3it2 - Revision history

OCA at 07:57, 6 September 2023

2023-09-06T07:57:33Z

← Older revision		Revision as of 07:57, 6 September 2023
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	<StructureSection load='3it2' size='340' side='right'caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3it2' size='340' side='right'caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Frath Frath~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica_LVS Francisella tularensis subsp. holarctica LVS]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.838Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat~~">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3it0\|3it0]]~~, [[~~3it1~~\|~~3it1]], [[3it3\|3it3~~]]~~</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FTL_0031 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~376619 FRATH])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [https://pdbe.org/3it2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [https://www.ebi.ac.uk/pdbsum/3it2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3it2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [https://pdbe.org/3it2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [https://www.ebi.ac.uk/pdbsum/3it2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3it2 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Acid phosphatase]]~~		[[Category: Francisella tularensis subsp. holarctica LVS]]
	~~[[Category: Frath~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Felts~~, R L~~]]		[[Category: Felts RL]]
	[[Category: Reilly~~, T J~~]]		[[Category: Reilly TJ]]
	[[Category: Singh, H]]		[[Category: Singh H]]
	[[Category: Tanner~~, J J]]~~		[[Category: Tanner JJ]]
	~~[[Category: Hap]]~~
	~~[[Category: Histidine acid phosphatase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 03:41, 21 April 2022

2022-04-21T03:41:09Z

← Older revision		Revision as of 03:41, 21 April 2022
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	==Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase==		==Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase==
	<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3it2' size='340' side='right'caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Frath Frath]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Frath Frath]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376619 FRATH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376619 FRATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [~~http~~://pdbe.org/3it2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3it2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3it2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [https://pdbe.org/3it2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [https://www.ebi.ac.uk/pdbsum/3it2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3it2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Acid phosphatase\|Acid phosphatase]]		*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Frath]]		[[Category: Frath]]
			[[Category: Large Structures]]
	[[Category: Felts, R L]]		[[Category: Felts, R L]]
	[[Category: Reilly, T J]]		[[Category: Reilly, T J]]

OCA at 10:13, 19 December 2018

2018-12-19T10:13:55Z

← Older revision		Revision as of 10:13, 19 December 2018
Line 1:		Line 1:

	==Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase==		==Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase==
	<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376619 FRATH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376619 FRATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://pdbe.org/3it2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://pdbe.org/3it2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3it2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3it2_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3it2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 39:
	[[Category: Frath]]		[[Category: Frath]]
	[[Category: Felts, R L]]		[[Category: Felts, R L]]
	[[Category: Reilly,T J]]		[[Category: Reilly, T J]]
	[[Category: Singh, H]]		[[Category: Singh, H]]
	[[Category: Tanner, J J]]		[[Category: Tanner, J J]]

OCA at 12:19, 8 February 2016

2016-02-08T12:19:41Z

← Older revision		Revision as of 12:19, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Frath Frath]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~119857 Francisella tularensis subsp. holarctica~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376619 FRATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://pdbe.org/3it2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3it2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3it2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: ~~Francisella tularensis subsp. holarctica~~]]		[[Category: Frath]]
	[[Category: Felts, R L]]		[[Category: Felts, R L]]
	[[Category: Reilly,T J]]		[[Category: Reilly,T J]]

OCA at 09:52, 20 January 2015

2015-01-20T09:52:22Z

← Older revision		Revision as of 09:52, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119857 Francisella tularensis subsp. holarctica])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119857 Francisella tularensis subsp. holarctica])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Francisella tularensis subsp. holarctica]]		[[Category: Francisella tularensis subsp. holarctica]]
	[[Category: Felts, R L.]]		[[Category: Felts, R L]]
	[[Category: Reilly,T J.]]		[[Category: Reilly,T J]]
	[[Category: Singh, H.]]		[[Category: Singh, H]]
	[[Category: Tanner, J J.]]		[[Category: Tanner, J J]]
	[[Category: Hap]]		[[Category: Hap]]
	[[Category: Histidine acid phosphatase]]		[[Category: Histidine acid phosphatase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:39, 29 September 2014

2014-09-29T13:39:54Z

← Older revision		Revision as of 13:39, 29 September 2014
Line 1:		Line 1:
	[[Image:~~3it2~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase==
			<StructureSection load='3it2' size='340' side='right' caption='[[3it2]], [[Resolution\|resolution]] 1.84Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IT2 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3it0\|3it0]], [[3it1\|3it1]], [[3it3\|3it3]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTL_0031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119857 Francisella tularensis subsp. holarctica])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3it2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3it2 RCSB], [http://www.ebi.ac.uk/pdbsum/3it2 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3it2_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Histidine acid phosphatases catalyze the transfer of a phosphoryl group from phosphomonoesters to water at acidic pH using an active-site histidine. The histidine acid phosphatase from the category A pathogen Francisella tularensis (FtHAP) has been implicated in intramacrophage survival and virulence, motivating interest in understanding the structure and mechanism of this enzyme. Here, we report a structure-based study of ligand recognition by FtHAP. The 1.70-A-resolution structure of FtHAP complexed with the competitive inhibitor l(+)-tartrate was solved using single-wavelength anomalous diffraction phasing. Structures of the ligand-free enzyme and the complex with inorganic phosphate were determined at resolutions of 1.85 and 1.70 A, respectively. The structure of the Asp261Ala mutant enzyme complexed with the substrate 3'-AMP was determined at 1.50 A resolution to gain insight into substrate recognition. FtHAP exhibits a two-domain fold similar to that of human prostatic acid phosphatase, consisting of an alpha/beta core domain and a smaller domain that caps the core domain. The structures show that the core domain supplies the phosphoryl binding site, catalytic histidine (His17), and an aspartic acid residue (Asp261) that protonates the leaving group, while the cap domain contributes residues that enforce substrate preference. FtHAP and human prostatic acid phosphatase differ in the orientation of the crucial first helix of the cap domain, implying differences in the substrate preferences of the two enzymes. 3'-AMP binds in one end of a 15-A-long tunnel, with the adenine clamped between Phe23 and Tyr135, and the ribose 2'-hydroxyl interacting with Gln132. The importance of the clamp is confirmed with site-directed mutagenesis; mutation of Phe23 and Tyr135 individually to Ala increases K(m) by factors of 7 and 10, respectively. The structural data are consistent with a role for FtHAP in scavenging phosphate from small molecules present in host macrophage cells.

	~~{{STRUCTURE_3it2\| PDB=3it2 \| SCENE= }}~~		Crystal Structures of the histidine acid phosphatase from Francisella tularensis provide insight into substrate recognition.,Singh H, Felts RL, Schuermann JP, Reilly TJ, Tanner JJ J Mol Biol. 2009 Dec 18;394(5):893-904. Epub 2009 Oct 21. PMID:19836403<ref>PMID:19836403</ref>

	~~===Crystal structure~~ of ~~ligand-free Francisella tularensis histidine acid phosphatase===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19836403}}~~

	~~==About this Structure==~~
	~~[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].~~

	==See Also==		==See Also==
	*[[Acid phosphatase\|Acid phosphatase]]		*[[Acid phosphatase\|Acid phosphatase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:019836403<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Francisella tularensis subsp. holarctica]]		[[Category: Francisella tularensis subsp. holarctica]]

OCA at 10:06, 20 October 2012

2012-10-20T10:06:58Z

← Older revision		Revision as of 10:06, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3it2]] is a 2 chain structure ~~of [[Acid phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].		[[3it2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].

	==See Also==		==See Also==

OCA: Protected "3it2" [edit=sysop:move=sysop]

2012-07-26T01:01:54Z

Protected "3it2" [edit=sysop:move=sysop]

← Older revision	Revision as of 01:01, 26 July 2012
(No difference)

OCA at 01:01, 26 July 2012

2012-07-26T01:01:53Z

← Older revision		Revision as of 01:01, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3it2.png\|left\|200px]]		[[Image:3it2.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3it2", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3it2\| PDB=3it2 \| SCENE= }}		{{STRUCTURE_3it2\| PDB=3it2 \| SCENE= }}

	===Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase===		===Crystal structure of ligand-free Francisella tularensis histidine acid phosphatase===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19836403}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 19836403 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_19836403}}		{{ABSTRACT_PUBMED_19836403}}

	==About this Structure==		==About this Structure==
	~~3IT2~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp.~~_holarctica_lvs~~ Francisella tularensis subsp. holarctica ~~lvs~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].		[[3it2]] is a 2 chain structure of [[Acid phosphatase]] with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica Francisella tularensis subsp. holarctica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].

			==See Also==
			*[[Acid phosphatase\|Acid phosphatase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19836403~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019836403</ref><references group="xtra"/>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Francisella tularensis subsp. holarctica ~~lvs~~]]		[[Category: Francisella tularensis subsp. holarctica]]
	[[Category: Felts, R L.]]		[[Category: Felts, R L.]]
	[[Category: Reilly,T J.]]		[[Category: Reilly,T J.]]
Line 33:		Line 24:
	[[Category: Histidine acid phosphatase]]		[[Category: Histidine acid phosphatase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 16:39:18 2010''~~

OCA at 14:39, 20 January 2010

2010-01-20T14:39:20Z

← Older revision		Revision as of 14:39, 20 January 2010
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	3IT2 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica_lvs Francisella tularensis subsp. holarctica lvs]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].		3IT2 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._holarctica_lvs Francisella tularensis subsp. holarctica lvs]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2glb 2glb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT2 OCA].

	==Reference==		==Reference==
Line 34:		Line 34:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 2 09~~:15:~~28 2009~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 16:39:18 2010''