3o7o - Revision history

OCA at 09:30, 30 October 2024

2024-10-30T09:30:07Z

← Older revision		Revision as of 09:30, 30 October 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9S5X8_THEMA Q9S5X8_THEMA]		[https://www.uniprot.org/uniprot/Q9S5X8_THEMA Q9S5X8_THEMA]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein crystallization continues to be a major bottleneck in X-ray crystallography. Previous studies suggest that symmetric proteins, such as homodimers, might crystallize more readily than monomeric proteins or asymmetric complexes. Proteins that are naturally monomeric can be made homodimeric artificially. Our approach is to create homodimeric proteins by introducing single cysteines into the protein of interest, which are then oxidized to form a disulfide bond between the two monomers. By introducing the single cysteine at different sequence positions, one can produce a variety of synthetically dimerized versions of a protein, with each construct expected to exhibit its own crystallization behavior. In earlier work, we demonstrated the potential utility of the approach using T4 lysozyme as a model system. Here we report the successful application of the method to Thermotoga maritima CelA, a thermophilic endoglucanase enzyme with low sequence identity to proteins with structures previously reported in the Protein Data Bank. This protein had resisted crystallization in its natural monomeric form, despite a broad survey of crystallization conditions. The synthetic dimerization of the CelA mutant D188C yielded well-diffracting crystals with molecules in a packing arrangement that would not have occurred with native, monomeric CelA. A 2.4 A crystal structure was determined by single anomalous dispersion using a seleno-methionine derivatized protein. The results support the notion that synthetic symmetrization can be a useful approach for enlarging the search space for crystallizing monomeric proteins or asymmetric complexes.To determine the atomic structure of a protein by X-ray diffraction, a crystal of the molecule must be grown. This remains a challenge for many proteins. We show that by creating multiple dimeric versions of a protein of interest - which can be done by engineering a disulfide bond between two copies of the molecule -additional opportunities are created for the protein to crystallize. The method is used to determine the structure of an endoglucanase enzyme.

			Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima.,Forse GJ, Ram N, Banatao DR, Cascio D, Sawaya MR, Klock HE, Lesley SA, Yeates TO Protein Sci. 2010 Nov 16. PMID:21082721<ref>PMID:21082721</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:03, 14 February 2024

2024-02-14T10:03:50Z

← Older revision		Revision as of 10:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='3o7o' size='340' side='right'caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='3o7o' size='340' side='right'caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7O FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.41Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TM1524, TM_1524 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [https://pdbe.org/3o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [https://www.ebi.ac.uk/pdbsum/3o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [https://pdbe.org/3o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [https://www.ebi.ac.uk/pdbsum/3o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7o ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q9S5X8_THEMA Q9S5X8_THEMA]
	Protein crystallization continues to be a major bottleneck in X-ray crystallography. Previous studies suggest that symmetric proteins, such as homodimers, might crystallize more readily than monomeric proteins or asymmetric complexes. Proteins that are naturally monomeric can be made homodimeric artificially. Our approach is to create homodimeric proteins by introducing single cysteines into the protein of interest, which are then oxidized to form a disulfide bond between the two monomers. By introducing the single cysteine at different sequence positions, one can produce a variety of synthetically dimerized versions of a protein, with each construct expected to exhibit its own crystallization behavior. In earlier work, we demonstrated the potential utility of the approach using T4 lysozyme as a model system. Here we report the successful application of the method to Thermotoga maritima CelA, a thermophilic endoglucanase enzyme with low sequence identity to proteins with structures previously reported in the Protein Data Bank. This protein had resisted crystallization in its natural monomeric form, despite a broad survey of crystallization conditions. The synthetic dimerization of the CelA mutant D188C yielded well-diffracting crystals with molecules in a packing arrangement that would not have occurred with native, monomeric CelA. A 2.4 A crystal structure was determined by single anomalous dispersion using a seleno-methionine derivatized protein. The results support the notion that synthetic symmetrization can be a useful approach for enlarging the search space for crystallizing monomeric proteins or asymmetric complexes.To determine the atomic structure of a protein by X-ray diffraction, a crystal of the molecule must be grown. This remains a challenge for many proteins. We show that by creating multiple dimeric versions of a protein of interest - which can be done by engineering a disulfide bond between two copies of the molecule -additional opportunities are created for the protein to crystallize. The method is used to determine the structure of an endoglucanase enzyme.

	Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima.,Forse GJ, Ram N, Banatao DR, Cascio D, Sawaya MR, Klock HE, Lesley SA, Yeates TO Protein Sci. 2010 Nov 16. PMID:21082721<ref>PMID:~~21082721<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 43589]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Forse, G J~~]]		[[Category: Thermotoga maritima]]
	[[Category: ~~Ram, N~~]]		[[Category: Forse GJ]]
	[[Category: ~~Yeates, T O~~]]		[[Category: Ram N]]
	[[Category: ~~Disulfide bond]]~~		[[Category: Yeates TO]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Lattice contact]]~~
	~~[[Category: Macromolecular crystallization]]~~
	~~[[Category: Oligomer]]~~
	~~[[Category: Protein design]]~~
	~~[[Category: Synthetic symmetrization~~]]

OCA at 07:17, 12 May 2022

2022-05-12T07:17:51Z

← Older revision		Revision as of 07:17, 12 May 2022
Line 1:		Line 1:

	==Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima==		==Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima==
	<StructureSection load='3o7o' size='340' side='right' caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='3o7o' size='340' side='right'caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM1524, TM_1524 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM1524, TM_1524 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [~~http~~://pdbe.org/3o7o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o7o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [https://pdbe.org/3o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [https://www.ebi.ac.uk/pdbsum/3o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
			[[Category: Large Structures]]
	[[Category: Forse, G J]]		[[Category: Forse, G J]]
	[[Category: Ram, N]]		[[Category: Ram, N]]

OCA at 08:41, 8 November 2017

2017-11-08T08:41:03Z

← Older revision		Revision as of 08:41, 8 November 2017
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Glucanase\|Glucanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:07, 10 December 2016

2016-12-10T04:07:34Z

← Older revision		Revision as of 04:07, 10 December 2016
Line 1:		Line 1:

	==Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima==		==Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima==
	<StructureSection load='3o7o' size='340' side='right' caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='3o7o' size='340' side='right' caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM1524, TM_1524 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ~~Thermotoga maritima~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM1524, TM_1524 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [http://www.ebi.ac.uk/pdbsum/3o7o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [http://pdbe.org/3o7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [http://www.ebi.ac.uk/pdbsum/3o7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3o7o" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermotoga maritima~~]]		[[Category: Atcc 43589]]
	[[Category: Forse, G J]]		[[Category: Forse, G J]]
	[[Category: Ram, N]]		[[Category: Ram, N]]

OCA at 13:46, 9 December 2014

2014-12-09T13:46:49Z

← Older revision		Revision as of 13:46, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~3o7o~~.~~png~~\|~~left~~\|~~200px~~]]		==Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima==
			<StructureSection load='3o7o' size='340' side='right' caption='[[3o7o]], [[Resolution\|resolution]] 2.41Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7O FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM1524, TM_1524 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7o RCSB], [http://www.ebi.ac.uk/pdbsum/3o7o PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein crystallization continues to be a major bottleneck in X-ray crystallography. Previous studies suggest that symmetric proteins, such as homodimers, might crystallize more readily than monomeric proteins or asymmetric complexes. Proteins that are naturally monomeric can be made homodimeric artificially. Our approach is to create homodimeric proteins by introducing single cysteines into the protein of interest, which are then oxidized to form a disulfide bond between the two monomers. By introducing the single cysteine at different sequence positions, one can produce a variety of synthetically dimerized versions of a protein, with each construct expected to exhibit its own crystallization behavior. In earlier work, we demonstrated the potential utility of the approach using T4 lysozyme as a model system. Here we report the successful application of the method to Thermotoga maritima CelA, a thermophilic endoglucanase enzyme with low sequence identity to proteins with structures previously reported in the Protein Data Bank. This protein had resisted crystallization in its natural monomeric form, despite a broad survey of crystallization conditions. The synthetic dimerization of the CelA mutant D188C yielded well-diffracting crystals with molecules in a packing arrangement that would not have occurred with native, monomeric CelA. A 2.4 A crystal structure was determined by single anomalous dispersion using a seleno-methionine derivatized protein. The results support the notion that synthetic symmetrization can be a useful approach for enlarging the search space for crystallizing monomeric proteins or asymmetric complexes.To determine the atomic structure of a protein by X-ray diffraction, a crystal of the molecule must be grown. This remains a challenge for many proteins. We show that by creating multiple dimeric versions of a protein of interest - which can be done by engineering a disulfide bond between two copies of the molecule -additional opportunities are created for the protein to crystallize. The method is used to determine the structure of an endoglucanase enzyme.

	~~{{STRUCTURE_3o7o\| PDB=3o7o \| SCENE= }}~~		Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima.,Forse GJ, Ram N, Banatao DR, Cascio D, Sawaya MR, Klock HE, Lesley SA, Yeates TO Protein Sci. 2010 Nov 16. PMID:21082721<ref>PMID:21082721</ref>

	~~===Use~~ of ~~synthetic symmetrization in~~ the ~~crystallization and structure determination of CelA from Thermotoga maritima===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21082721}}~~

	~~==About this Structure==~~
	~~[[3o7o]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:021082721<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Forse, G J.]]		[[Category: Forse, G J]]
	[[Category: Ram, N.]]		[[Category: Ram, N]]
	[[Category: Yeates, T O.]]		[[Category: Yeates, T O]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 17:37, 20 October 2012

2012-10-20T17:37:24Z

← Older revision		Revision as of 17:37, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[3o7o]] is a 2 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].		[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].

	==See Also==		==See Also==

OCA at 01:30, 27 July 2012

2012-07-27T01:30:56Z

← Older revision		Revision as of 01:30, 27 July 2012
Line 1:		Line 1:
	[[Image:3o7o.png\|left\|200px]]		[[Image:3o7o.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3o7o", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3o7o\| PDB=3o7o \| SCENE= }}		{{STRUCTURE_3o7o\| PDB=3o7o \| SCENE= }}

	===Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima===		===Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21082721}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21082721 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21082721}}		{{ABSTRACT_PUBMED_21082721}}

	==About this Structure==		==About this Structure==
	[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].		[[3o7o]] is a 2 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].

			==See Also==
			*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21082721~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021082721</ref><references group="xtra"/>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Forse, G J.]]		[[Category: Forse, G J.]]
	[[Category: Ram, N.]]		[[Category: Ram, N.]]
	[[Category: Yeates, T O.]]		[[Category: Yeates, T O.]]
			[[Category: Disulfide bond]]
			[[Category: Hydrolase]]
			[[Category: Lattice contact]]
			[[Category: Macromolecular crystallization]]
			[[Category: Oligomer]]
			[[Category: Protein design]]
			[[Category: Synthetic symmetrization]]

OCA: Protected "3o7o" [edit=sysop:move=sysop]

2011-01-05T15:43:30Z

Protected "3o7o" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:43, 5 January 2011
(No difference)

OCA at 15:43, 5 January 2011

2011-01-05T15:43:29Z

← Older revision		Revision as of 15:43, 5 January 2011
Line 1:		Line 1:
	~~{{Seed}}~~		[[Image:3o7o.png\|left\|200px]]
	[[Image:3o7o.~~jpg~~\|left\|200px]]

	<!--		<!--
Line 20:		Line 19:

	==About this Structure==		==About this Structure==
	~~3O7O~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].		[[3o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7O OCA].

	==Reference==		==Reference==
Line 28:		Line 27:
	[[Category: Ram, N.]]		[[Category: Ram, N.]]
	[[Category: Yeates, T O.]]		[[Category: Yeates, T O.]]
	~~[[Category: Disulfide bond]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Lattice contact]]~~
	~~[[Category: Macromolecular crystallization]]~~
	~~[[Category: Oligomer]]~~
	~~[[Category: Protein design]]~~
	~~[[Category: Synthetic symmetrization]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 1 11:33:04 2010''~~