3gee - Revision history

OCA at 15:39, 1 November 2023

2023-11-01T15:39:01Z

← Older revision		Revision as of 15:39, 1 November 2023
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	<StructureSection load='3gee' size='340' side='right'caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='3gee' size='340' side='right'caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_49652 Atcc 49652~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GEE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.95Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 ATCC 49652])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [https://pdbe.org/3gee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [https://www.ebi.ac.uk/pdbsum/3gee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gee ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [https://pdbe.org/3gee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [https://www.ebi.ac.uk/pdbsum/3gee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/MNME_CHLTE MNME_CHLTE]] Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.[HAMAP-Rule:MF_00379]		[https://www.uniprot.org/uniprot/MNME_CHLTE MNME_CHLTE] Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.[HAMAP-Rule:MF_00379]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 49652~~]]		[[Category: Chlorobaculum tepidum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Meyer, S]]		[[Category: Meyer S]]
	[[Category: Wittinghofer, A]]		[[Category: Wittinghofer A]]
	~~[[Category: Cytoplasm]]~~
	~~[[Category: G protein]]~~
	~~[[Category: Gtp-binding]]~~
	~~[[Category: Gtpase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Magnesium]]~~
	~~[[Category: Metal-binding]]~~
	~~[[Category: Nucleotide-binding]]~~
	~~[[Category: Potassium]]~~
	~~[[Category: Trna modification]]~~
	~~[[Category: Trna processing]]~~
	~~[[Category: U34~~]]

OCA at 08:02, 9 March 2022

2022-03-09T08:02:47Z

← Older revision		Revision as of 08:02, 9 March 2022
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	==Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID==		==Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID==
	<StructureSection load='3gee' size='340' side='right' caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='3gee' size='340' side='right'caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_49652 Atcc 49652]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3GEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_49652 Atcc 49652]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GEE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 ATCC 49652])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 ATCC 49652])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [~~http~~://pdbe.org/3gee PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3gee PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3gee ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [https://pdbe.org/3gee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [https://www.ebi.ac.uk/pdbsum/3gee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/MNME_CHLTE MNME_CHLTE]] Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.[HAMAP-Rule:MF_00379]		[[https://www.uniprot.org/uniprot/MNME_CHLTE MNME_CHLTE]] Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.[HAMAP-Rule:MF_00379]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 49652]]		[[Category: Atcc 49652]]
			[[Category: Large Structures]]
	[[Category: Meyer, S]]		[[Category: Meyer, S]]
	[[Category: Wittinghofer, A]]		[[Category: Wittinghofer, A]]

OCA at 05:14, 7 December 2018

2018-12-07T05:14:52Z

← Older revision		Revision as of 05:14, 7 December 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/3gee_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/3gee_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Meyer, S]]		[[Category: Meyer, S]]
	[[Category: Wittinghofer, A]]		[[Category: Wittinghofer, A]]
			[[Category: Cytoplasm]]
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]

OCA at 05:23, 5 August 2016

2016-08-05T05:23:08Z

← Older revision		Revision as of 05:23, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID==		==Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID==
	<StructureSection load='3gee' size='340' side='right' caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='3gee' size='340' side='right' caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlorobaculum_tepidum Chlorobaculum tepidum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49652 Atcc 49652]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GEE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 ~~Chlorobaculum tepidum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 ATCC 49652])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [http://www.ebi.ac.uk/pdbsum/3gee PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [http://pdbe.org/3gee PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [http://www.ebi.ac.uk/pdbsum/3gee PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gee ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3gee" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chlorobaculum tepidum~~]]		[[Category: Atcc 49652]]
	[[Category: Meyer, S]]		[[Category: Meyer, S]]
	[[Category: Wittinghofer, A]]		[[Category: Wittinghofer, A]]

OCA at 16:06, 25 December 2014

2014-12-25T16:06:16Z

← Older revision		Revision as of 16:06, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [http://www.ebi.ac.uk/pdbsum/3gee PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [http://www.ebi.ac.uk/pdbsum/3gee PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MNME_CHLTE MNME_CHLTE]] Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.[HAMAP-Rule:MF_00379]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:48, 8 December 2014

2014-12-08T08:48:02Z

← Older revision		Revision as of 08:48, 8 December 2014
Line 1:		Line 1:
	[[Image:~~3gee~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID==
			<StructureSection load='3gee' size='340' side='right' caption='[[3gee]], [[Resolution\|resolution]] 2.95Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3gee]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GEE FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rfl\|1rfl]], [[1xzp\|1xzp]], [[1xzq\|1xzq]], [[2gja\|2gja]], [[2gj8\|2gj8]], [[2gj9\|2gj9]], [[3cp2\|3cp2]], [[3cp8\|3cp8]], [[3geh\|3geh]], [[3gei\|3gei]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mnmE, CT2084, thdF, trmE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1097 Chlorobaculum tepidum])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gee RCSB], [http://www.ebi.ac.uk/pdbsum/3gee PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/3gee_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			MnmE, which is involved in the modification of the wobble position of certain tRNAs, belongs to the expanding class of G proteins activated by nucleotide-dependent dimerization (GADs). Previous models suggested the protein to be a multidomain protein whose G domains contact each other in a nucleotide dependent manner. Here we employ a combined approach of X-ray crystallography and pulse electron paramagnetic resonance (EPR) spectroscopy to show that large domain movements are coupled to the G protein cycle of MnmE. The X-ray structures show MnmE to be a constitutive homodimer where the highly mobile G domains face each other in various orientations but are not in close contact as suggested by the GDP-AlF(x) structure of the isolated domains. Distance measurements by pulse double electron-electron resonance (DEER) spectroscopy show that the G domains adopt an open conformation in the nucleotide free/GDP-bound and an open/closed two-state equilibrium in the GTP-bound state, with maximal distance variations of 18 A. With GDP and AlF(x), which mimic the transition state of the phosphoryl transfer reaction, only the closed conformation is observed. Dimerization of the active sites with GDP-AlF(x) requires the presence of specific monovalent cations, thus reflecting the requirements for the GTPase reaction of MnmE. Our results directly demonstrate the nature of the conformational changes MnmE was previously suggested to undergo during its GTPase cycle. They show the nucleotide-dependent dynamic movements of the G domains around two swivel positions relative to the rest of the protein, and they are of crucial importance for understanding the mechanistic principles of this GAD.

	~~{{STRUCTURE_3gee\| PDB=3gee \| SCENE= }}~~		Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy.,Meyer S, Bohme S, Kruger A, Steinhoff HJ, Klare JP, Wittinghofer A PLoS Biol. 2009 Oct;7(10):e1000212. Epub 2009 Oct 6. PMID:19806182<ref>PMID:19806182</ref>

	~~===Crystal structure~~ of ~~MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19806182}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3gee]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019806182<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Chlorobaculum tepidum]]		[[Category: Chlorobaculum tepidum]]
	[[Category: Meyer, S.]]		[[Category: Meyer, S]]
	[[Category: Wittinghofer, A.]]		[[Category: Wittinghofer, A]]
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]

OCA: Protected "3gee" [edit=sysop:move=sysop]

2013-02-20T14:24:46Z

Protected "3gee" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:24, 20 February 2013
(No difference)

OCA at 14:24, 20 February 2013

2013-02-20T14:24:44Z

← Older revision		Revision as of 14:24, 20 February 2013
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	~~{{Seed}}~~
	[[Image:3gee.png\|left\|200px]]		[[Image:3gee.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3gee", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
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	{{STRUCTURE_3gee\| PDB=3gee \| SCENE= }}		{{STRUCTURE_3gee\| PDB=3gee \| SCENE= }}

	===Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID===		===Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19806182}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19806182 is the PubMed ID number.~~
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	{{ABSTRACT_PUBMED_19806182}}		{{ABSTRACT_PUBMED_19806182}}

	==About this Structure==		==About this Structure==
	~~3GEE~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA].		[[3gee]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19806182~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019806182</ref><references group="xtra"/>
	[[Category: Chlorobaculum tepidum]]		[[Category: Chlorobaculum tepidum]]
	[[Category: Meyer, S.]]		[[Category: Meyer, S.]]
	[[Category: Wittinghofer, A.]]		[[Category: Wittinghofer, A.]]
	~~[[Category: Cytoplasm]]~~
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]
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	[[Category: Trna processing]]		[[Category: Trna processing]]
	[[Category: U34]]		[[Category: U34]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:34:58 2010''~~

OCA at 12:34, 28 January 2010

2010-01-28T12:34:59Z

← Older revision		Revision as of 12:34, 28 January 2010
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	{{Seed}}		{{Seed}}
	[[Image:3gee.~~jpg~~\|left\|200px]]		[[Image:3gee.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
	3GEE is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA].		3GEE is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorobaculum_tepidum Chlorobaculum tepidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GEE OCA].

	==Reference==		==Reference==
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	[[Category: U34]]		[[Category: U34]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Oct~~ 28 13:25:~~15 2009~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:34:58 2010''

OCA at 11:25, 28 October 2009

2009-10-28T11:25:20Z

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-'''Unreleased structure'''
+{{Seed}}
-The entry 3gee is ON HOLD  until Paper Publication
+<!--
-Authors: Meyer, S., Boehme, S., Krueger, A., Dvorsky, R., Steinhoff, H.J., Klare, J.P., Wittinghofer, A.
+===Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep  3 15:18:16 2009''
+<!--