3o7x - Revision history

OCA at 10:03, 14 February 2024

2024-02-14T10:03:57Z

← Older revision		Revision as of 10:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='3o7x' size='340' side='right'caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>		<StructureSection load='3o7x' size='340' side='right'caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7X FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9248Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PIWIL2, HILI ([https://www.ncbi.nlm~~.~~nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [https://pdbe.org/3o7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [https://www.ebi.ac.uk/pdbsum/3o7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [https://pdbe.org/3o7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [https://www.ebi.ac.uk/pdbsum/3o7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).		[https://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

	Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.,Tian Y, Simanshu DK, Ma JB, Patel DJ Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193640<ref>PMID:21193640</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 3o7x" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ma, J B]]		[[Category: Ma J-B]]
	[[Category: Patel~~, D J~~]]		[[Category: Patel DJ]]
	[[Category: Simanshu~~, D K~~]]		[[Category: Simanshu DK]]
	[[Category: Tian, Y]]		[[Category: Tian Y]]
	~~[[Category: Hili]]~~
	~~[[Category: Hiwi1]]~~
	~~[[Category: Paz domain]]~~
	~~[[Category: Pi-rna]]~~
	~~[[Category: Piwi]]~~
	~~[[Category: Rna binding protein]]~~
	~~[[Category: Rna silencing~~]]

OCA at 07:18, 12 May 2022

2022-05-12T07:18:02Z

← Older revision		Revision as of 07:18, 12 May 2022
Line 1:		Line 1:

	==Crystal structure of human Hili PAZ domain==		==Crystal structure of human Hili PAZ domain==
	<StructureSection load='3o7x' size='340' side='right' caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>		<StructureSection load='3o7x' size='340' side='right'caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7X FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIWIL2, HILI ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIWIL2, HILI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [~~http~~://pdbe.org/3o7x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o7x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [https://pdbe.org/3o7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [https://www.ebi.ac.uk/pdbsum/3o7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).		[[https://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Ma, J B]]		[[Category: Ma, J B]]
	[[Category: Patel, D J]]		[[Category: Patel, D J]]

OCA at 11:09, 5 August 2016

2016-08-05T11:09:08Z

← Older revision		Revision as of 11:09, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of human Hili PAZ domain==		==Crystal structure of human Hili PAZ domain==
	<StructureSection load='3o7x' size='340' side='right' caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>		<StructureSection load='3o7x' size='340' side='right' caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7X FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIWIL2, HILI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIWIL2, HILI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [http://www.ebi.ac.uk/pdbsum/3o7x PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [http://pdbe.org/3o7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [http://www.ebi.ac.uk/pdbsum/3o7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 3o7x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Ma, J B]]		[[Category: Ma, J B]]
	[[Category: Patel, D J]]		[[Category: Patel, D J]]

OCA at 18:18, 25 December 2014

2014-12-25T18:18:55Z

← Older revision		Revision as of 18:18, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_3o7x~~\| ~~PDB~~=3o7x \| ~~SCENE~~= }}		==Crystal structure of human Hili PAZ domain==
	===~~Crystal structure of human Hili PAZ domain~~===		<StructureSection load='3o7x' size='340' side='right' caption='[[3o7x]], [[Resolution\|resolution]] 2.92Å' scene=''>
	~~{{ABSTRACT_PUBMED_21193640}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[3o7x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7X FirstGlance]. <br>
	==Function==		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3i\|3o3i]], [[3o6e\|3o6e]], [[3o7v\|3o7v]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIWIL2, HILI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o7x RCSB], [http://www.ebi.ac.uk/pdbsum/3o7x PDBsum]</span></td></tr>
			</table>
			== Function ==
	[[http://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).		[[http://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Argonaute and Piwi proteins are key players in the RNA silencing pathway, with the former interacting with micro-RNAs (miRNAs) and siRNAs, whereas the latter targets piwi-interacting RNAs (piRNAs) that are 2'-O-methylated (2(')-OCH(3)) at their 3' ends. Germline-specific piRNAs and Piwi proteins play a critical role in genome defense against transposable elements, thereby protecting the genome against transposon-induced defects in gametogenesis and fertility. Humans contain four Piwi family proteins designated Hiwi1, Hiwi2, Hiwi3, and Hili. We report on the structures of Hili-PAZ (Piwi/Argonaute/Zwille) domain in the free state and Hiwi1 PAZ domain bound to self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' ends. These structures explain the molecular basis underlying accommodation of the 2(')-OCH(3) group within a preformed Hiwi1 PAZ domain binding pocket, whose hydrophobic characteristics account for the preferential binding of 2(')-OCH(3) over 2'-OH 3' ends. These results contrast with the more restricted binding pocket for the human Ago1 PAZ domain, which exhibits a reverse order, with preferential binding of 2'-OH over 2(')-OCH(3) 3' ends.

	~~==About this Structure==~~		Inaugural Article: Structural basis for piRNA 2'-O-methylated 3'-end recognition by Piwi PAZ (Piwi/Argonaute/Zwille) domains.,Tian Y, Simanshu DK, Ma JB, Patel DJ Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193640<ref>PMID:21193640</ref>
	~~[[3o7x]] is a 4 chain structure with sequence from [http~~://en.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O7X OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:021193640</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ma, J B.]]		[[Category: Ma, J B]]
	[[Category: Patel, D J.]]		[[Category: Patel, D J]]
	[[Category: Simanshu, D K.]]		[[Category: Simanshu, D K]]
	[[Category: Tian, Y.]]		[[Category: Tian, Y]]
	[[Category: Hili]]		[[Category: Hili]]
	[[Category: Hiwi1]]		[[Category: Hiwi1]]

OCA at 12:10, 24 April 2013

2013-04-24T12:10:45Z

← Older revision		Revision as of 12:10, 24 April 2013
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	~~[[Image:3o7x.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_3o7x", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_3o7x\| PDB=3o7x \| SCENE= }}		{{STRUCTURE_3o7x\| PDB=3o7x \| SCENE= }}

	===Crystal structure of human Hili PAZ domain===		===Crystal structure of human Hili PAZ domain===
			{{ABSTRACT_PUBMED_21193640}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/PIWL2_HUMAN PIWL2_HUMAN]] Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21193640}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov)~~, ~~where 21193640~~ is the ~~PubMed ID number~~.
	-->
	~~{{ABSTRACT_PUBMED_21193640}}~~

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
	<ref group="xtra">PMID:~~21193640~~</ref><references group="xtra"/>		<ref group="xtra">PMID:021193640</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ma, J B.]]		[[Category: Ma, J B.]]
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	[[Category: Simanshu, D K.]]		[[Category: Simanshu, D K.]]
	[[Category: Tian, Y.]]		[[Category: Tian, Y.]]
			[[Category: Hili]]
			[[Category: Hiwi1]]
			[[Category: Paz domain]]
			[[Category: Pi-rna]]
			[[Category: Piwi]]
			[[Category: Rna binding protein]]
			[[Category: Rna silencing]]

OCA at 05:58, 2 February 2011

2011-02-02T05:58:37Z

← Older revision		Revision as of 05:58, 2 February 2011
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	[[Image:3o7x.~~jpg~~\|left\|200px]]		[[Image:3o7x.png\|left\|200px]]

	<!--		<!--

OCA: Protected "3o7x" [edit=sysop:move=sysop]

2011-01-12T05:15:53Z

Protected "3o7x" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:15, 12 January 2011
(No difference)

OCA at 05:15, 12 January 2011

2011-01-12T05:15:51Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3o7x.jpg|left|200px]]
-The entry 3o7x is ON HOLD  until Paper Publication
+<!--
-Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.
+===Crystal structure of human Hili PAZ domain===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep  1 09:35:31 2010''
+<!--

OCA at 05:27, 1 September 2010

2010-09-01T05:27:23Z

← Older revision		Revision as of 05:27, 1 September 2010
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 3o7x is ON HOLD		The entry 3o7x is ON HOLD until Paper Publication

	Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.		Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.
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	Description: Crystal structure of human Hili PAZ domain		Description: Crystal structure of human Hili PAZ domain

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 11 23~~:38:36 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 1 09:35:31 2010''

OCA: New page: '''Unreleased structure''' The entry 3o7x is ON HOLD Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J. Description: Crystal structure of human Hili PAZ domain ''Page seeded by...

2010-08-11T19:31:18Z

New page: '''Unreleased structure''' The entry 3o7x is ON HOLD Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J. Description: Crystal structure of human Hili PAZ domain ''Page seeded by...

New page

'''Unreleased structure'''

The entry 3o7x is ON HOLD

Authors: Tian, Y., Simanshu, D.K., Ma, J.-B., Patel, D.J.

Description: Crystal structure of human Hili PAZ domain

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:38:36 2010''