3hlx - Revision history

OCA at 15:50, 1 November 2023

2023-11-01T15:50:36Z

← Older revision		Revision as of 15:50, 1 November 2023
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	<StructureSection load='3hlx' size='340' side='right'caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3hlx' size='340' side='right'caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Klep7 Klep7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_MGH_78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HLX FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~pqqC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=272620 KLEP7])</td~~></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [https://pdbe.org/3hlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [https://www.ebi.ac.uk/pdbsum/3hlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [https://pdbe.org/3hlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [https://www.ebi.ac.uk/pdbsum/3hlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PQQC_KLEP7 PQQC_KLEP7]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.[HAMAP-Rule:MF_00654]		[https://www.uniprot.org/uniprot/PQQC_KLEP7 PQQC_KLEP7] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.[HAMAP-Rule:MF_00654]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Klep7~~]]		[[Category: Klebsiella pneumoniae subsp. pneumoniae MGH 78578]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Pyrroloquinoline-quinone synthase]]~~		[[Category: Puehringer S]]
	[[Category: Puehringer, S]]		[[Category: Schwarzenbacher R]]
	[[Category: Schwarzenbacher, R]]
	~~[[Category: All helical]]~~
	~~[[Category: Complex]]~~
	~~[[Category: Oxidase]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Pqq biosynthesis]]~~
	~~[[Category: Pqqc~~]]

OCA at 12:51, 23 March 2022

2022-03-23T12:51:51Z

← Older revision		Revision as of 12:51, 23 March 2022
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	==Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ==		==Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ==
	<StructureSection load='3hlx' size='340' side='right' caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3hlx' size='340' side='right'caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3HLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HLX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqqC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqqC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [~~http~~://pdbe.org/3hlx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3hlx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [https://pdbe.org/3hlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [https://www.ebi.ac.uk/pdbsum/3hlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PQQC_KLEP7 PQQC_KLEP7]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.[HAMAP-Rule:MF_00654]		[[https://www.uniprot.org/uniprot/PQQC_KLEP7 PQQC_KLEP7]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.[HAMAP-Rule:MF_00654]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Klep7]]		[[Category: Klep7]]
			[[Category: Large Structures]]
	[[Category: Pyrroloquinoline-quinone synthase]]		[[Category: Pyrroloquinoline-quinone synthase]]
	[[Category: Puehringer, S]]		[[Category: Puehringer, S]]

OCA at 08:07, 12 December 2018

2018-12-12T08:07:44Z

← Older revision		Revision as of 08:07, 12 December 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hlx_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hlx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:04, 5 August 2016

2016-08-05T06:04:19Z

← Older revision		Revision as of 06:04, 5 August 2016
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	==Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ==		==Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ==
	<StructureSection load='3hlx' size='340' side='right' caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3hlx' size='340' side='right' caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HLX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HLX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqqC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~72407 Klebsiella pneumoniae subsp. pneumoniae~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqqC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [http://www.ebi.ac.uk/pdbsum/3hlx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [http://pdbe.org/3hlx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [http://www.ebi.ac.uk/pdbsum/3hlx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hlx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3hlx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Klebsiella pneumoniae subsp. pneumoniae~~]]		[[Category: Klep7]]
	[[Category: Pyrroloquinoline-quinone synthase]]		[[Category: Pyrroloquinoline-quinone synthase]]
	[[Category: Puehringer, S]]		[[Category: Puehringer, S]]

OCA at 16:22, 25 December 2014

2014-12-25T16:22:56Z

← Older revision		Revision as of 16:22, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [http://www.ebi.ac.uk/pdbsum/3hlx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [http://www.ebi.ac.uk/pdbsum/3hlx PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PQQC_KLEP7 PQQC_KLEP7]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.[HAMAP-Rule:MF_00654]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:14, 18 December 2014

2014-12-18T06:14:46Z

← Older revision		Revision as of 06:14, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3hlx\| PDB=3hlx \| SCENE= }}~~		==Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ==
	===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===		<StructureSection load='3hlx' size='340' side='right' caption='[[3hlx]], [[Resolution\|resolution]] 1.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_20602352}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3hlx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HLX FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1otw\|1otw]], [[1otv\|1otv]], [[3hml\|3hml]], [[3hnh\|3hnh]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqqC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=72407 Klebsiella pneumoniae subsp. pneumoniae])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrroloquinoline-quinone_synthase Pyrroloquinoline-quinone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.11 1.3.3.11] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hlx RCSB], [http://www.ebi.ac.uk/pdbsum/3hlx PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hlx_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pyrroloquinoline quinone [4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid (PQQ)] is a bacterial cofactor in numerous alcohol dehydrogenases including methanol dehydrogenase and glucose dehydrogenase. Its biosynthesis in Klebsiella pneumoniae is facilitated by six genes, pqqABCDEF and proceeds by an unknown pathway. PqqC is one of two metal free oxidases of known structure and catalyzes the last step of PQQ biogenesis which involves a ring closure and an eight-electron oxidation of the substrate [3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9- dicarboxylic acid (AHQQ)]. PqqC has 14 conserved active site residues, which have previously been shown to be in close contact with bound PQQ. Herein, we describe the structures of three PqqC active site variants, H154S, Y175F, and the double mutant R179S/Y175S. The H154S crystal structure shows that, even with PQQ bound, the enzyme is still in the "open" conformation with helices alpha5b and alpha6 unfolded and the active site solvent accessible. The Y175F PQQ complex crystal structure reveals the closed conformation indicating that Y175 is not required for the conformational change. The R179S/Y175S AHQQ complex crystal structure is the most mechanistically informative, indicating an open conformation with a reaction intermediate trapped in the active site. The intermediate seen in R179S/Y175S is tricyclic but nonplanar, implying that it has not undergone oxidation. These studies implicate a stepwise process in which substrate binding leads to the generation of the closed protein conformation, with the latter playing a critical role in O(2) binding and catalysis. Proteins 2010. (c) 2010 Wiley-Liss, Inc.

	~~==About this Structure==~~		Structural studies of mutant forms of the PQQ-forming enzyme PqqC in the presence of product and substrate.,Puehringer S, Rosefigura J, Metlitzky M, Toyama H, Klinman JP, Schwarzenbacher R Proteins. 2010 Aug 15;78(11):2554-62. PMID:20602352<ref>PMID:20602352</ref>
	~~[[3hlx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Klebsiella_pneumoniae_subsp~~.~~_pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	<~~ref group="xtra">PMID:020602352<~~/~~ref~~><~~references group="xtra"~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Klebsiella pneumoniae subsp. pneumoniae]]		[[Category: Klebsiella pneumoniae subsp. pneumoniae]]
	[[Category: Pyrroloquinoline-quinone synthase]]		[[Category: Pyrroloquinoline-quinone synthase]]
	[[Category: Puehringer, S.]]		[[Category: Puehringer, S]]
	[[Category: Schwarzenbacher, R.]]		[[Category: Schwarzenbacher, R]]
	[[Category: All helical]]		[[Category: All helical]]
	[[Category: Complex]]		[[Category: Complex]]

OCA: Protected "3hlx" [edit=sysop:move=sysop]

2013-03-13T10:50:37Z

Protected "3hlx" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:50, 13 March 2013
(No difference)

OCA at 10:50, 13 March 2013

2013-03-13T10:50:34Z

← Older revision		Revision as of 10:50, 13 March 2013
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	~~{{Seed}}~~
	~~[[Image:3hlx.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3hlx", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3hlx\| PDB=3hlx \| SCENE= }}		{{STRUCTURE_3hlx\| PDB=3hlx \| SCENE= }}

	===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===		===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20602352}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20602352 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20602352}}		{{ABSTRACT_PUBMED_20602352}}

	==About this Structure==		==About this Structure==
	~~3HLX~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA].		[[3hlx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20602352~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020602352</ref><references group="xtra"/>
	[[Category: Klebsiella pneumoniae subsp. pneumoniae]]		[[Category: Klebsiella pneumoniae subsp. pneumoniae]]
	[[Category: Pyrroloquinoline-quinone synthase]]		[[Category: Pyrroloquinoline-quinone synthase]]
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	[[Category: Pqq biosynthesis]]		[[Category: Pqq biosynthesis]]
	[[Category: Pqqc]]		[[Category: Pqqc]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:54:39 2010''~~

OCA at 11:48, 14 July 2010

2010-07-14T11:48:30Z

← Older revision		Revision as of 11:48, 14 July 2010
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	{{Seed}}		{{Seed}}
	[[Image:3hlx.~~jpg~~\|left\|200px]]		[[Image:3hlx.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===		===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_20602352}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 20602352 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_20602352}}

	==About this Structure==		==About this Structure==
	3HLX is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp.~~_pneumoniae_mgh_78578~~ Klebsiella pneumoniae subsp. pneumoniae ~~mgh 78578~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA].		3HLX is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLX OCA].
	[[Category: Klebsiella pneumoniae subsp. pneumoniae ~~mgh 78578~~]]
			==Reference==
			<ref group="xtra">PMID:20602352</ref><references group="xtra"/>
			[[Category: Klebsiella pneumoniae subsp. pneumoniae]]
	[[Category: Pyrroloquinoline-quinone synthase]]		[[Category: Pyrroloquinoline-quinone synthase]]
	[[Category: Puehringer, S.]]		[[Category: Puehringer, S.]]
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	[[Category: Pqqc]]		[[Category: Pqqc]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~May 12 11~~:14:58 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:54:39 2010''

OCA at 08:15, 12 May 2010

2010-05-12T08:15:00Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3hlx is ON HOLD  until Paper Publication
+<!--
-Authors: Puehringer, S., Schwarzenbacher, R.
+===Crystal Structure of PqqC Active Site Mutant Y175F in Complex with PQQ===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  1 08:56:06 2009''
+==About this Structure==