3klk - Revision history

OCA at 08:45, 7 February 2024

2024-02-07T08:45:20Z

← Older revision		Revision as of 08:45, 7 February 2024
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	<StructureSection load='3klk' size='340' side='right'caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3klk' size='340' side='right'caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_23272 Atcc 23272~~]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KLK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri Limosilactobacillus reuteri]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KLK FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gtf180 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1598 ATCC 23272])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [https://pdbe.org/3klk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [https://www.ebi.ac.uk/pdbsum/3klk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3klk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [https://pdbe.org/3klk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [https://www.ebi.ac.uk/pdbsum/3klk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3klk ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q5SBN3_LIMRT Q5SBN3_LIMRT] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.[ARBA:ARBA00003243]
	~~Glucansucrases are large enzymes belonging to glycoside hydrolase family 70, which catalyze the cleavage of sucrose into fructose and glucose, with the concomitant transfer~~ of ~~the glucose residue to a growing alpha-glucan polymer. Among others, plaque-forming oral bacteria secrete these enzymes to produce alpha-~~glucans, ~~which facilitate the adhesion of the bacteria~~ to ~~the tooth enamel. We determined the crystal structure of~~ a ~~fully active, 1,031-residue fragment encompassing~~ the ~~catalytic and C-terminal domains~~ of ~~GTF180 from Lactobacillus reuteri 180, both in~~ the native state, and in complexes with sucrose and maltose. These structures show that the enzyme has an alpha-amylase-like (beta/alpha)(8)-barrel catalytic domain that is circularly permuted compared to the catalytic domains of ~~members of glycoside hydrolase families 13 and 77, which belong~~ to ~~the same GH-H superfamily. In contrast~~ to previous suggestions, the enzyme has only one active site and one nucleophilic residue. Surprisingly, in GTF180 the peptide chain follows a "U"-path, such that four of the five domains are made up from discontiguous N- and ~~C-terminal stretches of the peptide chain. Finally, the structures give insight into the factors that determine the different linkage types in~~ the ~~polymeric product.~~

	~~Crystal structure~~ of ~~a 117 kDa glucansucrase fragment provides insight into evolution~~ and ~~product specificity of GH70 enzymes.,Vujicic-Zagar A, Pijning T, Kralj S, Lopez CA, Eeuwema W, Dijkhuizen L, Dijkstra BW Proc Natl Acad Sci U S A. 2010 Nov 30. PMID:21118988<ref>PMID:21118988</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine~~.~~<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3klk" style="background-color~~:~~#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 23272]]~~
	~~[[Category: Dextransucrase]]~~
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Limosilactobacillus reuteri]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Dijkhuizen, L]]		[[Category: Dijkhuizen L]]
	[[Category: Dijkstra~~, B W~~]]		[[Category: Dijkstra BW]]
	[[Category: Eeuwema, W]]		[[Category: Eeuwema W]]
	[[Category: Kralj, S]]		[[Category: Kralj S]]
	[[Category: Pijning, T]]		[[Category: Pijning T]]
	[[Category: Vujicic-Zagar, A]]		[[Category: Vujicic-Zagar A]]
	~~[[Category: Glycosyltransferase]]~~
	~~[[Category: Multidomain protein]]~~
	~~[[Category: Native form]]~~
	~~[[Category: Open conformation]]~~
	~~[[Category: Transferase~~]]

OCA at 13:35, 4 May 2022

2022-05-04T13:35:43Z

← Older revision		Revision as of 13:35, 4 May 2022
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	==Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form==		==Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form==
	<StructureSection load='3klk' size='340' side='right' caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3klk' size='340' side='right'caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_23272 Atcc 23272]. The June 2011 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KLK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_23272 Atcc 23272]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KLK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf180 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1598 ATCC 23272])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf180 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1598 ATCC 23272])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [~~http~~://pdbe.org/3klk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3klk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3klk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [https://pdbe.org/3klk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [https://www.ebi.ac.uk/pdbsum/3klk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3klk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
			[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Dijkhuizen, L]]		[[Category: Dijkhuizen, L]]

OCA at 18:02, 11 August 2016

2016-08-11T18:02:18Z

← Older revision		Revision as of 18:02, 11 August 2016
Line 1:		Line 1:

	==Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form==		==Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form==
	<StructureSection load='3klk' size='340' side='right' caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3klk' size='340' side='right' caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactobacillus_reuteri Lactobacillus reuteri~~]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KLK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_23272 Atcc 23272]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KLK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1598 ~~Lactobacillus reuteri~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1598 ATCC 23272])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [http://www.ebi.ac.uk/pdbsum/3klk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [http://pdbe.org/3klk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [http://www.ebi.ac.uk/pdbsum/3klk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3klk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3klk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 23272]]
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	~~[[Category: Lactobacillus reuteri]]~~
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Dijkhuizen, L]]		[[Category: Dijkhuizen, L]]

OCA at 08:07, 20 January 2015

2015-01-20T08:07:33Z

← Older revision		Revision as of 08:07, 20 January 2015
Line 1:		Line 1:
	~~{{STRUCTURE_3klk\| PDB=3klk \| SCENE= }}~~		==Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form==
	===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===		<StructureSection load='3klk' size='340' side='right' caption='[[3klk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	~~{{ABSTRACT_PUBMED_21118988}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KLK FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3hq3\|3hq3]], [[3hz3\|3hz3]], [[3kll\|3kll]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1598 Lactobacillus reuteri])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3klk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3klk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3klk RCSB], [http://www.ebi.ac.uk/pdbsum/3klk PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glucansucrases are large enzymes belonging to glycoside hydrolase family 70, which catalyze the cleavage of sucrose into fructose and glucose, with the concomitant transfer of the glucose residue to a growing alpha-glucan polymer. Among others, plaque-forming oral bacteria secrete these enzymes to produce alpha-glucans, which facilitate the adhesion of the bacteria to the tooth enamel. We determined the crystal structure of a fully active, 1,031-residue fragment encompassing the catalytic and C-terminal domains of GTF180 from Lactobacillus reuteri 180, both in the native state, and in complexes with sucrose and maltose. These structures show that the enzyme has an alpha-amylase-like (beta/alpha)(8)-barrel catalytic domain that is circularly permuted compared to the catalytic domains of members of glycoside hydrolase families 13 and 77, which belong to the same GH-H superfamily. In contrast to previous suggestions, the enzyme has only one active site and one nucleophilic residue. Surprisingly, in GTF180 the peptide chain follows a "U"-path, such that four of the five domains are made up from discontiguous N- and C-terminal stretches of the peptide chain. Finally, the structures give insight into the factors that determine the different linkage types in the polymeric product.

	~~==About this Structure==~~		Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes.,Vujicic-Zagar A, Pijning T, Kralj S, Lopez CA, Eeuwema W, Dijkhuizen L, Dijkstra BW Proc Natl Acad Sci U S A. 2010 Nov 30. PMID:21118988<ref>PMID:21118988</ref>
	~~[[3klk]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index~~.~~html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http~~:~~//dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:021118988</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	[[Category: Lactobacillus reuteri]]		[[Category: Lactobacillus reuteri]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Dijkhuizen, L.]]		[[Category: Dijkhuizen, L]]
	[[Category: Dijkstra, B W.]]		[[Category: Dijkstra, B W]]
	[[Category: Eeuwema, W.]]		[[Category: Eeuwema, W]]
	[[Category: Kralj, S.]]		[[Category: Kralj, S]]
	[[Category: Pijning, T.]]		[[Category: Pijning, T]]
	[[Category: Vujicic-Zagar, A.]]		[[Category: Vujicic-Zagar, A]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Multidomain protein]]		[[Category: Multidomain protein]]

OCA at 02:43, 4 April 2013

2013-04-04T02:43:58Z

← Older revision		Revision as of 02:43, 4 April 2013
Line 1:		Line 1:
	~~[[Image:3klk.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3klk", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3klk\| PDB=3klk \| SCENE= }}		{{STRUCTURE_3klk\| PDB=3klk \| SCENE= }}

	===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===		===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21118988}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21118988 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21118988}}		{{ABSTRACT_PUBMED_21118988}}

	==About this Structure==		==About this Structure==
	[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].		[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21118988~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021118988</ref><references group="xtra"/><references/>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
			[[Category: Glucansucrase]]
	[[Category: Lactobacillus reuteri]]		[[Category: Lactobacillus reuteri]]
			[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Dijkhuizen, L.]]		[[Category: Dijkhuizen, L.]]
	[[Category: Dijkstra, B W.]]		[[Category: Dijkstra, B W.]]
Line 31:		Line 18:
	[[Category: Pijning, T.]]		[[Category: Pijning, T.]]
	[[Category: Vujicic-Zagar, A.]]		[[Category: Vujicic-Zagar, A.]]
			[[Category: Glycosyltransferase]]
			[[Category: Multidomain protein]]
			[[Category: Native form]]
			[[Category: Open conformation]]
			[[Category: Transferase]]

OCA: Protected "3klk" [edit=sysop:move=sysop]

2011-01-05T15:42:51Z

Protected "3klk" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:42, 5 January 2011
(No difference)

OCA at 15:42, 5 January 2011

2011-01-05T15:42:49Z

← Older revision		Revision as of 15:42, 5 January 2011
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	~~{{Seed}}~~
	[[Image:3klk.png\|left\|200px]]		[[Image:3klk.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
	~~3KLK~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].		[[3klk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].

	==Reference==		==Reference==
Line 32:		Line 31:
	[[Category: Pijning, T.]]		[[Category: Pijning, T.]]
	[[Category: Vujicic-Zagar, A.]]		[[Category: Vujicic-Zagar, A.]]
	~~[[Category: Glycosyltransferase]]~~
	~~[[Category: Multidomain protein]]~~
	~~[[Category: Native form]]~~
	~~[[Category: Open conformation]]~~
	~~[[Category: Transferase]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:25:10 2010''~~

OCA at 06:12, 22 December 2010

2010-12-22T06:12:33Z

← Older revision		Revision as of 06:12, 22 December 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
	[[Image:3klk.~~jpg~~\|left\|200px]]		[[Image:3klk.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===		===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_21118988}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 21118988 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_21118988}}

	==About this Structure==		==About this Structure==
	3KLK is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].		3KLK is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KLK OCA].

			==Reference==
			<ref group="xtra">PMID:21118988</ref><references group="xtra"/>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Lactobacillus reuteri]]		[[Category: Lactobacillus reuteri]]
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	[[Category: Kralj, S.]]		[[Category: Kralj, S.]]
	[[Category: Pijning, T.]]		[[Category: Pijning, T.]]
	[[Category: Zagar, A ~~Vujicic~~.]]		[[Category: Vujicic-Zagar, A.]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Multidomain protein]]		[[Category: Multidomain protein]]
Line 29:		Line 38:
	[[Category: Transferase]]		[[Category: Transferase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 3 11~~:09:01 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:25:10 2010''

OCA at 07:58, 3 November 2010

2010-11-03T07:58:23Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3klk is ON HOLD  until Paper Publication
+<!--
-Authors: Vujicic Zagar, A., Pijning, T., Kralj, S., Eeuwema, W., Dijkhuizen, L., Dijkstra, B.W.
+===Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo- form===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 25 09:13:14 2009''
+==About this Structure==

OCA at 07:13, 25 November 2009

2009-11-25T07:13:15Z

← Older revision		Revision as of 07:13, 25 November 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3klk is ON HOLD		The entry 3klk is ON HOLD until Paper Publication

	Authors: Vujicic Zagar, A., Pijning, T., Kralj, S., Eeuwema, W., Dijkhuizen, L., Dijkstra, B.W.		Authors: Vujicic Zagar, A., Pijning, T., Kralj, S., Eeuwema, W., Dijkhuizen, L., Dijkstra, B.W.
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	Description: Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo-form		Description: Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in triclinic apo-form

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov ~~18 18~~:58:36 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 25 09:13:14 2009''