		Line 1:

	==Crystal structure of the DnaC helicase loader in complex with ADP-BeF3==		==Crystal structure of the DnaC helicase loader in complex with ADP-BeF3==
	<StructureSection load='3ecc' size='340' side='right' caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3ecc' size='340' side='right'caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ECC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ECC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ec2\|3ec2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ec2\|3ec2]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaC, aq_910 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaC, aq_910 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [~~http~~://pdbe.org/3ecc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ecc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [https://pdbe.org/3ecc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [https://www.ebi.ac.uk/pdbsum/3ecc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:

	==See Also==		==See Also==
	*[[~~Structure of E. coli~~ DnaC helicase loader\|~~Structure of E. coli~~ DnaC helicase loader]]		*[[DnaC helicase loader\|DnaC helicase loader]]
			*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus huber and stetter 2001]]		[[Category: Aquifex aeolicus huber and stetter 2001]]
			[[Category: Large Structures]]
	[[Category: Berger, J]]		[[Category: Berger, J]]
	[[Category: Coons, M M]]		[[Category: Coons, M M]]

OCA at 09:08, 25 October 2017

2017-10-25T09:08:10Z

← Older revision		Revision as of 09:08, 25 October 2017
Line 30:		Line 30:

	==See Also==		==See Also==
	*[[DnaC helicase loader\|DnaC helicase loader]]
	*[[Helicase\|Helicase]]
	*[[Structure of E. coli DnaC helicase loader\|Structure of E. coli DnaC helicase loader]]		*[[Structure of E. coli DnaC helicase loader\|Structure of E. coli DnaC helicase loader]]
	== References ==		== References ==

OCA at 00:41, 5 August 2016

2016-08-05T00:41:57Z

← Older revision		Revision as of 00:41, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of the DnaC helicase loader in complex with ADP-BeF3==		==Crystal structure of the DnaC helicase loader in complex with ADP-BeF3==
	<StructureSection load='3ecc' size='340' side='right' caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3ecc' size='340' side='right' caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aquifex_aeolicus Aquifex~~ aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ECC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ECC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ec2\|3ec2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ec2\|3ec2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaC, aq_910 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaC, aq_910 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [http://www.ebi.ac.uk/pdbsum/3ecc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [http://pdbe.org/3ecc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [http://www.ebi.ac.uk/pdbsum/3ecc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ecc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus huber and stetter 2001]]
	[[Category: Berger, J]]		[[Category: Berger, J]]
	[[Category: Coons, M M]]		[[Category: Coons, M M]]

OCA at 13:54, 17 December 2014

2014-12-17T13:54:15Z

← Older revision		Revision as of 13:54, 17 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3ecc\| PDB=3ecc \| SCENE= }}~~		==Crystal structure of the DnaC helicase loader in complex with ADP-BeF3==
	===Crystal structure of the DnaC helicase loader in complex with ADP-BeF3===		<StructureSection load='3ecc' size='340' side='right' caption='[[3ecc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	~~{{ABSTRACT_PUBMED_19013274}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3ecc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ECC FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ec2\|3ec2]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaC, aq_910 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ecc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ecc RCSB], [http://www.ebi.ac.uk/pdbsum/3ecc PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecc_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The loading of oligomeric helicases onto replication origins marks an essential step in replisome assembly. In cells, dedicated AAA+ ATPases regulate loading, however, the mechanism by which these factors recruit and deposit helicases has remained unclear. To better understand this process, we determined the structure of the ATPase region of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution. The structure shows that DnaC is a close paralog of the bacterial replication initiator, DnaA, and unexpectedly shares an ability to form a helical assembly similar to that of ATP-bound DnaA. Complementation and ssDNA-binding assays validate the importance of homomeric DnaC interactions, while pull-down experiments show that the DnaC and DnaA AAA+ domains interact in a nucleotide-dependent manner. These findings implicate DnaC as a molecular adaptor that uses ATP-activated DnaA as a docking site for regulating the recruitment and correct spatial deposition of the DnaB helicase onto origins.

	~~==About this Structure==~~		Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.,Mott ML, Erzberger JP, Coons MM, Berger JM Cell. 2008 Nov 14;135(4):623-34. PMID:19013274<ref>PMID:19013274</ref>
	~~[[3ecc]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aquifex_aeolicus Aquifex aeolicus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=3ECC OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[DnaC helicase loader\|DnaC helicase loader]]		*[[DnaC helicase loader\|DnaC helicase loader]]
			*[[Helicase\|Helicase]]
	*[[Structure of E. coli DnaC helicase loader\|Structure of E. coli DnaC helicase loader]]		*[[Structure of E. coli DnaC helicase loader\|Structure of E. coli DnaC helicase loader]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019013274<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Berger, J.]]		[[Category: Berger, J]]
	[[Category: Coons, M M.]]		[[Category: Coons, M M]]
	[[Category: Erzberger, J P.]]		[[Category: Erzberger, J P]]
	[[Category: Mott, M L.]]		[[Category: Mott, M L]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Helicase loader]]		[[Category: Helicase loader]]

OCA at 10:32, 11 March 2013

2013-03-11T10:32:01Z

← Older revision		Revision as of 10:32, 11 March 2013
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	~~[[Image:3ecc.png\|left\|200px]]~~

	{{STRUCTURE_3ecc\| PDB=3ecc \| SCENE= }}		{{STRUCTURE_3ecc\| PDB=3ecc \| SCENE= }}

	===Crystal structure of the DnaC helicase loader in complex with ADP-BeF3===		===Crystal structure of the DnaC helicase loader in complex with ADP-BeF3===

	{{ABSTRACT_PUBMED_19013274}}		{{ABSTRACT_PUBMED_19013274}}

OCA at 17:48, 20 October 2012

2012-10-20T17:48:58Z

← Older revision		Revision as of 17:48, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3ecc]] is a 1 chain structure ~~of [[DnaC helicase loader]]~~ with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA].		[[3ecc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA].

	==See Also==		==See Also==

OCA: Protected "3ecc" [edit=sysop:move=sysop]

2012-07-27T02:04:39Z

Protected "3ecc" [edit=sysop:move=sysop]

← Older revision	Revision as of 02:04, 27 July 2012
(No difference)

OCA at 02:04, 27 July 2012

2012-07-27T02:04:38Z

← Older revision		Revision as of 02:04, 27 July 2012
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	~~{{Seed}}~~
	[[Image:3ecc.png\|left\|200px]]		[[Image:3ecc.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3ecc", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3ecc\| PDB=3ecc \| SCENE= }}		{{STRUCTURE_3ecc\| PDB=3ecc \| SCENE= }}

	===Crystal structure of the DnaC helicase loader in complex with ADP-BeF3===		===Crystal structure of the DnaC helicase loader in complex with ADP-BeF3===

			{{ABSTRACT_PUBMED_19013274}}

	==About this Structure==		==About this Structure==
	~~3ECC~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA].		[[3ecc]] is a 1 chain structure of [[DnaC helicase loader]] with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECC OCA].

			==See Also==
			*[[DnaC helicase loader\|DnaC helicase loader]]
			*[[Structure of E. coli DnaC helicase loader\|Structure of E. coli DnaC helicase loader]]

			==Reference==
			<ref group="xtra">PMID:019013274</ref><references group="xtra"/>
	[[Category: Aquifex aeolicus]]		[[Category: Aquifex aeolicus]]
	[[Category: Berger, J.]]		[[Category: Berger, J.]]
Line 23:		Line 24:
	[[Category: Helicase loader]]		[[Category: Helicase loader]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
			[[Category: Replication]]
	[[Category: Replication initiation factor]]		[[Category: Replication initiation factor]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 20:04:50 2009''~~