3mvb - Revision history

OCA at 10:26, 21 February 2024

2024-02-21T10:26:43Z

← Older revision		Revision as of 10:26, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3mvb' size='340' side='right'caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='3mvb' size='340' side='right'caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MVB FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3mva\|3mva]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.786Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">MTERF1 ([https://www.ncbi.nlm~~.~~nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [https://pdbe.org/3mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [https://www.ebi.ac.uk/pdbsum/3mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [https://pdbe.org/3mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [https://www.ebi.ac.uk/pdbsum/3mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~MTERF_HUMAN MTERF_HUMAN]~~] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. ~~Probably requires one or more components for~~ termination activity.<ref>PMID:20550934</ref>		[https://www.uniprot.org/uniprot/MTEF1_HUMAN MTEF1_HUMAN] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.<ref>PMID:20550934</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mvb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mvb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Defects in mitochondrial gene expression are associated with aging and disease. Mterf proteins have been implicated in modulating transcription, replication and protein synthesis. We have solved the structure of a member of this family, the human mitochondrial transcriptional terminator MTERF1, bound to dsDNA containing the termination sequence. The structure indicates that upon sequence recognition MTERF1 unwinds the DNA molecule, promoting eversion of three nucleotides. Base flipping is critical for stable binding and transcriptional termination. Additional structural and biochemical results provide insight into the DNA binding mechanism and explain how MTERF1 recognizes its target sequence. Finally, we have demonstrated that the mitochondrial pathogenic G3249A and G3244A mutations interfere with key interactions for sequence recognition, eliminating termination. Our results provide insight into the role of mterf proteins and suggest a link between mitochondrial disease and the regulation of mitochondrial transcription.

	Helix unwinding and base flipping enable human MTERF1 to terminate mitochondrial transcription.,Yakubovskaya E, Mejia E, Byrnes J, Hambardjieva E, Garcia-Diaz M Cell. 2010 Jun 11;141(6):982-93. PMID:20550934<ref>PMID:20550934</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3mvb" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Byrnes, J]]		[[Category: Byrnes J]]
	[[Category: Garcia-Diaz, M]]		[[Category: Garcia-Diaz M]]
	[[Category: Hambardjieva, E]]		[[Category: Hambardjieva E]]
	[[Category: Mejia, E]]		[[Category: Mejia E]]
	[[Category: Yakubovskaya, E]]		[[Category: Yakubovskaya E]]
	~~[[Category: All alpha-helix]]~~
	~~[[Category: Mitochondria]]~~
	~~[[Category: Protein-dna]]~~
	~~[[Category: Termination]]~~
	~~[[Category: Transcription factor]]~~
	~~[[Category: Transcription-dna complex~~]]

OCA at 05:42, 6 October 2021

2021-10-06T05:42:56Z

← Older revision		Revision as of 05:42, 6 October 2021
Line 1:		Line 1:

	==Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence==		==Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence==
	<StructureSection load='3mvb' size='340' side='right' caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='3mvb' size='340' side='right'caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MVB FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mva\|3mva]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3mva\|3mva]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTERF1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTERF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [~~http~~://pdbe.org/3mvb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3mvb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [https://pdbe.org/3mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [https://www.ebi.ac.uk/pdbsum/3mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>		[[https://www.uniprot.org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/3mvb_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/3mvb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Byrnes, J]]		[[Category: Byrnes, J]]
	[[Category: Garcia-Diaz, M]]		[[Category: Garcia-Diaz, M]]

OCA at 04:05, 5 August 2016

2016-08-05T04:05:48Z

← Older revision		Revision as of 04:05, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence==		==Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence==
	<StructureSection load='3mvb' size='340' side='right' caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>		<StructureSection load='3mvb' size='340' side='right' caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MVB FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mva\|3mva]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mva\|3mva]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTERF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTERF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [http://www.ebi.ac.uk/pdbsum/3mvb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [http://pdbe.org/3mvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [http://www.ebi.ac.uk/pdbsum/3mvb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mvb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3mvb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Byrnes, J]]		[[Category: Byrnes, J]]
	[[Category: Garcia-Diaz, M]]		[[Category: Garcia-Diaz, M]]

OCA at 15:42, 25 December 2014

2014-12-25T15:42:02Z

← Older revision		Revision as of 15:42, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [http://www.ebi.ac.uk/pdbsum/3mvb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [http://www.ebi.ac.uk/pdbsum/3mvb PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 15:54, 18 December 2014

2014-12-18T15:54:18Z

← Older revision		Revision as of 15:54, 18 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3mvb\| PDB=3mvb \| SCENE= }}~~		==Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence==
	===Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence===		<StructureSection load='3mvb' size='340' side='right' caption='[[3mvb]], [[Resolution\|resolution]] 2.79Å' scene=''>
	~~{{ABSTRACT_PUBMED_20550934}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MVB FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mva\|3mva]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MTERF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mvb RCSB], [http://www.ebi.ac.uk/pdbsum/3mvb PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/3mvb_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Defects in mitochondrial gene expression are associated with aging and disease. Mterf proteins have been implicated in modulating transcription, replication and protein synthesis. We have solved the structure of a member of this family, the human mitochondrial transcriptional terminator MTERF1, bound to dsDNA containing the termination sequence. The structure indicates that upon sequence recognition MTERF1 unwinds the DNA molecule, promoting eversion of three nucleotides. Base flipping is critical for stable binding and transcriptional termination. Additional structural and biochemical results provide insight into the DNA binding mechanism and explain how MTERF1 recognizes its target sequence. Finally, we have demonstrated that the mitochondrial pathogenic G3249A and G3244A mutations interfere with key interactions for sequence recognition, eliminating termination. Our results provide insight into the role of mterf proteins and suggest a link between mitochondrial disease and the regulation of mitochondrial transcription.

	~~==Function==~~		Helix unwinding and base flipping enable human MTERF1 to terminate mitochondrial transcription.,Yakubovskaya E, Mejia E, Byrnes J, Hambardjieva E, Garcia-Diaz M Cell. 2010 Jun 11;141(6):982-93. PMID:20550934<ref>PMID:20550934</ref>
	~~[[http://www~~.~~uniprot~~.~~org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA~~(~~Leu(uur~~)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[3mvb]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:020550934</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Byrnes, J.]]		[[Category: Byrnes, J]]
	[[Category: Garcia-Diaz, M.]]		[[Category: Garcia-Diaz, M]]
	[[Category: Hambardjieva, E.]]		[[Category: Hambardjieva, E]]
	[[Category: Mejia, E.]]		[[Category: Mejia, E]]
	[[Category: Yakubovskaya, E.]]		[[Category: Yakubovskaya, E]]
	[[Category: All alpha-helix]]		[[Category: All alpha-helix]]
	[[Category: Mitochondria]]		[[Category: Mitochondria]]

OCA: Protected "3mvb" [edit=sysop:move=sysop]

2013-04-17T20:55:26Z

Protected "3mvb" [edit=sysop:move=sysop]

← Older revision	Revision as of 20:55, 17 April 2013
(No difference)

OCA at 20:55, 17 April 2013

2013-04-17T20:55:23Z

← Older revision		Revision as of 20:55, 17 April 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	~~[[Image:3mvb.jpg\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3mvb", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3mvb\| PDB=3mvb \| SCENE= }}		{{STRUCTURE_3mvb\| PDB=3mvb \| SCENE= }}

	===Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence===		===Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence===
			{{ABSTRACT_PUBMED_20550934}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/MTERF_HUMAN MTERF_HUMAN]] Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.<ref>PMID:20550934</ref>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20550934}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~)~~, where 20550934 is~~ the ~~PubMed ID number~~.
	-->
	~~{{ABSTRACT_PUBMED_20550934}}~~

	==About this Structure==		==About this Structure==
	~~3MVB~~ is a 3 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA].		[[3mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVB OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20550934~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020550934</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Byrnes, J.]]		[[Category: Byrnes, J.]]
Line 36:		Line 23:
	[[Category: Transcription factor]]		[[Category: Transcription factor]]
	[[Category: Transcription-dna complex]]		[[Category: Transcription-dna complex]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:50:15 2010''~~

OCA at 09:44, 30 June 2010

2010-06-30T09:44:40Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 3mvb is ON HOLD
+<!--
-Authors: Yakubovskaya, E., Mejia, E., Byrnes, J., Hambardjieva, E., Garcia-Diaz, M.
+===Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:47:50 2010''
+<!--

OCA: New page: '''Unreleased structure''' The entry 3mvb is ON HOLD Authors: Yakubovskaya, E., Mejia, E., Byrnes, J., Hambardjieva, E., Garcia-Diaz, M. Description: Crystal structure of a triple RFY ...

2010-05-12T07:47:50Z

New page: '''Unreleased structure''' The entry 3mvb is ON HOLD Authors: Yakubovskaya, E., Mejia, E., Byrnes, J., Hambardjieva, E., Garcia-Diaz, M. Description: Crystal structure of a triple RFY ...

New page

'''Unreleased structure'''

The entry 3mvb is ON HOLD

Authors: Yakubovskaya, E., Mejia, E., Byrnes, J., Hambardjieva, E., Garcia-Diaz, M.

Description: Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:47:50 2010''