3ets - Revision history

OCA at 06:34, 6 September 2023

2023-09-06T06:34:09Z

← Older revision		Revision as of 06:34, 6 September 2023
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	<StructureSection load='3ets' size='340' side='right'caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3ets' size='340' side='right'caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecol6 Ecol6~~]. The August 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_CFT073 Escherichia coli CFT073]. The August 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETS FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=4MU:7-HYDROXY~~-4~~-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene>~~</td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3elq\|3elq]]~~, ~~[[3ett\|3ett]]~~<~~/div></td></tr>~~
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=199310 ECOL6])</td></tr>~~
	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [https://pdbe.org/3ets PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [https://www.ebi.ac.uk/pdbsum/3ets PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ets ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [https://pdbe.org/3ets PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [https://www.ebi.ac.uk/pdbsum/3ets PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ets ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~B3HTA9_ECOLX B3HTA9_ECOLX]~~] Catalyzes the transfer of sulfuryl groups between phenolic compounds ~~(By similarity)~~.[HAMAP-Rule:MF_00933]		[https://www.uniprot.org/uniprot/ASST_ECOL6 ASST_ECOL6] Catalyzes the transfer of sulfuryl groups between phenolic compounds.[HAMAP-Rule:MF_00933]<ref>PMID:18565543</ref> <ref>PMID:19036922</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase 3D structures\|Sulfotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Aryl-sulfate sulfotransferase]]~~		[[Category: Escherichia coli CFT073]]
	~~[[Category: Ecol6~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Sulfotransferases]]		[[Category: Sulfotransferases]]
	[[Category: Glockshuber, R]]		[[Category: Glockshuber R]]
	[[Category: Grimshaw~~, J P~~]]		[[Category: Grimshaw JP]]
	[[Category: Malojcic, G]]		[[Category: Malojcic G]]
	[[Category: Owen~~, R L]]~~		[[Category: Owen RL]]
	~~[[Category: 4-methylumbelliferylsulfate]]~~
	~~[[Category: Bacteria]]~~
	~~[[Category: Beta propeller]]~~
	~~[[Category: Periplasm]]~~
	~~[[Category: Phenol]]~~
	~~[[Category: Protein-substrate complex]]~~
	~~[[Category: Sulfate]]~~
	~~[[Category: Sulfohistidine]]~~
	~~[[Category: Transesterification]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Transferase 4-methylumbelliferone~~]]

OCA at 09:16, 23 February 2022

2022-02-23T09:16:03Z

← Older revision		Revision as of 09:16, 23 February 2022
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	==Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site==		==Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site==
	<StructureSection load='3ets' size='340' side='right' caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3ets' size='340' side='right'caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecol6 Ecol6]. The August 2009 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ETS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecol6 Ecol6]. The August 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3elq\|3elq]], [[3ett\|3ett]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3elq\|3elq]], [[3ett\|3ett]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=199310 ECOL6])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=199310 ECOL6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [~~http~~://pdbe.org/3ets PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ets PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ets ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [https://pdbe.org/3ets PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [https://www.ebi.ac.uk/pdbsum/3ets PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ets ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/B3HTA9_ECOLX B3HTA9_ECOLX]] Catalyzes the transfer of sulfuryl groups between phenolic compounds (By similarity).[HAMAP-Rule:MF_00933]		[[https://www.uniprot.org/uniprot/B3HTA9_ECOLX B3HTA9_ECOLX]] Catalyzes the transfer of sulfuryl groups between phenolic compounds (By similarity).[HAMAP-Rule:MF_00933]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 3ets" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ets" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Sulfotransferase\|Sulfotransferase]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Aryl-sulfate sulfotransferase]]		[[Category: Aryl-sulfate sulfotransferase]]
	[[Category: Ecol6]]		[[Category: Ecol6]]
			[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Sulfotransferases]]		[[Category: Sulfotransferases]]

OCA at 09:19, 25 October 2017

2017-10-25T09:19:31Z

← Older revision		Revision as of 09:19, 25 October 2017
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	</div>		</div>
	<div class="pdbe-citations 3ets" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ets" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Sulfotransferase\|Sulfotransferase]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bacteria]]		[[Category: Bacteria]]
	[[Category: Beta propeller]]		[[Category: Beta propeller]]
			[[Category: Periplasm]]
	[[Category: Phenol]]		[[Category: Phenol]]
	[[Category: Protein-substrate complex]]		[[Category: Protein-substrate complex]]

OCA at 12:10, 4 August 2016

2016-08-04T12:10:58Z

← Older revision		Revision as of 12:10, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site==		==Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site==
	<StructureSection load='3ets' size='340' side='right' caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3ets' size='340' side='right' caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ETS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecol6 Ecol6]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ETS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3elq\|3elq]], [[3ett\|3ett]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3elq\|3elq]], [[3ett\|3ett]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=199310 ECOL6])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [http://www.ebi.ac.uk/pdbsum/3ets PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [http://pdbe.org/3ets PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [http://www.ebi.ac.uk/pdbsum/3ets PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ets ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ets" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Aryl-sulfate sulfotransferase]]		[[Category: Aryl-sulfate sulfotransferase]]
	[[Category: ~~Escherichia coli~~]]		[[Category: Ecol6]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Sulfotransferases]]		[[Category: Sulfotransferases]]

OCA at 09:11, 25 December 2014

2014-12-25T09:11:34Z

← Older revision		Revision as of 09:11, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [http://www.ebi.ac.uk/pdbsum/3ets PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [http://www.ebi.ac.uk/pdbsum/3ets PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/B3HTA9_ECOLX B3HTA9_ECOLX]] Catalyzes the transfer of sulfuryl groups between phenolic compounds (By similarity).[HAMAP-Rule:MF_00933]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:40, 26 November 2014

2014-11-26T08:40:19Z

← Older revision		Revision as of 08:40, 26 November 2014
Line 1:		Line 1:
	[[~~Image~~:~~3ets~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site==
			<StructureSection load='3ets' size='340' side='right' caption='[[3ets]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ETS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4MU:7-HYDROXY-4-METHYL-2H-CHROMEN-2-ONE'>4MU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HS8:3-(1-SULFO-1H-IMIDAZOL-3-IUM-4-YL)-L-ALANINE'>HS8</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3elq\|3elq]], [[3ett\|3ett]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASTA, EcF11_2091 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl-sulfate_sulfotransferase Aryl-sulfate sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.22 2.8.2.22] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ets OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ets RCSB], [http://www.ebi.ac.uk/pdbsum/3ets PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Sulfotransferases are a versatile class of enzymes involved in numerous physiological processes. In mammals, adenosine 3'-phosphate-5'-phosphosulfate (PAPS) is the universal sulfuryl donor, and PAPS-dependent sulfurylation of small molecules, including hormones, sugars, and antibiotics, is a critical step in hepatic detoxification and extracellular signaling. In contrast, little is known about sulfotransferases in bacteria, which make use of sulfurylated molecules as mediators of cell-cell interactions and host-pathogen interactions. Bacterial arylsulfate sulfotransferases (also termed aryl sulfotransferases), in contrast to PAPS-dependent sulfotransferases, transfer sulfuryl groups exclusively among phenolic compounds in a PAPS-independent manner. Here, we report the crystal structure of the virulence factor arylsulfate sulfotransferase (ASST) from the prototypic, pyelonephritogenic Escherichia coli strain CFT073 at 2.0-A resolution, and 2 catalytic intermediates, at 2.1-A and 2.4-A resolution, with substrates bound in the active site. ASST is one of the largest periplasmic enzymes and its 3D structure differs fundamentally from all other structurally characterized sulfotransferases. Each 63.8-kDa subunit of the ASST homodimer comprises a 6-bladed beta-propeller domain and a C-terminal beta-sandwich domain. The active sites of the dimer are situated at the center of the channel formed by each beta-propeller and are defined by the side chains of His-252, His-356, Arg-374, and His-436. We show that ASST follows a ping-pong bi-bi reaction mechanism, in which the catalytic residue His-436 undergoes transient sulfurylation, a previously unreported covalent protein modification. The data provide a framework for understanding PAPS-independent sulfotransfer and a basis for drug design targeting this bacterial virulence factor.

	~~{{STRUCTURE_3ets\| PDB=3ets \| SCENE= }}~~		A structural and biochemical basis for PAPS-independent sulfuryl transfer by aryl sulfotransferase from uropathogenic Escherichia coli.,Malojcic G, Owen RL, Grimshaw JP, Brozzo MS, Dreher-Teo H, Glockshuber R Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19217-22. Epub 2008 Nov 26. PMID:19036922<ref>PMID:19036922</ref>

	~~===Crystal structure~~ of ~~a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in~~ the ~~active site===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19036922}}~~

	~~==About this Structure==~~
	~~[[3ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static~~.~~do?p=education_discussion/molecule_of_the_month/index~~.~~html Molecule~~ of ~~the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx~~.~~doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA].~~

	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase\|Sulfotransferase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019036922<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Aryl-sulfate sulfotransferase]]		[[Category: Aryl-sulfate sulfotransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Sulfotransferases]]		[[Category: Sulfotransferases]]
	[[Category: Glockshuber, R.]]		[[Category: Glockshuber, R]]
	[[Category: Grimshaw, J P.]]		[[Category: Grimshaw, J P]]
	[[Category: Malojcic, G.]]		[[Category: Malojcic, G]]
	[[Category: Owen, R L.]]		[[Category: Owen, R L]]
	[[Category: 4-methylumbelliferylsulfate]]		[[Category: 4-methylumbelliferylsulfate]]
	[[Category: Bacteria]]		[[Category: Bacteria]]

OCA at 14:58, 20 October 2012

2012-10-20T14:58:01Z

← Older revision		Revision as of 14:58, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3ets]] is a 2 chain structure ~~of [[Sulfotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA].		[[3ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA].

	==See Also==		==See Also==

OCA: Protected "3ets" [edit=sysop:move=sysop]

2012-07-26T16:51:18Z

Protected "3ets" [edit=sysop:move=sysop]

← Older revision	Revision as of 16:51, 26 July 2012
(No difference)

OCA at 16:51, 26 July 2012

2012-07-26T16:51:16Z

← Older revision		Revision as of 16:51, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3ets.png\|left\|200px]]		[[Image:3ets.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3ets", creates the "Structure Box" on the page.~~
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	{{STRUCTURE_3ets\| PDB=3ets \| SCENE= }}		{{STRUCTURE_3ets\| PDB=3ets \| SCENE= }}

	===Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site===		===Crystal structure of a bacterial arylsulfate sulfotransferase catalytic intermediate with 4-methylumbelliferone bound in the active site===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19036922}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_19036922}}		{{ABSTRACT_PUBMED_19036922}}

	==About this Structure==		==About this Structure==
	~~3ETS~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA].		[[3ets]] is a 2 chain structure of [[Sulfotransferase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sulfotransferases'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_8 10.2210/rcsb_pdb/mom_2009_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETS OCA].

			==See Also==
			*[[Sulfotransferase\|Sulfotransferase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19036922~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019036922</ref><references group="xtra"/>
	[[Category: Aryl-sulfate sulfotransferase]]		[[Category: Aryl-sulfate sulfotransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 35:		Line 26:
	[[Category: Bacteria]]		[[Category: Bacteria]]
	[[Category: Beta propeller]]		[[Category: Beta propeller]]
	~~[[Category: Periplasm]]~~
	[[Category: Phenol]]		[[Category: Phenol]]
	[[Category: Protein-substrate complex]]		[[Category: Protein-substrate complex]]
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	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Transferase 4-methylumbelliferone]]		[[Category: Transferase 4-methylumbelliferone]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 08:36:51 2010''~~

OCA at 06:36, 21 January 2010

2010-01-21T06:36:54Z

← Older revision		Revision as of 06:36, 21 January 2010
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	[[Category: Aryl-sulfate sulfotransferase]]		[[Category: Aryl-sulfate sulfotransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
			[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Sulfotransferases]]		[[Category: Sulfotransferases]]
	[[Category: Glockshuber, R.]]		[[Category: Glockshuber, R.]]
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	[[Category: Transferase 4-methylumbelliferone]]		[[Category: Transferase 4-methylumbelliferone]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jan ~~20 10~~:47:44 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 08:36:51 2010''