3o7v - Revision history

OCA at 02:12, 21 November 2024

2024-11-21T02:12:55Z

← Older revision		Revision as of 02:12, 21 November 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [https://pdbe.org/3o7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [https://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [https://pdbe.org/3o7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [https://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https://www~~.~~uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN] Plays~~ a ~~central~~ role ~~during spermatogenesis by repressing~~ transposable elements ~~and prevent their mobilization~~, ~~which is essential for~~ the ~~germline integrity~~. ~~Acts via the piRNA metabolic process~~, ~~which mediates~~ the ~~repression~~ of ~~transposable elements during meiosis by forming complexes composed of piRNAs and~~ Piwi ~~proteins and govern~~ the ~~methylation~~ and ~~subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24~~ to ~~30 nucleotide~~ RNAs ~~that are generated by a Dicer~~-~~independent mechanism~~ and ~~are primarily derived from transposons and other repeated sequence elements~~. ~~Besides their function in transposable elements repression~~, ~~piRNAs are probably involved in other processes during meiosis such as translation regulation~~. ~~Probable component of some RISC complex~~, which ~~mediates RNA cleavage and translational silencing. Also plays~~ a ~~role in the formation~~ of ~~chromatoid bodies and is required~~ for ~~some miRNAs stability~~ (~~By similarity~~). ~~Isoform 3 may be a negative developmental regulator~~.<ref>PMID:~~12037681~~</ref> <~~ref~~>~~PMID:14749716~~</~~ref~~> <~~ref~~>~~PMID:16287078~~</~~ref~~>		== Publication Abstract from PubMed ==
			Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

			Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.,Tian Y, Simanshu DK, Ma JB, Patel DJ Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193640<ref>PMID:21193640</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 3o7v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:03, 14 February 2024

2024-02-14T10:03:53Z

← Older revision		Revision as of 10:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='3o7v' size='340' side='right'caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3o7v' size='340' side='right'caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7V FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~>, ~~<scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [https://pdbe.org/3o7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [https://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [https://pdbe.org/3o7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [https://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>		[https://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

	Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.,Tian Y, Simanshu DK, Ma JB, Patel DJ Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193640<ref>PMID:21193640</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3o7v" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ma, J B]]		[[Category: Ma J-B]]
	[[Category: Patel~~, D J~~]]		[[Category: Patel DJ]]
	[[Category: Simanshu~~, D K~~]]		[[Category: Simanshu DK]]
	[[Category: Tian, Y]]		[[Category: Tian Y]]
	~~[[Category: Hili]]~~
	~~[[Category: Hiwi1]]~~
	~~[[Category: Paz domain]]~~
	~~[[Category: Pi-rna]]~~
	~~[[Category: Piwi]]~~
	~~[[Category: Rna binding protein]]~~
	~~[[Category: Rna binding protein-rna complex]]~~
	~~[[Category: Rna silencing~~]]

OCA at 07:17, 12 May 2022

2022-05-12T07:17:58Z

← Older revision		Revision as of 07:17, 12 May 2022
Line 1:		Line 1:

	==Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end==		==Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end==
	<StructureSection load='3o7v' size='340' side='right' caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3o7v' size='340' side='right'caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7V FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [~~http~~://pdbe.org/3o7v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [https://pdbe.org/3o7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [https://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>		[[https://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Ma, J B]]		[[Category: Ma, J B]]
	[[Category: Patel, D J]]		[[Category: Patel, D J]]

OCA at 18:31, 5 August 2016

2016-08-05T18:31:05Z

← Older revision		Revision as of 18:31, 5 August 2016
Line 1:		Line 1:

	==Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end==		==Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end==
	<StructureSection load='3o7v' size='340' side='right' caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3o7v' size='340' side='right' caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7V FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [http://www.ebi.ac.uk/pdbsum/3o7v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [http://pdbe.org/3o7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [http://www.ebi.ac.uk/pdbsum/3o7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3o7v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Ma, J B]]		[[Category: Ma, J B]]
	[[Category: Patel, D J]]		[[Category: Patel, D J]]

OCA at 04:50, 25 December 2014

2014-12-25T04:50:20Z

← Older revision		Revision as of 04:50, 25 December 2014
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	~~{{STRUCTURE_3o7v\| PDB=3o7v \| SCENE= }}~~		==Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end==
	===Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end===		<StructureSection load='3o7v' size='340' side='right' caption='[[3o7v]], [[Resolution\|resolution]] 2.10Å' scene=''>
	~~{{ABSTRACT_PUBMED_21193640}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3o7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7V FirstGlance]. <br>
	==Function==		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7x\|3o7x]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HIWI, Piwi-like protein 1, PIWIL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7v RCSB], [http://www.ebi.ac.uk/pdbsum/3o7v PDBsum]</span></td></tr>
			</table>
			== Function ==
	[[http://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>		[[http://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

	~~==About this Structure==~~		Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.,Tian Y, Simanshu DK, Ma JB, Patel DJ Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193640<ref>PMID:21193640</ref>
	~~[[3o7v]] is a~~ 2 ~~chain structure with sequence from [http:~~//en.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O7V OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:021193640</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ma, J B.]]		[[Category: Ma, J B]]
	[[Category: Patel, D J.]]		[[Category: Patel, D J]]
	[[Category: Simanshu, D K.]]		[[Category: Simanshu, D K]]
	[[Category: Tian, Y.]]		[[Category: Tian, Y]]
	[[Category: Hili]]		[[Category: Hili]]
	[[Category: Hiwi1]]		[[Category: Hiwi1]]

OCA at 11:54, 24 April 2013

2013-04-24T11:54:14Z

@@ Line 1: / Line 1: @@
 {{STRUCTURE_3o7v|  PDB=3o7v  |  SCENE=  }}
 ===Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end===
+==Function==
-<!--
+[[http://www.uniprot.org/uniprot/PIWL1_HUMAN PIWL1_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.<ref>PMID:12037681</ref> <ref>PMID:14749716</ref> <ref>PMID:16287078</ref>
 ==About this Structure==
@@ Line 22: / Line 10: @@
 ==Reference==
-<ref group="xtra">PMID:21193640</ref><references group="xtra"/>
+<ref group="xtra">PMID:021193640</ref><references group="xtra"/><references/>
 [[Category: Homo sapiens]]
 [[Category: Ma, J B.]]
@@ Line 28: / Line 16: @@
 [[Category: Simanshu, D K.]]
 [[Category: Tian, Y.]]

OCA at 05:57, 2 February 2011

2011-02-02T05:57:56Z

← Older revision		Revision as of 05:57, 2 February 2011
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	[[Image:3o7v.~~jpg~~\|left\|200px]]		[[Image:3o7v.png\|left\|200px]]

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OCA: Protected "3o7v" [edit=sysop:move=sysop]

2011-01-12T05:15:36Z

Protected "3o7v" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:15, 12 January 2011
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OCA at 05:15, 12 January 2011

2011-01-12T05:15:34Z

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-'''Unreleased structure'''
+[[Image:3o7v.jpg|left|200px]]
-The entry 3o7v is ON HOLD  until Paper Publication
+<!--
-Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.
+===Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct  6 05:47:33 2010''
+<!--

OCA at 02:38, 6 October 2010

2010-10-06T02:38:02Z

← Older revision		Revision as of 02:38, 6 October 2010
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3o7v is ON HOLD		The entry 3o7v is ON HOLD until Paper Publication

	Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.		Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.
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	Description: Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end		Description: Crystal Structure of human Hiwi1 (V361M) PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OCH3 at its 3'-end

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 11 23~~:38:34 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 05:47:33 2010''