3hl4 - Revision history

OCA at 10:00, 21 February 2024

2024-02-21T10:00:30Z

← Older revision		Revision as of 10:00, 21 February 2024
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	<StructureSection load='3hl4' size='340' side='right'caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3hl4' size='340' side='right'caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CDC:~~[2~~-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Ctpct, Pcyt1, Pcyt1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=10116 Buffalo rat~~]~~)</td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [https://pdbe.org/3hl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [https://www.ebi.ac.uk/pdbsum/3hl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [https://pdbe.org/3hl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [https://www.ebi.ac.uk/pdbsum/3hl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PCY1A_RAT PCY1A_RAT]] Controls phosphatidylcholine synthesis.		[https://www.uniprot.org/uniprot/PCY1A_RAT PCY1A_RAT] Controls phosphatidylcholine synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hl4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hl4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	CTP:phosphocholine cytidylyltransferase (CCT) is the key regulatory enzyme in the synthesis of phosphatidylcholine, the most abundant phospholipid in eukaryotic cell membranes. The CCT-catalyzed transfer of a cytidylyl group from CTP to phosphocholine to form CDP-choline is regulated by a membrane lipid-dependent mechanism imparted by its C-terminal membrane binding domain. We present the first analysis of a crystal structure of a eukaryotic CCT. A deletion construct of rat CCTalpha spanning residues 1-236 (CCT236) lacks the regulatory domain and as a result displays constitutive activity. The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline. Each chain is composed of a complete catalytic domain with an intimately associated N-terminal extension, which together with the catalytic domain contributes to the dimer interface. Although the CCT236 structure reveals elements involved in binding cytidine that are conserved with other members of the cytidylyltransferase superfamily, it also features nonconserved active site residues, His-168 and Tyr-173, that make key interactions with the beta-phosphate of CDP-choline. Mutagenesis and kinetic analyses confirmed their role in phosphocholine binding and catalysis. These results demonstrate structural and mechanistic differences in a broadly conserved protein fold across the cytidylyltransferase family. Comparison of the CCT236 structure with those of other nucleotidyltransferases provides evidence for substrate-induced active site loop movements and a disorder-to-order transition of a loop element in the catalytic mechanism.

	Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.,Lee J, Johnson J, Ding Z, Paetzel M, Cornell RB J Biol Chem. 2009 Nov 27;284(48):33535-48. Epub 2009 Sep 25. PMID:19783652<ref>PMID:19783652</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3hl4" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Buffalo rat]]~~
	~~[[Category: Choline-phosphate cytidylyltransferase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Cornell, R B~~]]		[[Category: Rattus norvegicus]]
	[[Category: ~~Lee, J~~]]		[[Category: Cornell RB]]
	[[Category: ~~Paetzel, M~~]]		[[Category: Lee J]]
	[[Category: ~~Amphitropic protein]]~~		[[Category: Paetzel M]]
	~~[[Category: Cdp-choline]]~~
	~~[[Category: Ctp]]~~
	~~[[Category: Cytidylyltransferase]]~~
	~~[[Category: Lipid metabolism]]~~
	~~[[Category: Nucleotidyltransferase]]~~
	~~[[Category: Phosphatidylcholine]]~~
	~~[[Category: Phosphocholine]]~~
	~~[[Category: Phospholipid synthesis]]~~
	~~[[Category: Phosphoprotein]]~~
	~~[[Category: Rossmann fold]]~~
	~~[[Category: Transferase~~]]

OCA at 12:51, 23 March 2022

2022-03-23T12:51:22Z

← Older revision		Revision as of 12:51, 23 March 2022
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	==Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline==		==Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline==
	<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3hl4' size='340' side='right'caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [~~http~~://pdbe.org/3hl4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hl4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [https://pdbe.org/3hl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [https://www.ebi.ac.uk/pdbsum/3hl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PCY1A_RAT PCY1A_RAT]] Controls phosphatidylcholine synthesis.		[[https://www.uniprot.org/uniprot/PCY1A_RAT PCY1A_RAT]] Controls phosphatidylcholine synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Choline-phosphate cytidylyltransferase]]		[[Category: Choline-phosphate cytidylyltransferase]]
			[[Category: Large Structures]]
	[[Category: Cornell, R B]]		[[Category: Cornell, R B]]
	[[Category: Lee, J]]		[[Category: Lee, J]]

OCA at 07:55, 12 December 2018

2018-12-12T07:55:39Z

← Older revision		Revision as of 07:55, 12 December 2018
Line 1:		Line 1:

	==Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline==		==Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline==
	<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://pdbe.org/3hl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://pdbe.org/3hl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hl4_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hl4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:10, 9 February 2016

2016-02-09T13:10:53Z

← Older revision		Revision as of 13:10, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 ~~Rattus norvegicus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://pdbe.org/3hl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hl4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3hl4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Buffalo rat]]
	[[Category: Choline-phosphate cytidylyltransferase]]		[[Category: Choline-phosphate cytidylyltransferase]]
	~~[[Category: Rattus norvegicus]]~~
	[[Category: Cornell, R B]]		[[Category: Cornell, R B]]
	[[Category: Lee, J]]		[[Category: Lee, J]]

OCA at 07:01, 25 December 2014

2014-12-25T07:01:35Z

← Older revision		Revision as of 07:01, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PCY1A_RAT PCY1A_RAT]] Controls phosphatidylcholine synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:07, 8 December 2014

2014-12-08T09:07:16Z

← Older revision		Revision as of 09:07, 8 December 2014
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	[[Image:~~3hl4~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline==
			<StructureSection load='3hl4' size='340' side='right' caption='[[3hl4]], [[Resolution\|resolution]] 2.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3hl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HL4 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CDC:[2-CYTIDYLATE-O-PHOSPHONYLOXYL]-ETHYL-TRIMETHYL-AMMONIUM'>CDC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ctpct, Pcyt1, Pcyt1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline-phosphate_cytidylyltransferase Choline-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.15 2.7.7.15] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hl4 PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hl4_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			CTP:phosphocholine cytidylyltransferase (CCT) is the key regulatory enzyme in the synthesis of phosphatidylcholine, the most abundant phospholipid in eukaryotic cell membranes. The CCT-catalyzed transfer of a cytidylyl group from CTP to phosphocholine to form CDP-choline is regulated by a membrane lipid-dependent mechanism imparted by its C-terminal membrane binding domain. We present the first analysis of a crystal structure of a eukaryotic CCT. A deletion construct of rat CCTalpha spanning residues 1-236 (CCT236) lacks the regulatory domain and as a result displays constitutive activity. The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline. Each chain is composed of a complete catalytic domain with an intimately associated N-terminal extension, which together with the catalytic domain contributes to the dimer interface. Although the CCT236 structure reveals elements involved in binding cytidine that are conserved with other members of the cytidylyltransferase superfamily, it also features nonconserved active site residues, His-168 and Tyr-173, that make key interactions with the beta-phosphate of CDP-choline. Mutagenesis and kinetic analyses confirmed their role in phosphocholine binding and catalysis. These results demonstrate structural and mechanistic differences in a broadly conserved protein fold across the cytidylyltransferase family. Comparison of the CCT236 structure with those of other nucleotidyltransferases provides evidence for substrate-induced active site loop movements and a disorder-to-order transition of a loop element in the catalytic mechanism.

	~~{{STRUCTURE_3hl4\| PDB=3hl4 \| SCENE= }}~~		Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.,Lee J, Johnson J, Ding Z, Paetzel M, Cornell RB J Biol Chem. 2009 Nov 27;284(48):33535-48. Epub 2009 Sep 25. PMID:19783652<ref>PMID:19783652</ref>

	~~===Crystal structure~~ of ~~a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19783652}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[3hl4]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rattus_norvegicus Rattus norvegicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019783652<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Choline-phosphate cytidylyltransferase]]		[[Category: Choline-phosphate cytidylyltransferase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Cornell, R B.]]		[[Category: Cornell, R B]]
	[[Category: Lee, J.]]		[[Category: Lee, J]]
	[[Category: Paetzel, M.]]		[[Category: Paetzel, M]]
	[[Category: Amphitropic protein]]		[[Category: Amphitropic protein]]
	[[Category: Cdp-choline]]		[[Category: Cdp-choline]]

OCA: Protected "3hl4" [edit=sysop:move=sysop]

2013-03-06T15:01:16Z

Protected "3hl4" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:01, 6 March 2013
(No difference)

OCA at 15:01, 6 March 2013

2013-03-06T15:01:14Z

← Older revision		Revision as of 15:01, 6 March 2013
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	~~{{Seed}}~~
	[[Image:3hl4.png\|left\|200px]]		[[Image:3hl4.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3hl4", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3hl4\| PDB=3hl4 \| SCENE= }}		{{STRUCTURE_3hl4\| PDB=3hl4 \| SCENE= }}

	===Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===		===Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19783652}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19783652 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19783652}}		{{ABSTRACT_PUBMED_19783652}}

	==About this Structure==		==About this Structure==
	~~3HL4~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA].		[[3hl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19783652~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019783652</ref><references group="xtra"/>
	[[Category: Choline-phosphate cytidylyltransferase]]		[[Category: Choline-phosphate cytidylyltransferase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
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	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 09:03:42 2009''~~

OCA at 07:03, 2 December 2009

2009-12-02T07:03:43Z

← Older revision		Revision as of 07:03, 2 December 2009
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	{{Seed}}		{{Seed}}
	[[Image:3hl4.~~jpg~~\|left\|200px]]		[[Image:3hl4.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===		===Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_19783652}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 19783652 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_19783652}}

	==About this Structure==		==About this Structure==
	3HL4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA].		3HL4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL4 OCA].

			==Reference==
			<ref group="xtra">PMID:19783652</ref><references group="xtra"/>
	[[Category: Choline-phosphate cytidylyltransferase]]		[[Category: Choline-phosphate cytidylyltransferase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
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	[[Category: Transferase]]		[[Category: Transferase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 23 08~~:58:11 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 09:03:42 2009''

OCA at 05:58, 23 September 2009

2009-09-23T05:58:12Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3hl4 is ON HOLD  until Paper Publication
+<!--
-Authors: Lee, J., Paetzel, M., Cornell, R.B.
+===Crystal structure of a mammalian CTP:phosphocholine cytidylyltransferase with CDP-choline===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:10:50 2009''
+==About this Structure==