3ixt - Revision history

OCA at 06:21, 27 November 2024

2024-11-27T06:21:41Z

← Older revision		Revision as of 06:21, 27 November 2024
Line 15:		Line 15:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 08:00, 6 September 2023

2023-09-06T08:00:08Z

← Older revision		Revision as of 08:00, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3ixt' size='340' side='right'caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3ixt' size='340' side='right'caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human_respiratory_syncytial_virus~~ Human respiratory syncytial virus] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3IXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_A2 Human respiratory syncytial virus A2] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [~~http~~://pdbe.org/3ixt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [https://pdbe.org/3ixt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [https://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/FUS_HRSVA FUS_HRSVA]] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.<ref>PMID:12663767</ref> <ref>PMID:18216092</ref>		[https://www.uniprot.org/uniprot/FUS_HRSVA FUS_HRSVA] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.<ref>PMID:12663767</ref> <ref>PMID:18216092</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Human respiratory syncytial virus]]		[[Category: Human respiratory syncytial virus A2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Chen, M]]		[[Category: Chen M]]
	[[Category: Graham~~, B S~~]]		[[Category: Graham BS]]
	[[Category: Kim, A]]		[[Category: Kim A]]
	[[Category: Kwong~~, P D~~]]		[[Category: Kwong PD]]
	[[Category: McLellan~~, J S~~]]		[[Category: McLellan JS]]
	[[Category: Yang, Y]]		[[Category: Yang Y]]
	~~[[Category: Cell membrane]]~~
	~~[[Category: Cleavage on pair of basic residue]]~~
	~~[[Category: Complex]]~~
	~~[[Category: Disulfide bond]]~~
	~~[[Category: Envelope protein]]~~
	~~[[Category: Fab]]~~
	~~[[Category: Fusion protein]]~~
	~~[[Category: Glycoprotein]]~~
	~~[[Category: Immune system]]~~
	~~[[Category: Lipoprotein]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Monoclonal]]~~
	~~[[Category: Motavizumab]]~~
	~~[[Category: Palmitate]]~~
	~~[[Category: Rsv]]~~
	~~[[Category: Synagi]]~~
	~~[[Category: Transmembrane]]~~
	~~[[Category: Virion~~]]

OCA at 11:59, 23 September 2020

2020-09-23T11:59:17Z

← Older revision		Revision as of 11:59, 23 September 2020
Line 1:		Line 1:

	==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==		==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==
	<StructureSection load='3ixt' size='340' side='right' caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3ixt' size='340' side='right'caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://pdbe.org/3ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://pdbe.org/3ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human respiratory syncytial virus]]		[[Category: Human respiratory syncytial virus]]
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Chen, M]]		[[Category: Chen, M]]

OCA at 09:28, 19 December 2018

2018-12-19T09:28:33Z

← Older revision		Revision as of 09:28, 19 December 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:

	==See Also==		==See Also==
	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]		*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:27, 4 August 2016

2016-08-04T21:27:36Z

← Older revision		Revision as of 21:27, 4 August 2016
Line 1:		Line 1:

	==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==		==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==
	<StructureSection load='3ixt' size='340' side='right' caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3ixt' size='340' side='right' caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://pdbe.org/3ixt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ixt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3ixt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[~~Monoclonal Antibody~~\|~~Monoclonal Antibody~~]]		*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human respiratory syncytial virus]]		[[Category: Human respiratory syncytial virus]]
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Chen, M]]		[[Category: Chen, M]]
	[[Category: Graham, B S]]		[[Category: Graham, B S]]

OCA at 13:17, 25 December 2014

2014-12-25T13:17:07Z

← Older revision		Revision as of 13:17, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/FUS_HRSVA FUS_HRSVA]] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.<ref>PMID:12663767</ref> <ref>PMID:18216092</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Human respiratory syncytial virus]]		[[Category: Human respiratory syncytial virus]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Chen, M.]]		[[Category: Chen, M]]
	[[Category: Graham, B S.]]		[[Category: Graham, B S]]
	[[Category: Kim, A.]]		[[Category: Kim, A]]
	[[Category: Kwong, P D.]]		[[Category: Kwong, P D]]
	[[Category: McLellan, J S.]]		[[Category: McLellan, J S]]
	[[Category: Yang, Y.]]		[[Category: Yang, Y]]
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]

OCA at 10:55, 29 September 2014

2014-09-29T10:55:41Z

← Older revision		Revision as of 10:55, 29 September 2014
Line 1:		Line 1:
	[[Image:~~3ixt~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==
			<StructureSection load='3ixt' size='340' side='right' caption='[[3ixt]], [[Resolution\|resolution]] 2.75Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [http://www.ebi.ac.uk/pdbsum/3ixt PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Motavizumab is approximately tenfold more potent than its predecessor, palivizumab (Synagis), the FDA-approved monoclonal antibody used to prevent respiratory syncytial virus (RSV) infection. The structure of motavizumab in complex with a 24-residue peptide corresponding to its epitope on the RSV fusion (F) glycoprotein reveals the structural basis for this greater potency. Modeling suggests that motavizumab recognizes a different quaternary configuration of the F glycoprotein than that observed in a homologous structure.

	~~{{STRUCTURE_3ixt\| PDB=3ixt \| SCENE= }}~~		Structural basis of respiratory syncytial virus neutralization by motavizumab.,McLellan JS, Chen M, Kim A, Yang Y, Graham BS, Kwong PD Nat Struct Mol Biol. 2010 Feb;17(2):248-50. Epub 2010 Jan 24. PMID:20098425<ref>PMID:20098425</ref>

	~~===Crystal Structure~~ of ~~Motavizumab Fab Bound to Peptide Epitope===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_20098425}}~~

	~~==About this Structure==~~
	~~[[3ixt]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en~~.~~wikipedia.org~~/~~wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].~~

	==See Also==		==See Also==
	*[[Monoclonal Antibody\|Monoclonal Antibody]]		*[[Monoclonal Antibody\|Monoclonal Antibody]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:020098425<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Human respiratory syncytial virus]]		[[Category: Human respiratory syncytial virus]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 17:08, 20 October 2012

2012-10-20T17:08:21Z

← Older revision		Revision as of 17:08, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3ixt]] is a 6 chain structure ~~of [[Monoclonal Antibody]]~~ with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].		[[3ixt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].

	==See Also==		==See Also==

OCA at 23:57, 26 July 2012

2012-07-26T23:57:11Z

← Older revision		Revision as of 23:57, 26 July 2012
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	[[Image:3ixt.png\|left\|200px]]		[[Image:3ixt.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3ixt", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3ixt\| PDB=3ixt \| SCENE= }}		{{STRUCTURE_3ixt\| PDB=3ixt \| SCENE= }}

	===Crystal Structure of Motavizumab Fab Bound to Peptide Epitope===		===Crystal Structure of Motavizumab Fab Bound to Peptide Epitope===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20098425}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20098425 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20098425}}		{{ABSTRACT_PUBMED_20098425}}

	==About this Structure==		==About this Structure==
	[[3ixt]] is a 6 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/~~Human_respiratory_syncytial_virus_a2~~ Human respiratory syncytial virus a2] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].		[[3ixt]] is a 6 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].

	==See Also==		==See Also==
	*[[Monoclonal Antibody]]		*[[Monoclonal Antibody\|Monoclonal Antibody]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20098425~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020098425</ref><references group="xtra"/>
	[[Category: Human respiratory syncytial virus a2]]		[[Category: Human respiratory syncytial virus]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Chen, M.]]		[[Category: Chen, M.]]
Line 36:		Line 25:
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
	~~[[Category: Coiled coil]]~~
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]

OCA: Protected "3ixt" [edit=sysop:move=sysop]

2010-12-26T23:47:45Z

Protected "3ixt" [edit=sysop:move=sysop]

← Older revision	Revision as of 23:47, 26 December 2010
(No difference)