3loo - Revision history

OCA at 10:21, 21 February 2024

2024-02-21T10:21:33Z

← Older revision		Revision as of 10:21, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3loo' size='340' side='right'caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3loo' size='340' side='right'caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anoga Anoga~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LOO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [https://pdbe.org/3loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [https://www.ebi.ac.uk/pdbsum/3loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [https://pdbe.org/3loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [https://www.ebi.ac.uk/pdbsum/3loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Genome analysis revealed a mosquito orthologue of adenosine kinase in Anopheles gambiae (AgAK; the most important vector for the transmission of Plasmodium falciparum in Africa). P. falciparum are purine auxotrophs and do not express an adenosine kinase but rely on their hosts for purines. AgAK was kinetically characterized and found to have the highest affinity for adenosine (Km 8.1 nM) of any known adenosine kinase. AgAK is specific for adenosine at the nucleoside site but several nucleotide triphosphate phosphoryl donors are tolerated. The AgAK crystal structure with bound bisubstrate analogue Ap4A (2.0 A resolution) reveals interactions for adenosine, ATP and the geometry for phosphoryl transfer. The polyphosphate charge is partly neutralized by a bound Mg2+ ion and an ion pair to a catalytic site Arg. The AgAK structure consists of a large catalytic core in a three-layered alpha/beta/alpha sandwich, and a small domain, termed the cap, in contact with adenosine. The specificity and tight-binding for adenosine arises from hydrogen bond interactions of Asn14, Leu16, Leu40, Leu133, Leu168, Phe168 and Thr171 and the backbone of Ile39 and Phe168 with the adenine ring as well as through hydrogen bond interactions between Asp18, Gly64 and Asn68 and the ribosyl 2'- and 3'-hydroxyl groups. With 48% sequence identity, the overall structure is more similar to human adenosine kinase than to AK from Toxoplasma gondii (31% identity). With this extraordinary affinity for AgAK, adenosine is efficiently captured and converted to its corresponding nucleotide suggesting an important role of this enzyme to maintain the adenosine nucleotide pool. mRNA analysis verifies that AgAK transcripts are produced in the adult insects.

	A High Affinity Adenosine Kinase from Anopheles gambiae.,Cassera MB, Ho MC, Merino EF, Burgos ES, Rinaldo-Matthis A, Almo SC, Schramm VL Biochemistry. 2011 Jan 19. PMID:21247194<ref>PMID:21247194</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3loo" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Adenosine kinase 3D structures\|Adenosine kinase 3D structures]]		*[[Adenosine kinase 3D structures\|Adenosine kinase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Adenosine kinase]]~~		[[Category: Anopheles gambiae]]
	~~[[Category: Anoga~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Almo~~, S C~~]]		[[Category: Almo SC]]
	[[Category: Cassera~~, M B~~]]		[[Category: Cassera MB]]
	[[Category: Ho, M C]]		[[Category: Ho M-C]]
	[[Category: Schramm~~, V L]]~~		[[Category: Schramm VL]]
	~~[[Category: Anopheles gambiae]]~~
	~~[[Category: Ap4a]]~~
	~~[[Category: P1]]~~
	~~[[Category: Transferase~~]]

OCA at 07:31, 20 July 2022

2022-07-20T07:31:22Z

← Older revision		Revision as of 07:31, 20 July 2022
Line 3:		Line 3:
	<StructureSection load='3loo' size='340' side='right'caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3loo' size='340' side='right'caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anoga Anoga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anoga Anoga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LOO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [~~http~~://pdbe.org/3loo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3loo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [https://pdbe.org/3loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [https://www.ebi.ac.uk/pdbsum/3loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:36, 14 October 2020

2020-10-14T06:36:55Z

← Older revision		Revision as of 06:36, 14 October 2020
Line 1:		Line 1:

	==Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate==		==Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate==
	<StructureSection load='3loo' size='340' side='right' caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3loo' size='340' side='right'caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anoga Anoga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anoga Anoga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3LOO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [http://pdbe.org/3loo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [http://www.ebi.ac.uk/pdbsum/3loo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [http://pdbe.org/3loo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [http://www.ebi.ac.uk/pdbsum/3loo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
	*[[Adenosine kinase\|Adenosine kinase]]		*[[Adenosine kinase 3D structures\|Adenosine kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	[[Category: Adenosine kinase]]		[[Category: Adenosine kinase]]
	[[Category: Anoga]]		[[Category: Anoga]]
			[[Category: Large Structures]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Cassera, M B]]		[[Category: Cassera, M B]]

OCA at 19:08, 5 August 2016

2016-08-05T19:08:45Z

← Older revision		Revision as of 19:08, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate==		==Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate==
	<StructureSection load='3loo' size='340' side='right' caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3loo' size='340' side='right' caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anopheles_gambiae Anopheles gambiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LOO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anoga Anoga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LOO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [http://www.ebi.ac.uk/pdbsum/3loo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [http://pdbe.org/3loo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [http://www.ebi.ac.uk/pdbsum/3loo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3loo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3loo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Adenosine kinase]]		[[Category: Adenosine kinase]]
	[[Category: ~~Anopheles gambiae~~]]		[[Category: Anoga]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Cassera, M B]]		[[Category: Cassera, M B]]
	[[Category: Ho, M C]]		[[Category: Ho, M C]]
	[[Category: Schramm, V L]]		[[Category: Schramm, V L]]
			[[Category: Anopheles gambiae]]
	[[Category: Ap4a]]		[[Category: Ap4a]]
	[[Category: P1]]		[[Category: P1]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 15:27, 18 December 2014

2014-12-18T15:27:23Z

← Older revision		Revision as of 15:27, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3loo\| PDB=3loo \| SCENE= }}~~		==Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate==
	===Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate===		<StructureSection load='3loo' size='340' side='right' caption='[[3loo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_21247194}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3loo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LOO FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B4P:BIS(ADENOSINE)-5-TETRAPHOSPHATE'>B4P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3loo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3loo RCSB], [http://www.ebi.ac.uk/pdbsum/3loo PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Genome analysis revealed a mosquito orthologue of adenosine kinase in Anopheles gambiae (AgAK; the most important vector for the transmission of Plasmodium falciparum in Africa). P. falciparum are purine auxotrophs and do not express an adenosine kinase but rely on their hosts for purines. AgAK was kinetically characterized and found to have the highest affinity for adenosine (Km 8.1 nM) of any known adenosine kinase. AgAK is specific for adenosine at the nucleoside site but several nucleotide triphosphate phosphoryl donors are tolerated. The AgAK crystal structure with bound bisubstrate analogue Ap4A (2.0 A resolution) reveals interactions for adenosine, ATP and the geometry for phosphoryl transfer. The polyphosphate charge is partly neutralized by a bound Mg2+ ion and an ion pair to a catalytic site Arg. The AgAK structure consists of a large catalytic core in a three-layered alpha/beta/alpha sandwich, and a small domain, termed the cap, in contact with adenosine. The specificity and tight-binding for adenosine arises from hydrogen bond interactions of Asn14, Leu16, Leu40, Leu133, Leu168, Phe168 and Thr171 and the backbone of Ile39 and Phe168 with the adenine ring as well as through hydrogen bond interactions between Asp18, Gly64 and Asn68 and the ribosyl 2'- and 3'-hydroxyl groups. With 48% sequence identity, the overall structure is more similar to human adenosine kinase than to AK from Toxoplasma gondii (31% identity). With this extraordinary affinity for AgAK, adenosine is efficiently captured and converted to its corresponding nucleotide suggesting an important role of this enzyme to maintain the adenosine nucleotide pool. mRNA analysis verifies that AgAK transcripts are produced in the adult insects.

	~~==About this Structure==~~		A High Affinity Adenosine Kinase from Anopheles gambiae.,Cassera MB, Ho MC, Merino EF, Burgos ES, Rinaldo-Matthis A, Almo SC, Schramm VL Biochemistry. 2011 Jan 19. PMID:21247194<ref>PMID:21247194</ref>
	~~[[3loo]] is a 3 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Anopheles_gambiae~~ Anopheles gambiae]. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il/oca-bin~~/~~ocashort?id=3LOO OCA].~~

	~~==Reference==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	<~~ref group="xtra"~~>~~PMID:021247194~~</~~ref~~>~~<references group~~=~~"xtra"/><references/>~~		</div>
	[[~~Category:~~ Adenosine kinase]]
	~~[[Category: Anopheles gambiae]]~~		==See Also==
	~~[[Category: Almo, S C.]]~~		*[[Adenosine kinase\|Adenosine kinase]]
	~~[[Category: Cassera, M B.]]~~		== References ==
	~~[[Category: Ho, M C.]]~~		<references/>
	~~[[Category: Schramm, V L.]]~~		__TOC__
			</StructureSection>
	[[Category: Adenosine kinase]]		[[Category: Adenosine kinase]]
	[[Category: Anopheles gambiae]]		[[Category: Anopheles gambiae]]
			[[Category: Almo, S C]]
			[[Category: Cassera, M B]]
			[[Category: Ho, M C]]
			[[Category: Schramm, V L]]
	[[Category: Ap4a]]		[[Category: Ap4a]]
	[[Category: P1]]		[[Category: P1]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 23:15, 10 April 2013

2013-04-10T23:15:52Z

@@ Line 1: / Line 1: @@
 {{STRUCTURE_3loo|  PDB=3loo  |  SCENE=  }}
 ===Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate===
 {{ABSTRACT_PUBMED_21247194}}
@@ Line 22: / Line 7: @@
 ==Reference==
-<ref group="xtra">PMID:21247194</ref><references group="xtra"/>
+<ref group="xtra">PMID:021247194</ref><references group="xtra"/><references/>
 [[Category: Adenosine kinase]]
 [[Category: Anopheles gambiae]]
@@ Line 29: / Line 14: @@
 [[Category: Ho, M C.]]
 [[Category: Schramm, V L.]]

OCA at 08:20, 6 April 2011

2011-04-06T08:20:05Z

← Older revision		Revision as of 08:20, 6 April 2011
Line 1:		Line 1:
	[[Image:3loo.~~jpg~~\|left\|200px]]		[[Image:3loo.png\|left\|200px]]

	<!--		<!--

OCA: Protected "3loo" [edit=sysop:move=sysop]

2011-02-02T06:29:29Z

Protected "3loo" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:29, 2 February 2011
(No difference)

OCA at 06:29, 2 February 2011

2011-02-02T06:29:25Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3loo.jpg|left|200px]]
-The entry 3loo is ON HOLD  until Paper Publication
+<!--
-Authors: Ho, M.-C., Cassera, M.B., Almo, S.C., Schramm, V.L.
+===Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:32:05 2010''
+<!--

OCA at 09:26, 30 June 2010

2010-06-30T09:26:30Z

← Older revision		Revision as of 09:26, 30 June 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3loo is ON HOLD		The entry 3loo is ON HOLD until Paper Publication

	Authors: Ho, M.-C., Cassera, M.B., Almo, S.C., Schramm, V.L.		Authors: Ho, M.-C., Cassera, M.B., Almo, S.C., Schramm, V.L.
Line 7:		Line 7:
	Description: Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate		Description: Crystal structure of Anopheles gambiae adenosine kinase in complex with P1,P4-di(adenosine-5) tetraphosphate

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Feb 25~~ 13:03:17 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:32:05 2010''