3fpp - Revision history

OCA at 08:25, 20 March 2024

2024-03-20T08:25:20Z

← Older revision		Revision as of 08:25, 20 March 2024
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	<StructureSection load='3fpp' size='340' side='right'caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='3fpp' size='340' side='right'caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPP FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>macA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895~~])</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.99Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [https://pdbe.org/3fpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [https://www.ebi.ac.uk/pdbsum/3fpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [https://pdbe.org/3fpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [https://www.ebi.ac.uk/pdbsum/3fpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref>		[https://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpp ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	In Gram-negative bacteria, type I protein secretion systems and tripartite drug efflux pumps have a periplasmic membrane fusion protein (MFP) as an essential component. MFPs bridge the outer membrane factor and an inner membrane transporter, although the oligomeric state of MFPs remains unclear. The most characterized MFP AcrA connects the outer membrane factor TolC and the resistance-nodulation-division-type efflux transporter AcrB, which is a major multidrug efflux pump in Escherichia coli. MacA is the periplasmic MFP in the MacAB-TolC pump, where MacB was characterized as a macrolide-specific ATP-binding-cassette-type efflux transporter. Here, we report the crystal structure of E. coli MacA and the experimentally phased map of Actinobacillus actinomycetemcomitans MacA, which reveal a domain orientation of MacA different from that of AcrA. Notably, a hexameric assembly of MacA was found in both crystals, exhibiting a funnel-like structure with a central channel and a conical mouth. The hexameric MacA assembly was further confirmed by electron microscopy and functional studies in vitro and in vivo. The hexameric structure of MacA provides insight into the oligomeric state in the functional complex of the drug efflux pump and type I secretion system.

	Crystal structure of the periplasmic component of a tripartite macrolide-specific efflux pump.,Yum S, Xu Y, Piao S, Sim SH, Kim HM, Jo WS, Kim KJ, Kweon HS, Jeong MH, Jeon H, Lee K, Ha NC J Mol Biol. 2009 Apr 17;387(5):1286-97. Epub 2009 Feb 28. PMID:19254725<ref>PMID:19254725</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3fpp" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ha, N C]]		[[Category: Ha N-C]]
	[[Category: Piao, S]]		[[Category: Piao S]]
	[[Category: Xu, Y]]		[[Category: Xu Y]]
	[[Category: Yum, S]]		[[Category: Yum S]]
	~~[[Category: Antibiotic resistance]]~~
	~~[[Category: Cell inner membrane]]~~
	~~[[Category: Cell membrane]]~~
	~~[[Category: Drug efflux pump]]~~
	~~[[Category: Hexameric assembly]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Membrane fusion protein]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Periplasmic protein]]~~
	~~[[Category: Transport~~]]

OCA at 08:07, 2 March 2022

2022-03-02T08:07:38Z

← Older revision		Revision as of 08:07, 2 March 2022
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	==Crystal structure of E.coli MacA==		==Crystal structure of E.coli MacA==
	<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='3fpp' size='340' side='right'caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3FPP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPP FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [~~http~~://pdbe.org/3fpp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3fpp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [https://pdbe.org/3fpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [https://www.ebi.ac.uk/pdbsum/3fpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref>		[[https://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
			[[Category: Large Structures]]
	[[Category: Ha, N C]]		[[Category: Ha, N C]]
	[[Category: Piao, S]]		[[Category: Piao, S]]

OCA at 07:41, 5 December 2018

2018-12-05T07:41:28Z

← Older revision		Revision as of 07:41, 5 December 2018
Line 1:		Line 1:

	==Crystal structure of E.coli MacA==		==Crystal structure of E.coli MacA==
	<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPP FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://pdbe.org/3fpp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://pdbe.org/3fpp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpp_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 00:51, 8 February 2016

2016-02-08T00:51:24Z

← Older revision		Revision as of 00:51, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>		<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPP FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://pdbe.org/3fpp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3fpp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia~~ coli]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Ha, N C]]		[[Category: Ha, N C]]
	[[Category: Piao, S]]		[[Category: Piao, S]]

OCA at 19:21, 24 December 2014

2014-12-24T19:21:39Z

← Older revision		Revision as of 19:21, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:28, 3 December 2014

2014-12-03T10:28:35Z

← Older revision		Revision as of 10:28, 3 December 2014
Line 1:		Line 1:
	[[Image:~~3fpp~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of E.coli MacA==
			<StructureSection load='3fpp' size='340' side='right' caption='[[3fpp]], [[Resolution\|resolution]] 2.99Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPP FirstGlance]. <br>
			</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fpp RCSB], [http://www.ebi.ac.uk/pdbsum/3fpp PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpp_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			In Gram-negative bacteria, type I protein secretion systems and tripartite drug efflux pumps have a periplasmic membrane fusion protein (MFP) as an essential component. MFPs bridge the outer membrane factor and an inner membrane transporter, although the oligomeric state of MFPs remains unclear. The most characterized MFP AcrA connects the outer membrane factor TolC and the resistance-nodulation-division-type efflux transporter AcrB, which is a major multidrug efflux pump in Escherichia coli. MacA is the periplasmic MFP in the MacAB-TolC pump, where MacB was characterized as a macrolide-specific ATP-binding-cassette-type efflux transporter. Here, we report the crystal structure of E. coli MacA and the experimentally phased map of Actinobacillus actinomycetemcomitans MacA, which reveal a domain orientation of MacA different from that of AcrA. Notably, a hexameric assembly of MacA was found in both crystals, exhibiting a funnel-like structure with a central channel and a conical mouth. The hexameric MacA assembly was further confirmed by electron microscopy and functional studies in vitro and in vivo. The hexameric structure of MacA provides insight into the oligomeric state in the functional complex of the drug efflux pump and type I secretion system.

	~~{{STRUCTURE_3fpp\| PDB=3fpp \| SCENE= }}~~		Crystal structure of the periplasmic component of a tripartite macrolide-specific efflux pump.,Yum S, Xu Y, Piao S, Sim SH, Kim HM, Jo WS, Kim KJ, Kweon HS, Jeong MH, Jeon H, Lee K, Ha NC J Mol Biol. 2009 Apr 17;387(5):1286-97. Epub 2009 Feb 28. PMID:19254725<ref>PMID:19254725</ref>

	~~===Crystal structure~~ of E.~~coli MacA===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19254725}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3fpp]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019254725<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ha, N C.]]		[[Category: Ha, N C]]
	[[Category: Piao, S.]]		[[Category: Piao, S]]
	[[Category: Xu, Y.]]		[[Category: Xu, Y]]
	[[Category: Yum, S.]]		[[Category: Yum, S]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Cell inner membrane]]		[[Category: Cell inner membrane]]

OCA: Protected "3fpp" [edit=sysop:move=sysop]

2013-02-20T14:50:25Z

Protected "3fpp" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:50, 20 February 2013
(No difference)

OCA at 14:50, 20 February 2013

2013-02-20T14:50:23Z

← Older revision		Revision as of 14:50, 20 February 2013
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	~~{{Seed}}~~
	[[Image:3fpp.png\|left\|200px]]		[[Image:3fpp.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3fpp", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3fpp\| PDB=3fpp \| SCENE= }}		{{STRUCTURE_3fpp\| PDB=3fpp \| SCENE= }}

	===Crystal structure of E.coli MacA===		===Crystal structure of E.coli MacA===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19254725}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19254725 is the PubMed ID number.~~
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	{{ABSTRACT_PUBMED_19254725}}		{{ABSTRACT_PUBMED_19254725}}

	==About this Structure==		==About this Structure==
	~~3FPP~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA].		[[3fpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPP OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19254725~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019254725</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ha, N C.]]		[[Category: Ha, N C.]]
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	[[Category: Periplasmic protein]]		[[Category: Periplasmic protein]]
	[[Category: Transport]]		[[Category: Transport]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:23:53 2009''~~

OCA at 07:23, 6 May 2009

2009-05-06T07:23:54Z

← Older revision		Revision as of 07:23, 6 May 2009
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	{{Seed}}		{{Seed}}
	[[Image:3fpp.~~jpg~~\|left\|200px]]		[[Image:3fpp.png\|left\|200px]]

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	[[Category: Transport]]		[[Category: Transport]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Apr 30~~ 10:05:28 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:23:53 2009''

OCA at 07:05, 30 April 2009

2009-04-30T07:05:28Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3fpp is ON HOLD  until Paper Publication
+<!--
-Authors: Yum, S., Xu, Y., Piao, S., Sim, S.-H., Kim, K.-J., Kweon, H.-S., Jeon, H., Lee, K., Ha, N.-C.
+===Crystal structure of E.coli MacA===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 11 12:35:26 2009''
+<!--