3eks - Revision history

OCA at 09:48, 21 February 2024

2024-02-21T09:48:04Z

← Older revision		Revision as of 09:48, 21 February 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.		[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The fungal toxin cytochalasin D (CD) interferes with the normal dynamics of the actin cytoskeleton by binding to the barbed end of actin filaments. Despite its widespread use as a tool for studying actin-mediated processes, the exact location and nature of its binding to actin have not been previously determined. Here we describe two crystal structures of an expressed monomeric actin in complex with CD: one obtained by soaking preformed actin crystals with CD, and the other obtained by cocrystallization. The binding site for CD, in the hydrophobic cleft between actin subdomains 1 and 3, is the same in the two structures. Polar and hydrophobic contacts play equally important roles in CD binding, and six hydrogen bonds stabilize the actin-CD complex. Many unrelated actin-binding proteins and marine toxins target this cleft and the hydrophobic pocket at the front end of the cleft (viewing actin with subdomain 2 in the upper right corner). CD differs in that it binds to the back half of the cleft. The ability of CD to induce actin dimer formation and actin-catalyzed ATP hydrolysis may be related to its unique binding site and the necessity to fit its bulky macrocycle into this cleft. Contacts with residues lining this cleft appear to be crucial to capping and/or severing. The cocrystallized actin-CD structure also revealed changes in actin conformation. An approximately 6 degrees rotation of the smaller actin domain (subdomains 1 and 2) with respect to the larger domain (subdomains 3 and 4) results in small changes in crystal packing that allow the D-loop to adopt an extended loop structure instead of being disordered, as it is in most crystal structures of actin. We speculate that these changes represent a potential conformation that the actin monomer can adopt on the pathway to polymerization or in the filament.

	Crystal structures of monomeric actin bound to cytochalasin D.,Nair UB, Joel PB, Wan Q, Lowey S, Rould MA, Trybus KM J Mol Biol. 2008 Dec 26;384(4):848-64. Epub 2008 Oct 10. PMID:18938176<ref>PMID:18938176</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Actin 3D structures\|Actin 3D structures]]		*[[Actin 3D structures\|Actin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:13, 26 July 2023

2023-07-26T13:13:08Z

← Older revision		Revision as of 13:13, 26 July 2023
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	<StructureSection load='3eks' size='340' side='right'caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3eks' size='340' side='right'caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3eku\|3eku]], [[3el2\|3el2]]</div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [https://pdbe.org/3eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [https://www.ebi.ac.uk/pdbsum/3eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [https://pdbe.org/3eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [https://www.ebi.ac.uk/pdbsum/3eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.		[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Drome~~]]		[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Joel~~, P B~~]]		[[Category: Joel PB]]
	[[Category: Lowey, S]]		[[Category: Lowey S]]
	[[Category: Nair~~, U B~~]]		[[Category: Nair UB]]
	[[Category: Rould~~, M A~~]]		[[Category: Rould MA]]
	[[Category: Trybus~~, K M~~]]		[[Category: Trybus KM]]
	[[Category: Wan, Q]]		[[Category: Wan Q]]
	~~[[Category: Acetylation]]~~
	~~[[Category: Atp-binding]]~~
	~~[[Category: Atp-state]]~~
	~~[[Category: Contractile protein]]~~
	~~[[Category: Cytoplasm]]~~
	~~[[Category: Cytoskeleton]]~~
	~~[[Category: Fungal toxin]]~~
	~~[[Category: Motor protein]]~~
	~~[[Category: Nucleotide-binding~~]]

OCA at 19:42, 20 October 2021

2021-10-20T19:42:23Z

← Older revision		Revision as of 19:42, 20 October 2021
Line 1:		Line 1:

	==Crystal Structure of Monomeric Actin bound to Cytochalasin D==		==Crystal Structure of Monomeric Actin bound to Cytochalasin D==
	<StructureSection load='3eks' size='340' side='right' caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3eks' size='340' side='right'caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eku\|3eku]], [[3el2\|3el2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3eku\|3eku]], [[3el2\|3el2]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [~~http~~://pdbe.org/3eks PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3eks PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3eks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [https://pdbe.org/3eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [https://www.ebi.ac.uk/pdbsum/3eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.		[[https://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Actin 3D structures\|Actin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
			[[Category: Large Structures]]
	[[Category: Joel, P B]]		[[Category: Joel, P B]]
	[[Category: Lowey, S]]		[[Category: Lowey, S]]

OCA at 09:13, 25 October 2017

2017-10-25T09:13:45Z

← Older revision		Revision as of 09:13, 25 October 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Actin\|Actin]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 31:
	[[Category: Trybus, K M]]		[[Category: Trybus, K M]]
	[[Category: Wan, Q]]		[[Category: Wan, Q]]
			[[Category: Acetylation]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Atp-state]]		[[Category: Atp-state]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
			[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Fungal toxin]]		[[Category: Fungal toxin]]
	[[Category: Motor protein]]		[[Category: Motor protein]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]

OCA at 01:13, 5 August 2016

2016-08-05T01:13:25Z

← Older revision		Revision as of 01:13, 5 August 2016
Line 1:		Line 1:

	==Crystal Structure of Monomeric Actin bound to Cytochalasin D==		==Crystal Structure of Monomeric Actin bound to Cytochalasin D==
	<StructureSection load='3eks' size='340' side='right' caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3eks' size='340' side='right' caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eku\|3eku]], [[3el2\|3el2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eku\|3eku]], [[3el2\|3el2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [http://www.ebi.ac.uk/pdbsum/3eks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [http://pdbe.org/3eks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [http://www.ebi.ac.uk/pdbsum/3eks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3eks" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Drosophila melanogaster~~]]		[[Category: Drome]]
	[[Category: Joel, P B]]		[[Category: Joel, P B]]
	[[Category: Lowey, S]]		[[Category: Lowey, S]]

OCA at 21:30, 24 December 2014

2014-12-24T21:30:38Z

← Older revision		Revision as of 21:30, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [http://www.ebi.ac.uk/pdbsum/3eks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [http://www.ebi.ac.uk/pdbsum/3eks PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ACT1_DROME ACT1_DROME]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:13, 19 November 2014

2014-11-19T13:13:16Z

← Older revision		Revision as of 13:13, 19 November 2014
Line 1:		Line 1:
	[[~~Image~~:~~3eks~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of Monomeric Actin bound to Cytochalasin D==
			<StructureSection load='3eks' size='340' side='right' caption='[[3eks]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3eks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EKS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CY9:(3S,3AR,4S,6S,6AR,7E,10S,12R,13E,15R,15AR)-3-BENZYL-6,12-DIHYDROXY-4,10,12-TRIMETHYL-5-METHYLIDENE-1,11-DIOXO-2,3,3A,4,5,6,6A,9,10,11,12,15-DODECAHYDRO-1H-CYCLOUNDECA[D]ISOINDOL-15-YL+ACETATE'>CY9</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eku\|3eku]], [[3el2\|3el2]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Act5C, CG4027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eks RCSB], [http://www.ebi.ac.uk/pdbsum/3eks PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The fungal toxin cytochalasin D (CD) interferes with the normal dynamics of the actin cytoskeleton by binding to the barbed end of actin filaments. Despite its widespread use as a tool for studying actin-mediated processes, the exact location and nature of its binding to actin have not been previously determined. Here we describe two crystal structures of an expressed monomeric actin in complex with CD: one obtained by soaking preformed actin crystals with CD, and the other obtained by cocrystallization. The binding site for CD, in the hydrophobic cleft between actin subdomains 1 and 3, is the same in the two structures. Polar and hydrophobic contacts play equally important roles in CD binding, and six hydrogen bonds stabilize the actin-CD complex. Many unrelated actin-binding proteins and marine toxins target this cleft and the hydrophobic pocket at the front end of the cleft (viewing actin with subdomain 2 in the upper right corner). CD differs in that it binds to the back half of the cleft. The ability of CD to induce actin dimer formation and actin-catalyzed ATP hydrolysis may be related to its unique binding site and the necessity to fit its bulky macrocycle into this cleft. Contacts with residues lining this cleft appear to be crucial to capping and/or severing. The cocrystallized actin-CD structure also revealed changes in actin conformation. An approximately 6 degrees rotation of the smaller actin domain (subdomains 1 and 2) with respect to the larger domain (subdomains 3 and 4) results in small changes in crystal packing that allow the D-loop to adopt an extended loop structure instead of being disordered, as it is in most crystal structures of actin. We speculate that these changes represent a potential conformation that the actin monomer can adopt on the pathway to polymerization or in the filament.

	~~{{STRUCTURE_3eks\| PDB=3eks \| SCENE= }}~~		Crystal structures of monomeric actin bound to cytochalasin D.,Nair UB, Joel PB, Wan Q, Lowey S, Rould MA, Trybus KM J Mol Biol. 2008 Dec 26;384(4):848-64. Epub 2008 Oct 10. PMID:18938176<ref>PMID:18938176</ref>

	~~===Crystal Structure~~ of ~~Monomeric Actin bound to Cytochalasin D===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_18938176}}~~

	~~==About this Structure==~~
	~~[[3eks]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3EKS OCA].~~

	==See Also==		==See Also==
	*[[Actin\|Actin]]		*[[Actin\|Actin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:018938176<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Joel, P B.]]		[[Category: Joel, P B]]
	[[Category: Lowey, S.]]		[[Category: Lowey, S]]
	[[Category: Nair, U B.]]		[[Category: Nair, U B]]
	[[Category: Rould, M A.]]		[[Category: Rould, M A]]
	[[Category: Trybus, K M.]]		[[Category: Trybus, K M]]
	[[Category: Wan, Q.]]		[[Category: Wan, Q]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Atp-state]]		[[Category: Atp-state]]

OCA at 09:19, 20 October 2012

2012-10-20T09:19:36Z

← Older revision		Revision as of 09:19, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3eks]] is a 1 chain structure ~~of [[Actin]]~~ with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA].		[[3eks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA].

	==See Also==		==See Also==

OCA: Protected "3eks" [edit=sysop:move=sysop]

2012-07-25T22:28:47Z

Protected "3eks" [edit=sysop:move=sysop]

← Older revision	Revision as of 22:28, 25 July 2012
(No difference)

OCA at 22:28, 25 July 2012

2012-07-25T22:28:46Z

← Older revision		Revision as of 22:28, 25 July 2012
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	~~{{Seed}}~~
	[[Image:3eks.png\|left\|200px]]		[[Image:3eks.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3eks", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
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	{{STRUCTURE_3eks\| PDB=3eks \| SCENE= }}		{{STRUCTURE_3eks\| PDB=3eks \| SCENE= }}

	===Crystal Structure of Monomeric Actin bound to Cytochalasin D===		===Crystal Structure of Monomeric Actin bound to Cytochalasin D===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_18938176}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_18938176}}		{{ABSTRACT_PUBMED_18938176}}

	==About this Structure==		==About this Structure==
	~~3EKS~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA].		[[3eks]] is a 1 chain structure of [[Actin]] with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EKS OCA].

			==See Also==
			*[[Actin\|Actin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18938176~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018938176</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Joel, P B.]]		[[Category: Joel, P B.]]
Line 31:		Line 22:
	[[Category: Trybus, K M.]]		[[Category: Trybus, K M.]]
	[[Category: Wan, Q.]]		[[Category: Wan, Q.]]
	~~[[Category: Acetylation]]~~
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Atp-state]]		[[Category: Atp-state]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	~~[[Category: Cytoplasm]]~~
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Fungal toxin]]		[[Category: Fungal toxin]]
	[[Category: Motor protein]]		[[Category: Motor protein]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 09:04:52 2010''~~