3eit - Revision history

OCA at 22:35, 26 March 2025

2025-03-26T22:35:04Z

← Older revision		Revision as of 22:35, 26 March 2025
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	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CIF_BURPS CIF_BURPS] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:~~19308257~~, PubMed:~~20688984~~, PubMed:~~19225106~~). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (PubMed:23175788). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (PubMed:20688984). Also regulates the host NF-kappa-B signaling via activation of MAPK/ERK cascade: activation of host MAPK/ERK cascade is independent of CRL complexes inhibition, suggesting that Cif has other host protein targets than NEDD8 (PubMed:28166272, PubMed:29848489).<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20688984</ref> <ref>PMID:21903097</ref> <ref>PMID:23175788</ref> <ref>PMID:28166272</ref> <ref>PMID:29848489</ref>		[https://www.uniprot.org/uniprot/CIF_BURPS CIF_BURPS] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19225106, PubMed:19308257, PubMed:20688984). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (PubMed:23175788). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (PubMed:20688984). Also regulates the host NF-kappa-B signaling via activation of MAPK/ERK cascade: activation of host MAPK/ERK cascade is independent of CRL complexes inhibition, suggesting that Cif has other host protein targets than NEDD8 (PubMed:28166272, PubMed:29848489).<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20688984</ref> <ref>PMID:21903097</ref> <ref>PMID:23175788</ref> <ref>PMID:28166272</ref> <ref>PMID:29848489</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:57, 22 November 2023

2023-11-22T11:57:44Z

← Older revision		Revision as of 11:57, 22 November 2023
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	<StructureSection load='3eit' size='340' side='right'caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='3eit' size='340' side='right'caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pseudomallei"_whitmore_1913 "bacillus~~ pseudomallei~~" whitmore 1913~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EIT FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~>, ~~<scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.56Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3eir\|3eir]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">YP_111397 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 "Bacillus pseudomallei" Whitmore 1913])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [https://pdbe.org/3eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [https://www.ebi.ac.uk/pdbsum/3eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [https://pdbe.org/3eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [https://www.ebi.ac.uk/pdbsum/3eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eit ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CIF_BURPS CIF_BURPS] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19308257, PubMed:20688984, PubMed:19225106). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (PubMed:23175788). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (PubMed:20688984). Also regulates the host NF-kappa-B signaling via activation of MAPK/ERK cascade: activation of host MAPK/ERK cascade is independent of CRL complexes inhibition, suggesting that Cif has other host protein targets than NEDD8 (PubMed:28166272, PubMed:29848489).<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20688984</ref> <ref>PMID:21903097</ref> <ref>PMID:23175788</ref> <ref>PMID:28166272</ref> <ref>PMID:29848489</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ pseudomallei ~~whitmore 1913~~]]		[[Category: Burkholderia pseudomallei]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Shao, F]]		[[Category: Shao F]]
	[[Category: Yao, Q]]		[[Category: Yao Q]]
	[[Category: Zhu, Y]]		[[Category: Zhu Y]]
	~~[[Category: Apain superfamily]]~~
	~~[[Category: Hydrolytic enzyme]]~~
	~~[[Category: Papain-like fold]]~~
	~~[[Category: Unknown function~~]]

OCA at 11:50, 16 February 2022

2022-02-16T11:50:26Z

← Older revision		Revision as of 11:50, 16 February 2022
Line 1:		Line 1:

	==the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei==		==the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei==
	<StructureSection load='3eit' size='340' side='right' caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='3eit' size='340' side='right'caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_pseudomallei"_whitmore_1913 "bacillus pseudomallei" whitmore 1913]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pseudomallei"_whitmore_1913 "bacillus pseudomallei" whitmore 1913]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EIT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3eir\|3eir]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 "Bacillus pseudomallei" Whitmore 1913])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 "Bacillus pseudomallei" Whitmore 1913])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [~~http~~://pdbe.org/3eit PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3eit PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3eit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [https://pdbe.org/3eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [https://www.ebi.ac.uk/pdbsum/3eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eit ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus pseudomallei whitmore 1913]]		[[Category: Bacillus pseudomallei whitmore 1913]]
			[[Category: Large Structures]]
	[[Category: Shao, F]]		[[Category: Shao, F]]
	[[Category: Yao, Q]]		[[Category: Yao, Q]]

OCA at 20:37, 4 August 2016

2016-08-04T20:37:17Z

← Older revision		Revision as of 20:37, 4 August 2016
Line 1:		Line 1:

	==the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei==		==the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei==
	<StructureSection load='3eit' size='340' side='right' caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>		<StructureSection load='3eit' size='340' side='right' caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_pseudomallei Burkholderia~~ pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pseudomallei"_whitmore_1913 "bacillus pseudomallei" whitmore 1913]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 ~~Burkholderia~~ pseudomallei])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 "Bacillus pseudomallei" Whitmore 1913])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [http://www.ebi.ac.uk/pdbsum/3eit PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [http://pdbe.org/3eit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [http://www.ebi.ac.uk/pdbsum/3eit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eit ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3eit" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Burkholderia~~ pseudomallei]]		[[Category: Bacillus pseudomallei whitmore 1913]]
	[[Category: Shao, F]]		[[Category: Shao, F]]
	[[Category: Yao, Q]]		[[Category: Yao, Q]]

OCA at 19:25, 24 December 2014

2014-12-24T19:25:22Z

← Older revision		Revision as of 19:25, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [http://www.ebi.ac.uk/pdbsum/3eit PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [http://www.ebi.ac.uk/pdbsum/3eit PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.,Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao R, Liu X, Huang N, Chen S, Liu L, Shao F Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3716-21. Epub 2009 Feb 18. PMID:19225106<ref>PMID:19225106</ref>		A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.,Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao R, Liu X, Huang N, Chen S, Liu L, Shao F Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3716-21. Epub 2009 Feb 18. PMID:19225106<ref>PMID:19225106</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Burkholderia pseudomallei]]		[[Category: Burkholderia pseudomallei]]
	[[Category: Shao, F.]]		[[Category: Shao, F]]
	[[Category: Yao, Q.]]		[[Category: Yao, Q]]
	[[Category: Zhu, Y.]]		[[Category: Zhu, Y]]
	[[Category: Apain superfamily]]		[[Category: Apain superfamily]]
	[[Category: Hydrolytic enzyme]]		[[Category: Hydrolytic enzyme]]
	[[Category: Papain-like fold]]		[[Category: Papain-like fold]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 09:39, 21 May 2014

2014-05-21T09:39:21Z

← Older revision		Revision as of 09:39, 21 May 2014
Line 1:		Line 1:
	[[~~Image~~:~~3eit~~.~~png~~\|~~left~~\|~~200px~~]]		==the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei==
			<StructureSection load='3eit' size='340' side='right' caption='[[3eit]], [[Resolution\|resolution]] 2.56Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eir\|3eir]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">YP_111397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eit RCSB], [http://www.ebi.ac.uk/pdbsum/3eit PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pathogenic bacteria deliver effector proteins into host cells through the type III secretion apparatus to modulate the host function. We identify a family of proteins, homologous to the type III effector Cif from enteropathogenic Escherichia coli, in pathogens including Yersinia, Photorhabdus, and Burkholderia that contain functional type III secretion systems. Like Cif, this family of proteins is capable of arresting the host cell cycle at G(2)/M. Structure of one of the family members, Cif homolog in Burkholderia pseudomallei (CHBP), reveals a papain-like fold and a conserved Cys-His-Gln catalytic triad despite the lack of primary sequence identity. For CHBP and Cif, only the putative catalytic Cys is susceptible to covalent modification by E-64, a specific inhibitor of papain-like cysteine proteases. Unlike papain-like enzymes where the S2 site is the major determinant of cleavage-site specificity, CHBP has a characteristic negatively charged pocket occupying surface areas corresponding to the S1/S1' site in papain-like proteases. The negative charge is provided by a conserved aspartate, and the pocket best fits an arginine, as revealed by molecular docking analysis. Mutation analysis establishes the essential role of the catalytic triad and the negatively charged pocket in inducing cell cycle arrest in host cells. Our results demonstrate that bacterial pathogens have evolved a unique papain-like hydrolytic activity to block the normal host cell cycle progression.

	<!--		A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.,Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao R, Liu X, Huang N, Chen S, Liu L, Shao F Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3716-21. Epub 2009 Feb 18. PMID:19225106<ref>PMID:19225106</ref>
	~~The line below this paragraph~~, ~~containing "STRUCTURE_3eit"~~, ~~creates the "Structure Box" on the page~~.
	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display~~.
	-->
	~~{{STRUCTURE_3eit\| PDB=3eit \| SCENE= }}~~

	~~===the 2.6 angstrom crystal structure~~ of ~~CHBP, the Cif Homologue from Burkholderia pseudomallei===~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
	~~<!--~~		<references/>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19225106}}, adds the Publication Abstract to~~ the ~~page~~		__TOC__
	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19225106 is the PubMed ID number~~.		</StructureSection>
	-->
	~~{{ABSTRACT_PUBMED_19225106}}~~

	~~==About this Structure==~~
	~~[[3eit]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA].~~

	==~~Reference~~==
	<~~ref group="xtra">PMID:019225106<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Burkholderia pseudomallei]]		[[Category: Burkholderia pseudomallei]]
	[[Category: Shao, F.]]		[[Category: Shao, F.]]

OCA: Protected "3eit" [edit=sysop:move=sysop]

2011-07-20T05:23:35Z

Protected "3eit" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:23, 20 July 2011
(No difference)

OCA at 05:23, 20 July 2011

2011-07-20T05:23:33Z

← Older revision		Revision as of 05:23, 20 July 2011
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	~~{{Seed}}~~
	[[Image:3eit.png\|left\|200px]]		[[Image:3eit.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
	~~3EIT~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA].		[[3eit]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19225106~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019225106</ref><references group="xtra"/>
	[[Category: Burkholderia pseudomallei]]		[[Category: Burkholderia pseudomallei]]
	[[Category: Shao, F.]]		[[Category: Shao, F.]]
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	[[Category: Papain-like fold]]		[[Category: Papain-like fold]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 8 20:07:10 2009''~~

OCA at 17:07, 8 April 2009

2009-04-08T17:07:10Z

← Older revision		Revision as of 17:07, 8 April 2009
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	{{Seed}}		{{Seed}}
	[[Image:3eit.~~jpg~~\|left\|200px]]		[[Image:3eit.png\|left\|200px]]

	<!--		<!--
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	===the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei===		===the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_19225106}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 19225106 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_19225106}}

	==About this Structure==		==About this Structure==
	3EIT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA].		3EIT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIT OCA].

			==Reference==
			<ref group="xtra">PMID:19225106</ref><references group="xtra"/>
	[[Category: Burkholderia pseudomallei]]		[[Category: Burkholderia pseudomallei]]
	[[Category: Shao, F.]]		[[Category: Shao, F.]]
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	[[Category: Unknown function]]		[[Category: Unknown function]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb~~ ~~4 11~~:34:48 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 8 20:07:10 2009''

OCA at 09:34, 4 February 2009

2009-02-04T09:34:53Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3eit is ON HOLD  until Paper Publication
+<!--
-Authors: Yao, Q., Zhu, Y.Q., Cui, J.X., Liu, X.Q., Shao, F.
+===the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 15 14:05:34 2008''
+==About this Structure==