3kl4 - Revision history

OCA at 10:17, 21 February 2024

2024-02-21T10:17:21Z

← Older revision		Revision as of 10:17, 21 February 2024
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	==Recognition of a signal peptide by the signal recognition particle==		==Recognition of a signal peptide by the signal recognition particle==
	<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='3kl4' size='340' side='right'caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [~~http~~://en.wikipedia.org/wiki/~~Sacs2 Sacs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih~~.~~gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=273057 SACS2])</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [https://pdbe.org/3kl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [https://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [~~http~~://pdbe.org/3kl4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~SRP54_SULSO SRP54_SULSO]~~] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY ~~(By similarity)~~.		[https://www.uniprot.org/uniprot/SRP54_SACS2 SRP54_SACS2] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.[HAMAP-Rule:MF_00306]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kl4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kl4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Targeting of proteins to appropriate subcellular compartments is a crucial process in all living cells. Secretory and membrane proteins usually contain an amino-terminal signal peptide, which is recognized by the signal recognition particle (SRP) when nascent polypeptide chains emerge from the ribosome. The SRP-ribosome nascent chain complex is then targeted through its GTP-dependent interaction with SRP receptor to the protein-conducting channel on endoplasmic reticulum membrane in eukaryotes or plasma membrane in bacteria. A universally conserved component of SRP (refs 1, 2), SRP54 or its bacterial homologue, fifty-four homologue (Ffh), binds the signal peptides, which have a highly divergent sequence divisible into a positively charged n-region, an h-region commonly containing 8-20 hydrophobic residues and a polar c-region. No structure has been reported that exemplifies SRP54 binding of any signal sequence. Here we have produced a fusion protein between Sulfolobus solfataricus SRP54 (Ffh) and a signal peptide connected via a flexible linker. This fusion protein oligomerizes in solution through interaction between the SRP54 and signal peptide moieties belonging to different chains, and it is functional, as demonstrated by its ability to bind SRP RNA and SRP receptor FtsY. We present the crystal structure at 3.5 A resolution of an SRP54-signal peptide complex in the dimer, which reveals how a signal sequence is recognized by SRP54.

	Recognition of a signal peptide by the signal recognition particle.,Janda CY, Li J, Oubridge C, Hernandez H, Robinson CV, Nagai K Nature. 2010 May 27;465(7297):507-10. Epub 2010 Apr 4. PMID:20364120<ref>PMID:20364120</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3kl4" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Signal recognition particle ~~protein~~\|Signal recognition particle ~~protein~~]]		*[[Signal recognition particle 3D structures\|Signal recognition particle 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 18824~~]]		[[Category: Large Structures]]
	[[Category: ~~Sacs2~~]]		[[Category: Saccharolobus solfataricus P2]]
	[[Category: ~~Signal-recognition-particle GTPase~~]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Janda~~, C Y~~]]		[[Category: Janda CY]]
	[[Category: Li, J]]		[[Category: Li J]]
	[[Category: Nagai, K]]		[[Category: Nagai K]]
	[[Category: Oubridge, C]]		[[Category: Oubridge C]]
	~~[[Category: Ffh]]~~
	~~[[Category: Gtp-binding]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Nucleotide-binding]]~~
	~~[[Category: Ribonucleoprotein]]~~
	~~[[Category: Rna-binding]]~~
	~~[[Category: Signal peptide]]~~
	~~[[Category: Signal recognition particle]]~~
	~~[[Category: Signal sequence]]~~
	~~[[Category: Signal-anchor]]~~
	~~[[Category: Srp]]~~
	~~[[Category: Srp54]]~~
	~~[[Category: Transmembrane~~]]

OCA at 09:01, 26 December 2018

2018-12-26T09:01:18Z

← Older revision		Revision as of 09:01, 26 December 2018
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	<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/~~Sulso Sulso~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 ~~SULSO~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://pdbe.org/3kl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://pdbe.org/3kl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/3kl4_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/3kl4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
			[[Category: Sacs2]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
	~~[[Category: Sulso]]~~
	[[Category: Janda, C Y]]		[[Category: Janda, C Y]]
	[[Category: Li, J]]		[[Category: Li, J]]

OCA at 09:50, 4 August 2016

2016-08-04T09:50:31Z

← Older revision		Revision as of 09:50, 4 August 2016
Line 1:		Line 1:

	==Recognition of a signal peptide by the signal recognition particle==		==Recognition of a signal peptide by the signal recognition particle==
	<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~] and [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 ~~Sulfolobus solfataricus P2~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://pdbe.org/3kl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kl4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3kl4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Saccharomyces cerevisiae~~]]		[[Category: Atcc 18824]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
	[[Category: ~~Sulfolobus solfataricus p2~~]]		[[Category: Sulso]]
	[[Category: Janda, C Y]]		[[Category: Janda, C Y]]
	[[Category: Li, J]]		[[Category: Li, J]]

OCA at 08:03, 25 December 2014

2014-12-25T08:03:08Z

← Older revision		Revision as of 08:03, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/SRP54_SULSO SRP54_SULSO]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:40, 18 December 2014

2014-12-18T16:40:43Z

← Older revision		Revision as of 16:40, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3kl4~~\| ~~PDB~~=3kl4 \| ~~SCENE~~= }}		==Recognition of a signal peptide by the signal recognition particle==
	===~~Recognition~~ of a signal peptide by the signal recognition particle~~===~~		<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution\|resolution]] 3.50Å' scene=''>
	~~{{ABSTRACT_PUBMED_20364120}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ffh\|2ffh]], [[1qzx\|1qzx]], [[1qzw\|1qzw]], [[1dul\|1dul]], [[1j8m\|1j8m]], [[2j28\|2j28]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 Sulfolobus solfataricus P2])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/3kl4_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Targeting of proteins to appropriate subcellular compartments is a crucial process in all living cells. Secretory and membrane proteins usually contain an amino-terminal signal peptide, which is recognized by the signal recognition particle (SRP) when nascent polypeptide chains emerge from the ribosome. The SRP-ribosome nascent chain complex is then targeted through its GTP-dependent interaction with SRP receptor to the protein-conducting channel on endoplasmic reticulum membrane in eukaryotes or plasma membrane in bacteria. A universally conserved component of SRP (refs 1, 2), SRP54 or its bacterial homologue, fifty-four homologue (Ffh), binds the signal peptides, which have a highly divergent sequence divisible into a positively charged n-region, an h-region commonly containing 8-20 hydrophobic residues and a polar c-region. No structure has been reported that exemplifies SRP54 binding of any signal sequence. Here we have produced a fusion protein between Sulfolobus solfataricus SRP54 (Ffh) and a signal peptide connected via a flexible linker. This fusion protein oligomerizes in solution through interaction between the SRP54 and signal peptide moieties belonging to different chains, and it is functional, as demonstrated by its ability to bind SRP RNA and SRP receptor FtsY. We present the crystal structure at 3.5 A resolution of an SRP54-signal peptide complex in the dimer, which reveals how a signal sequence is recognized by SRP54.

	~~==Function==~~		Recognition of a signal peptide by the signal recognition particle.,Janda CY, Li J, Oubridge C, Hernandez H, Robinson CV, Nagai K Nature. 2010 May 27;465(7297):507-10. Epub 2010 Apr 4. PMID:20364120<ref>PMID:20364120</ref>
	~~[[http://www.uniprot.org/uniprot/SRP54_SULSO SRP54_SULSO]] Involved in targeting and insertion~~ of ~~nascent membrane proteins into~~ the ~~cytoplasmic membrane~~. ~~Binds to the hydrophobic signal sequence of the ribosome-nascent chain~~ (~~RNC~~) ~~as it emerges from the ribosomes~~. ~~The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity)~~.

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[3kl4]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=3KL4 OCA].~~		</div>

	==~~Reference~~==		==See Also==
	~~<ref group~~=~~"xtra">PMID:020364120</ref>~~<references ~~group="xtra"~~/><~~references~~/>		*[[Signal recognition particle protein\|Signal recognition particle protein]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
	[[Category: Sulfolobus solfataricus p2]]		[[Category: Sulfolobus solfataricus p2]]
	[[Category: Janda, C Y.]]		[[Category: Janda, C Y]]
	[[Category: Li, J.]]		[[Category: Li, J]]
	[[Category: Nagai, K.]]		[[Category: Nagai, K]]
	[[Category: Oubridge, C.]]		[[Category: Oubridge, C]]
	[[Category: Ffh]]		[[Category: Ffh]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]

OCA at 06:45, 29 September 2013

2013-09-29T06:45:54Z

← Older revision		Revision as of 06:45, 29 September 2013
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
	[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus~~ Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].		[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:020364120~~</ref><ref group="xtra">PMID:009695947</ref><ref group="xtra">PMID:014657338</ref><ref group="xtra">PMID:017086193~~</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:020364120</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus p2]]
	[[Category: Janda, C Y.]]		[[Category: Janda, C Y.]]
	[[Category: Li, J.]]		[[Category: Li, J.]]

OCA at 02:15, 4 April 2013

2013-04-04T02:15:54Z

← Older revision		Revision as of 02:15, 4 April 2013
Line 1:		Line 1:
	~~[[Image:3kl4.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3kl4", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3kl4\| PDB=3kl4 \| SCENE= }}		{{STRUCTURE_3kl4\| PDB=3kl4 \| SCENE= }}

	===Recognition of a signal peptide by the signal recognition particle===		===Recognition of a signal peptide by the signal recognition particle===
			{{ABSTRACT_PUBMED_20364120}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/SRP54_SULSO SRP54_SULSO]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20364120}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), where ~~20364120 is~~ the ~~PubMed ID number~~.
	~~-->~~
	~~{{ABSTRACT_PUBMED_20364120}}~~

	==About this Structure==		==About this Structure==
	[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus_p2~~ Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].		[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20364120~~</ref><ref group="xtra">PMID:~~9695947~~</ref><ref group="xtra">PMID:~~14657338~~</ref><ref group="xtra">PMID:~~10678824~~</ref><~~ref group="xtra">PMID:10801496</ref><ref~~ group="xtra"~~>PMID:17086193<~~/~~ref~~><references ~~group="xtra"~~/>		<ref group="xtra">PMID:020364120</ref><ref group="xtra">PMID:009695947</ref><ref group="xtra">PMID:014657338</ref><ref group="xtra">PMID:017086193</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
	[[Category: Sulfolobus solfataricus p2]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Janda, C Y.]]		[[Category: Janda, C Y.]]
	[[Category: Li, J.]]		[[Category: Li, J.]]

OCA: Protected "3kl4" [edit=sysop:move=sysop]

2011-03-14T21:30:35Z

Protected "3kl4" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:30, 14 March 2011
(No difference)

OCA at 21:30, 14 March 2011

2011-03-14T21:30:33Z

← Older revision		Revision as of 21:30, 14 March 2011
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:3kl4.png\|left\|200px]]		[[Image:3kl4.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
	~~3KL4~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].		[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA].

	==Reference==		==Reference==
Line 44:		Line 43:
	[[Category: Srp54]]		[[Category: Srp54]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 2 08:04:08 2010''~~

OCA at 04:00, 2 June 2010

2010-06-02T04:00:00Z

← Older revision		Revision as of 04:00, 2 June 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
	[[Image:3kl4.~~jpg~~\|left\|200px]]		[[Image:3kl4.png\|left\|200px]]

	<!--		<!--
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	===Recognition of a signal peptide by the signal recognition particle===		===Recognition of a signal peptide by the signal recognition particle===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_20364120}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 20364120 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_20364120}}

	==About this Structure==		==About this Structure==
Line 17:		Line 23:

	==Reference==		==Reference==
	<ref group="xtra">PMID:9695947</ref><ref group="xtra">PMID:14657338</ref><ref group="xtra">PMID:10678824</ref><ref group="xtra">PMID:10801496</ref><ref group="xtra">PMID:17086193</ref><references group="xtra"/>		<ref group="xtra">PMID:20364120</ref><ref group="xtra">PMID:9695947</ref><ref group="xtra">PMID:14657338</ref><ref group="xtra">PMID:10678824</ref><ref group="xtra">PMID:10801496</ref><ref group="xtra">PMID:17086193</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Signal-recognition-particle GTPase]]		[[Category: Signal-recognition-particle GTPase]]
Line 39:		Line 45:
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 31 13~~:51:24 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 2 08:04:08 2010''