3m4e - Revision history

OCA at 10:23, 21 February 2024

2024-02-21T10:23:59Z

← Older revision		Revision as of 10:23, 21 February 2024
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	<StructureSection load='3m4e' size='340' side='right'caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3m4e' size='340' side='right'caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M4E FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3m3r\|3m3r]], [[3m4d\|3m4d]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900012:beta-cyclodextrin'>PRD_900012</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~hla, hly ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [https://pdbe.org/3m4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [https://www.ebi.ac.uk/pdbsum/3m4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [https://pdbe.org/3m4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [https://www.ebi.ac.uk/pdbsum/3m4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.		[https://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m4e ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m4e ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the alpha-hemolysin (alphaHL) pore that bind the adapter beta-cyclodextrin (betaCD) approximately 10(4) times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.

	Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.,Banerjee A, Mikhailova E, Cheley S, Gu LQ, Montoya M, Nagaoka Y, Gouaux E, Bayley H Proc Natl Acad Sci U S A. 2010 Apr 16. PMID:20400691<ref>PMID:20400691</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3m4e" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Gouaux, E~~]]		[[Category: Staphylococcus aureus]]
	[[Category: ~~Montoya, M~~]]		[[Category: Gouaux E]]
	[[Category: ~~Beta barrel]]~~		[[Category: Montoya M]]
	~~[[Category: Cell invasion]]~~
	~~[[Category: Cytolysis]]~~
	~~[[Category: Cytolytic protein]]~~
	~~[[Category: Hemolysis]]~~
	~~[[Category: Secreted]]~~
	~~[[Category: Toxin~~]]

OCA at 05:36, 6 October 2021

2021-10-06T05:36:38Z

← Older revision		Revision as of 05:36, 6 October 2021
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	==Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin==		==Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin==
	<StructureSection load='3m4e' size='340' side='right' caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3m4e' size='340' side='right'caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3M4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~BCD~~:~~BETA~~-~~CYCLODEXTRIN~~'>~~BCD~~</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m3r\|3m3r]], [[3m4d\|3m4d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3m3r\|3m3r]], [[3m4d\|3m4d]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hla, hly ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hla, hly ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [~~http~~://pdbe.org/3m4e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3m4e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [https://pdbe.org/3m4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [https://www.ebi.ac.uk/pdbsum/3m4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.		[[https://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Gouaux, E]]		[[Category: Gouaux, E]]
	[[Category: Montoya, M]]		[[Category: Montoya, M]]

OCA at 06:59, 9 January 2019

2019-01-09T06:59:32Z

← Older revision		Revision as of 06:59, 9 January 2019
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m4e_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m4e_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:57, 4 August 2016

2016-08-04T16:57:06Z

← Older revision		Revision as of 16:57, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin==		==Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin==
	<StructureSection load='3m4e' size='340' side='right' caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3m4e' size='340' side='right' caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCD:BETA-CYCLODEXTRIN'>BCD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCD:BETA-CYCLODEXTRIN'>BCD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m3r\|3m3r]], [[3m4d\|3m4d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m3r\|3m3r]], [[3m4d\|3m4d]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hla, hly ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hla, hly ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [http://www.ebi.ac.uk/pdbsum/3m4e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [http://pdbe.org/3m4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [http://www.ebi.ac.uk/pdbsum/3m4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m4e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m4e ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3m4e" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Staphylococcus aureus]]~~
	[[Category: Gouaux, E]]		[[Category: Gouaux, E]]
	[[Category: Montoya, M]]		[[Category: Montoya, M]]

OCA at 11:09, 25 December 2014

2014-12-25T11:09:53Z

← Older revision		Revision as of 11:09, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [http://www.ebi.ac.uk/pdbsum/3m4e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [http://www.ebi.ac.uk/pdbsum/3m4e PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU]] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:33, 9 December 2014

2014-12-09T08:33:52Z

← Older revision		Revision as of 08:33, 9 December 2014
Line 1:		Line 1:
	[[Image:~~3m4e~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin==
			<StructureSection load='3m4e' size='340' side='right' caption='[[3m4e]], [[Resolution\|resolution]] 2.30Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3m4e]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M4E FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCD:BETA-CYCLODEXTRIN'>BCD</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m3r\|3m3r]], [[3m4d\|3m4d]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hla, hly ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m4e RCSB], [http://www.ebi.ac.uk/pdbsum/3m4e PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m4e_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the alpha-hemolysin (alphaHL) pore that bind the adapter beta-cyclodextrin (betaCD) approximately 10(4) times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.

	~~{{STRUCTURE_3m4e\| PDB=3m4e \| SCENE= }}~~		Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.,Banerjee A, Mikhailova E, Cheley S, Gu LQ, Montoya M, Nagaoka Y, Gouaux E, Bayley H Proc Natl Acad Sci U S A. 2010 Apr 16. PMID:20400691<ref>PMID:20400691</ref>

	~~===Crystal structure~~ of the ~~M113N mutant of alpha-hemolysin bound to beta-cyclodextrin===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_20400691}}~~

	~~==About this Structure==~~
	~~[[3m4e]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA].~~

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin\|Hemolysin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:020400691<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Gouaux, E.]]		[[Category: Gouaux, E]]
	[[Category: Montoya, M.]]		[[Category: Montoya, M]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Cell invasion]]		[[Category: Cell invasion]]

OCA at 16:00, 20 October 2012

2012-10-20T16:00:59Z

← Older revision		Revision as of 16:00, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[3m4e]] is a 7 chain structure ~~of [[Hemolysin]]~~ with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA].		[[3m4e]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA].

	==See Also==		==See Also==

OCA: Protected "3m4e" [edit=sysop:move=sysop]

2012-07-26T20:19:12Z

Protected "3m4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 20:19, 26 July 2012
(No difference)

OCA at 20:19, 26 July 2012

2012-07-26T20:19:11Z

← Older revision		Revision as of 20:19, 26 July 2012
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	~~{{Seed}}~~
	[[Image:3m4e.png\|left\|200px]]		[[Image:3m4e.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3m4e", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3m4e\| PDB=3m4e \| SCENE= }}		{{STRUCTURE_3m4e\| PDB=3m4e \| SCENE= }}

	===Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin===		===Crystal structure of the M113N mutant of alpha-hemolysin bound to beta-cyclodextrin===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20400691}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_20400691}}		{{ABSTRACT_PUBMED_20400691}}

	==About this Structure==		==About this Structure==
	~~3M4E~~ is a 7 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA].		[[3m4e]] is a 7 chain structure of [[Hemolysin]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4E OCA].

			==See Also==
			*[[Hemolysin\|Hemolysin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20400691~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020400691</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Gouaux, E.]]		[[Category: Gouaux, E.]]
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	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Toxin]]		[[Category: Toxin]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 09:02:13 2010''~~

OCA at 06:02, 20 May 2010

2010-05-20T06:02:14Z

← Older revision		Revision as of 06:02, 20 May 2010
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	[[Image:3m4e.~~jpg~~\|left\|200px]]		[[Image:3m4e.png\|left\|200px]]

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	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ May ~~5 11~~:54:56 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 09:02:13 2010''